Standard name
Human Ortholog
Description Mitochondrial ribosomal protein of the small subunit; null mutant does not grow on glycerol, is sensitive to 2,4-dichlorophenol, and accumulates large lipid droplets
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.07 0.05 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.15 0.13 0.07 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0.05 0 0.16 0.05 0 0 0 0 0 0 0 0.07 0.12 0.05 0.07 0.06 0 0.08 0 0.05 0.05 0.06
Golgi 0 0 0.1 0.11 0 0 0 0 0 0 0 0 0.19 0.24 0.23 0.41 0.38 0.13 0 0.06 0 0 0.05 0
Mitochondria 0.96 0.91 0.73 0.91 0.8 0.85 0.9 0.82 0.95 0.9 0.95 0.9 0.83 0.66 0.67 0.05 0.09 0.1 0.83 0.69 0.82 0.77 0.69 0.65
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.08 0 0 0 0 0.06 0.08 0.08 0.1 0.05 0.07 0.08 0.08 0.05 0.06 0 0 0 0 0 0 0 0 0
Nucleolus 0.05 0 0 0 0 0 0.06 0.07 0.06 0.06 0.09 0.05 0 0 0 0 0.05 0.06 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0.07 0.12 0.12 0.36 0 0 0 0.07 0.1 0.08
SpindlePole 0 0 0.05 0 0.05 0 0 0 0 0 0 0 0 0 0 0.05 0.09 0.08 0 0 0 0 0 0
Vac/Vac Membrane 0.09 0.16 0.12 0 0.15 0.11 0.08 0.06 0 0.08 0.05 0.07 0.17 0.24 0.17 0.14 0.09 0.2 0 0.05 0 0 0 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 2 8 0 0 0 0 3 1 0 2 2 2 1 0 2 4 2 1 1 2 1 4 9
Bud 6 1 3 5 5 8 10 9 12 8 8 4 0 1 2 5 6 4 0 1 0 0 1 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 5
Bud Site 2 0 0 0 0 0 4 3 5 4 4 3 0 0 0 4 2 1 – – – – – –
Cell Periphery 3 2 2 7 4 6 6 2 5 2 3 3 4 4 6 9 12 6 0 0 0 0 0 0
Cytoplasm 3 2 4 4 7 9 8 11 4 9 8 6 4 5 3 2 2 2 0 0 1 0 0 0
Endoplasmic Reticulum 3 2 1 0 0 3 0 2 2 2 0 1 4 5 11 1 2 0 1 0 0 0 0 0
Endosome 3 0 5 4 48 19 14 10 5 3 0 6 9 17 30 3 6 5 13 11 3 7 14 24
Golgi 0 1 10 20 13 3 0 2 1 2 0 2 39 58 56 24 35 11 3 8 2 6 16 15
Mitochondria 282 122 71 169 246 302 405 312 400 456 329 335 172 156 165 3 8 8 242 104 91 114 212 250
Nucleus 2 4 1 0 1 7 7 13 6 10 0 2 1 0 2 1 1 1 0 2 0 0 2 1
Nuclear Periphery 23 1 0 4 9 22 35 32 44 27 24 31 16 11 14 0 1 1 7 5 0 1 3 7
Nucleolus 16 2 2 2 5 10 26 27 25 30 30 20 4 6 6 1 5 5 1 0 0 0 5 3
Peroxisomes 4 0 2 6 20 6 3 4 4 5 0 2 2 6 17 7 11 30 12 2 1 10 30 29
SpindlePole 4 0 5 3 16 11 10 11 7 15 3 8 5 3 6 3 8 7 2 1 0 0 2 6
Vac/Vac Membrane 26 22 12 8 46 40 36 21 16 40 18 26 34 57 41 8 8 17 2 7 4 3 10 22
Unique Cell Count 295 134 97 185 309 357 452 379 422 504 348 372 206 238 248 59 91 83 292 151 111 150 308 384
Labelled Cell Count 377 161 126 232 420 446 564 462 537 613 429 451 296 330 359 73 111 100 292 151 111 150 308 384


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.1 7.0 6.7 6.9 6.7 5.8 5.4 6.2 5.4 5.8 5.2 5.9 7.7 7.7 7.3 8.0 9.2 8.7 6.8 7.6 7.6
Std Deviation (1e-4) 0.7 1.2 2.2 1.8 1.4 1.3 1.2 1.3 1.1 1.2 1.2 1.3 1.2 1.2 1.1 1.5 1.8 1.9 1.4 1.7 1.7
Intensity Change (Log2) – – – 0.03 -0.01 -0.21 -0.32 -0.13 -0.31 -0.23 -0.38 -0.18 0.19 0.19 0.12 0.24 0.45 0.36 0.01 0.18 0.18


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 -1.1 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 2.9 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 2.6 0.1 0 0 0 0 0 0 0 0.7 1.9 0 0.4 0
