Standard name
Human Ortholog
Description Ser/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis; localization is cell cycle regulated; activated by Cdc15p during the exit from mitosis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; phosphorylates Chs2p to regulate primary septum formation and Hof1p to regulate cytokinesis; DBF2 has a paralog, DBF20, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.06 0.11 0.06 0.1 0.08 0.1 0.07 – 0 0 0 0 0 0 0 0.05 0.05 0 0 0.05 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0.06 0.08 0 0.05 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0.05 0 0 0 0 0 – 0 0 0 0 0 0 0.05 0.1 0.09 0.07 0 0 0 0 0 0
Cytoplasm 0.89 0.84 0.91 0.83 0.86 0.8 0.82 – 0.84 0.88 0.72 0.85 0.98 0.98 0.99 0.68 0.75 0.76 0.78 0.73 0.78 0.79 0.9 0.78
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.08 0.11 0.09 0 0 0 0 0 0.05
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0.05 – 0.12 0.09 0.35 0.12 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.07 0.05 0.06 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0.05 – 0 0 0 0 0 0 0 0.13 0.1 0.08 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.09 0.06 0.05 0 0 0.05 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 18 36 19 26 24 27 28 – 8 9 3 3 0 0 0 12 10 8 9 16 11 2 0 4
Bud 2 4 3 6 6 3 13 – 7 9 8 6 0 0 0 1 1 1 11 16 5 2 1 3
Bud Neck 0 0 0 3 3 1 5 – 5 4 1 2 0 0 0 0 0 0 19 26 10 4 0 1
Bud Site 1 1 0 3 4 12 9 – 2 0 3 1 0 0 0 3 1 0 – – – – – –
Cell Periphery 3 15 13 9 8 4 7 – 4 2 2 4 5 4 12 26 20 15 0 0 0 0 0 0
Cytoplasm 264 276 277 227 273 213 309 – 215 245 165 214 173 258 249 173 158 162 238 257 256 71 160 167
Endoplasmic Reticulum 11 10 6 9 4 11 15 – 1 2 6 6 0 3 1 8 4 10 3 1 4 0 0 1
Endosome 5 6 4 4 5 5 0 – 0 3 2 0 2 1 1 21 24 20 8 12 9 3 4 10
Golgi 1 3 1 0 0 0 0 – 0 1 0 0 0 0 0 5 5 1 2 3 1 0 0 2
Mitochondria 2 2 1 0 0 8 18 – 31 24 80 31 0 0 0 4 2 4 1 2 4 0 3 6
Nucleus 1 0 1 0 1 3 7 – 1 0 1 1 0 0 0 0 0 0 0 0 0 0 1 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 1 0 0 0 0 1 0 0 0 0 0 0 0 0
Nucleolus 1 0 1 1 3 2 2 – 1 0 1 1 0 0 0 18 11 12 0 0 0 0 0 0
Peroxisomes 1 3 0 1 3 1 3 – 3 2 0 0 0 0 0 1 2 0 0 1 0 0 0 3
SpindlePole 1 0 6 9 10 3 17 – 6 1 4 2 0 1 0 32 21 17 3 5 6 0 0 2
Vac/Vac Membrane 0 0 1 2 2 2 2 – 2 2 4 3 2 2 0 22 13 11 2 3 16 1 2 5
Unique Cell Count 298 328 306 272 319 267 376 256 278 229 251 177 264 251 255 211 214 304 351 330 90 178 214
Labelled Cell Count 311 356 333 300 346 295 435 286 304 281 274 182 269 263 327 272 261 304 351 330 90 178 214


Bud Neck, Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.7 9.9 8.7 8.1 8.0 6.8 6.4 – 5.3 5.1 4.4 4.6 8.3 8.3 7.5 12.0 12.5 11.2 7.4 8.2 8.7
Std Deviation (1e-4) 1.1 1.5 1.1 1.3 1.6 1.5 1.2 – 1.1 1.1 0.7 0.9 1.4 1.2 1.3 2.2 2.5 2.1 1.1 1.4 1.3
Intensity Change (Log2) – – – -0.11 -0.12 -0.35 -0.43 – -0.72 -0.77 -0.98 -0.93 -0.07 -0.08 -0.22 0.46 0.52 0.37 -0.22 -0.09 0.01


