ORF | |
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Human Ortholog | |
Description | Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Bud | 0 | 0 | 1 | 0 | 4 | 7 | 10 | 13 | 9 | 7 | 18 | 17 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 1 | 0 | 6 | 2 | 12 | 1 | 9 | 6 | 15 | 9 | 11 | 9 | 9 | 7 | 5 | 14 | 7 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 88 | 74 | 119 | 266 | 333 | 446 | 409 | 555 | 400 | 542 | 275 | 310 | 211 | 345 | 334 | 256 | 255 | 149 | 91 | 88 | 117 | 98 | 257 | 364 |
Endoplasmic Reticulum | 1 | 1 | 1 | 1 | 1 | 0 | 4 | 2 | 0 | 2 | 0 | 0 | 2 | 8 | 5 | 14 | 7 | 4 | 0 | 0 | 1 | 0 | 6 | 9 |
Endosome | 0 | 0 | 0 | 1 | 0 | 3 | 1 | 7 | 3 | 8 | 0 | 2 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 0 |
Golgi | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 1 | 0 | 1 | 0 | 0 |
Mitochondria | 1 | 2 | 3 | 11 | 43 | 177 | 222 | 287 | 255 | 279 | 217 | 222 | 0 | 0 | 0 | 4 | 6 | 0 | 1 | 1 | 0 | 2 | 2 | 7 |
Nucleus | 0 | 3 | 1 | 1 | 1 | 2 | 3 | 10 | 5 | 3 | 5 | 2 | 2 | 0 | 0 | 3 | 5 | 2 | 0 | 0 | 0 | 0 | 0 | 2 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 1 | 4 | 4 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 1 | 0 | 3 | 2 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Vac/Vac Membrane | 0 | 0 | 0 | 2 | 1 | 4 | 7 | 20 | 14 | 20 | 10 | 6 | 0 | 0 | 0 | 1 | 2 | 12 | 0 | 2 | 0 | 6 | 15 | 13 |
Unique Cell Count | 89 | 79 | 121 | 273 | 348 | 529 | 525 | 739 | 542 | 720 | 396 | 435 | 212 | 352 | 339 | 263 | 265 | 161 | 96 | 98 | 124 | 117 | 291 | 411 |
Labelled Cell Count | 92 | 81 | 125 | 288 | 386 | 652 | 664 | 910 | 693 | 881 | 540 | 571 | 227 | 365 | 346 | 284 | 289 | 176 | 96 | 98 | 124 | 117 | 291 | 411 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.3 | 7.2 | 6.1 | 6.4 | 5.5 | 4.8 | 4.3 | 4.5 | 4.4 | 4.3 | 4.3 | 4.1 | 7.2 | 7.5 | 7.6 | 8.2 | 8.4 | 8.5 | 8.2 | 8.3 | 8.2 |
Std Deviation (1e-4) | 0.9 | 1.0 | 0.7 | 1.5 | 1.2 | 1.1 | 1.4 | 1.0 | 1.3 | 1.1 | 1.2 | 1.0 | 0.8 | 1.0 | 1.0 | 1.3 | 1.2 | 1.6 | 1.7 | 1.4 | 1.4 |
Intensity Change (Log2) | – | – | – | 0.07 | -0.14 | -0.34 | -0.49 | -0.43 | -0.47 | -0.5 | -0.51 | -0.58 | 0.24 | 0.31 | 0.33 | 0.44 | 0.47 | 0.48 | 0.43 | 0.45 | 0.44 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.6 | -1.3 | -4.1 | -5.2 | -5.8 | -5.9 | -5.7 | -6.5 | -6.3 | 1.1 | -0.2 | 0.1 | -0.6 | -1.1 | -2.2 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 3.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.1 |
R1 | R2 | R3 | ||||||||||||||||
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G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Actin | 0.0006 | 0.0017 | 0.0004 | 0.0013 | 0 | 0.0002 | 0.0004 | 0.0003 | 0.0006 | 0.0014 | 0.0011 | 0.0003 | – | – | – | – | – | – |
Bud | 0.0004 | 0.0006 | 0.001 | 0.0009 | 0.0003 | 0.0002 | 0.0002 | 0.0004 | 0.0004 | 0.0007 | 0.0002 | 0.0002 | – | – | – | – | – | – |
Bud Neck | 0.0017 | 0.0004 | 0.0008 | 0.0011 | 0.0004 | 0.0008 | 0.0002 | 0.0001 | 0.0002 | 0.0003 | 0.0003 | 0.0005 | – | – | – | – | – | – |
Bud Periphery | 0.0004 | 0.0003 | 0.0009 | 0.0005 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0006 | 0.0002 | 0.0001 | – | – | – | – | – | – |
