Standard name | |
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Human Ortholog | |
Description | Splicing factor that modulates turnover of branched RNAs by Dbr1p; interacts with spliceosomal components and branched RNA splicing products; enhances Dbr1p debranching in vitro; conserved protein with domain organization identical from yeast to human; N-terminal homology to Dbr1p N-terminus, but Dbr1p catalytic residues not conserved; relocalizes to the cytosol in response to hypoxia |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 3 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 3 | 1 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 1 | 0 | 0 | 0 | 0 | 2 | 1 | 4 | 2 | 7 | 4 | 6 | 0 | 0 | 0 | 1 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 3 | 4 | 5 | 4 | 5 | 6 | 14 | 10 | 4 | 7 | 5 | 10 | 26 | 37 | 40 | 37 | 24 | 14 | 2 | 0 | 0 | 0 | 1 | 1 |
Endoplasmic Reticulum | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 0 | 0 | 0 | 0 | 2 | 1 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 1 | 1 | 0 | 0 | 0 | 1 | 2 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 4 | 0 | 0 | 1 | 1 | 0 | 0 | 1 |
Mitochondria | 36 | 12 | 34 | 7 | 23 | 51 | 143 | 67 | 104 | 157 | 108 | 116 | 4 | 4 | 5 | 12 | 5 | 3 | 3 | 2 | 2 | 0 | 3 | 2 |
Nucleus | 251 | 283 | 138 | 70 | 122 | 126 | 185 | 177 | 146 | 199 | 140 | 149 | 134 | 233 | 223 | 253 | 178 | 140 | 247 | 272 | 135 | 106 | 161 | 106 |
Nuclear Periphery | 4 | 1 | 0 | 0 | 0 | 3 | 6 | 7 | 5 | 5 | 4 | 4 | 0 | 0 | 2 | 2 | 4 | 2 | 0 | 0 | 0 | 0 | 0 | 3 |
Nucleolus | 1 | 2 | 1 | 0 | 1 | 2 | 9 | 7 | 10 | 20 | 18 | 14 | 0 | 3 | 2 | 4 | 1 | 4 | 0 | 0 | 1 | 1 | 7 | 8 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 1 | 0 | 0 | 0 | 0 | 3 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 0 | 1 | 0 | 0 | 0 | 2 | 0 |
Vac/Vac Membrane | 2 | 6 | 0 | 1 | 2 | 0 | 2 | 4 | 0 | 0 | 1 | 5 | 1 | 3 | 8 | 8 | 4 | 1 | 6 | 8 | 8 | 6 | 25 | 40 |
Unique Cell Count | 264 | 298 | 152 | 75 | 134 | 141 | 249 | 220 | 188 | 244 | 177 | 211 | 163 | 274 | 271 | 303 | 210 | 161 | 270 | 290 | 158 | 118 | 210 | 171 |
Labelled Cell Count | 299 | 310 | 178 | 82 | 153 | 191 | 364 | 277 | 275 | 395 | 280 | 305 | 165 | 282 | 283 | 322 | 225 | 167 | 270 | 290 | 158 | 118 | 210 | 171 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.3 | 5.9 | 5.1 | 5.2 | 5.0 | 4.4 | 3.6 | 4.4 | 4.0 | 3.8 | 3.7 | 4.0 | 6.5 | 6.3 | 6.4 | 7.6 | 9.2 | 9.6 | 6.4 | 6.0 | 6.9 |
Std Deviation (1e-4) | 0.8 | 1.0 | 1.2 | 1.3 | 1.4 | 1.6 | 1.2 | 1.5 | 1.0 | 0.9 | 1.1 | 1.8 | 1.9 | 1.6 | 1.6 | 1.8 | 2.1 | 2.0 | 2.3 | 1.6 | 1.6 |
Intensity Change (Log2) | – | – | – | 0.04 | -0.03 | -0.2 | -0.48 | -0.19 | -0.35 | -0.42 | -0.47 | -0.33 | 0.35 | 0.3 | 0.33 | 0.59 | 0.86 | 0.92 | 0.33 | 0.25 | 0.44 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 0.4 | 1.1 | 0.6 | 0 | 0 | 0 | 0.7 | 3.8 | 3.4 | 3.7 | 3.1 | 2.8 | 2.0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | -2.4 | -1.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -5.4 | -7.3 | -7.0 | -6.1 | -6.1 | -5.6 |
Nucleus | 0.7 | 0.1 | -0.4 | -4.0 | -2.7 | -3.2 | -2.5 | -2.9 | -4.7 | -2.2 | -1.7 | -2.4 | -2.1 | -1.7 | -1.1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.6326 | 4.2933 | 3.2316 | 2.9618 | 3.5045 | 3.198 | 3.0747 | 3.19 | 3.2566 | 3.0097 | 2.8933 | 3.2094 | 5.1601 | 4.5867 | 4.0116 | 3.5523 | 3.6893 | 4.2832 |
Actin | 0.2158 | 0 | 0.0006 | 0 | 0 | 0.0001 | 0.0171 | 0 | 0.0123 | 0 | 0.0017 | 0.002 | 0.0012 | 0.0001 | 0.0024 | 0 | 0 | 0.0007 |
