Standard name
Human Ortholog
Description Asparagine synthetase; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway; ASN2 has a paralog, ASN1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0.07 0.05 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.99 0.98 0.99 0.98 0.96 0.92 – 0.85 0.86 0.86 0.81 0.99 0.99 0.99 0.98 0.99 0.98 0.98 0.95 0.94 0.95 0.94 0.92
Endoplasmic Reticulum 0 0 0 0 0 0 0.07 – 0.12 0.08 0.09 0.11 0 0 0 0 0.06 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 1 2 5 5 0 0 0 0 0 0 0 0 0 0 0 0
Bud 1 1 3 1 0 1 1 – 3 0 0 0 0 0 0 0 0 0 1 1 2 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 3 8 8 7 16 18 – 44 23 12 28 3 2 2 14 5 7 0 0 0 0 0 0
Cytoplasm 343 261 334 319 314 414 439 – 511 395 235 280 155 206 197 341 265 236 317 246 313 65 114 118
Endoplasmic Reticulum 2 4 1 0 2 14 34 – 70 39 25 39 0 0 2 14 15 13 0 1 2 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Golgi 0 1 2 0 0 0 1 – 0 4 1 2 0 1 0 3 4 3 0 0 1 0 0 0
Mitochondria 0 0 0 0 0 0 0 – 0 0 2 0 0 1 0 2 0 0 0 0 0 0 0 0
Nucleus 1 0 0 0 0 0 0 – 3 2 0 2 0 0 0 0 0 0 0 0 1 0 0 1
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 1 0 0 0 0 – 0 1 1 0 0 0 0 1 1 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 1 0 0 0 0 0 0 0 2 0 0 0
Vac/Vac Membrane 0 1 1 2 0 0 1 – 5 11 3 10 0 2 0 1 0 0 0 1 2 0 0 0
Unique Cell Count 345 264 341 321 319 432 479 603 459 274 347 156 208 199 348 269 241 323 260 333 69 122 129
Labelled Cell Count 348 271 350 330 323 445 494 637 477 284 366 159 212 201 376 290 260 323 260 333 69 122 129


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 93.3 121.6 93.8 84.3 72.9 62.5 53.3 – 44.5 40.2 35.7 34.4 98.1 101.6 95.9 142.7 148.5 144.8 83.4 81.0 89.5
Std Deviation (1e-4) 22.1 26.4 20.1 19.1 17.7 12.5 12.9 – 10.6 9.9 9.0 8.4 23.1 23.4 22.9 37.9 39.9 42.2 18.7 28.4 30.0
Intensity Change (Log2) – – – -0.15 -0.36 -0.59 -0.82 – -1.07 -1.22 -1.39 -1.45 0.06 0.11 0.03 0.6 0.66 0.63 -0.17 -0.21 -0.07


