Standard name | |
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Human Ortholog | |
Description | Protein serine/threonine phosphatase; regulates Hsp90 chaperone by affecting its ATPase and cochaperone binding activities; has similarity to human phosphatase PP5; present in both the nucleus and cytoplasm; expressed during logarithmic growth |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | – | – | 0 | – | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 2 | 0 | 0 | 0 | – | – | 1 | – | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | – | 0 | – | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | – | 0 | – | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 1 | 0 | 0 | 0 | – | – | 0 | – | – | – | – | 3 | 0 | 2 | 10 | 16 | 7 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 364 | 240 | 25 | 16 | 29 | – | – | 8 | – | – | – | – | 314 | 283 | 304 | 488 | 348 | 340 | 347 | 236 | 58 | 192 | 222 | 223 |
Endoplasmic Reticulum | 0 | 1 | 1 | 0 | 1 | – | – | 1 | – | – | – | – | 4 | 3 | 1 | 6 | 8 | 10 | 2 | 0 | 2 | 1 | 1 | 1 |
Endosome | 0 | 0 | 0 | 0 | 0 | – | – | 0 | – | – | – | – | 0 | 0 | 1 | 0 | 0 | 2 | 2 | 1 | 0 | 0 | 1 | 2 |
Golgi | 2 | 0 | 0 | 0 | 0 | – | – | 0 | – | – | – | – | 0 | 0 | 1 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 1 |
Mitochondria | 7 | 2 | 0 | 0 | 0 | – | – | 1 | – | – | – | – | 1 | 1 | 2 | 5 | 1 | 5 | 2 | 0 | 0 | 1 | 1 | 1 |
Nucleus | 2 | 3 | 0 | 0 | 0 | – | – | 0 | – | – | – | – | 2 | 1 | 2 | 37 | 40 | 28 | 2 | 10 | 0 | 1 | 1 | 7 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | – | – | 0 | – | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | – | – | 1 | – | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | – | 0 | – | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | – | 0 | – | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 4 | 0 | 0 | 2 | – | – | 8 | – | – | – | – | 6 | 18 | 72 | 43 | 20 | 43 | 1 | 4 | 1 | 2 | 1 | 13 |
Unique Cell Count | 371 | 247 | 26 | 16 | 30 | 18 | 323 | 292 | 336 | 524 | 379 | 383 | 367 | 257 | 63 | 204 | 234 | 260 | ||||||
Labelled Cell Count | 376 | 253 | 26 | 16 | 32 | 20 | 330 | 306 | 385 | 589 | 434 | 435 | 367 | 257 | 63 | 204 | 234 | 260 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 12.8 | 15.3 | 10.8 | 9.4 | 9.3 | – | – | 6.8 | – | – | – | – | 13.8 | 13.8 | 13.1 | 15.5 | 18.9 | 18.2 | 12.3 | 12.0 | 12.4 |
Std Deviation (1e-4) | 2.1 | 3.1 | 1.5 | 2.2 | 1.5 | – | – | 1.3 | – | – | – | – | 2.1 | 2.4 | 2.2 | 4.1 | 4.8 | 5.7 | 2.4 | 2.4 | 2.2 |
Intensity Change (Log2) | – | – | – | -0.21 | -0.22 | – | – | -0.67 | – | – | – | – | 0.34 | 0.35 | 0.27 | 0.51 | 0.8 | 0.74 | 0.18 | 0.15 | 0.19 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | – | – | 0 | – | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | – | – | 0 | – | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | – | – | 0 | – | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | – | – | 0 | – | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | – | – | 0 | – | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.8 | 0.1 | – | – | -3.9 | – | – | – | – | 0.3 | 0.2 | -1.0 | -0.6 | -0.8 | -1.2 |
Endoplasmic Reticulum | 0 | 0 | – | – | 0 | – | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | – | – | 0 | – | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | – | – | 0 | – | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | – | – | 0 | – | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | – | – | 0 | – | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | – | – | 0 | – | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | – | – | 0 | – | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | – | – | 0 | – | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | – | – | 0 | – | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | – | – | 0 | – | – | – | – | 0 | 0 | 2.6 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 11.6145 | 15.1359 | 14.0402 | 13.3091 | 13.6309 | 13.8325 | 10.7396 | 13.2693 | 11.655 | 10.969 | 11.1469 | 11.9199 | 11.8385 | 15.4155 | 13.6487 | 13.5882 | 12.0695 | 13.6272 |
