Standard name
Human Ortholog
Description Twinfilin; highly conserved actin monomer-sequestering protein involved in regulation of the cortical actin cytoskeleton; coordinates actin filament severing and monomer sequestering at sites of rapid actin turnover; composed of two cofilin-like regions, stimulates actin depolymerization as does the mouse homolog, mTwf1
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.36 0.45 0.62 0.53 0.44 0.25 0.21 0.28 0.27 0.22 0.24 0.27 0.6 0.67 0.5 0.49 0.52 0.48 0.76 0.8 0.58 0.72 0.65 0.71
Bud 0.18 0.18 0.21 0.18 0.22 0.15 0.16 0.16 0.15 0.11 0.16 0.08 0.15 0.13 0.13 0.14 0.18 0.15 0.06 0.05 0.05 0.05 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0.09 0 0 0.05 0.05 0.06 0.06 0 0 0 0 0 0 0 0 0 0 0.05 – – – – – –
Cell Periphery 0 0 0 0.06 0 0.06 0 0 0 0 0 0 0.06 0.07 0.12 0 0 0.05 0 0 0 0 0 0
Cytoplasm 0 0.07 0 0.08 0.05 0 0.05 0 0 0 0 0 0.06 0.05 0 0.08 0.05 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0.07 0.09 0 0 0 0.05 0 0 0 0 0.05 0.06 0.05 0.09 0.07 0 0 0 0 0 0 0 0 0
Endosome 0 0 0.05 0 0 0.07 0.05 0.05 0 0 0 0 0 0 0.05 0 0.05 0 0 0 0.05 0 0 0
Golgi 0 0.15 0.31 0.28 0.2 0.15 0.09 0.09 0.1 0.08 0.05 0.11 0.34 0.46 0.51 0.5 0.46 0.41 0 0.06 0.08 0 0.06 0.07
Mitochondria 0.84 0.67 0.45 0.53 0.36 0.61 0.78 0.75 0.78 0.84 0.9 0.92 0.32 0.1 0.07 0.1 0.09 0.11 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.14 0.15 0.23 0.21 0.33 0.25 0.13 0.16 0.1 0.17 0.06 0.1 0.36 0.31 0.34 0.12 0.08 0.11 0.1 0 0.13 0.12 0.17 0.12
SpindlePole 0 0 0.05 0 0.05 0 0 0 0 0 0 0 0.05 0.05 0 0.07 0.07 0.05 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 44 46 52 81 95 56 65 82 84 82 46 32 132 192 147 157 117 125 98 95 75 107 170 187
Bud 22 19 18 27 47 34 49 46 46 42 31 10 32 37 38 46 41 39 7 6 6 7 7 7
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 4 0 0 1 0 1 2 1
Bud Site 11 2 0 7 10 13 18 12 9 7 2 1 7 2 0 4 7 13 – – – – – –
Cell Periphery 3 4 2 9 4 14 8 6 5 10 3 5 14 19 34 6 9 13 0 0 0 0 0 0
Cytoplasm 2 7 1 12 10 8 14 12 12 13 7 1 13 14 12 25 11 15 0 0 0 0 0 0
Endoplasmic Reticulum 9 9 3 3 4 12 9 11 11 14 10 7 11 25 21 4 5 3 0 1 0 0 0 1
Endosome 3 0 4 2 8 15 16 15 8 11 1 1 6 11 14 11 11 11 2 1 6 4 9 6
Golgi 4 15 26 43 44 34 28 27 32 31 9 13 74 132 150 158 103 107 3 6 9 3 16 19
Mitochondria 102 69 38 81 78 139 242 218 244 309 174 108 70 29 21 33 20 29 3 2 4 3 6 7
Nucleus 0 1 1 2 0 2 3 4 3 2 1 2 0 0 0 0 1 1 0 0 0 0 0 0
Nuclear Periphery 0 0 0 2 2 3 7 4 3 4 3 1 2 1 2 1 0 1 0 0 1 0 0 0
Nucleolus 0 1 2 1 0 3 4 1 5 1 2 3 2 0 0 6 3 7 0 0 0 0 0 0
Peroxisomes 17 15 19 32 71 56 40 48 30 61 11 12 78 87 101 39 19 28 13 1 16 18 43 30
SpindlePole 1 0 4 3 11 10 3 12 4 7 1 2 10 13 12 23 15 13 0 0 5 1 1 0
Vac/Vac Membrane 0 0 2 0 2 1 3 0 3 4 0 2 3 5 0 7 1 1 0 1 0 0 0 0
Unique Cell Count 122 103 84 154 215 227 310 291 311 369 193 118 219 285 295 318 225 260 130 119 130 149 261 265
Labelled Cell Count 218 188 172 305 386 400 509 498 499 598 301 201 454 567 552 520 367 406 130 119 130 149 261 265


