Standard name
Human Ortholog
Description B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the transition from G2 to M phase; accumulates during G2 and M, then targeted via a destruction box motif for ubiquitin-mediated degradation by the proteasome; CLB1 has a paralog, CLB2, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm – 0.91 0.95 0.85 0.84 0.76 0.7 0.66 0.58 0.63 0.45 0.46 0.7 0.81 0.87 0.93 0.89 0.93 0.39 0.63 0.63 0.89 0.84 0.77
Endoplasmic Reticulum – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.13 0.09 0.24 0 0 0 0 0
Endosome – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria – 0.05 0 0.14 0.07 0.38 0.38 0.37 0.6 0.54 0.66 0.71 0 0 0 0 0 0 0.12 0.1 0.19 0 0 0
Nucleus – 0 0 0 0 0 0 0 0 0 0 0 0.13 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane – 0.06 0 0.05 0.09 0 0 0 0 0 0 0 0.18 0.17 0.14 0 0.09 0.05 0.05 0.07 0 0 0 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 3 4 5 1 3 5
Bud – 0 2 2 0 1 1 6 2 5 7 4 0 0 0 0 0 0 0 3 1 0 6 5
Bud Neck – 0 1 0 1 1 0 1 2 3 0 0 0 0 0 0 1 0 0 0 1 0 1 4
Bud Site – 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 – – – – – –
Cell Periphery – 0 0 1 2 1 3 1 2 0 4 3 0 0 0 4 7 4 0 1 1 0 0 1
Cytoplasm – 158 195 138 202 233 366 314 173 219 156 198 124 260 306 253 214 179 37 123 136 97 251 180
Endoplasmic Reticulum – 3 1 0 0 4 1 3 2 2 3 6 1 0 1 20 31 17 23 1 1 0 1 1
Endosome – 0 3 2 6 2 8 12 1 2 1 0 5 11 9 6 3 1 3 4 6 2 3 1
Golgi – 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 2 2 0 1 2
Mitochondria – 8 8 22 17 116 199 174 179 190 232 305 0 0 0 6 2 0 11 20 40 4 13 7
Nucleus – 0 0 2 2 0 8 16 8 5 14 10 23 9 2 3 3 2 0 8 0 0 0 0
Nuclear Periphery – 0 0 0 0 0 2 1 0 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0
Nucleolus – 0 0 1 2 0 0 0 0 1 0 0 2 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes – 0 0 1 0 0 3 2 0 0 3 1 0 0 0 1 0 0 0 0 0 0 1 0
SpindlePole – 1 0 0 2 4 6 8 1 6 5 4 0 0 0 1 0 1 0 4 3 0 5 2
Vac/Vac Membrane – 10 2 8 21 5 16 10 7 10 13 8 31 53 49 10 22 10 5 13 9 0 4 13
Unique Cell Count 174 205 162 241 307 522 475 298 349 349 432 176 320 351 273 241 192 97 195 217 110 300 233
Labelled Cell Count 181 212 177 255 368 613 548 377 444 440 540 186 333 367 305 284 215 97 195 217 110 300 233


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – 4.1 3.8 3.7 4.0 3.2 2.9 3.2 3.0 2.8 2.9 2.9 4.9 4.4 4.4 4.9 5.7 5.5 4.2 4.2 5.1
Std Deviation (1e-4) – 1.1 1.1 1.7 1.9 1.0 1.1 1.1 1.1 1.2 0.6 1.6 1.6 1.1 1.2 1.4 1.2 1.5 1.7 1.6 2.0
Intensity Change (Log2) – – – -0.05 0.06 -0.24 -0.39 -0.25 -0.35 -0.46 -0.4 -0.39 0.34 0.19 0.21 0.35 0.56 0.52 0.11 0.11 0.42


