Standard name | |
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Human Ortholog | |
Description | Part of the heteromeric co-chaperone GimC/prefoldin complex; complex promotes efficient protein folding |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 1 | 1 | 6 | – | 5 | 5 | 5 | 6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 1 | 1 | 0 | 0 | 0 | 5 | 1 | – | 5 | 8 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 3 | 0 | 4 | 7 | 11 | 5 | 30 | – | 17 | 29 | 17 | 31 | 6 | 13 | 8 | 4 | 7 | 7 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 240 | 241 | 301 | 252 | 269 | 277 | 449 | – | 344 | 322 | 239 | 329 | 262 | 369 | 340 | 110 | 134 | 137 | 265 | 238 | 292 | 193 | 253 | 245 |
Endoplasmic Reticulum | 5 | 2 | 1 | 0 | 0 | 3 | 16 | – | 8 | 11 | 6 | 30 | 1 | 4 | 1 | 3 | 2 | 7 | 2 | 1 | 0 | 2 | 2 | 3 |
Endosome | 1 | 0 | 0 | 0 | 1 | 0 | 0 | – | 1 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 1 | 4 | 0 | 0 | – | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 2 | 1 | 0 | 0 | 0 | 1 | 0 |
Mitochondria | 0 | 0 | 2 | 2 | 6 | 39 | 11 | – | 64 | 64 | 57 | 56 | 0 | 0 | 1 | 4 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 |
Nucleus | 5 | 0 | 3 | 0 | 0 | 4 | 1 | – | 3 | 1 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 3 | 1 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 1 | 0 | 1 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 6 | 0 | 1 | 2 | 25 | 14 | 63 | – | 25 | 45 | 36 | 29 | 1 | 0 | 2 | 3 | 2 | 0 | 2 | 1 | 2 | 0 | 0 | 4 |
Unique Cell Count | 250 | 243 | 307 | 257 | 305 | 312 | 542 | 428 | 426 | 330 | 431 | 263 | 374 | 344 | 115 | 137 | 140 | 287 | 245 | 300 | 203 | 260 | 264 | |
Labelled Cell Count | 262 | 244 | 312 | 264 | 318 | 348 | 578 | 473 | 487 | 367 | 487 | 271 | 388 | 352 | 125 | 146 | 155 | 287 | 245 | 300 | 203 | 260 | 264 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 14.6 | 13.7 | 12.5 | 12.3 | 12.3 | 8.1 | 10.3 | – | 9.1 | 8.8 | 8.7 | 8.9 | 13.8 | 12.7 | 14.7 | 15.0 | 15.5 | 16.2 | 13.0 | 13.0 | 13.4 |
Std Deviation (1e-4) | 3.0 | 1.9 | 1.7 | 1.7 | 1.7 | 2.0 | 2.2 | – | 1.8 | 1.5 | 1.7 | 1.8 | 2.3 | 2.0 | 2.2 | 4.0 | 3.4 | 3.2 | 2.2 | 1.9 | 2.2 |
Intensity Change (Log2) | – | – | – | -0.02 | -0.02 | -0.62 | -0.27 | – | -0.46 | -0.51 | -0.52 | -0.49 | 0.15 | 0.03 | 0.24 | 0.26 | 0.31 | 0.38 | 0.06 | 0.06 | 0.1 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 1.2 | 1.8 | 0 | 3.0 | – | 2.1 | 3.5 | 2.7 | 3.7 | 0 | 1.8 | 1.0 | 0 | 0 | 0 |
Cytoplasm | 0 | -4.8 | -4.6 | -6.6 | – | -7.2 | -8.4 | -9.0 | -8.2 | 1.7 | 0.6 | 0.8 | -1.4 | -0.2 | -0.1 |
Endoplasmic Reticulum | 0 | 0 | 0 | 2.6 | – | 0 | 2.4 | 0 | 4.4 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 5.9 | 0 | – | 6.7 | 6.7 | 7.2 | 6.1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 4.8 | 3.4 | 6.0 | – | 4.0 | 5.6 | 5.7 | 4.3 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 8.6143 | 11.7056 | 10.3824 | 9.9231 | 9.327 | 10.4563 | 11.5456 | 14.2962 | 13.2496 | 12.3068 | 10.5314 | 12.8308 | 15.3223 | 18.381 | 16.6513 | 16.159 | 16.0879 | 16.5626 |
Actin | 0.0121 | 0.0002 | 0.0007 | 0.0001 | 0.0066 | 0.0001 | 0.0137 | 0.0001 | 0.0051 | 0.0003 | 0.0172 | 0.0079 | 0.0014 | 0.0002 | 0.001 | 0.0005 | 0.0001 | 0.0004 |
Bud | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0036 | 0.0001 | 0.0003 | 0.0006 | 0.0005 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0002 | 0 | 0.0001 |