Actin

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.6 10.4 9.0 8.8 8.6 8.0 7.5 7.6 7.6 7.4 7.0 7.3 9.5 11.1 11.3 17.2 17.3 16.8 9.2 9.7 10.7
Std Deviation (1e-4) 1.8 2.3 2.4 1.7 1.6 2.1 1.8 1.8 2.0 1.8 1.8 2.0 2.2 2.5 2.4 6.0 5.2 5.3 2.0 2.0 1.9
Intensity Change (Log2) – – – -0.03 -0.06 -0.17 -0.25 -0.24 -0.25 -0.27 -0.36 -0.29 0.08 0.3 0.33 0.94 0.95 0.91 0.04 0.12 0.26


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -1.4 -2.8 -6.1 -7.3 -5.7 -6.0 -7.2 -6.1 -4.9 -0.3 0.9 -2.0 -2.0 -1.6 -2.2
Bud -0.7 0.1 -1.4 -1.2 -1.2 -1.5 -2.5 -1.1 -2.6 -1.4 -1.9 -1.9 -1.6 -0.6 -1.4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 2.5 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 2.2 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 1.6 1.2 0 0 0
Endosome 0 0 0.6 0 0 0 0 0 0 0 0 0 0 0 0
Golgi -0.5 -1.9 -3.2 -5.2 -5.0 -4.7 -5.6 -6.1 -3.5 0.5 2.5 3.2 3.1 2.4 1.7
Mitochondria 1.1 -1.4 2.5 5.9 5.1 6.0 7.5 8.1 7.2 -2.2 -7.3 -8.5 -7.5 -7.3 -6.9
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes -0.3 1.8 0.4 -2.2 -1.3 -3.2 -1.3 -4.2 -2.4 2.2 1.4 2.0 -2.4 -3.4 -2.7
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0.8 0.6 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.4711 3.5887 3.9178 3.9245 3.4967 3.9338 4.5799 4.4753 4.3838 4.0494 3.9781 4.5578 5.8166 4.4784 4.8109 4.6881 4.9297 5.2614
Actin 0.8587 0.7543 0.8153 0.8164 0.8924 0.8461 0.6302 0.7849 0.8427 0.8742 0.733 0.9216 0.8645 0.86 0.8514 0.8955 0.8997 0.8991
Bud 0.0007 0.0092 0.0379 0.0515 0.0083 0.0035 0.0019 0.0017 0.0237 0.013 0.021 0.0008 0.0009 0.002 0.0183 0.0034 0.0023 0.0003
Bud Neck 0.0011 0.0012 0.0021 0.0007 0.0151 0.0049 0.0089 0.0059 0.0035 0.0014 0.0005 0.0181 0.0015 0.0051 0.0114 0.0004 0.001 0.0038
Bud Periphery 0.0008 0.0048 0.0649 0.0981 0.0223 0.0056 0.0019 0.0013 0.0332 0.0259 0.0634 0.0014 0.0009 0.0014 0.0315 0.0062 0.0034 0.0004
Bud Site 0.0036 0.0515 0.0239 0.0073 0.0192 0.0036 0.0177 0.0645 0.0291 0.0047 0.0035 0.0011 0.0086 0.0733 0.0268 0.0023 0.0018 0.0012
Cell Periphery 0.0001 0.0002 0.0006 0.0009 0.0011 0.0001 0.0006 0.0001 0.0006 0.0003 0.0003 0.0002 0.0001 0.0002 0.0004 0.0001 0.0001 0.0001
Cytoplasm 0.0009 0.0119 0.0016 0.0025 0.0005 0.0004 0.0083 0.0265 0.0095 0.0009 0.0011 0.0002 0.0008 0.004 0.0013 0.0004 0.0044 0.0001
Cytoplasmic Foci 0.0247 0.0515 0.0056 0.0021 0.0053 0.0137 0.0593 0.037 0.0131 0.0034 0.0391 0.0077 0.0198 0.0122 0.0101 0.0067 0.0032 0.0078
Eisosomes 0.0005 0.0003 0.0002 0.0004 0.0006 0.0007 0.0006 0.0003 0.0004 0.0005 0.0004 0.0008 0.0005 0.0003 0.0003 0.0004 0.0003 0.0004