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 1.9 3.8 2.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -1.144 -0.4624 -0.5799 -0.3061 -0.5342 -0.8507 2.005 1.6163 2.0598 2.1598 2.1241 1.4704 2.9538 2.9836 2.9849 3.3805 3.0031 2.4226
Actin 0.0243 0.0035 0.0018 0.0298 0.003 0.0017 0.0301 0.0005 0.0506 0.0202 0.0187 0.0021 0.0597 0.0015 0.0088 0.0122 0.0064 0.0029
Bud 0.0023 0.0011 0.0002 0.0002 0.0002 0.0001 0.0014 0.0013 0.0058 0.0098 0.0019 0.0009 0.0028 0.0047 0.0011 0.0142 0.0098 0.0017
Bud Neck 0.0061 0.0003 0.0003 0.0011 0.0012 0.0007 0.0028 0.0001 0.0017 0.0011 0.0116 0.0001 0.0035 0.0005 0.0025 0.0007 0.0006 0.0004
Bud Periphery 0.0049 0.0013 0.0008 0.0002 0.0001 0.0001 0.0033 0.0049 0.0039 0.0248 0.0025 0.0033 0.0029 0.0046 0.0013 0.0082 0.0064 0.0042
Bud Site 0.0058 0.0036 0.0004 0.0008 0.0003 0.0001 0.0121 0.0031 0.0098 0.0152 0.0346 0.0001 0.0057 0.0064 0.0151 0.0022 0.0009 0.0002
Cell Periphery 0.0003 0.0001 0.0002 0.0002 0.0001 0 0.0008 0.0003 0.0006 0.0009 0.0006 0.0001 0.0005 0.0008 0.0011 0.0005 0.0003 0.0001
Cytoplasm 0.0369 0.1295 0.1007 0.2393 0.2248 0.0834 0.0631 0.0761 0.0836 0.1821 0.2139 0.0514 0.0514 0.09 0.1097 0.1925 0.1305 0.076
Cytoplasmic Foci 0.0138 0.0074 0.0078 0.0026 0.0231 0.011 0.0615 0.0046 0.0265 0.0053 0.0379 0.0054 0.0162 0.0101 0.0196 0.0247 0.0206 0.011
Eisosomes 0.0008 0.0002 0.0004 0.0002 0.0001 0.0002 0.0009 0.0003 0.0005 0.0005 0.0002 0.0001 0.0006 0.0015 0.0002 0.0003 0.0002 0.0001
Endoplasmic Reticulum 0.0037 0.0024 0.0032 0.0036 0.0041 0.0005 0.0063 0.0009 0.0034 0.0016 0.0038 0.0005 0.006 0.0012 0.0031 0.0026 0.0102 0.0007
Endosome 0.014 0.0016 0.0044 0.0027 0.0112 0.0013 0.0252 0.0012 0.0107 0.0052 0.0146 0.001 0.01 0.0046 0.0076 0.0084 0.0102 0.0038
Golgi 0.004 0.0004 0.0004 0.0009 0.0011 0.0006 0.0167 0.0001 0.0098 0.0034 0.008 0.0018 0.0054 0.0035 0.002 0.0017 0.0008 0.0029
Lipid Particles 0.0075 0.0006 0.0021 0.0005 0.0018 0.0043 0.0164 0.0007 0.0067 0.003 0.0042 0.0017 0.0096 0.0012 0.0034 0.0045 0.003 0.007
Mitochondria 0.0071 0.0007 0.0132 0.0007 0.001 0.0039 0.0158 0.0008 0.0063 0.0326 0.005 0.0088 0.0051 0.0011 0.0016 0.0034 0.0024 0.0012
None 0.822 0.7064 0.7164 0.4476 0.6614 0.8624 0.6961 0.8647 0.6466 0.4487 0.5064 0.9094 0.772 0.8385 0.6439 0.5137 0.7303 0.8635
Nuclear Periphery 0.0107 0.0113 0.0086 0.0121 0.0192 0.0005 0.0034 0.0011 0.0078 0.0033 0.031 0.0004 0.0118 0.0011 0.0096 0.0059 0.0145 0.0011
Nucleolus 0.0016 0.0016 0.0022 0.0007 0.0008 0.0006 0.0055 0.0005 0.0024 0.0019 0.0011 0.0006 0.001 0.0007 0.0012 0.0042 0.0013 0.0003
Nucleus 0.0112 0.1112 0.1147 0.2458 0.0224 0.0128 0.0039 0.0353 0.0856 0.2258 0.0425 0.0102 0.0058 0.0131 0.1499 0.1609 0.0421 0.0153
Peroxisomes 0.0131 0.0043 0.0032 0.001 0.0012 0.0076 0.0215 0.0004 0.021 0.0029 0.042 0.0011 0.0169 0.0023 0.0053 0.0024 0.0006 0.0036
Punctate Nuclear 0.0054 0.01 0.0169 0.0076 0.021 0.0079 0.0084 0.0018 0.011 0.0067 0.0163 0.0003 0.0097 0.0013 0.0104 0.0333 0.0037 0.0033
Vacuole 0.0029 0.0022 0.0017 0.0018 0.0015 0.0004 0.0039 0.001 0.0048 0.0039 0.0019 0.0004 0.002 0.0107 0.0019 0.0025 0.0038 0.0005
Vacuole Periphery 0.0015 0.0003 0.0006 0.0004 0.0006 0.0001 0.001 0.0001 0.0007 0.0013 0.0014 0.0002 0.0012 0.0005 0.0007 0.001 0.0014 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 1.5585 25.0774 21.7099 19.1822 10.6084 1.5576 33.6315 35.6529 27.0473 12.4699
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency –
Standard name
Human Ortholog
Description B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the transition from G2 to M phase; accumulates during G2 and M, then targeted via a destruction box motif for ubiquitin-mediated degradation by the proteasome; CLB1 has a paralog, CLB2, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (40%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Clb1

Clb1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Clb1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available