Standard name
Human Ortholog
Description Alpha 3 subunit of the 20S proteasome; the only nonessential 20S subunit; may be replaced by the alpha 4 subunit (Pre6p) under stress conditions to create a more active proteasomal isoform
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.05 0.07 0.14 0.13 0.14 0.15 0.1 0.2 0.06 0.11 0.16 0 0.11 0.09 0 0.06 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.09 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0.1 0 0.13 0.1 0.11 0.14 0.06 0.1 0.06 0 0.06 0 0.07 0 0.07 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.54 0.72 0.74 0.75 0.47 0.34 0.35 0.34 0.4 0.36 0.25 0.24 0.58 0.45 0.42 0.65 0.46 0.33 0.7 0.67 0.76 0.7 0.74 0.61
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.26 0 0 0 0 0.07 0 0 0.07 0.07 0.19 0.17 0.06 0 0 0 0 0.07 0.13 0.06 0.13 0.1 0.05 0.09
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.28 0.36 0.21 0.18 0.46 0.51 0.46 0.48 0.41 0.38 0.4 0.4 0.32 0.42 0.38 0.32 0.39 0.51 0.11 0.15 0.07 0.12 0.12 0.22
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 1 0 2 0 0 0 1 0 0 0 0 0 0 0 0 1 0
Bud 0 0 0 0 0 0 0 0 0 2 2 0 0 0 0 0 0 0 0 0 2 0 4 7
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1 1 4 2 9 10 25 35 16 23 5 17 3 17 29 6 17 21 1 3 0 0 3 3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 2 9 20 0 0 0 1 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 2 0
Golgi 1 0 16 4 22 15 19 37 7 16 3 3 3 5 13 1 11 9 0 0 0 0 0 0
Mitochondria 0 1 0 0 0 0 0 1 1 1 4 3 0 0 0 0 3 2 0 1 0 0 0 0
Nucleus 21 18 121 77 80 51 62 89 47 57 13 20 29 71 76 90 73 74 64 45 167 35 152 144
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 1 0 0 2 0 0 0 0 0 2 0 1 1
Nucleolus 10 0 4 4 3 10 7 7 8 11 10 14 3 4 4 4 2 15 11 4 29 5 10 22
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 11 9 34 19 79 77 82 128 48 60 21 33 16 66 68 44 62 114 9 9 16 6 23 53
Unique Cell Count 39 25 164 103 171 151 179 264 118 158 52 83 50 158 179 138 158 222 92 67 221 51 205 238
Labelled Cell Count 44 29 179 106 193 163 196 298 127 172 58 91 55 165 192 147 177 256 92 67 221 51 205 238


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 36.9 30.1 38.3 38.1 38.9 35.7 33.0 32.6 31.1 30.6 26.4 25.7 36.9 37.6 36.0 29.5 33.4 32.2 35.6 38.2 42.3
Std Deviation (1e-4) 8.2 9.9 7.4 7.8 8.2 8.2 11.2 8.2 8.2 8.3 8.0 9.4 8.4 12.0 10.8 8.8 11.6 12.0 9.0 12.3 13.0
Intensity Change (Log2) – – – -0.01 0.02 -0.1 -0.22 -0.23 -0.3 -0.33 -0.54 -0.58 -0.05 -0.03 -0.09 -0.38 -0.2 -0.25 -0.11 -0.01 0.14


