Standard name
Human Ortholog
Description Negative regulator of actin nucleation-promoting factor activity; interacts with Las17p, a homolog of human Wiskott-Aldrich Syndrome protein (WASP), via an N-terminal SH3 domain, and along with PIN3 cooperatively inhibits the nucleation of actin filaments; overexpression blocks receptor-mediated endocytosis; protein increases in abundance and forms nuclear foci in response to DNA replication stress; LSB1 has a paralog, PIN3, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.96 0.93 0.96 0.83 0.88 0.77 0.82 – 0.79 0.71 0.71 0.76 0.95 0.97 0.97 0.76 0.83 0.88 0.94 0.88 0.95 0.9 0.9 0.86
Endoplasmic Reticulum 0 0 0 0 0.05 0 0.06 – 0.07 0.06 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.08 0.05 0.05 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.18 0.1 0.19 0.11 – 0.09 0.18 0.2 0.16 0 0 0 0.22 0.15 0.07 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 4 0 – 2 3 3 0 0 0 0 0 0 1 0 2 1 0 1 0
Bud 0 3 1 0 0 3 0 – 0 1 3 0 0 0 0 0 0 0 1 3 1 0 4 3
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 1 0 0 2 1
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 5 6 0 6 2 13 – 12 3 8 7 4 5 2 2 5 4 0 0 0 0 0 0
Cytoplasm 184 319 323 165 217 207 326 – 278 274 195 214 374 601 529 348 254 290 183 319 332 233 365 282
Endoplasmic Reticulum 7 1 1 5 13 4 24 – 26 23 10 15 8 9 8 10 11 7 0 1 1 5 5 6
Endosome 0 2 0 0 0 0 0 – 1 1 0 1 1 1 0 2 1 2 1 3 0 1 2 4
Golgi 1 0 0 0 0 0 0 – 0 1 0 0 1 1 1 38 15 15 2 0 0 1 0 0
Mitochondria 0 2 0 0 0 8 0 – 14 9 11 8 1 0 1 10 3 9 0 9 4 1 0 0
Nucleus 0 1 3 0 5 3 10 – 7 11 12 6 8 7 3 3 3 1 0 9 1 5 10 10
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 1 0 0 1 0 0 0 0 0 1 0 3 1 2
Nucleolus 0 1 0 0 0 0 0 – 1 1 1 0 0 0 0 5 3 2 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 1 0 0 0 1 4 0 2 0 0 0 0 0 4
Vac/Vac Membrane 5 15 14 36 25 50 43 – 30 71 54 46 5 13 7 101 47 24 1 4 2 3 4 7
Unique Cell Count 191 343 337 199 247 270 396 350 385 276 282 392 617 547 459 307 331 195 363 351 261 404 328
Labelled Cell Count 197 349 348 206 267 281 416 371 398 299 297 402 638 552 523 342 357 195 363 351 261 404 328


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 16.3 12.0 15.2 15.3 16.8 11.4 13.9 – 13.0 13.1 11.8 12.8 17.2 17.9 18.6 27.6 29.9 26.8 14.2 16.0 16.4
Std Deviation (1e-4) 3.4 2.7 2.6 2.6 2.9 2.8 2.7 – 2.6 2.9 2.6 2.5 3.5 3.3 3.4 10.7 11.4 10.5 3.4 3.4 4.2
Intensity Change (Log2) – – – 0.01 0.15 -0.42 -0.12 – -0.22 -0.21 -0.37 -0.24 0.19 0.24 0.29 0.86 0.98 0.82 -0.09 0.08 0.11


