Standard name
Human Ortholog
Description Zinc finger protein involved in control of meiosis; prevents meiosis by repressing IME1 expression and promotes mitosis by activating CLN2 expression; directly repressed by a1-alpha2 regulator; mediates cell type control of sporulation; relocalizes from nucleus to cytoplasm upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 0.77 0.78 0.9 0.47 0.75 0.72 0.8 0.79 0.57 0.66 0.55 0.65 0.89 0.98 0.99 – – – 0.6 0.57 0.62 0.76 0.84 0.8
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Mitochondria 0.08 0.12 0 0.08 0 0.19 0.21 0.16 0.54 0.36 0.49 0.4 0 0 0 – – – 0 0.05 0.06 0 0 0
Nucleus 0.12 0.09 0.05 0.39 0.1 0.13 0.06 0.05 0.05 0.06 0.08 0 0.14 0 0 – – – 0.15 0.12 0.08 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0.05 0 0 0 0 0.07
Vac/Vac Membrane 0.13 0.07 0.08 0.07 0.14 0.07 0 0.05 0 0 0 0 0 0 0 – – – 0.09 0.14 0.1 0.12 0.05 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 2 1 0 0 0 0 0 – – – 2 3 5 0 0 1
Bud 1 0 0 1 1 3 1 3 8 6 3 9 0 0 0 – – – 6 8 4 0 0 3
Bud Neck 1 4 3 4 6 7 10 15 8 12 10 9 0 1 0 – – – 1 4 0 0 0 0
Bud Site 0 0 0 2 0 1 1 0 5 3 5 2 0 0 0 – – – – – – – – –
Cell Periphery 0 1 1 0 1 0 2 2 1 2 2 1 0 0 0 – – – 0 1 1 0 0 0
Cytoplasm 157 347 247 97 229 258 180 391 261 279 294 341 25 203 199 – – – 134 274 182 38 132 149
Endoplasmic Reticulum 1 0 1 2 0 1 1 0 3 2 1 0 0 0 0 – – – 0 1 0 0 0 1
Endosome 1 0 1 1 5 2 0 3 1 0 1 1 0 0 0 – – – 3 2 3 1 1 2
Golgi 1 1 0 1 0 0 0 1 0 0 0 0 0 0 0 – – – 2 3 1 0 0 0
Mitochondria 16 52 3 16 13 70 47 79 249 152 262 211 0 0 0 – – – 4 23 18 0 4 5
Nucleus 25 42 13 80 32 48 14 24 23 25 44 23 4 0 1 – – – 33 57 22 0 2 0
Nuclear Periphery 0 0 0 0 0 0 0 1 0 1 1 0 0 1 0 – – – 0 1 1 0 0 0
Nucleolus 0 0 0 1 1 0 0 0 1 0 0 1 0 1 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 1 1 1 1 1 0 6 5 4 7 6 0 0 1 – – – 11 18 8 0 2 12
Vac/Vac Membrane 26 31 21 14 44 25 0 26 16 15 17 21 0 2 2 – – – 19 68 30 6 7 4
Unique Cell Count 205 447 275 207 307 360 224 492 459 423 537 521 28 207 200 224 482 292 51 158 188
Labelled Cell Count 229 479 291 220 333 416 256 551 583 502 647 625 29 208 203 224 482 292 51 158 188


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.1 3.4 5.0 4.8 5.0 4.0 3.0 4.3 3.1 3.5 3.5 3.6 4.4 4.7 4.3 – – – 5.4 5.5 5.4
Std Deviation (1e-4) 0.9 0.7 1.5 1.0 1.4 1.0 0.6 1.2 0.6 0.7 0.9 0.9 0.5 1.9 1.3 – – – 1.7 2.0 2.1
Intensity Change (Log2) – – – -0.07 0.0 -0.34 -0.76 -0.22 -0.69 -0.51 -0.54 -0.49 -0.18 -0.09 -0.22 – – – 0.1 0.12 0.11