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 1.3 1.8 3.8 3.8 3.6 3.9 0 4.8 0 3.0 4.3 0 3.0 2.8
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3.9
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi -1.8 0.9 0.1 0.3 1.3 -1.2 0.1 0 -1.7 0 -2.4 -0.8 -3.4 -0.9 -2.3
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.2 -5.0 -7.1 -7.3 -8.1 -5.7 -6.8 -6.3 -7.5 -2.1 -5.3 -5.9 -1.6 -5.1 -7.9
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 1.8 0.8 0 1.8 1.9 0 4.1 0 0 0 0 0 1.9
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -0.5 4.9 5.6 4.9 5.8 3.6 3.4 2.8 3.2 1.6 4.1 3.5 2.2 3.6 6.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 30.947 34.3103 28.5501 29.9006 26.8619 29.3893 31.2221 41.7473 36.8188 32.8956 31.9246 35.0732 27.5062 36.1202 32.6374 28.731 33.0681 33.4744
Actin 0.0005 0.0001 0.0213 0.0003 0.0396 0.0001 0.0096 0 0.0018 0.0069 0.0016 0.0043 0.029 0 0.0007 0.0193 0.0001 0.0098
Bud 0.0001 0 0.0002 0.0001 0.0001 0.0003 0.0003 0 0.0002 0.0002 0.0001 0.0002 0.0012 0.0001 0 0.0003 0 0.0001
Bud Neck 0.0001 0.0001 0.0001 0.0001 0.0007 0.0004 0.0001 0 0.0001 0.0001 0.0002 0.0007 0.0004 0 0.0001 0.0025 0.0001 0.0011
Bud Periphery 0.0001 0 0.0003 0.0001 0.0001 0.0002 0.0006 0 0.0002 0.0003 0.0001 0.0002 0.0016 0.0001 0 0.0002 0 0.0002
Bud Site 0.0002 0 0.0004 0 0.0003 0.0001 0.0004 0 0.0003 0.0003 0.0001 0.0002 0.0044 0 0.0001 0.0047 0 0.0001
Cell Periphery 0.0006 0 0.0001 0 0.0001 0.0003 0.0003 0 0.0001 0.0001 0.0001 0.0001 0.0006 0.0001 0 0.0001 0 0.0001
Cytoplasm 0.004 0.0008 0.0009 0.0008 0.001 0.0025 0.0138 0.0003 0.0033 0.0011 0.0025 0.0035 0.0076 0.0007 0.001 0.0013 0.0004 0.0009
Cytoplasmic Foci 0.0056 0 0.0088 0 0.0025 0.0006 0.0122 0 0.0018 0.0047 0.0019 0.0023 0.0144 0.0001 0.0007 0.0276 0 0.0008
Eisosomes 0 0 0.0001 0 0.0002 0 0.0002 0 0 0.0001 0 0 0.0003 0 0 0.0002 0 0
Endoplasmic Reticulum 0.0011 0 0.0002 0 0.0016 0.0001 0.0022 0 0.0008 0.0014 0.0013 0.0011 0.002 0 0.0002 0.0004 0 0.0007
Endosome 0.0036 0 0.0028 0 0.0078 0.0002 0.0093 0 0.0038 0.0219 0.0224 0.0088 0.0138 0.0001 0.0025 0.0092 0 0.0029
Golgi 0.0003 0 0.0048 0 0.0038 0 0.0071 0 0.0006 0.008 0.0137 0.0026 0.0054 0 0.0004 0.0128 0 0.003
Lipid Particles 0.0024 0 0.0063 0 0.0047 0.0001 0.0136 0 0.0006 0.0071 0.0078 0.0012 0.0076 0.0001 0.0005 0.0405 0 0.0005
Mitochondria 0.0024 0.0004 0.0071 0.0009 0.001 0.0022 0.0118 0.0003 0.0035 0.0217 0.004 0.0027 0.0076 0.0005 0.0011 0.0018 0.0007 0.0014
None 0.0024 0 0.0006 0 0.0015 0.0008 0.0123 0 0.0015 0.0006 0.0011 0.0015 0.0032 0.0001 0.007 0.001 0 0.0001
Nuclear Periphery 0.028 0.0212 0.0274 0.0235 0.0126 0.013 0.0211 0.0092 0.0151 0.0305 0.0613 0.0339 0.0248 0.007 0.0158 0.0107 0.0086 0.0253
Nucleolus 0.0197 0.0048 0.0125 0.0058 0.0281 0.0224 0.0224 0.0038 0.0122 0.0417 0.0258 0.0153 0.0237 0.004 0.0147 0.0016 0.0136 0.0259
Nucleus 0.9185 0.9718 0.8991 0.9667 0.886 0.9466 0.8305 0.986 0.9485 0.8408 0.8449 0.9146 0.8232 0.9786 0.952 0.8449 0.9759 0.9229
Peroxisomes 0.0002 0 0.0033 0 0.001 0.0001 0.0025 0 0.0004 0.0049 0.0004 0.0006 0.0034 0 0.0002 0.0136 0 0.0001
Punctate Nuclear 0.0048 0.0001 0.001 0.0003 0.0063 0.0037 0.0233 0 0.0035 0.0042 0.0061 0.0038 0.0179 0 0.0022 0.0067 0.0001 0.0003
Vacuole 0.0031 0.0003 0.0012 0.0003 0.0003 0.0031 0.004 0.0001 0.0008 0.0009 0.0016 0.0009 0.005 0.0027 0.0002 0.0002 0.0001 0.0023
Vacuole Periphery 0.0023 0.0004 0.0016 0.0007 0.0007 0.0032 0.0024 0.0002 0.0008 0.0025 0.0031 0.0018 0.003 0.0058 0.0005 0.0003 0.0003 0.0013

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 154.789 153.6056 160.1399 177.7854 145.4548 178.8314 161.4073 180.6266 188.5712 162.9262
Translational Efficiency 1.6216 1.5127 1.4015 1.4777 1.3463 1.4307 1.5642 1.3072 1.3356 1.3216

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Alpha 3 subunit of the 20S proteasome; the only nonessential 20S subunit; may be replaced by the alpha 4 subunit (Pre6p) under stress conditions to create a more active proteasomal isoform
Localization
Cell Percentages nucleus (86%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-5

Pre9

Pre9


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pre9-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available