Standard name | |
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Human Ortholog | |
Description | Splicing factor; component of the U4/U6-U5 snRNP complex |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 2 | 1 | 0 | 4 | 5 | 3 | 4 | 5 | 4 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 4 | 2 | 0 | 2 | 7 |
Bud Neck | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 |
Bud Site | 1 | 0 | 0 | 0 | 0 | 3 | 11 | 9 | 5 | 7 | 12 | 25 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 3 | 19 | 8 | 6 | 15 | 16 | 26 | 24 | 9 | 17 | 10 | 16 | 20 | 36 | 70 | 16 | 13 | 17 | 0 | 4 | 1 | 3 | 1 | 5 |
Endoplasmic Reticulum | 1 | 1 | 1 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 5 | 6 | 5 | 0 | 1 | 0 | 0 | 0 | 0 |
Endosome | 0 | 4 | 2 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 3 | 2 | 4 | 1 | 5 | 0 | 0 | 6 | 5 |
Golgi | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 2 | 2 | 2 | 0 | 0 | 0 |
Mitochondria | 62 | 13 | 31 | 42 | 68 | 174 | 233 | 237 | 189 | 288 | 191 | 227 | 7 | 3 | 3 | 7 | 0 | 2 | 0 | 3 | 3 | 1 | 1 | 7 |
Nucleus | 173 | 432 | 232 | 202 | 250 | 262 | 321 | 341 | 253 | 305 | 232 | 283 | 166 | 185 | 248 | 92 | 100 | 101 | 174 | 432 | 222 | 88 | 186 | 146 |
Nuclear Periphery | 3 | 4 | 0 | 0 | 0 | 2 | 8 | 3 | 3 | 4 | 2 | 4 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 3 | 5 | 1 | 0 | 1 | 8 | 19 | 16 | 15 | 29 | 19 | 26 | 0 | 0 | 2 | 1 | 0 | 1 | 0 | 4 | 3 | 2 | 16 | 18 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 2 | 0 | 1 | 1 | 2 | 1 | 0 | 0 | 1 | 5 | 4 | 1 | 0 | 3 | 8 |
Vac/Vac Membrane | 1 | 39 | 8 | 5 | 19 | 7 | 11 | 5 | 2 | 4 | 4 | 5 | 3 | 5 | 11 | 11 | 15 | 14 | 9 | 36 | 34 | 7 | 24 | 56 |
Unique Cell Count | 189 | 480 | 258 | 226 | 309 | 347 | 444 | 458 | 331 | 428 | 306 | 374 | 193 | 230 | 326 | 120 | 129 | 133 | 198 | 508 | 276 | 109 | 249 | 270 |
Labelled Cell Count | 249 | 520 | 284 | 258 | 358 | 474 | 633 | 642 | 481 | 660 | 475 | 591 | 197 | 235 | 339 | 137 | 137 | 150 | 198 | 508 | 276 | 109 | 249 | 270 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.3 | 5.9 | 5.1 | 4.6 | 4.6 | 4.3 | 3.8 | 4.0 | 3.9 | 3.7 | 3.8 | 3.8 | 5.4 | 5.3 | 5.3 | 7.4 | 8.3 | 8.6 | 4.8 | 5.6 | 6.6 |
Std Deviation (1e-4) | 0.8 | 1.3 | 1.3 | 1.1 | 1.3 | 1.2 | 1.1 | 1.2 | 0.8 | 1.2 | 0.9 | 1.3 | 1.0 | 1.3 | 1.4 | 1.8 | 1.9 | 1.9 | 1.2 | 1.6 | 1.8 |
