Standard name
Human Ortholog
Description v-SNARE binding protein; facilitates specific protein retrieval from a late endosome to the Golgi; modulates arginine uptake, possible role in mediating pH homeostasis between the vacuole and plasma membrane H(+)-ATPase; contributes to prion curing; preferentially expressed after severe ethanol stress
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.87 0.94 0.96 0.97 0.93 0.83 0.79 0.79 0.75 0.71 0.61 0.6 0.99 0.99 0.99 0.82 0.85 0.82 0.73 0.88 0.75 0.94 0.81 0.66
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.21 0.11 0 0.07 0.14 0.36 0.4 0.36 0.45 0.5 0.64 0.58 0 0 0 0 0 0 0.14 0 0.11 0 0.05 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.15 0.12 0.15 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 1 0 1 0 0 6 0 3 3
Bud 1 2 0 3 5 7 26 11 13 15 15 12 0 0 2 0 2 0 0 0 4 0 8 30
Bud Neck 0 0 1 0 0 0 1 1 2 4 0 1 1 0 3 0 0 0 1 0 1 0 2 2
Bud Site 0 0 0 0 0 1 2 0 1 6 2 4 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 0 1 6 5 2 2 2 3 1 2 2 2 1 2 5 3 2 0 0 0 0 1 0
Cytoplasm 93 137 320 237 324 362 482 429 339 375 276 260 383 469 516 203 126 129 103 129 253 223 329 257
Endoplasmic Reticulum 1 2 1 0 0 0 0 1 0 0 1 0 0 2 1 8 4 9 2 0 2 0 1 0
Endosome 0 1 3 0 7 5 3 6 2 0 0 0 1 0 0 11 4 7 3 1 8 0 6 25
Golgi 0 0 3 1 1 1 1 1 0 0 0 0 0 1 0 9 3 4 1 0 4 0 2 9
Mitochondria 23 16 8 16 47 155 246 194 203 262 289 248 1 0 0 5 2 6 19 4 38 3 20 15
Nucleus 1 0 0 0 1 1 6 3 3 8 2 4 0 0 2 0 0 1 0 0 0 0 1 1
Nuclear Periphery 0 0 0 0 0 0 1 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 1 0 2 1 4 1 3 2 2 0 0 1 0 0 0 15 4 5 0 0 0 0 0 1
Peroxisomes 0 0 1 1 0 0 0 1 1 0 0 3 0 0 0 0 1 0 0 0 0 0 1 2
SpindlePole 0 0 0 1 4 4 14 6 13 7 4 12 0 0 0 2 1 1 4 3 5 0 12 25
Vac/Vac Membrane 2 0 4 1 4 8 10 13 13 7 9 8 1 3 1 38 18 23 2 2 4 1 6 8
Unique Cell Count 107 145 334 244 348 436 609 544 455 526 453 430 385 474 521 248 149 157 142 147 340 237 405 393
Labelled Cell Count 123 158 344 267 402 547 798 670 595 685 601 556 389 476 528 297 168 188 142 147 340 237 405 393


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.3 4.0 4.9 4.6 4.7 3.6 3.4 3.9 3.2 2.9 2.6 2.7 5.4 5.7 5.0 11.6 9.9 10.4 5.2 5.5 6.4
Std Deviation (1e-4) 1.9 1.6 5.5 4.9 6.2 4.2 2.4 3.8 2.7 1.3 1.6 1.2 1.1 5.2 1.1 10.8 8.1 8.7 1.2 1.9 2.4
Intensity Change (Log2) – – – -0.1 -0.05 -0.43 -0.54 -0.33 -0.59 -0.74 -0.89 -0.86 0.14 0.22 0.04 1.25 1.02 1.1 0.08 0.17 0.39


