Standard name
Human Ortholog
Description Phosphatidylethanolamine methyltransferase (PEMT); catalyzes the first step in the conversion of phosphatidylethanolamine to phosphatidylcholine during the methylation pathway of phosphatidylcholine biosynthesis
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.1 0.17 0.21 0.22 0.14 0.19 0.16 0.1 – 0.18 0.22 0.23 0.07 0.09 0.06 0.29 0.24 0.2 0 0 0.05 0.08 0.08 0
Bud 0 0.06 0 0 0 0 0 0 – 0 0.05 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.18 0.2 0.28 0.26 0.14 0.13 0.12 0.07 – 0.16 0.17 0.18 0.26 0.25 0.3 0.36 0.33 0.35 0.06 0.07 0.15 0.13 0.17 0.17
Cytoplasm 0.12 0.13 0.05 0.06 0.08 0.12 0.08 0.09 – 0.18 0.2 0.21 0.08 0.08 0.11 0.22 0.26 0.21 0 0.05 0 0 0 0
Endoplasmic Reticulum 0.8 0.71 0.75 0.68 0.75 0.74 0.8 0.78 – 0.64 0.53 0.5 0.82 0.81 0.77 0 0 0 0.87 0.81 0.76 0.73 0.68 0.71
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0.09 0.13 0.15 0 0.05 0 – 0 0 0 0 0 0 0.17 0.24 0.36 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.06 0 0 – 0.06 0.16 0.16 0 0 0 0.08 0.08 0.05 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 42 51 94 60 42 85 88 50 – 78 74 93 14 18 18 163 84 53 14 10 24 21 29 18
Bud 7 18 7 8 9 12 11 12 – 17 17 13 1 1 2 27 13 9 0 0 0 0 2 2
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 1 1 0 0 0 1
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 76 60 124 69 42 58 63 37 – 71 56 73 50 50 86 201 116 94 27 26 67 35 65 87
Cytoplasm 50 38 22 16 23 53 43 46 – 78 66 86 15 15 32 121 90 57 7 16 1 1 2 6
Endoplasmic Reticulum 333 215 334 182 224 322 435 401 – 280 178 201 160 161 221 15 7 9 387 281 347 206 263 354
Endosome 0 0 0 1 0 0 0 0 – 0 0 0 0 0 0 4 0 1 0 0 0 0 0 1
Golgi 16 0 41 34 44 11 25 14 – 16 3 7 3 7 0 95 85 97 1 1 5 4 8 12
Mitochondria 1 0 1 12 3 25 19 21 – 27 53 66 0 0 0 46 27 13 1 1 3 5 7 7
Nucleus 0 0 1 0 0 0 0 0 – 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 3 2 – 3 3 8 0 0 0 0 0 0 0 1 0 0 0 0
Nucleolus 0 0 1 0 0 1 0 1 – 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 1 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 1 3 0 0 0 0 0 1 0
Vac/Vac Membrane 2 1 2 1 1 3 7 6 – 3 4 5 0 0 1 13 5 6 3 5 0 2 1 3
Unique Cell Count 418 302 447 267 300 436 546 511 440 337 404 196 200 286 560 349 271 447 349 455 282 390 501
Labelled Cell Count 527 383 627 383 388 571 694 590 573 454 552 243 252 360 687 431 339 447 349 455 282 390 501


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 27.9 27.5 24.1 25.9 27.6 24.4 26.6 25.0 – 22.5 20.5 20.0 28.9 28.9 27.8 18.6 21.7 25.1 27.5 29.5 29.4
Std Deviation (1e-4) 5.6 5.2 4.8 4.7 5.8 5.2 5.5 5.4 – 4.6 3.9 4.1 5.6 5.6 5.5 4.5 6.2 6.6 6.8 6.9 6.3
Intensity Change (Log2) – – – 0.1 0.19 0.02 0.14 0.05 – -0.1 -0.24 -0.27 0.26 0.26 0.21 -0.37 -0.15 0.06 0.19 0.29 0.29