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 1.5 0.6 1.7 0.6 – -1.7 -1.7 0 0 -3.4 -4.1 -4.0 -0.8 -0.7 -1.2
Bud 0 0 0 2.1 – 0 1.9 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 3.7 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery -0.6 -1.2 -1.9 -1.8 – -1.9 -2.7 0 0 -0.8 -1.9 0.3 2.8 2.4 1.4
Cytoplasm -2.5 -1.9 -3.6 -3.1 – -2.3 -0.9 -5.6 -1.9 3.0 3.6 4.4 -6.7 -4.8 -4.6
Endoplasmic Reticulum 1.0 0 1.5 1.5 – 0 0 0 0 0 0 0 0.9 0 1.8
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 4.0 5.0 4.3
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 3.5 – 6.0 5.0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 4.4 0 3.8
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 1.0 0.9 0 1.8 – 0.3 0 0 0 0 0 0 5.0 4.0 3.3
Vacuole 0 0 0 0 – 0 0 0 0 0 0 0 4.9 4.0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.1656 4.8838 3.7823 3.7877 3.7594 4.9031 3.7934 4.1051 3.1573 2.8662 3.4102 4.5224 3.9275 4.7572 3.7547 3.644 3.8714 4.8237
Actin 0.0179 0.0063 0.0164 0.0048 0.0018 0.0069 0.0362 0.003 0.0089 0.1107 0.0054 0.0205 0.0865 0.0103 0.0226 0.0757 0.001 0.0406
Bud 0.0017 0.0017 0.003 0.0035 0.0029 0.0011 0.0016 0.0012 0.0009 0.0016 0.0019 0.0003 0.0087 0.0069 0.0036 0.0008 0.0001 0.0008
Bud Neck 0.0291 0.0017 0.0023 0.0007 0.0013 0.3533 0.034 0.0012 0.0019 0.0016 0.0045 0.459 0.0121 0.001 0.0151 0.0019 0.0795 0.421
Bud Periphery 0.002 0.001 0.003 0.0035 0.0024 0.0009 0.0026 0.0009 0.001 0.0019 0.0019 0.0005 0.0069 0.0021 0.0035 0.0008 0.0001 0.0007
Bud Site 0.0289 0.0565 0.0182 0.0027 0.0274 0.002 0.0514 0.0951 0.0123 0.0074 0.0184 0.0014 0.05 0.0717 0.0236 0.0009 0.0004 0.0021
Cell Periphery 0.0011 0.0006 0.0005 0.0002 0.0002 0.0002 0.0035 0.0021 0.0009 0.0003 0.0004 0.0008 0.0013 0.0007 0.0006 0.0001 0.0001 0.0005
Cytoplasm 0.6849 0.8631 0.7757 0.7832 0.6756 0.5276 0.6737 0.7833 0.8863 0.6035 0.7811 0.4126 0.6404 0.841 0.844 0.8072 0.8004 0.4538
Cytoplasmic Foci 0.0604 0.0243 0.0389 0.041 0.0878 0.0424 0.0301 0.0157 0.0194 0.041 0.0628 0.0719 0.0735 0.0316 0.0094 0.0553 0.0469 0.0481
Eisosomes 0.0001 0 0.0001 0 0 0 0.0005 0.0001 0 0.0006 0 0.0001 0.0005 0 0.0001 0.0002 0 0.0001
Endoplasmic Reticulum 0.004 0.0025 0.0029 0.0035 0.0023 0.0016 0.0217 0.0027 0.0064 0.0026 0.0067 0.0032 0.0108 0.0036 0.0155 0.0026 0.0035 0.0024
Endosome 0.0462 0.0074 0.0258 0.0476 0.087 0.0117 0.0261 0.0026 0.0135 0.0432 0.0418 0.0112 0.0234 0.0065 0.0157 0.0092 0.0269 0.005
Golgi 0.0179 0.0018 0.0139 0.0198 0.0216 0.0028 0.0084 0.0007 0.0047 0.0373 0.0078 0.003 0.0146 0.0011 0.0043 0.0039 0.0166 0.0083
Lipid Particles 0.0169 0.0002 0.0041 0.0082 0.0092 0.0015 0.0149 0.0003 0.0041 0.038 0.0143 0.0008 0.0145 0.0009 0.0035 0.0024 0.0098 0.0007
Mitochondria 0.0125 0.0005 0.0112 0.0195 0.039 0.0081 0.0066 0.0001 0.0008 0.0312 0.0029 0.0002 0.0119 0.0004 0.0052 0.0006 0.001 0.001
None 0.0377 0.023 0.0615 0.0325 0.0113 0.024 0.0472 0.0866 0.0271 0.0183 0.0213 0.0053 0.0125 0.0139 0.0172 0.0045 0.0024 0.0025
Nuclear Periphery 0.0037 0.0009 0.001 0.0064 0.0012 0.0003 0.0102 0.0004 0.0009 0.0018 0.0024 0.0003 0.0019 0.0006 0.0036 0.0005 0.0005 0.0005
Nucleolus 0.0007 0.0002 0.0003 0.0005 0.0007 0.0001 0.0006 0.0002 0.0002 0.0003 0.001 0.0001 0.0003 0.0001 0.0002 0.0002 0 0
Nucleus 0.0027 0.0018 0.0018 0.0033 0.0022 0.0008 0.0044 0.0009 0.0009 0.0007 0.0037 0.0006 0.0008 0.001 0.0011 0.0065 0.0006 0.0012
Peroxisomes 0.0173 0.0015 0.0145 0.0072 0.0041 0.0079 0.0174 0.0003 0.0047 0.0511 0.0124 0.0031 0.0201 0.0008 0.0077 0.0198 0.0082 0.0075
Punctate Nuclear 0.0048 0.0031 0.0016 0.0054 0.0041 0.0055 0.0042 0.0006 0.0011 0.0038 0.0025 0.0038 0.0074 0.0039 0.0008 0.0011 0.001 0.0028
Vacuole 0.0067 0.0017 0.0029 0.005 0.0124 0.0011 0.0036 0.002 0.0036 0.002 0.0059 0.0012 0.0013 0.0021 0.0023 0.0053 0.0008 0.0003
Vacuole Periphery 0.0029 0.0002 0.0006 0.0015 0.0055 0.0002 0.0011 0.0001 0.0003 0.0009 0.0009 0.0001 0.0006 0.0002 0.0004 0.0005 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 9.6283 21.2309 35.3406 36.8498 15.8125 12.1891 26.9782 40.7243 39.4495 18.974
Translational Efficiency 0.5526 0.8119 1.3445 1.0235 0.7474 0.8178 1.0752 1.0435 1.1261 1.0448

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✔
Gene Expression ✔
Translational Efficiency ✔
Standard name
Human Ortholog
Description Ser/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis; localization is cell cycle regulated; activated by Cdc15p during the exit from mitosis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; phosphorylates Chs2p to regulate primary septum formation and Hof1p to regulate cytokinesis; DBF2 has a paralog, DBF20, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (90%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Dbf2

Dbf2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Dbf2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available