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0.5 0 1.3 – 1.4 0 0.9 0.6 0 0 0 0 -0.1 0
Cytoplasm -5.1 -3.6 -7.1 -5.7 – -6.5 -8.7 -8.6 -7.3 -0.3 1.3 0.7 -7.7 -5.4 -3.9
Endoplasmic Reticulum 0 3.9 0 4.3 – 4.8 4.2 0 3.9 0 0 0 0 3.1 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 5.4 4.1 4.0
Mitochondria 0 0 0 0 – 3.7 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 1.7 – 0 1.9 2.8 0 1.3 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 5.4 2.9 5.7 3.4 – 2.4 5.9 6.0 5.1 -2.4 -1.8 -2.7 7.1 4.8 1.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 10.0092 10.9703 11.2483 8.799 8.379 11.1232 11.4783 15.4213 15.0484 14.218 14.859 15.2579 19.0734 21.7786 22.3614 20.131 22.5485 22.273
Actin 0.0431 0.0159 0.003 0.0733 0.0182 0.0003 0.0232 0.0027 0.0023 0.0031 0.0001 0.0016 0.0009 0.0102 0.0165 0.0135 0.0568 0.0251
Bud 0.0008 0.0006 0.001 0.0023 0.0003 0.0001 0.0006 0.0025 0.0002 0.0143 0.0002 0.0049 0.0001 0.0006 0.0008 0.0026 0.0001 0.0002
Bud Neck 0.0095 0.0006 0.0015 0.0011 0.0009 0.0023 0.0052 0.0006 0.0014 0.0013 0.0015 0.0134 0.0002 0.0012 0.0007 0.0005 0.0008 0.0127
Bud Periphery 0.0009 0.0003 0.0006 0.0017 0.0003 0 0.0004 0.0006 0.0001 0.0041 0.0001 0.0021 0 0.0001 0.0002 0.0012 0 0
Bud Site 0.0076 0.0039 0.0011 0.0266 0.0008 0 0.0027 0.0038 0.0005 0.0003 0.0001 0.0013 0.0001 0.0014 0.0006 0.0022 0.0001 0.0001
Cell Periphery 0.0006 0.0001 0.0002 0.0004 0.0001 0 0.0002 0.0001 0.0001 0 0.0001 0.0002 0 0 0 0 0 0
Cytoplasm 0.7594 0.9294 0.8897 0.762 0.618 0.9271 0.6815 0.9489 0.9218 0.9214 0.9455 0.9022 0.9866 0.8974 0.8503 0.7616 0.6832 0.852
Cytoplasmic Foci 0.0101 0.0201 0.0413 0.0272 0.1201 0.0306 0.0585 0.0129 0.0138 0.0012 0.001 0.0089 0.0006 0.0624 0.0932 0.1519 0.1722 0.0706
Eisosomes 0.0002 0 0 0.0003 0.0001 0 0.0002 0 0.0001 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.016 0.0009 0.0009 0.0046 0.0121 0.0012 0.0085 0.001 0.0031 0.0011 0.0022 0.0026 0.0006 0.0006 0.0006 0.0004 0.0023 0.001
Endosome 0.0306 0.0024 0.0122 0.0168 0.0314 0.0013 0.0462 0.0007 0.0029 0.0002 0.0005 0.0062 0.0001 0.001 0.0008 0.0007 0.045 0.0008
Golgi 0.0277 0.0008 0.0077 0.021 0.0809 0.0002 0.0068 0.0002 0.001 0 0 0.0018 0 0.0009 0.0006 0.0009 0.0034 0.0005
Lipid Particles 0.0055 0.0005 0.0077 0.0191 0.0419 0.0009 0.0159 0.0001 0.0022 0 0 0.0006 0 0.0003 0.0005 0.0002 0.0022 0.0006
Mitochondria 0.0071 0.0007 0.0059 0.0108 0.0083 0.0001 0.0053 0.0002 0.0022 0.0003 0.0001 0.0011 0.0001 0.0002 0.0004 0.0003 0.0003 0.0003
None 0.0029 0.0013 0.0017 0.002 0.0036 0.0009 0.0106 0.0017 0.0025 0.0031 0.0008 0.002 0.0014 0.0013 0.0013 0.0016 0.0008 0.0008
Nuclear Periphery 0.0398 0.0014 0.0012 0.003 0.0287 0.0024 0.0204 0.0015 0.004 0.0035 0.0044 0.0042 0.0012 0.0009 0.0016 0.0011 0.0013 0.0015
Nucleolus 0.0015 0.0001 0.0001 0.0002 0.0004 0 0.0008 0.0001 0.0002 0.0001 0.0001 0.0015 0 0 0 0 0.0001 0
Nucleus 0.0226 0.0146 0.0129 0.0084 0.0095 0.0245 0.0363 0.0189 0.0363 0.0416 0.0376 0.0393 0.0077 0.0083 0.0186 0.0184 0.007 0.0216
Peroxisomes 0.003 0.0031 0.006 0.0141 0.0128 0.0031 0.0468 0.0017 0.002 0.0001 0 0.0004 0 0.0114 0.0088 0.0112 0.0123 0.0043
Punctate Nuclear 0.0046 0.001 0.002 0.0039 0.0085 0.0046 0.0267 0.0016 0.0026 0.0036 0.0006 0.0042 0.0003 0.0015 0.0042 0.0315 0.01 0.0078
Vacuole 0.0028 0.0022 0.0025 0.0009 0.0016 0.0003 0.0023 0.0003 0.0006 0.0005 0.0048 0.0009 0.0001 0.0001 0.0001 0.0001 0.0013 0.0001
Vacuole Periphery 0.0036 0.0003 0.0009 0.0003 0.0016 0.0001 0.0011 0 0.0002 0.0001 0.0002 0.0003 0 0 0 0 0.0007 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 40.2758 38.0421 32.2615 78.2399 41.3813 30.38 62.8479 84.3726 74.0483 64.6419
Translational Efficiency 2.2883 2.8206 3.5387 1.3439 2.6065 4.5282 2.0201 1.8492 2.1685 2.0203

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Negative regulator of actin nucleation-promoting factor activity; interacts with Las17p, a homolog of human Wiskott-Aldrich Syndrome protein (WASP), via an N-terminal SH3 domain, and along with PIN3 cooperatively inhibits the nucleation of actin filaments; overexpression blocks receptor-mediated endocytosis; protein increases in abundance and forms nuclear foci in response to DNA replication stress; LSB1 has a paralog, PIN3, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (91%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Lsb1

Lsb1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Lsb1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available