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 6.5 5.3 6.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.6025 1.144 0.6868 0.6822 0.2921 0.8184 0.1466 -0.6882 -0.6082 -0.5556 -0.7749 -0.7033 -0.5073 -0.988 -0.8275 -0.8254 -0.7471 -1.0356
Actin 0.047 0.0001 0.0026 0.042 0.0021 0.0019 0.0769 0.0066 0.0413 0.0009 0.0001 0.0009 0.039 0.0003 0.0088 0.0133 0.0274 0.0056
Bud 0.0015 0 0.0003 0.0002 0.0004 0.0013 0.0025 0.0042 0.0094 0.0007 0 0 0.0038 0.0037 0.0017 0.004 0.0008 0.0005
Bud Neck 0.0101 0 0.0003 0.0027 0.0004 0.0002 0.0034 0.0001 0.0008 0.0003 0 0.0003 0.0067 0.0001 0.0036 0.0007 0.0486 0.0007
Bud Periphery 0.0039 0 0.0005 0.0006 0.0004 0.0025 0.0054 0.0051 0.0054 0.0014 0 0.0001 0.0155 0.0047 0.0075 0.0108 0.0011 0.0016
Bud Site 0.0045 0.0001 0.0052 0.0003 0.0081 0.0002 0.0147 0.0069 0.0218 0.0005 0 0.0001 0.0119 0.0041 0.0078 0.0023 0.0193 0.0006
Cell Periphery 0.0005 0 0.0001 0.0003 0.0002 0 0.0005 0.0003 0.0003 0.0001 0 0 0.0014 0.0002 0.0005 0.0002 0.0006 0.0001
Cytoplasm 0.0156 0.0337 0.0363 0.0188 0.0277 0.0583 0.0557 0.0952 0.0873 0.0293 0.0295 0.1721 0.0345 0.0681 0.0909 0.0473 0.0405 0.0979
Cytoplasmic Foci 0.0155 0.0068 0.0053 0.0017 0.0139 0.0113 0.0378 0.0063 0.0066 0.0294 0.0052 0.0107 0.0328 0.0045 0.017 0.0254 0.021 0.0248
Eisosomes 0.001 0.0001 0.0003 0.0028 0.0002 0.0001 0.0022 0.0004 0.0002 0.0002 0 0.0001 0.0006 0.0001 0.0005 0.0002 0.0004 0.0001
Endoplasmic Reticulum 0.0019 0.0002 0.0005 0.0008 0.0016 0.0002 0.0048 0.0006 0.0007 0.0012 0.0003 0.0018 0.0024 0.0005 0.0015 0.0007 0.0049 0.001
Endosome 0.0064 0.0006 0.001 0.0025 0.0159 0.0007 0.0221 0.0016 0.001 0.0074 0.0726 0.0037 0.0194 0.0005 0.0129 0.0019 0.0528 0.0043
Golgi 0.0034 0.0001 0.0005 0.0032 0.0013 0.0002 0.0177 0.0031 0.0015 0.0019 0.0203 0.0012 0.011 0.0001 0.007 0.0018 0.0388 0.0027
Lipid Particles 0.0059 0.0005 0.0027 0.0036 0.0034 0.0003 0.039 0.0004 0.0011 0.0055 0.0011 0.003 0.0274 0.0004 0.0084 0.0065 0.0203 0.003
Mitochondria 0.0112 0.0001 0.0004 0.0025 0.0005 0.0002 0.0363 0.0029 0.0014 0.0008 0.0013 0.0111 0.0116 0.0003 0.003 0.0022 0.0373 0.0015
None 0.8429 0.9384 0.9364 0.9076 0.9008 0.9099 0.5965 0.863 0.8125 0.8756 0.8668 0.7789 0.7214 0.9097 0.8054 0.8338 0.6501 0.8419
Nuclear Periphery 0.0024 0.0001 0.0007 0.0024 0.0097 0.0002 0.0113 0.0002 0.0003 0.0058 0.0002 0.0022 0.0111 0.0002 0.0027 0.0011 0.0111 0.0005
Nucleolus 0.0011 0.0001 0.0006 0.0009 0.0006 0.0002 0.0013 0.0003 0.0004 0.0033 0 0.0001 0.0022 0.0004 0.0012 0.0013 0.0058 0.0003
Nucleus 0.0017 0.0002 0.0006 0.0007 0.0019 0.008 0.01 0.0005 0.0011 0.0131 0.0001 0.0101 0.0029 0.0006 0.0039 0.0042 0.0014 0.0008
Peroxisomes 0.0164 0.0003 0.0022 0.0036 0.0008 0.0033 0.0467 0.001 0.0048 0.0117 0.0002 0.0014 0.0323 0.0003 0.0111 0.0296 0.0096 0.0056
Punctate Nuclear 0.0056 0.0183 0.0031 0.0023 0.009 0.0006 0.0102 0.0005 0.0013 0.0092 0.0002 0.0011 0.0049 0.0006 0.0025 0.0115 0.0016 0.006
Vacuole 0.001 0.0001 0.0003 0.0002 0.0007 0.0006 0.0031 0.0006 0.0006 0.0014 0.001 0.0011 0.0057 0.0006 0.0014 0.0008 0.0039 0.0006
Vacuole Periphery 0.0005 0 0.0001 0.0003 0.0004 0.0001 0.0019 0.0001 0.0001 0.0004 0.0008 0.0003 0.0015 0.0001 0.0006 0.0003 0.0027 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 19.2402 52.7987 11.8724 15.5169 13.9232 16.0992 43.0718 35.7581 29.9217 16.7471
Translational Efficiency 0.6795 0.7608 0.6625 0.9425 0.9761 1.1024 0.9745 0.8621 0.7634 0.8966

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description v-SNARE binding protein; facilitates specific protein retrieval from a late endosome to the Golgi; modulates arginine uptake, possible role in mediating pH homeostasis between the vacuole and plasma membrane H(+)-ATPase; contributes to prion curing; preferentially expressed after severe ethanol stress
Localization
Cell Percentages cytoplasm (35%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Btn2

Btn2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Btn2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available