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.1 -0.1 1.1 1.1 – 3.2 1.9 1.4 3.4 0 0 0 1.3 -0.4 0.4
Cytoplasm 1.6 0.5 -1.6 -3.8 – -6.4 -5.8 -5.7 -7.3 1.2 1.0 0.9 0 0.5 0
Endoplasmic Reticulum 0 0 2.9 4.8 – 6.3 5.3 5.4 6.1 0 0 0 3.4 4.1 4.0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 – 0 2.4 0 2.7 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 92.7215 110.0522 104.5241 103.6732 92.3775 104.2705 83.3804 103.478 94.9262 85.9821 81.5699 92.2298 70.1746 119.5095 102.5199 94.3004 34.0762 98.8054
Actin 0.0001 0 0.0001 0 0.0002 0.0001 0.0029 0.0003 0.0049 0.0002 0.0026 0.0004 0.0482 0.0001 0.0064 0.0023 0.1264 0
Bud 0.0004 0 0.0003 0 0.0001 0.0001 0.0015 0 0.0002 0.0007 0.0005 0.0003 0.0044 0 0.0018 0.0006 0.0048 0
Bud Neck 0.0002 0.0002 0.0003 0.0004 0.0004 0.0008 0.0006 0.0001 0.0002 0.0002 0.0003 0.0005 0.0018 0.0001 0.0005 0.0002 0.0018 0.0001
Bud Periphery 0.0005 0 0.0001 0 0.0001 0.0001 0.0008 0 0.0004 0.0009 0.0006 0.0001 0.0034 0 0.0013 0.0004 0.0086 0
Bud Site 0.0002 0 0 0 0.0002 0 0.0035 0.0003 0.0002 0.0001 0.0005 0.0002 0.0041 0 0.0052 0.0002 0.0169 0
Cell Periphery 0.0015 0.0002 0.0003 0.0001 0.001 0.0003 0.001 0.0002 0.0003 0.003 0.0008 0.0003 0.001 0 0.0013 0 0.0006 0
Cytoplasm 0.9514 0.9957 0.983 0.9961 0.9352 0.966 0.9219 0.9919 0.9647 0.9193 0.8419 0.9478 0.7611 0.9988 0.9444 0.919 0.3341 0.9979
Cytoplasmic Foci 0.003 0.0001 0.0026 0.0001 0.008 0.0006 0.009 0.0004 0.0006 0.0035 0.0259 0.0097 0.0204 0.0001 0.011 0.0007 0.0254 0.0001
Eisosomes 0.0001 0 0 0 0.0005 0 0.0003 0.0003 0.0002 0.0005 0.0003 0 0.0004 0 0.0001 0 0.0012 0
Endoplasmic Reticulum 0.0011 0.0014 0.0007 0.0004 0.0018 0.0018 0.0052 0.0004 0.0035 0.0007 0.0021 0.0013 0.0094 0.0001 0.002 0.0056 0.004 0.0001
Endosome 0.0005 0 0.0003 0 0.0004 0.0008 0.0022 0.0001 0.0037 0.0013 0.0129 0.0011 0.022 0 0.0048 0.0202 0.0638 0.0001
Golgi 0.0001 0 0.0001 0 0.0002 0 0.0006 0 0.0026 0.0003 0.003 0.0001 0.0102 0 0.0032 0.0014 0.0699 0
Lipid Particles 0.0008 0 0.0012 0 0.0095 0.0002 0.0029 0.0002 0.0007 0.0046 0.0112 0.0009 0.0055 0 0.0036 0.0001 0.0495 0
Mitochondria 0.0036 0 0.0006 0 0.0116 0.0009 0.0059 0.0002 0.004 0.0055 0.0165 0.0028 0.0196 0 0.0047 0.0034 0.2275 0
None 0.0028 0 0.0002 0 0.003 0.0005 0.0092 0.001 0.0009 0.0107 0.0135 0.0053 0.0088 0.0001 0.0016 0.0003 0.0022 0.0001
Nuclear Periphery 0.0038 0.0005 0.0009 0.0005 0.0016 0.0023 0.0054 0.001 0.0061 0.0019 0.0161 0.0019 0.0293 0.0001 0.001 0.0313 0.0048 0.0004
Nucleolus 0.0045 0 0.002 0 0.0128 0.0058 0.0046 0.0006 0.0007 0.0044 0.0105 0.01 0.0051 0 0.0003 0 0.001 0
Nucleus 0.0104 0.0011 0.0021 0.0014 0.0041 0.0075 0.0092 0.0023 0.0019 0.0052 0.006 0.011 0.0229 0.0005 0.0016 0.0042 0.0016 0.0011
Peroxisomes 0.0003 0 0.0002 0 0.0009 0 0.0036 0 0.0001 0.0003 0.0019 0.0001 0.0019 0 0.0017 0.0001 0.04 0
Punctate Nuclear 0.0004 0 0.0003 0 0.0005 0.0002 0.001 0.0002 0.0001 0.0002 0.002 0.0013 0.0076 0 0.0004 0.0003 0.0047 0
Vacuole 0.011 0.0006 0.0033 0.0008 0.0037 0.0095 0.0061 0.0003 0.0021 0.0244 0.0139 0.0029 0.0068 0.0001 0.0022 0.0036 0.0044 0.0001
Vacuole Periphery 0.0034 0 0.0014 0.0001 0.0043 0.0025 0.0027 0.0001 0.002 0.012 0.0172 0.002 0.006 0 0.001 0.0063 0.0068 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 600.4857 563.4796 568.0044 601.534 565.5037 578.0204 614.9874 596.1809 619.1327 575.6704
Translational Efficiency 0.9233 1.0674 1.1132 1.1951 1.0457 1.0071 1.1259 1.2123 1.124 1.1284

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✔
Standard name
Human Ortholog
Description Asparagine synthetase; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway; ASN2 has a paralog, ASN1, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-2

Asn2

Asn2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Asn2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available