ORF
Human Ortholog
Description Protein that may be involved in pH regulation; probable ortholog of A. nidulans PalC, which is involved in pH regulation and binds to the ESCRT-III complex; null mutant does not properly process Rim101p and has decreased resistance to rapamycin; GFP-fusion protein is cytoplasmic; relative distribution to cytoplasm increases upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.08 0 0 0 0 0 0 0 0 0 0 0.07 0.08 0.06 – – – 0.29 0.27 0.39 0.21 0.28 0.27
Bud 0 0.08 0.07 0.06 0.06 0.05 0.08 0.07 0.06 0.06 0.06 0.09 0 0 0 – – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0.06 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 0 0.06 0.09 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 0.89 0.88 0.74 0.82 0.83 0.77 0.68 0.64 0.57 0.6 0.52 0.47 0.89 0.91 0.91 – – – 0.51 0.51 0.35 0.58 0.51 0.57
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Endosome 0 0 0.08 0 0 0 0 0.07 0 0 0 0 0 0 0 – – – 0.07 0.06 0.05 0.07 0.05 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Mitochondria 0.13 0 0 0.16 0.06 0.27 0.32 0.33 0.45 0.48 0.59 0.54 0 0 0 – – – 0 0.06 0.1 0 0.05 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0.07 0.08 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.07 0.07 0.09 0.08 0.05 0 0 0 – – – 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 4 10 8 2 7 4 4 2 3 0 0 0 11 19 20 – – – 33 34 87 27 70 77
Bud 2 10 14 7 12 11 28 21 18 17 10 15 5 10 5 – – – 0 0 0 0 0 3
Bud Neck 0 0 1 0 1 0 1 1 0 0 0 0 0 2 2 – – – 1 8 5 3 10 11
Bud Site 1 0 0 0 1 3 2 3 0 5 1 2 0 0 0 – – – – – – – – –
Cell Periphery 0 1 12 10 6 3 11 7 6 8 4 4 3 8 9 – – – 1 0 3 2 3 3
Cytoplasm 87 107 151 94 172 173 233 205 160 168 89 80 149 215 298 – – – 58 66 78 78 127 161
Endoplasmic Reticulum 1 5 1 1 4 0 3 2 2 1 1 1 7 4 2 – – – 2 0 2 0 0 0
Endosome 0 0 16 4 6 5 2 21 2 1 4 2 1 1 1 – – – 7 7 11 9 11 9
Golgi 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 – – – 0 0 1 0 1 0
Mitochondria 13 2 7 18 13 61 110 104 127 134 100 92 0 0 0 – – – 4 8 21 5 13 9
Nucleus 0 0 0 1 0 0 1 1 3 4 2 1 0 0 1 – – – 0 0 0 0 0 0
Nuclear Periphery 1 0 0 0 0 0 0 0 0 1 0 1 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0 0 2 0 1 0 1 3 2 0 1 1 0 0 0 – – – 0 0 1 0 0 0
Peroxisomes 0 0 12 3 2 1 0 6 1 0 0 0 0 0 0 – – – 1 0 4 1 1 1
SpindlePole 1 0 15 9 8 5 6 9 4 1 2 3 5 3 8 – – – 1 0 4 0 3 1
Vac/Vac Membrane 0 0 7 1 4 3 10 22 20 24 13 8 0 0 0 – – – 0 0 0 3 3 2
Unique Cell Count 98 122 204 114 207 226 342 320 282 281 170 169 167 237 326 113 131 227 135 251 286
Labelled Cell Count 110 135 246 150 238 269 412 407 348 364 227 210 181 262 346 113 131 227 135 251 286


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.6 5.8 6.3 6.2 6.5 5.4 5.3 5.3 4.8 4.9 4.3 4.6 6.3 6.5 6.3 – – – 5.7 6.6 7.0
Std Deviation (1e-4) 0.5 0.9 2.6 3.1 3.8 2.4 2.1 1.8 1.1 2.1 1.3 1.7 1.0 1.4 1.1 – – – 0.9 1.4 1.6
Intensity Change (Log2) – – – -0.03 0.04 -0.24 -0.26 -0.26 -0.39 -0.36 -0.57 -0.45 -0.01 0.03 -0.0 – – – -0.15 0.05 0.14


