Standard name
Human Ortholog
Description Transaldolase of unknown function; transcription is repressed by Mot1p and induced by alpha-factor and during diauxic shift; NQM1 has a paralog, TAL1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0.05
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0 0 0 0 0 0
Cytoplasm 0.67 0.27 0.65 0.75 0.84 0.76 0.73 0.72 0.62 0.69 0.62 0.56 0.94 0.89 0.81 0.87 0.86 0.92 0.44 0.13 0.42 0.76 0.76 0.67
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.3 0.06 0 0 0 0.2 0.33 0.29 0.51 0.36 0.48 0.37 0 0 0 0 0 0 0.1 0 0.05 0.05 0 0
Nucleus 0.09 0.55 0.22 0.16 0.09 0.1 0.06 0.08 0.06 0.08 0.11 0.15 0.06 0.1 0.17 0 0 0 0.17 0.57 0.27 0 0 0.06
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.08 0.21 0.1 0 0.05 0 0 0 0 0 0 0 0 0.05 0 0.1 0.12 0 0.16 0.17 0.17 0 0.05 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 2 2
Bud 1 0 1 0 1 0 3 4 6 6 4 15 0 0 0 0 1 0 1 3 0 0 2 11
Bud Neck 0 0 2 4 3 2 1 13 5 6 3 5 0 0 0 1 1 0 1 0 0 0 0 2
Bud Site 0 0 0 0 0 0 0 1 2 0 2 2 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 0 0 1 1 1 0 1 0 1 0 1 0 0 0 7 13 9 0 0 0 0 0 1
Cytoplasm 163 39 75 106 224 153 211 282 153 169 163 163 101 189 192 157 194 176 119 21 60 85 172 156
Endoplasmic Reticulum 1 1 0 0 0 0 0 0 1 0 0 0 0 1 0 8 14 10 1 0 0 0 1 1
Endosome 0 1 0 2 3 0 0 0 0 0 0 0 0 1 1 1 1 0 7 2 0 3 1 2
Golgi 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 3 0 1 2 0 1 1 0 0
Mitochondria 73 9 4 6 9 40 97 115 126 87 127 107 0 1 0 2 4 4 26 5 7 5 10 8
Nucleus 23 78 26 23 23 20 17 31 14 20 28 44 7 22 40 4 5 2 44 93 39 4 5 14
Nuclear Periphery 0 0 0 1 0 0 1 1 0 0 0 1 0 0 0 1 0 0 1 1 0 0 0 1
Nucleolus 0 2 1 1 0 0 0 0 2 1 3 10 0 0 1 0 0 0 0 0 0 0 1 1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 1
SpindlePole 1 0 1 0 3 0 2 7 5 2 2 7 0 0 0 3 2 3 7 3 2 2 6 5
Vac/Vac Membrane 20 30 12 6 14 8 11 7 1 3 5 10 0 10 6 19 26 8 42 28 24 4 11 15
Unique Cell Count 245 143 116 141 266 202 291 391 245 244 262 290 108 213 238 181 226 191 270 163 144 113 227 233
Labelled Cell Count 284 160 124 150 281 224 343 462 315 295 337 365 108 224 241 206 261 213 270 163 144 113 227 233


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.1 5.3 5.8 4.4 5.2 3.7 3.4 4.0 3.2 3.4 3.0 4.1 4.6 5.0 5.7 11.1 10.8 10.0 4.2 5.0 5.2
Std Deviation (1e-4) 0.6 1.4 7.8 1.5 2.6 1.4 1.6 3.5 1.6 1.5 1.2 2.9 1.2 1.6 8.7 6.1 6.3 6.1 1.2 1.9 2.0
Intensity Change (Log2) – – – -0.4 -0.15 -0.64 -0.75 -0.53 -0.86 -0.76 -0.94 -0.49 -0.32 -0.2 -0.01 0.95 0.91 0.8 -0.45 -0.19 -0.16