Bud Site | 0.0044 | 0.0031 | 0.0061 | 0.0015 | 0.0008 | 0.0001 | 0.0016 | 0.0022 | 0.0011 | 0.0005 | 0.0002 | 0 | – | – | – | – | – | – |
Cell Periphery | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0 | 0 | 0.0003 | 0.0003 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | – | – | – | – | – | – |
Cytoplasm | 0.8687 | 0.9208 | 0.8914 | 0.8453 | 0.8869 | 0.8913 | 0.8529 | 0.9176 | 0.8458 | 0.7982 | 0.8741 | 0.9297 | – | – | – | – | – | – |
Cytoplasmic Foci | 0.0153 | 0.0193 | 0.0201 | 0.0282 | 0.0168 | 0.0183 | 0.0206 | 0.0158 | 0.0167 | 0.0209 | 0.0139 | 0.0084 | – | – | – | – | – | – |
Eisosomes | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Endoplasmic Reticulum | 0.0089 | 0.0022 | 0.0033 | 0.0042 | 0.0019 | 0.0037 | 0.005 | 0.0014 | 0.0024 | 0.0047 | 0.0055 | 0.0025 | – | – | – | – | – | – |
Endosome | 0.0139 | 0.0139 | 0.0212 | 0.0252 | 0.0144 | 0.0201 | 0.0138 | 0.014 | 0.0283 | 0.057 | 0.0256 | 0.0078 | – | – | – | – | – | – |
Golgi | 0.0015 | 0.0013 | 0.001 | 0.0016 | 0.0004 | 0.0009 | 0.0012 | 0.0014 | 0.0014 | 0.0027 | 0.0012 | 0.0006 | – | – | – | – | – | – |
Lipid Particles | 0.0004 | 0.0001 | 0.0003 | 0.0005 | 0 | 0.0001 | 0.001 | 0.0001 | 0.0002 | 0.0007 | 0.0002 | 0.0001 | – | – | – | – | – | – |
Mitochondria | 0.0025 | 0.0006 | 0.0006 | 0.0009 | 0.0004 | 0.0011 | 0.0011 | 0.0002 | 0.0006 | 0.0009 | 0.0003 | 0.0003 | – | – | – | – | – | – |
None | 0.0704 | 0.0283 | 0.0379 | 0.0729 | 0.0698 | 0.0579 | 0.0901 | 0.0422 | 0.0866 | 0.1008 | 0.0708 | 0.0471 | – | – | – | – | – | – |
Nuclear Periphery | 0.0012 | 0.0004 | 0.001 | 0.0018 | 0.0005 | 0.0003 | 0.0009 | 0.0004 | 0.0006 | 0.0009 | 0.0006 | 0.0002 | – | – | – | – | – | – |
Nucleolus | 0.0001 | 0.0001 | 0.0004 | 0.0001 | 0 | 0 | 0.002 | 0 | 0.0044 | 0.0001 | 0 | 0 | – | – | – | – | – | – |
Nucleus | 0.0029 | 0.0018 | 0.0032 | 0.0029 | 0.0021 | 0.001 | 0.0023 | 0.0005 | 0.0009 | 0.0008 | 0.0006 | 0.0005 | – | – | – | – | – | – |
Peroxisomes | 0.0005 | 0.0005 | 0.0003 | 0.0005 | 0.0001 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | – | – | – | – | – | – |
Punctate Nuclear | 0.0004 | 0.0006 | 0.0014 | 0.0024 | 0.0003 | 0.0002 | 0.0013 | 0.0001 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | – | – | – | – | – | – |
Vacuole | 0.005 | 0.0035 | 0.0076 | 0.0067 | 0.0042 | 0.0027 | 0.0038 | 0.0024 | 0.0077 | 0.0068 | 0.004 | 0.0013 | – | – | – | – | – | – |
Vacuole Periphery | 0.0005 | 0.0004 | 0.0008 | 0.0014 | 0.0005 | 0.0006 | 0.0004 | 0.0004 | 0.0011 | 0.0014 | 0.0007 | 0.0003 | – | – | – | – | – | – |
R1 | R2 | |||||||||
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G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 26.2165 | 15.8537 | 24.5512 | 28.2638 | 27.9924 | 21.5781 | 23.1733 | 23.7546 | 26.3955 | 30.9505 |
Translational Efficiency | 0.6209 | 0.9269 | 0.6678 | 0.5709 | 0.5293 | 0.7374 | 0.7406 | 0.643 | 0.6951 | 0.536 |
Dataset | Hit |
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Protein Concentration | – |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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