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 -0.3 -1.4 0 0 0 0 0 0 1.2 1.8 1.1 – – –
Bud -0.2 -0.4 -0.9 0.6 -0.1 0 -0.4 0 0 -1.7 -1.2 -3.2 – – –
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Cell Periphery 1.0 -1.5 -2.6 -1.5 -2.2 0 -1.7 0 0 -2.0 -1.3 -1.8 – – –
Cytoplasm 1.7 2.2 0.6 -1.5 -2.4 -3.9 -3.3 -4.4 -5.3 3.7 4.7 5.4 – – –
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Endosome -1.5 -2.2 -2.7 -4.6 -0.6 0 -4.4 0 0 -3.3 -4.0 -4.8 – – –
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Mitochondria 3.9 1.3 6.7 7.9 7.9 0 10.6 0 0 0 0 0 – – –
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Peroxisomes -1.3 -2.7 -3.3 0 -2.5 0 -4.1 0 0 -3.2 -3.8 0 – – –
SpindlePole 0.2 -1.5 -2.5 -3.3 -2.4 0 -4.3 0 0 -1.8 -3.2 -2.7 – – –
Vacuole 0 -0.9 0 -0.3 1.7 1.7 2.3 1.8 0.6 0 0 0 – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.416 1.7199 1.5974 1.2043 1.3516 1.6842 0.8388 1.2548 1.147 1.064 0.8837 0.9534 0.062 0.4629 -0.0727 0.1903 0.032 0.1783
Actin 0.0574 0.0343 0.0444 0.0979 0.0719 0.0257 0.0582 0.0191 0.0625 0.0628 0.0357 0.0384 0.0451 0.0413 0.0932 0.0196 0.0043 0.0254
Bud 0.0008 0.0006 0.0005 0.0013 0.0005 0.0003 0.0012 0.0017 0.0012 0.0016 0.0032 0.0018 0.0003 0.0005 0.002 0.0005 0.0001 0.0003
Bud Neck 0.0046 0.0022 0.0051 0.0029 0.013 0.0078 0.0041 0.0015 0.0029 0.0018 0.0059 0.01 0.0023 0.0011 0.0039 0.0012 0.0004 0.0195
Bud Periphery 0.0009 0.0009 0.0004 0.0022 0.001 0.0004 0.0016 0.001 0.0012 0.0014 0.0049 0.0026 0.0003 0.0003 0.0016 0.0006 0 0.0002
Bud Site 0.0074 0.0085 0.0015 0.001 0.0005 0.0002 0.0047 0.02 0.0023 0.0037 0.0128 0.0012 0.0024 0.0143 0.0144 0.0007 0.0001 0.0021
Cell Periphery 0.0008 0.0005 0.0005 0.0003 0.0002 0.0002 0.0005 0.0004 0.0003 0.0001 0.0004 0.0001 0.0003 0.0007 0.0004 0.0002 0.0001 0.0002
Cytoplasm 0.4353 0.496 0.4415 0.5856 0.4289 0.5665 0.5767 0.7521 0.6268 0.6982 0.5972 0.7868 0.4913 0.7041 0.5348 0.6523 0.5711 0.6835
Cytoplasmic Foci 0.128 0.0839 0.1123 0.0722 0.1404 0.0635 0.0896 0.039 0.0751 0.0742 0.0482 0.0578 0.1043 0.0309 0.0972 0.0652 0.022 0.0733
Eisosomes 0.0004 0.0003 0.0003 0.0002 0.0003 0.0002 0.0004 0.0001 0.0002 0.0001 0.0003 0.0001 0.0002 0.0002 0.0002 0.0002 0.0001 0.0001
Endoplasmic Reticulum 0.0037 0.0021 0.0031 0.0059 0.0027 0.0029 0.0103 0.0051 0.0054 0.0043 0.0063 0.006 0.0074 0.0044 0.0039 0.0071 0.0059 0.0074
Endosome 0.0077 0.0063 0.0074 0.0128 0.0063 0.0041 0.0245 0.0057 0.0231 0.0255 0.0323 0.013 0.0173 0.0021 0.0047 0.0221 0.0079 0.008