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.8 4.3 2.1 1.6 1.5 -0.4 0.9 -0.5 -1.6 5.3 5.2 3.3 4.5 4.5 6.0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus -1.2 -3.7 -3.1 -4.9 -4.3 -4.7 -3.8 -3.0 -1.7 -3.4 -3.0 -1.3 -5.6 -6.2 -6.3
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -1.9 -1.8 -2.3 -2.6 0 0 0 -3.7 -2.8 -3.4 -2.0 -3.1 0 0.3 -2.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.6215 0.049 -0.3662 -0.1287 0.0644 -0.2816 1.1493 0.3149 0.6159 0.0138 0.7833 0.0554 0.5185 -0.1815 0.3352 0.1547 -0.018 -0.2081
Actin 0.0447 0.0075 0.0087 0.0107 0.0263 0.0044 0.0832 0.0003 0.0316 0.0087 0.0222 0.005 0.0504 0.0008 0.0081 0.0538 0.0747 0.0017
Bud 0.0025 0.0006 0.0057 0.0004 0.0006 0.0002 0.0036 0.0028 0.0008 0.0008 0.0013 0.0001 0.0009 0.0087 0.0002 0.0003 0.0006 0.0003
Bud Neck 0.0151 0.0008 0.0006 0.0006 0.0019 0.0027 0.0132 0.0003 0.0009 0.0084 0.0007 0.0016 0.0042 0.0005 0.0004 0.0008 0.0008 0.002
Bud Periphery 0.0029 0.0004 0.0017 0.0005 0.0015 0.0004 0.012 0.0005 0.001 0.001 0.0042 0.0003 0.0011 0.0017 0.0003 0.0006 0.0009 0.0001
Bud Site 0.0151 0.0055 0.0091 0.0009 0.0009 0.0006 0.0173 0.0017 0.0049 0.0033 0.0013 0.0002 0.0158 0.0037 0.0008 0.0015 0.0008 0.0001
Cell Periphery 0.0011 0.0001 0.0002 0.0001 0.0002 0.0001 0.0013 0.0001 0.0001 0.0001 0.0001 0.0001 0.0005 0.0005 0.0001 0.0001 0.0002 0
Cytoplasm 0.0706 0.0589 0.0561 0.073 0.037 0.0336 0.0695 0.1087 0.0654 0.0387 0.0596 0.0827 0.0301 0.1032 0.039 0.0396 0.0773 0.0481
Cytoplasmic Foci 0.0323 0.0031 0.0057 0.0128 0.0227 0.002 0.0252 0.0019 0.012 0.0277 0.0214 0.0025 0.0371 0.0034 0.0083 0.0155 0.0173 0.0078
Eisosomes 0.0004 0.0001 0.0001 0.0003 0.0004 0.0001 0.0008 0.0001 0.0003 0.0001 0.0002 0.0001 0.0006 0 0.0002 0.0006 0.0002 0
Endoplasmic Reticulum 0.0047 0.0015 0.0014 0.0021 0.0063 0.0007 0.0055 0.0023 0.0028 0.0011 0.0012 0.001 0.0072 0.0014 0.0028 0.0032 0.0047 0.0009
Endosome 0.0307 0.0017 0.0027 0.0056 0.0465 0.0023 0.0192 0.0014 0.0267 0.026 0.018 0.0051 0.024 0.0008 0.0153 0.011 0.0305 0.0032
Golgi 0.0091 0.0003 0.0014 0.0013 0.0175 0.0007 0.0106 0.0001 0.0097 0.0234 0.0091 0.0021 0.014 0.0001 0.0055 0.0068 0.0159 0.0122
Lipid Particles 0.0133 0.0006 0.002 0.0154 0.0266 0.004 0.0087 0.0002 0.0157 0.0192 0.0471 0.0015 0.0229 0.0002 0.0179 0.0084 0.0581 0.0003
Mitochondria 0.0063 0.0008 0.0084 0.0143 0.0149 0.0014 0.0192 0.0029 0.0125 0.003 0.0715 0.0064 0.0051 0.0007 0.0117 0.0035 0.011 0.0007
None 0.1491 0.1988 0.2443 0.2627 0.1641 0.1521 0.1303 0.4093 0.2318 0.2278 0.1787 0.2528 0.0997 0.1952 0.1673 0.1545 0.0765 0.067
Nuclear Periphery 0.0288 0.0169 0.0313 0.015 0.0304 0.0082 0.0145 0.0082 0.0188 0.0106 0.0053 0.0065 0.0199 0.0144 0.0106 0.0234 0.022 0.0058
Nucleolus 0.0172 0.0137 0.008 0.0125 0.0231 0.017 0.0123 0.0057 0.0043 0.0144 0.0043 0.0083 0.005 0.005 0.0057 0.0047 0.0048 0.0144
Nucleus 0.5118 0.6208 0.5511 0.5068 0.4739 0.7108 0.5024 0.4192 0.5225 0.5473 0.4941 0.5956 0.6212 0.6392 0.676 0.6096 0.5685 0.7866
Peroxisomes 0.0138 0.0002 0.0013 0.0053 0.0376 0.0031 0.0182 0.0001 0.0069 0.0087 0.0486 0.0004 0.0172 0.0002 0.0046 0.0326 0.0074 0.0001
Punctate Nuclear 0.0214 0.0648 0.0583 0.0544 0.0618 0.0546 0.0212 0.0316 0.0236 0.0282 0.0088 0.0253 0.0196 0.0178 0.022 0.0266 0.01 0.0479
Vacuole 0.0059 0.0021 0.0012 0.0029 0.0029 0.0005 0.0085 0.0014 0.0052 0.001 0.0013 0.0015 0.0023 0.002 0.0024 0.002 0.0074 0.0006
Vacuole Periphery 0.0033 0.0007 0.0006 0.0023 0.0027 0.0003 0.0032 0.0013 0.0026 0.0006 0.0009 0.0009 0.001 0.0004 0.0009 0.001 0.0104 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 6.0566 2.9532 4.3828 3.0175 1.4278 4.4024 5.2255 6.3819 6.4873 4.3324
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –
Standard name
Human Ortholog
Description Transaldolase of unknown function; transcription is repressed by Mot1p and induced by alpha-factor and during diauxic shift; NQM1 has a paralog, TAL1, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (12%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Nqm1

Nqm1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Nqm1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available