ORF
Human Ortholog
Description Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF RSR1/BUD1/YGR152C; relative distribution to the nucleus increases upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0.05 0.07 0 0 0 0 0 0 0 0 0
Cytoplasm 0.92 0.89 0.89 0.79 0.78 0.71 – 0.63 0.47 0.52 0.48 0.41 0.96 0.93 0.94 0.88 0.93 0.89 0.77 0.78 0.78 0.7 0.79 0.78
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0.05 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.16 0.23 0.05 0.25 – 0.38 0.54 0.54 0.53 0.6 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0.07 0 – 0.08 0.1 0.07 0.09 0.07 0 0 0 0 0.06 0 0.05 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.05 0.16 0 0.05 0.16 0.14 – 0.09 0.09 0.1 0 0 0 0 0 0.06 0.1 0.1 0.09 0.1 0.07 0.15 0.11 0.1
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 0 0 0 – 0 0 0 0 2 0 0 0 0 0 0 7 2 2 1 1 3
Bud 1 0 9 4 0 3 – 6 11 6 6 15 0 0 1 0 1 0 9 2 2 4 3 5
Bud Neck 2 0 0 0 0 0 – 0 0 1 0 0 0 0 0 0 0 0 18 2 2 3 4 4
Bud Site 0 0 0 0 0 0 – 1 1 0 1 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 1 1 2 – 4 4 4 9 10 6 12 24 2 3 1 2 0 2 0 0 0
Cytoplasm 693 231 213 157 224 317 – 317 229 249 191 177 239 244 314 97 77 109 692 208 211 148 213 234
Endoplasmic Reticulum 13 1 1 3 2 3 – 1 0 2 6 23 5 7 6 5 1 2 2 3 5 1 2 1
Endosome 1 1 0 0 3 3 – 1 3 0 0 0 1 2 1 0 0 1 8 0 1 0 4 4
Golgi 1 0 0 0 1 0 – 0 0 0 0 0 0 1 0 0 1 2 2 0 0 0 2 2
Mitochondria 7 0 38 46 14 113 – 192 261 259 208 261 0 0 1 4 2 2 3 3 9 3 2 4
Nucleus 28 2 1 7 19 16 – 41 48 33 34 31 4 8 4 3 5 5 43 9 4 6 2 2
Nuclear Periphery 1 1 0 0 0 0 – 2 3 1 2 3 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 2 0 0 0 0 0 0 0 0 0 0 0 1 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 1 0 0 0 0 0 0 0 8 0 2 1 0 1
Vac/Vac Membrane 41 43 9 10 45 64 – 44 46 47 16 17 6 5 9 7 8 12 83 27 18 32 30 30
Unique Cell Count 752 261 239 198 287 444 503 485 481 395 435 249 262 333 110 83 123 900 268 273 212 272 300
Labelled Cell Count 789 279 271 228 309 521 609 606 604 474 540 261 279 360 118 98 134 900 268 273 212 272 300


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.0 5.9 4.1 4.7 5.6 4.7 – 4.4 4.0 4.4 4.1 4.1 6.2 6.4 6.2 7.9 7.4 8.3 6.1 6.1 6.2
Std Deviation (1e-4) 0.6 1.0 1.2 1.5 2.0 1.9 – 1.2 1.3 1.4 1.2 0.8 1.1 1.2 1.1 1.8 1.5 2.1 1.4 1.1 1.8
Intensity Change (Log2) – – – 0.21 0.46 0.2 – 0.12 -0.01 0.11 0.03 0.02 0.61 0.66 0.6 0.97 0.87 1.03 0.57 0.58 0.6