Intensity Change (Log2) | – | – | – | -0.13 | -0.16 | -0.25 | -0.41 | -0.34 | -0.37 | -0.45 | -0.4 | -0.44 | 0.09 | 0.06 | 0.05 | 0.54 | 0.7 | 0.76 | -0.07 | 0.15 | 0.38 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.3 | 1.1 | 0.9 | 1.6 | 1.3 | -0.3 | 0.6 | 0.1 | 0.8 | 3.2 | 4.8 | 6.5 | 3.8 | 2.9 | 3.7 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -3.2 | -4.6 | -5.7 | -1.9 | -4.1 | -3.5 |
Nucleus | -0.2 | -3.0 | -4.5 | -5.5 | -5.0 | -4.3 | -5.7 | -4.4 | -4.5 | -1.3 | -3.0 | -4.3 | -3.4 | -3.3 | -3.7 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -0.6 | 1.7 | -0.8 | -0.5 | 0 | 0 | 0 | -1.5 | -1.5 | 0 | -0.6 | 0.2 | 2.5 | 3.3 | 3.0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.2285 | 1.2896 | 0.1366 | -0.2878 | 0.1337 | 0.7351 | 3.3378 | 3.3946 | 3.3175 | 2.9582 | 3.5595 | 3.4437 | 4.2386 | 4.6659 | 4.3565 | 4.6145 | 4.2344 | 4.7111 |
Actin | 0.0045 | 0 | 0.0003 | 0.0002 | 0.0135 | 0.001 | 0.01 | 0 | 0.0074 | 0.0234 | 0.002 | 0.0005 | 0.001 | 0 | 0.0053 | 0.0006 | 0 | 0.0062 |
Bud | 0 | 0 | 0.0003 | 0.0041 | 0.0001 | 0.002 | 0.0007 | 0 | 0.004 | 0.0005 | 0.0005 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0 | 0 |
Bud Neck | 0.0003 | 0.0001 | 0.0003 | 0.0008 | 0.0016 | 0.001 | 0.0018 | 0.0001 | 0.0065 | 0.0075 | 0.0009 | 0.0012 | 0.0009 | 0.0001 | 0.0009 | 0.0009 | 0.0005 | 0.0007 |
Bud Periphery | 0 | 0 | 0.0001 | 0.0011 | 0.0002 | 0.0006 | 0.0027 | 0 | 0.002 | 0.0009 | 0.0026 | 0.0004 | 0.0001 | 0.0001 | 0.0003 | 0.0003 | 0 | 0 |
Bud Site | 0.0002 | 0 | 0.0006 | 0.0016 | 0.0003 | 0 | 0.0013 | 0 | 0.0059 | 0.0037 | 0.0008 | 0.0001 | 0.0002 | 0.0002 | 0.0004 | 0.0004 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0.0003 | 0 | 0 | 0.0004 | 0 | 0.0009 | 0.0006 | 0.0003 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 |
Cytoplasm | 0.004 | 0.0008 | 0.0059 | 0.0215 | 0.0029 | 0.0039 | 0.0058 | 0.0005 | 0.0116 | 0.0016 | 0.0033 | 0.0006 | 0.0021 | 0.0015 | 0.0054 | 0.0005 | 0.0005 | 0.0028 |
Cytoplasmic Foci | 0.0023 | 0 | 0.0005 | 0.0199 | 0.0007 | 0.0003 | 0.003 | 0 | 0.008 | 0.0048 | 0.0076 | 0.0002 | 0.0002 | 0.0025 | 0.0018 | 0.0001 | 0 | 0.0021 |
Eisosomes | 0.0003 | 0 | 0.0002 | 0 | 0.0005 | 0.0001 | 0.0004 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0 |
Endoplasmic Reticulum | 0.0004 | 0 | 0.0004 | 0.0006 | 0.0004 | 0.0003 | 0.0045 | 0.0001 | 0.0019 | 0.0003 | 0.0042 | 0.0004 | 0.0024 | 0.0001 | 0.0013 | 0.0005 | 0 | 0.0001 |