Bud Neck | 0.0007 | 0.0001 | 0.0002 | 0.0002 | 0.0005 | 0.0004 | 0.0006 | 0.0001 | 0.0002 | 0.0002 | 0.0007 | 0.0005 | 0.0001 | 0.0001 | 0.0003 | 0.0003 | 0.0003 | 0.0007 |
Bud Periphery | 0.0003 | 0 | 0 | 0 | 0.0003 | 0 | 0.0013 | 0 | 0.0001 | 0.0002 | 0.0007 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0.0001 |
Bud Site | 0.0008 | 0.0002 | 0.0001 | 0 | 0.0002 | 0 | 0.0034 | 0.0001 | 0.0004 | 0.0001 | 0.0014 | 0 | 0.0001 | 0.0001 | 0.0013 | 0.0002 | 0 | 0 |
Cell Periphery | 0.0002 | 0 | 0 | 0 | 0.0001 | 0 | 0.0004 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 |
Cytoplasm | 0.8781 | 0.9934 | 0.9888 | 0.9886 | 0.9162 | 0.9892 | 0.8809 | 0.9955 | 0.9656 | 0.9716 | 0.8079 | 0.97 | 0.9759 | 0.9948 | 0.9844 | 0.98 | 0.9927 | 0.9857 |
Cytoplasmic Foci | 0.0129 | 0.0013 | 0.0014 | 0.0021 | 0.0091 | 0.0022 | 0.0104 | 0.0014 | 0.0068 | 0.0032 | 0.0279 | 0.0054 | 0.0032 | 0.0011 | 0.0025 | 0.0062 | 0.0015 | 0.0018 |
Eisosomes | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0.0003 | 0 | 0 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0046 | 0.0012 | 0.0038 | 0.0015 | 0.0042 | 0.0027 | 0.0037 | 0.0003 | 0.002 | 0.0128 | 0.0046 | 0.0017 | 0.0091 | 0.0009 | 0.0015 | 0.0035 | 0.0016 | 0.0028 |
Endosome | 0.0213 | 0.0007 | 0.0014 | 0.0031 | 0.0195 | 0.0018 | 0.0257 | 0.0005 | 0.0076 | 0.0042 | 0.0508 | 0.0083 | 0.0035 | 0.0008 | 0.003 | 0.0037 | 0.0013 | 0.002 |
Golgi | 0.0068 | 0.0001 | 0.0002 | 0.0001 | 0.0034 | 0.0001 | 0.0071 | 0.0001 | 0.0054 | 0.0001 | 0.0225 | 0.0015 | 0.0002 | 0.0002 | 0.0014 | 0.0005 | 0 | 0.0004 |
Lipid Particles | 0.0054 | 0 | 0 | 0 | 0.0009 | 0 | 0.0047 | 0 | 0.0004 | 0 | 0.0134 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 |
Mitochondria | 0.0138 | 0.0001 | 0.0001 | 0.0001 | 0.0055 | 0.0001 | 0.0152 | 0 | 0.0021 | 0.0001 | 0.0227 | 0.0005 | 0.0001 | 0 | 0.0018 | 0.0001 | 0.0001 | 0.0031 |
None | 0.009 | 0.0006 | 0.0007 | 0.0006 | 0.0013 | 0.0005 | 0.0032 | 0.0004 | 0.0007 | 0.0006 | 0.0025 | 0.0006 | 0.0021 | 0.0004 | 0.0004 | 0.0006 | 0.0003 | 0.0004 |
Nuclear Periphery | 0.0155 | 0.0005 | 0.0005 | 0.0008 | 0.0213 | 0.0005 | 0.007 | 0.0002 | 0.0005 | 0.0023 | 0.0077 | 0.0005 | 0.0013 | 0.0002 | 0.0003 | 0.0012 | 0.0004 | 0.0004 |
Nucleolus | 0.0005 | 0 | 0 | 0 | 0.0002 | 0 | 0.0012 | 0 | 0 | 0 | 0.0003 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0.0082 | 0.0013 | 0.0012 | 0.0015 | 0.0052 | 0.0015 | 0.008 | 0.0009 | 0.0011 | 0.0018 | 0.0042 | 0.0017 | 0.0017 | 0.0008 | 0.0008 | 0.0014 | 0.001 | 0.0015 |
Peroxisomes | 0.0036 | 0 | 0 | 0 | 0.0002 | 0 | 0.0019 | 0 | 0.0005 | 0 | 0.0072 | 0.0003 | 0.0001 | 0 | 0.0002 | 0.0001 | 0 | 0 |
Punctate Nuclear | 0.0022 | 0.0001 | 0.0001 | 0.0001 | 0.001 | 0.0001 | 0.0031 | 0 | 0.0002 | 0.0001 | 0.0028 | 0.0001 | 0.0003 | 0 | 0 | 0.0003 | 0 | 0 |
Vacuole | 0.0024 | 0.0002 | 0.0005 | 0.001 | 0.0015 | 0.0005 | 0.0029 | 0.0002 | 0.0008 | 0.0013 | 0.0026 | 0.0006 | 0.0007 | 0.0002 | 0.0004 | 0.0007 | 0.0003 | 0.0003 |
Vacuole Periphery | 0.001 | 0.0001 | 0.0001 | 0.0003 | 0.0025 | 0.0001 | 0.0013 | 0 | 0.0002 | 0.0003 | 0.002 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 60.6872 | 75.6222 | 69.5337 | 66.6657 | 92.9898 | 44.1117 | 61.0861 | 65.6298 | 72.3229 | 60.6179 |
Translational Efficiency | 1.9876 | 1.8345 | 1.7642 | 1.947 | 1.5469 | 2.1247 | 1.8214 | 1.7339 | 1.3362 | 1.7715 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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