Endoplasmic Reticulum 0.0005 0.001 0.0001 0.0005 0.0006 0.0003 0.0041 0.0004 0.0013 0.0018 0.0002 0.0004 0.0009 0.0002 0.0039 0.0002 0.0027 0
Endosome 0.0121 0.0112 0.0011 0.0009 0.0038 0.0147 0.0495 0.0097 0.0069 0.0237 0.0119 0.0037 0.0198 0.0108 0.0047 0.01 0.0129 0.0026
Golgi 0.0407 0.0366 0.0066 0.0038 0.0067 0.0396 0.0344 0.0177 0.0125 0.015 0.0215 0.0235 0.0396 0.0041 0.0172 0.0381 0.0029 0.0416
Lipid Particles 0.0049 0.0018 0.0019 0.0002 0.0026 0.0094 0.023 0.0043 0.0021 0.0052 0.0219 0.0042 0.0068 0.0003 0.0021 0.0008 0.0015 0.0015
Mitochondria 0.0034 0.0061 0.0104 0.0059 0.0026 0.0226 0.0101 0.002 0.0034 0.0144 0.0565 0.0011 0.0091 0.0007 0.0046 0.0207 0.0018 0.0146
None 0.0006 0.0016 0.0001 0.0006 0.0002 0.0001 0.0262 0.0004 0.0013 0.0009 0.0032 0.0002 0.0007 0.0004 0.0003 0.0001 0.0013 0
Nuclear Periphery 0.0099 0.0002 0.0001 0.0005 0.0006 0.0003 0.0135 0.0001 0.0003 0.0025 0.0003 0.0005 0.0018 0.0001 0.0032 0.0001 0.0282 0
Nucleolus 0.0002 0.0003 0 0.0001 0.0005 0 0.0052 0 0.0001 0.0001 0.0003 0 0.0003 0.0002 0.0001 0 0.001 0.0001
Nucleus 0.0017 0.0004 0.0001 0.0004 0.0005 0.0001 0.0077 0.0001 0.0001 0.0002 0.0002 0.0001 0.0003 0.0005 0.0025 0 0.0118 0
Peroxisomes 0.0317 0.0541 0.0271 0.0063 0.0142 0.0333 0.0679 0.0417 0.0153 0.0091 0.0192 0.0131 0.0169 0.0231 0.0084 0.0141 0.0055 0.0261
Punctate Nuclear 0.002 0.0006 0.0001 0.0001 0.0003 0.0003 0.0239 0.0006 0.0003 0.0004 0.0007 0.0011 0.0041 0.0006 0.0008 0.0001 0.0119 0.0001
Vacuole 0.0004 0.0008 0.0002 0.0006 0.0023 0.0003 0.0036 0.0003 0.0004 0.001 0.0007 0.0001 0.0011 0.0004 0.0004 0.0002 0.0013 0
Vacuole Periphery 0.0007 0.0003 0.0001 0.0002 0.0003 0.0003 0.0015 0.0002 0.0002 0.0013 0.0009 0.0001 0.0011 0.0001 0.0003 0.0002 0.0011 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 17.1199 12.8357 20.5147 35.2268 29.3569 19.8702 25.9564 37.059 31.6546 28.3036
Translational Efficiency 2.2686 3.712 2.3111 1.3807 1.4422 2.7746 2.0076 1.4472 2.0692 1.8249

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Twinfilin; highly conserved actin monomer-sequestering protein involved in regulation of the cortical actin cytoskeleton; coordinates actin filament severing and monomer sequestering at sites of rapid actin turnover; composed of two cofilin-like regions, stimulates actin depolymerization as does the mouse homolog, mTwf1
Localization
Cell Percentages actin (85%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Twf1

Twf1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Twf1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available