Endosome | 0.0029 | 0 | 0.0005 | 0.0011 | 0.0014 | 0.0004 | 0.0119 | 0.0001 | 0.0068 | 0.0022 | 0.0335 | 0.0009 | 0.0036 | 0.0001 | 0.0038 | 0.0011 | 0 | 0.0003 |
Golgi | 0.0011 | 0 | 0 | 0.0001 | 0.0011 | 0.0001 | 0.0041 | 0 | 0.0018 | 0.0011 | 0.0145 | 0.0002 | 0.0002 | 0 | 0.0006 | 0.0001 | 0 | 0.0002 |
Lipid Particles | 0.0033 | 0 | 0.0002 | 0.0006 | 0.0026 | 0.0001 | 0.0071 | 0 | 0.0029 | 0.0025 | 0.0085 | 0.0009 | 0.0004 | 0 | 0.0013 | 0.0006 | 0 | 0.0001 |
Mitochondria | 0.0007 | 0.0001 | 0.0002 | 0.0032 | 0.0015 | 0.0004 | 0.0195 | 0.0001 | 0.0105 | 0.0006 | 0.0229 | 0.001 | 0.0016 | 0.0016 | 0.0004 | 0.0021 | 0.0001 | 0.0001 |
None | 0.0532 | 0.0288 | 0.0356 | 0.043 | 0.0461 | 0.0112 | 0.0051 | 0.0001 | 0.0115 | 0.0003 | 0.0199 | 0.0048 | 0.0014 | 0.0134 | 0.0109 | 0.0014 | 0 | 0.0032 |
Nuclear Periphery | 0.0122 | 0.0052 | 0.0119 | 0.0056 | 0.0033 | 0.0066 | 0.0161 | 0.0042 | 0.0064 | 0.0018 | 0.0159 | 0.0035 | 0.0241 | 0.0047 | 0.0059 | 0.0066 | 0.0009 | 0.003 |
Nucleolus | 0.0195 | 0.0087 | 0.0208 | 0.0132 | 0.0198 | 0.0217 | 0.0098 | 0.0086 | 0.019 | 0.0379 | 0.0148 | 0.0163 | 0.007 | 0.0103 | 0.0046 | 0.015 | 0.017 | 0.0125 |
Nucleus | 0.8502 | 0.9516 | 0.88 | 0.8427 | 0.8843 | 0.9355 | 0.8794 | 0.9845 | 0.875 | 0.8349 | 0.8207 | 0.9575 | 0.9449 | 0.9605 | 0.9469 | 0.9638 | 0.9772 | 0.9521 |
Peroxisomes | 0.0015 | 0 | 0 | 0.0002 | 0.0029 | 0 | 0.0054 | 0 | 0.0054 | 0.0198 | 0.0034 | 0.0002 | 0.0001 | 0.0001 | 0.0007 | 0.0002 | 0 | 0 |
Punctate Nuclear | 0.0424 | 0.0045 | 0.0417 | 0.0385 | 0.0165 | 0.0144 | 0.0071 | 0.0012 | 0.007 | 0.0508 | 0.0041 | 0.0104 | 0.0074 | 0.0041 | 0.0085 | 0.0039 | 0.0034 | 0.0162 |
Vacuole | 0.0005 | 0.0001 | 0.0003 | 0.0013 | 0.0002 | 0.0002 | 0.0026 | 0.0004 | 0.0043 | 0.0042 | 0.0108 | 0.0005 | 0.0013 | 0.0002 | 0.0008 | 0.0009 | 0.0001 | 0.0001 |
Vacuole Periphery | 0.0004 | 0 | 0.0002 | 0.0003 | 0.0002 | 0.0002 | 0.0015 | 0.0001 | 0.001 | 0.0004 | 0.0088 | 0.0003 | 0.001 | 0.0001 | 0.0002 | 0.0006 | 0 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 14.8607 | 12.6203 | 12.3221 | 12.7836 | 8.6704 | 6.6836 | 16.4542 | 15.185 | 13.0136 | 14.6162 |
Translational Efficiency | 0.691 | 0.8303 | 0.83 | 0.7357 | 1.1406 | 1.5668 | 0.6654 | 0.8362 | 0.779 | 0.8494 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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