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 3.3 4.2 0 0 0
Cytoplasm -2.8 -3.4 -5.3 – -7.4 -10.9 -9.8 -10.3 -12.2 2.9 1.6 2.3 -0.3 1.0 -0.1
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 -4.2 0 – 0 0 0 0 0 -6.6 -6.7 -7.3 -3.3 -3.2 -4.1
Nucleus 0 3.7 2.5 – 4.3 4.8 3.8 4.4 3.9 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0.7 4.5 4.3 – 2.5 2.7 2.8 0.2 0.1 -0.9 -1.3 -0.7 1.1 0 2.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.0354 2.9178 2.9269 2.6456 2.39 2.3462 1.6943 2.5862 2.535 2.3651 2.1068 2.461 2.6964 3.7764 3.4459 4.0232 3.8794 3.3794
Actin 0.0235 0.0041 0.0268 0.019 0.0345 0.0013 0.0211 0.0021 0.0217 0.0064 0.0364 0.0023 0.0113 0.0007 0.0089 0.0033 0.0063 0.0008
Bud 0.0007 0.0003 0.0015 0.0011 0.0004 0.0001 0.001 0.0041 0.0007 0.0008 0.0045 0.0004 0.0004 0.0004 0.0003 0.0006 0.0003 0.0002
Bud Neck 0.0038 0.0012 0.0042 0.002 0.0024 0.0049 0.0018 0.0031 0.0043 0.0025 0.0051 0.0245 0.0042 0.0013 0.0022 0.0458 0.0039 0.0173
Bud Periphery 0.0011 0.0002 0.0018 0.0021 0.0006 0.0001 0.0013 0.001 0.0008 0.0007 0.0055 0.0006 0.0005 0.0002 0.0002 0.0009 0.0002 0.0002
Bud Site 0.003 0.0075 0.0043 0.0032 0.0007 0.0002 0.007 0.0163 0.0134 0.0013 0.0111 0.0006 0.0019 0.0037 0.0037 0.0064 0.0007 0.0007
Cell Periphery 0.0006 0.0004 0.0004 0.0003 0.0003 0.0001 0.0006 0.0006 0.0007 0.0003 0.0006 0.0004 0.0005 0.0004 0.0003 0.0005 0.0003 0.0002
Cytoplasm 0.6834 0.677 0.7132 0.651 0.7079 0.844 0.7155 0.7092 0.7065 0.7483 0.6297 0.7657 0.7044 0.7156 0.6059 0.6043 0.7385 0.6778
Cytoplasmic Foci 0.0118 0.0069 0.0165 0.013 0.0113 0.01 0.0153 0.0057 0.0167 0.0107 0.0217 0.0085 0.0067 0.0051 0.0058 0.0079 0.0172 0.0043
Eisosomes 0.0002 0.0001 0.0001 0.0001 0.0002 0 0.0003 0 0.0001 0 0.0003 0 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0231 0.0096 0.0153 0.0073 0.0168 0.0116 0.0176 0.0114 0.0148 0.0162 0.015 0.0089 0.018 0.0073 0.0147 0.0298 0.0232 0.0081
Endosome 0.0145 0.003 0.0147 0.0209 0.0296 0.0068 0.0242 0.0059 0.0128 0.0494 0.0298 0.0106 0.0118 0.0019 0.0061 0.0146 0.0205 0.0029
Golgi 0.0028 0.0004 0.0046 0.0065 0.004 0.0004 0.0058 0.0005 0.0081 0.0028 0.0211 0.0029 0.0021 0.0002 0.0008 0.0007 0.0028 0.0002
Lipid Particles 0.0033 0.0004 0.0021 0.0027 0.0061 0.0003 0.0098 0.0005 0.0064 0.0015 0.0132 0.0066 0.0037 0.0003 0.0007 0.0039 0.0034 0.0001
Mitochondria 0.0012 0.0003 0.0067 0.0143 0.0028 0.0005 0.0062 0.0006 0.0059 0.0025 0.0413 0.0029 0.0013 0.0003 0.0005 0.001 0.0004 0.0003
None 0.1594 0.1447 0.0582 0.0816 0.0982 0.0379 0.1052 0.0796 0.0458 0.0128 0.041 0.0537 0.121 0.0642 0.0567 0.0507 0.0723 0.0649
Nuclear Periphery 0.0133 0.0063 0.0091 0.0046 0.0346 0.0093 0.0107 0.0075 0.0081 0.0097 0.0229 0.0046 0.0134 0.0065 0.0104 0.0233 0.0083 0.0057
Nucleolus 0.0005 0.0006 0.0006 0.0005 0.0012 0.0002 0.0008 0.0015 0.0007 0.0006 0.0008 0.0005 0.0005 0.0005 0.001 0.0025 0.0012 0.0006
Nucleus 0.0384 0.1286 0.1036 0.1409 0.0302 0.0656 0.0358 0.1396 0.1135 0.1073 0.0728 0.0887 0.0884 0.1835 0.2711 0.1796 0.0591 0.2059
Peroxisomes 0.0018 0.0003 0.0051 0.0163 0.0016 0.0004 0.005 0.0002 0.0087 0.0012 0.0091 0.0086 0.0011 0.0002 0.0009 0.0004 0.0005 0.0001
Punctate Nuclear 0.0082 0.0056 0.0071 0.0031 0.0109 0.004 0.0077 0.0062 0.0059 0.0034 0.0097 0.0045 0.004 0.0055 0.0056 0.0191 0.0369 0.0073
Vacuole 0.005 0.0025 0.0034 0.008 0.0036 0.0018 0.0062 0.0041 0.004 0.0189 0.0043 0.0035 0.0041 0.0021 0.004 0.0042 0.0038 0.0021
Vacuole Periphery 0.0006 0.0002 0.0007 0.0016 0.0022 0.0007 0.0012 0.0003 0.0004 0.0025 0.004 0.001 0.0006 0.0001 0.0003 0.0004 0.0003 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 47.6172 8.8069 10.8919 32.3061 48.9644 45.9497 15.5832 14.0235 16.7317 46.7903
Translational Efficiency 1.9044 2.4292 1.7122 0.9414 1.1116 1.8061 1.0085 0.9602 1.0878 1.2215

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
ORF
Human Ortholog
Description Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF RSR1/BUD1/YGR152C; relative distribution to the nucleus increases upon DNA replication stress
Localization
Cell Percentages cytoplasm (27%), nucleus (3%), mixed (44%)
Cell Cycle Regulation No
Subcompartmental Group N/A

YGR151C

YGR151C


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ygr151c-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available