Actin | 0.0421 | 0.0007 | 0.0164 | 0.0003 | 0.0002 | 0.0009 | 0.0051 | 0.0002 | 0.008 | 0.0041 | 0.0002 | 0.001 | 0.0082 | 0.0035 | 0.0174 | 0.0118 | 0.0203 | 0.0042 |
Bud | 0.0007 | 0.0029 | 0.0013 | 0.0019 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0014 | 0.0001 | 0.0002 | 0.0006 | 0.0009 | 0.0004 | 0.0003 | 0.0016 | 0.0001 |
Bud Neck | 0.0012 | 0.0002 | 0.001 | 0.0008 | 0.0005 | 0.0012 | 0.0005 | 0.0001 | 0.0013 | 0.0007 | 0.0005 | 0.0011 | 0.001 | 0.0003 | 0.0015 | 0.0007 | 0.0016 | 0.001 |
Bud Periphery | 0.0012 | 0.0005 | 0.0005 | 0.0011 | 0 | 0 | 0.0001 | 0 | 0.0002 | 0.0013 | 0 | 0.0003 | 0.0005 | 0.0003 | 0.0004 | 0.0003 | 0.0018 | 0.0001 |
Bud Site | 0.0021 | 0.001 | 0.0006 | 0.0011 | 0 | 0.0001 | 0.0003 | 0.0001 | 0.0016 | 0.0024 | 0 | 0.0003 | 0.0008 | 0.0009 | 0.0032 | 0.0003 | 0.0027 | 0.0002 |
Cell Periphery | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0.0002 | 0 | 0.0003 | 0 |
Cytoplasm | 0.768 | 0.968 | 0.9173 | 0.9456 | 0.9065 | 0.9157 | 0.8922 | 0.9828 | 0.9345 | 0.8728 | 0.9397 | 0.9375 | 0.7673 | 0.9687 | 0.8726 | 0.8493 | 0.7013 | 0.8757 |
Cytoplasmic Foci | 0.0295 | 0.0019 | 0.0035 | 0.0028 | 0.001 | 0.0133 | 0.0069 | 0.0011 | 0.0034 | 0.008 | 0.0017 | 0.006 | 0.051 | 0.0022 | 0.0127 | 0.013 | 0.049 | 0.0137 |
Eisosomes | 0.0003 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0.0026 | 0 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.022 | 0.0023 | 0.0027 | 0.0015 | 0.0013 | 0.0049 | 0.0077 | 0.0007 | 0.0029 | 0.0032 | 0.0013 | 0.003 | 0.0094 | 0.0006 | 0.0064 | 0.0007 | 0.0071 | 0.0041 |
Endosome | 0.025 | 0.0005 | 0.0027 | 0.0136 | 0.0005 | 0.0084 | 0.0081 | 0.0004 | 0.0022 | 0.0076 | 0.0008 | 0.0075 | 0.0322 | 0.0003 | 0.0151 | 0.0178 | 0.1029 | 0.0141 |
Golgi | 0.0057 | 0.0001 | 0.0015 | 0.0013 | 0 | 0.0003 | 0.0029 | 0 | 0.0012 | 0.0014 | 0 | 0.0012 | 0.0041 | 0.0001 | 0.0051 | 0.0157 | 0.044 | 0.0056 |
Lipid Particles | 0.008 | 0.0001 | 0.0008 | 0 | 0 | 0.0001 | 0.0013 | 0 | 0.0008 | 0.0007 | 0 | 0.0002 | 0.01 | 0 | 0.0022 | 0.0068 | 0.0169 | 0.0072 |
Mitochondria | 0.0027 | 0.0016 | 0.0006 | 0.0056 | 0.0001 | 0.0004 | 0.0009 | 0.0001 | 0.0018 | 0.0027 | 0.0001 | 0.0035 | 0.0027 | 0.0003 | 0.0068 | 0.0107 | 0.0126 | 0.0018 |
None | 0.0047 | 0.004 | 0.0015 | 0.0006 | 0.0012 | 0.0014 | 0.0042 | 0.0006 | 0.0038 | 0.0019 | 0.0009 | 0.0022 | 0.011 | 0.0013 | 0.0057 | 0.0014 | 0.0018 | 0.0024 |
Nuclear Periphery | 0.0238 | 0.0021 | 0.004 | 0.002 | 0.0049 | 0.0054 | 0.0089 | 0.001 | 0.0024 | 0.0091 | 0.0027 | 0.0029 | 0.0111 | 0.002 | 0.0064 | 0.0036 | 0.0041 | 0.0067 |
Nucleolus | 0.0005 | 0.0003 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0002 |
Nucleus | 0.0467 | 0.0117 | 0.0397 | 0.0112 | 0.0821 | 0.0423 | 0.0552 | 0.0123 | 0.0321 | 0.0768 | 0.0505 | 0.0298 | 0.0625 | 0.0158 | 0.0354 | 0.0513 | 0.0168 | 0.0425 |
Peroxisomes | 0.0032 | 0.0001 | 0.004 | 0.0001 | 0 | 0.0002 | 0.0012 | 0 | 0.0015 | 0.0007 | 0 | 0.0002 | 0.0158 | 0.0002 | 0.0029 | 0.0128 | 0.0067 | 0.0103 |
Punctate Nuclear | 0.0054 | 0.0005 | 0.0007 | 0.0002 | 0.0006 | 0.0034 | 0.0028 | 0.0002 | 0.0012 | 0.0015 | 0.0009 | 0.0017 | 0.005 | 0.0022 | 0.0012 | 0.0012 | 0.0018 | 0.0083 |
Vacuole | 0.0044 | 0.0011 | 0.0006 | 0.0072 | 0.0007 | 0.0013 | 0.0009 | 0.0002 | 0.0004 | 0.0028 | 0.0004 | 0.0009 | 0.005 | 0.0002 | 0.0012 | 0.0013 | 0.0048 | 0.0009 |
Vacuole Periphery | 0.0023 | 0.0002 | 0.0002 | 0.003 | 0.0002 | 0.0004 | 0.0003 | 0 | 0.0001 | 0.0006 | 0.0001 | 0.0003 | 0.0015 | 0.0001 | 0.0005 | 0.0009 | 0.0015 | 0.0005 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 55.4564 | 53.0929 | 42.2452 | 52.9407 | 52.4187 | 30.7524 | 45.7592 | 42.5615 | 33.8378 | 49.6082 |
Translational Efficiency | 1.861 | 1.9089 | 2.0661 | 1.3798 | 1.522 | 2.5285 | 1.5704 | 1.6317 | 1.6042 | 1.5738 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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