Golgi 0.0061 0.0023 0.0037 0.0039 0.0068 0.0013 0.014 0.0029 0.0085 0.0121 0.0214 0.0024 0.0047 0.0015 0.0035 0.0029 0.001 0.0024
Lipid Particles 0.0055 0.0005 0.0019 0.001 0.0013 0.0005 0.0136 0.0006 0.0029 0.0205 0.0423 0.0011 0.0036 0.0006 0.0017 0.0012 0.0005 0.0019
Mitochondria 0.0015 0.0006 0.0009 0.0033 0.0013 0.0007 0.0067 0.0004 0.0144 0.0036 0.0711 0.0011 0.0013 0.0003 0.0008 0.0008 0.0002 0.0005
None 0.3011 0.3441 0.3541 0.1904 0.286 0.315 0.1479 0.1433 0.1366 0.0428 0.0746 0.0566 0.2743 0.1911 0.2115 0.2131 0.3824 0.1667
Nuclear Periphery 0.0011 0.0004 0.0005 0.0012 0.0003 0.0004 0.0062 0.0009 0.0037 0.0025 0.0049 0.0041 0.0122 0.0006 0.0007 0.0019 0.0008 0.0007
Nucleolus 0.0004 0.0002 0.0001 0.0003 0.0001 0.0001 0.0023 0.0001 0.0004 0.0002 0.0011 0.0004 0.0005 0.0001 0.0001 0.0001 0 0.0001
Nucleus 0.0038 0.0013 0.0008 0.0038 0.0004 0.0009 0.0029 0.0012 0.0021 0.0018 0.0062 0.0023 0.0058 0.0009 0.0008 0.0017 0.0009 0.0011
Peroxisomes 0.0295 0.0121 0.0185 0.0081 0.036 0.0077 0.0221 0.0026 0.0229 0.0347 0.0212 0.0091 0.0176 0.0031 0.0206 0.0043 0.0006 0.0043
Punctate Nuclear 0.003 0.0021 0.0018 0.0034 0.0017 0.0011 0.0086 0.0012 0.0055 0.005 0.0058 0.0037 0.0054 0.0013 0.0031 0.0025 0.0009 0.0018
Vacuole 0.0008 0.0007 0.0005 0.0019 0.0003 0.0004 0.0025 0.0009 0.0013 0.0023 0.0024 0.001 0.002 0.0005 0.0005 0.0013 0.0006 0.0005
Vacuole Periphery 0.0002 0.0001 0.0001 0.0004 0.0001 0.0001 0.0012 0.0001 0.0006 0.0007 0.0017 0.0003 0.0013 0.0001 0.0001 0.0003 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.6703 9.7903 4.8432 10.8642 7.6915 9.1215 13.6111 12.2488 10.0266 12.1304
Translational Efficiency 0.8462 0.8507 1.6192 0.6337 0.9318 0.8221 0.6462 0.8652 0.8646 0.8125

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
ORF
Human Ortholog
Description Protein that may be involved in pH regulation; probable ortholog of A. nidulans PalC, which is involved in pH regulation and binds to the ESCRT-III complex; null mutant does not properly process Rim101p and has decreased resistance to rapamycin; GFP-fusion protein is cytoplasmic; relative distribution to cytoplasm increases upon DNA replication stress
Localization
Cell Percentages cytoplasm (18%), actin (18%), mixed (36%)
Cell Cycle Regulation No
Subcompartmental Group N/A

YGR122W

YGR122W


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ygr122w-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available