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0.5 -2.4 -0.6 -2.0 -4.9 – -1.2 0.3 0.7 -4.3 -3.7 -5.4 2.9 1.0 -0.5
Bud 1.3 1.3 1.2 0.5 0.9 – 2.1 2.8 1.6 0 0 0 2.8 1.9 1.5
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery -0.6 -4.4 -5.3 -6.5 -8.5 – -4.2 -3.7 -3.3 -0.6 -0.7 0.7 2.7 1.7 2.0
Cytoplasm 0.6 1.5 3.9 1.9 2.5 – 6.0 6.4 7.2 1.4 1.3 3.2 7.5 8.4 6.7
Endoplasmic Reticulum -1.9 0 -0.3 1.9 1.4 – -3.6 -6.4 -7.5 1.9 1.6 0.8 -23.9 -20.6 -18.6
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Golgi 1.5 2.3 -4.2 -2.9 -4.3 – -3.4 -5.0 -4.7 -3.5 -2.5 -5.3 3.6 5.8 8.8
Mitochondria 0 0 4.8 3.6 4.0 – 5.0 8.5 8.7 0 0 0 6.0 5.7 4.3
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 – 0 0 0 0 0 0 2.4 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 19.7366 23.8982 23.4688 23.5241 19.9786 23.5394 24.29 27.8141 27.1376 24.3686 26.0797 26.2353 31.0299 28.6985 29.9258 29.1608 26.2358 28.7331
Actin 0.0619 0.0152 0.0497 0.009 0.0064 0.0131 0.0212 0.0048 0.0305 0.0053 0.017 0.01 0.0136 0.0054 0.0105 0.0052 0.0051 0.005
Bud 0.0012 0.0029 0.0057 0.0021 0.0002 0.0002 0.0047 0.0003 0.0018 0.0002 0.0003 0.0005 0.0002 0.0003 0.0005 0.0004 0.0001 0.0001
Bud Neck 0.004 0.0026 0.0058 0.004 0.0215 0.0123 0.0011 0.0017 0.0032 0.0019 0.0046 0.0078 0.001 0.0015 0.0022 0.0173 0.0025 0.0065
Bud Periphery 0.004 0.0039 0.0224 0.0117 0.0015 0.0012 0.0077 0.0019 0.0135 0.0028 0.0044 0.0032 0.0009 0.0013 0.003 0.0034 0.0006 0.0012
Bud Site 0.0063 0.0306 0.0158 0.0009 0.0002 0.0002 0.0032 0.0057 0.0018 0.0003 0.0002 0.0003 0.0005 0.0024 0.0022 0.0012 0 0.0001
Cell Periphery 0.0607 0.0891 0.0765 0.0752 0.0214 0.027 0.0458 0.1091 0.0848 0.0492 0.0224 0.0282 0.0555 0.0749 0.0759 0.0731 0.0379 0.03
Cytoplasm 0.0297 0.122 0.0572 0.0171 0.044 0.0271 0.0156 0.0447 0.0212 0.0058 0.0066 0.0128 0.0111 0.0311 0.0185 0.0123 0.0144 0.0083
Cytoplasmic Foci 0.0153 0.0022 0.003 0.0022 0.0007 0.0013 0.0058 0.001 0.0015 0.0004 0.005 0.0013 0.004 0.001 0.0008 0.0011 0.0026 0.0007
Eisosomes 0.0034 0.0035 0.0023 0.0016 0.0007 0.0033 0.0051 0.0085 0.0038 0.0049 0.0047 0.0096 0.0081 0.0055 0.0034 0.0042 0.0055 0.0043
Endoplasmic Reticulum 0.6447 0.6532 0.688 0.7807 0.8255 0.8717 0.7999 0.7744 0.7886 0.7912 0.8118 0.8904 0.8565 0.8245 0.8487 0.8343 0.864 0.9067
Endosome 0.0306 0.0027 0.0076 0.0083 0.0042 0.0037 0.0109 0.0053 0.0034 0.0023 0.0117 0.0049 0.0023 0.0021 0.0021 0.0043 0.008 0.003
Golgi 0.0136 0.0086 0.01 0.0146 0.0037 0.0212 0.0108 0.0109 0.01 0.0173 0.0739 0.0117 0.0088 0.016 0.0064 0.0139 0.0463 0.0203
Lipid Particles 0.0248 0.0388 0.0113 0.0053 0.0031 0.006 0.0146 0.0104 0.012 0.0022 0.0124 0.0027 0.0051 0.0178 0.0081 0.0095 0.0029 0.0049
Mitochondria 0.0063 0.0025 0.0064 0.0023 0.0031 0.0006 0.0059 0.0012 0.0054 0.1 0.0018 0.0015 0.0007 0.0014 0.0005 0.0011 0.0005 0.0005
None 0.0038 0.0003 0.0012 0.0035 0.0008 0.0002 0.0018 0.0004 0.0005 0.0006 0.0017 0.0003 0.0031 0.0002 0.0004 0.0002 0.0002 0.0002
Nuclear Periphery 0.0358 0.0098 0.0135 0.0239 0.0477 0.0065 0.0218 0.0093 0.0095 0.0081 0.0069 0.007 0.0152 0.0084 0.0089 0.0091 0.0061 0.0045
Nucleolus 0.0011 0.0001 0.0012 0.001 0.0006 0 0.0007 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0002 0.0001 0 0
Nucleus 0.0167 0.0009 0.0055 0.01 0.0073 0.0004 0.0027 0.0005 0.0006 0.0005 0.0003 0.0006 0.0007 0.0003 0.0015 0.0006 0.0002 0.0003
Peroxisomes 0.0077 0.0001 0.0013 0.0007 0 0 0.0101 0 0.0002 0.0004 0.0066 0.0007 0.0003 0 0 0.0001 0 0
Punctate Nuclear 0.0075 0.0002 0.0027 0.0133 0.0014 0.0001 0.0037 0.0001 0.0002 0 0.0051 0.0005 0.0066 0.0001 0.0006 0.0001 0 0.0001
Vacuole 0.0168 0.0101 0.0112 0.0103 0.003 0.003 0.0051 0.0088 0.0063 0.0051 0.0015 0.0047 0.0048 0.0052 0.0052 0.0074 0.0024 0.0029
Vacuole Periphery 0.0042 0.0006 0.0018 0.0024 0.0031 0.0006 0.0018 0.0007 0.0011 0.0014 0.001 0.001 0.0009 0.0006 0.0006 0.0011 0.0006 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 190.2421 168.1752 204.4366 261.5011 180.0598 158.4474 163.0682 199.8747 202.8771 226.9856
Translational Efficiency 0.6571 0.5636 0.566 0.4011 0.4355 0.8391 0.598 0.4057 0.4692 0.424

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Phosphatidylethanolamine methyltransferase (PEMT); catalyzes the first step in the conversion of phosphatidylethanolamine to phosphatidylcholine during the methylation pathway of phosphatidylcholine biosynthesis
Localization
Cell Percentages ER (75%)
Cell Cycle Regulation No
Subcompartmental Group ER-5

Cho2

Cho2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cho2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available