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.7543 1.8749 1.2002 0.9787 0.8794 1.7646 2.6257 2.5788 2.7247 2.0435 2.2661 3.3025 0.6374 0.8477 0.5331 0.635 0.7044 0.9963
Actin 0.0801 0.0002 0.0065 0.0434 0.0013 0.0056 0.0131 0.0051 0.0083 0.0001 0.003 0.0008 0.0013 0.0002 0.0002 0.0002 0.0001 0.0002
Bud 0.0008 0.0013 0.0003 0.0004 0.0001 0.0002 0.001 0.0002 0.0022 0.0053 0.0156 0.0001 0.0001 0.0005 0.0016 0.001 0 0.0001
Bud Neck 0.0119 0.0006 0.0003 0.0002 0.0001 0.0048 0.0098 0.0004 0.0008 0.0003 0.0012 0.0026 0.0006 0.0004 0.0003 0.0005 0 0.002
Bud Periphery 0.0013 0.0019 0.0007 0.0007 0.0001 0.0005 0.0018 0.0002 0.0037 0.0118 0.0166 0.0001 0.0001 0.0003 0.0008 0.0026 0 0.0001
Bud Site 0.0179 0.0069 0.0005 0.0005 0.0002 0.0006 0.0044 0.0031 0.0023 0.0003 0.002 0.0001 0.0003 0.006 0.0019 0.0003 0 0.0001
Cell Periphery 0.0008 0.0004 0.0002 0.0001 0 0.0004 0.0006 0.0002 0.0002 0.0003 0.0004 0 0.0001 0.0001 0.0001 0.0001 0 0
Cytoplasm 0.0822 0.1474 0.0826 0.0663 0.0639 0.1246 0.1174 0.0527 0.063 0.0233 0.0565 0.0601 0.1433 0.1327 0.0759 0.0951 0.026 0.1331
Cytoplasmic Foci 0.0321 0.0023 0.007 0.0029 0.0055 0.0086 0.0196 0.0016 0.0074 0.0011 0.0037 0.0059 0.0075 0.0024 0.0079 0.0021 0.0034 0.0028
Eisosomes 0.0008 0.0002 0.0004 0.0003 0.0001 0.0003 0.0002 0.0001 0.0004 0.0001 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001 0 0
Endoplasmic Reticulum 0.0062 0.0028 0.0039 0.0008 0.0007 0.0014 0.0042 0.0016 0.0026 0.0008 0.0024 0.0009 0.0028 0.0019 0.0012 0.0021 0.0002 0.0013
Endosome 0.0232 0.0016 0.003 0.0005 0.0037 0.003 0.0072 0.0006 0.0043 0.0002 0.0107 0.0012 0.0029 0.0009 0.0008 0.001 0.0001 0.0012
Golgi 0.0103 0.0001 0.0026 0.0008 0.002 0.0015 0.0019 0.0001 0.0017 0 0.0009 0.0001 0.0002 0 0.0001 0 0 0
Lipid Particles 0.0211 0.0002 0.003 0.001 0.0171 0.007 0.003 0.0002 0.0024 0.0001 0.0015 0.0005 0.0038 0.0001 0.0003 0.0001 0.0001 0.0001
Mitochondria 0.0169 0.001 0.0015 0.0061 0.0041 0.0025 0.0017 0.0005 0.0024 0.0032 0.0017 0.0003 0.0013 0.0005 0.0009 0.0009 0.0001 0.0003
None 0.2691 0.5949 0.7924 0.8038 0.8334 0.3929 0.5559 0.417 0.817 0.8967 0.8123 0.5465 0.4075 0.6624 0.8012 0.8481 0.9676 0.4628
Nuclear Periphery 0.018 0.0076 0.0071 0.0015 0.0042 0.0039 0.0077 0.0076 0.0085 0.0028 0.0129 0.0032 0.0151 0.0054 0.0019 0.0031 0.0002 0.0039
Nucleolus 0.0025 0.0048 0.0009 0.0004 0.0002 0.0044 0.004 0.0059 0.001 0.0014 0.0026 0.0053 0.0033 0.0018 0.0017 0.0026 0.0001 0.0042
Nucleus 0.3357 0.2157 0.0711 0.0442 0.0332 0.4177 0.2082 0.4819 0.0608 0.0464 0.0429 0.352 0.3967 0.1751 0.0949 0.0343 0.0009 0.3689
Peroxisomes 0.0317 0.0001 0.0104 0.024 0.0281 0.0113 0.0047 0.0002 0.0021 0.0001 0.0008 0.0008 0.0002 0.0001 0.0005 0.0001 0.0001 0.0001
Punctate Nuclear 0.0281 0.0049 0.0036 0.0014 0.0014 0.0073 0.0222 0.0203 0.0062 0.0003 0.0054 0.0184 0.0109 0.007 0.0061 0.0029 0.0009 0.0176
Vacuole 0.0076 0.0046 0.0018 0.0008 0.0003 0.0013 0.0104 0.0005 0.0017 0.0036 0.0057 0.0006 0.0014 0.0017 0.0014 0.0022 0.0001 0.0009
Vacuole Periphery 0.0018 0.0005 0.0003 0.0002 0.0002 0.0003 0.0011 0.0001 0.0008 0.0018 0.0011 0.0001 0.0006 0.0002 0.0002 0.0005 0 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 5.4987 5.4617 8.9161 51.4318 34.0622 8.5488 5.7984 6.3362 33.0744 47.7485
Translational Efficiency 0.9361 0.542 1.3085 0.8159 1.0852 0.6931 0.5344 0.7449 1.1362 0.9136

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Zinc finger protein involved in control of meiosis; prevents meiosis by repressing IME1 expression and promotes mitosis by activating CLN2 expression; directly repressed by a1-alpha2 regulator; mediates cell type control of sporulation; relocalizes from nucleus to cytoplasm upon DNA replication stress
Localization
Cell Percentages cytoplasm (26%), nucleus (12%), mixed (43%)
Cell Cycle Regulation Yes
cytoplasm - MA (3.3e-05)
nucleus - T/G1 (2.4e-05)
Subcompartmental Group N/A

Rme1

Rme1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rme1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available