Bud | 0.0012 | 0 | 0.0001 | 0 | 0 | 0.0002 | 0.0013 | 0 | 0.0001 | 0 | 0.0002 | 0.0001 | 0 | 0.0009 | 0.0004 | 0 | 0 | 0.0001 |
Bud Neck | 0.0005 | 0 | 0.0002 | 0.0001 | 0.0001 | 0.0017 | 0.0007 | 0.0001 | 0.0006 | 0 | 0.0004 | 0.0011 | 0.0001 | 0.0002 | 0.0004 | 0.0002 | 0.0001 | 0.001 |
Bud Periphery | 0.0025 | 0 | 0.0002 | 0 | 0 | 0.0007 | 0.0025 | 0 | 0.0003 | 0 | 0.0003 | 0.0002 | 0 | 0.0015 | 0.0006 | 0 | 0 | 0.0003 |
Bud Site | 0.0043 | 0.0001 | 0.0001 | 0 | 0 | 0.0015 | 0.0009 | 0 | 0.001 | 0 | 0.003 | 0.0003 | 0.0001 | 0.0013 | 0.0011 | 0.0001 | 0 | 0.0002 |
Cell Periphery | 0.0011 | 0 | 0.0001 | 0 | 0 | 0.0002 | 0.0003 | 0 | 0.0002 | 0 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0001 | 0 | 0 | 0.0001 |
Cytoplasm | 0.0038 | 0.0022 | 0.0001 | 0.0005 | 0 | 0.0003 | 0.0029 | 0.0002 | 0.0018 | 0.0001 | 0.0021 | 0.0003 | 0.0087 | 0.0535 | 0.0035 | 0.0001 | 0 | 0.0005 |
Cytoplasmic Foci | 0.0034 | 0 | 0.0003 | 0 | 0 | 0.0007 | 0.0049 | 0 | 0.0023 | 0 | 0.0037 | 0.0029 | 0.0182 | 0.0014 | 0.0113 | 0 | 0 | 0.0057 |
Eisosomes | 0.006 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0224 | 0.0001 | 0 | 0.0003 | 0 | 0.0001 | 0.003 | 0 | 0.0024 | 0 | 0.0009 | 0.0002 | 0.0002 | 0.0004 | 0.0023 | 0 | 0 | 0.0001 |
Endosome | 0.0158 | 0.0001 | 0.0017 | 0.0003 | 0 | 0.0034 | 0.016 | 0 | 0.0058 | 0 | 0.0104 | 0.0038 | 0.0023 | 0.0007 | 0.0277 | 0 | 0 | 0.001 |
Golgi | 0.0158 | 0 | 0.0013 | 0 | 0 | 0.0003 | 0.0022 | 0 | 0.0022 | 0 | 0.0026 | 0.0013 | 0.0003 | 0.0001 | 0.0203 | 0 | 0 | 0.0006 |
Lipid Particles | 0.0048 | 0 | 0.001 | 0 | 0 | 0.0006 | 0.0056 | 0 | 0.0086 | 0 | 0.0043 | 0.0104 | 0.0009 | 0.0007 | 0.0301 | 0 | 0 | 0.0091 |
Mitochondria | 0.0094 | 0.0004 | 0.0249 | 0.0005 | 0.0001 | 0.0009 | 0.0071 | 0.0001 | 0.0029 | 0.0004 | 0.0201 | 0.0027 | 0.0001 | 0.0029 | 0.013 | 0.0003 | 0.0002 | 0.0008 |
None | 0.0132 | 0.0001 | 0 | 0.0007 | 0 | 0.0001 | 0.0062 | 0 | 0.0014 | 0 | 0.0018 | 0.0018 | 0.0174 | 0.011 | 0.0003 | 0 | 0 | 0.0003 |
Nuclear Periphery | 0.0076 | 0.0117 | 0.0005 | 0.0584 | 0.0002 | 0.0007 | 0.0177 | 0.0045 | 0.0086 | 0.0032 | 0.0048 | 0.0048 | 0.0026 | 0.0021 | 0.01 | 0.001 | 0.0003 | 0.0022 |
Nucleolus | 0.0028 | 0.0047 | 0.0028 | 0.0057 | 0.0079 | 0.0318 | 0.0069 | 0.0082 | 0.0077 | 0.0034 | 0.0201 | 0.0168 | 0.0021 | 0.014 | 0.0034 | 0.0071 | 0.0133 | 0.0188 |
Nucleus | 0.6648 | 0.9778 | 0.9645 | 0.93 | 0.9915 | 0.9473 | 0.8896 | 0.9862 | 0.9348 | 0.9924 | 0.9106 | 0.9419 | 0.8006 | 0.9017 | 0.8611 | 0.9908 | 0.9857 | 0.9497 |
Peroxisomes | 0.0015 | 0 | 0.0005 | 0 | 0 | 0.0001 | 0.0022 | 0 | 0.0017 | 0 | 0.0073 | 0.003 | 0.0005 | 0.0002 | 0.004 | 0 | 0 | 0.0017 |
Punctate Nuclear | 0.001 | 0.0002 | 0 | 0.0007 | 0.0001 | 0.0004 | 0.0048 | 0.0005 | 0.003 | 0.0002 | 0.004 | 0.0046 | 0.1444 | 0.0038 | 0.0028 | 0.0001 | 0.0001 | 0.0066 |
Vacuole | 0.001 | 0.0015 | 0.0006 | 0.0006 | 0 | 0.0081 | 0.005 | 0.0001 | 0.0015 | 0 | 0.0012 | 0.0014 | 0.0001 | 0.0021 | 0.003 | 0.0001 | 0 | 0.0004 |
Vacuole Periphery | 0.0013 | 0.0011 | 0.0003 | 0.002 | 0 | 0.0006 | 0.0027 | 0.0001 | 0.0006 | 0.0001 | 0.0004 | 0.0004 | 0 | 0.0012 | 0.0021 | 0.0001 | 0 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 18.4625 | 21.3585 | 16.8095 | 19.3839 | 20.1825 | 22.4322 | 19.4688 | 22.0842 | 17.5222 | 20.247 |
Translational Efficiency | 1.2992 | 1.2039 | 1.2426 | 0.9832 | 0.9545 | 0.8447 | 1.1677 | 0.9306 | 0.9586 | 0.9661 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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