Golgi 0.1 -2.3 0 0 0 0 0 0 0 1.9 2.9 2.6 4.5 4.5 0.6
Mitochondria 4.1 1.3 2.6 4.3 2.0 6.6 4.7 6.2 4.3 2.1 -1.4 -1.2 -8.3 -8.9 -8.6
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 2.8 3.0 3.3 0 2.7 2.9 2.8 0 0 0 0 0
Nucleolus 0 0 0 0 1.9 1.5 1.6 2.2 0 0 0 0 0 0 0
Peroxisomes 0 1.7 0 0 0 0 0 0 0 0 0 1.8 0 2.7 6.0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 1.0 0.9
Vacuole -2.5 0.6 -0.3 -1.4 -2.4 -3.4 -1.4 -2.5 -1.7 0.9 2.4 1.0 0.2 -0.8 1.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 7.5292 6.8648 6.7595 6.7648 5.7748 7.0333 6.8033 7.1929 7.0693 6.1383 5.705 6.6073 6.213 5.8929 5.651 5.3945 5.7693 5.7517
Actin 0.0093 0.0098 0.0097 0.0383 0.0015 0.0011 0.0112 0.003 0.0059 0.0459 0.0236 0.0007 0.0286 0.0118 0.0107 0.0015 0.023 0.0019
Bud 0.0003 0.0005 0.0002 0.0004 0.0001 0 0.0004 0.0002 0.0001 0.0002 0.0013 0 0.0005 0.0013 0.0001 0.0001 0.0006 0
Bud Neck 0.0043 0.0004 0.0001 0.0026 0 0.0001 0.0008 0.0001 0.0002 0.0011 0.0005 0 0.0008 0.0002 0.0001 0.0004 0.0004 0.0001
Bud Periphery 0.0005 0.001 0.0003 0.0008 0.0002 0.0001 0.0008 0.0004 0.0003 0.0004 0.003 0.0001 0.0008 0.0015 0.0002 0.0003 0.0013 0.0001
Bud Site 0.0038 0.0013 0.0012 0.0028 0.0001 0.0001 0.0015 0.0004 0.0004 0.0011 0.0053 0 0.0036 0.0014 0.0003 0.0012 0.0028 0
Cell Periphery 0.0003 0.0002 0.0001 0.0003 0 0.0001 0.0002 0.0001 0.0001 0.0002 0.0002 0.0001 0.0004 0.0002 0.0001 0.0003 0.0002 0
Cytoplasm 0.0033 0.0001 0.0001 0.0002 0.0001 0.0001 0.0015 0.0001 0.0009 0.0001 0.0006 0 0.001 0.004 0.0002 0.0012 0.0001 0.0078
Cytoplasmic Foci 0.0144 0.0034 0.009 0.0218 0.002 0.0033 0.0186 0.0026 0.0021 0.0024 0.005 0.0004 0.0097 0.0114 0.0024 0.0016 0.0022 0.0008
Eisosomes 0.0003 0.0002 0.0002 0.0004 0.0002 0.0001 0.0005 0.0001 0.0002 0.0005 0.0004 0.0001 0.0007 0.0006 0.0004 0.0001 0.0004 0.0001
Endoplasmic Reticulum 0.0018 0.0001 0.0002 0.0002 0.0003 0.0003 0.0052 0.0003 0.0007 0.0004 0.0012 0 0.0011 0.0002 0.0001 0.0086 0.0001 0.0008
Endosome 0.0295 0.0167 0.0058 0.0079 0.0055 0.0083 0.0228 0.0257 0.0108 0.0077 0.0271 0.0044 0.0196 0.0127 0.0037 0.0112 0.0092 0.0047
Golgi 0.0212 0.055 0.0168 0.0123 0.0101 0.017 0.0266 0.035 0.0196 0.0129 0.0117 0.018 0.0239 0.049 0.0148 0.0041 0.0155 0.0269
Lipid Particles 0.027 0.0025 0.0134 0.0125 0.0048 0.0037 0.0261 0.0059 0.0074 0.0068 0.0188 0.0012 0.0399 0.0073 0.0068 0.0047 0.0043 0.0084
Mitochondria 0.8482 0.8862 0.9287 0.8892 0.9338 0.9508 0.8414 0.9083 0.921 0.9152 0.857 0.9715 0.8102 0.8695 0.9469 0.9417 0.9268 0.9424
None 0.0009 0 0.0001 0.0002 0.0002 0 0.005 0.0001 0.0018 0.0001 0.0025 0 0.0009 0.0001 0.0004 0.0003 0.0001 0
Nuclear Periphery 0.0049 0.0001 0.0002 0.0001 0.031 0.0011 0.0046 0.0003 0.0024 0.0002 0.009 0 0.0022 0.0001 0.0001 0.0077 0.0004 0.0001
Nucleolus 0.0017 0.0005 0.0001 0.0001 0.0002 0.0001 0.0006 0.0001 0.0004 0.0001 0.0013 0 0.007 0.0001 0.0001 0.0004 0.0011 0
Nucleus 0.0035 0.0003 0.0001 0.0001 0.0005 0.0002 0.0011 0.0001 0.0008 0.0001 0.0015 0 0.0012 0.0001 0.0001 0.0008 0.0009 0
Peroxisomes 0.0151 0.014 0.0117 0.0067 0.0045 0.0059 0.0163 0.0029 0.0056 0.0029 0.014 0.0013 0.0239 0.0216 0.0089 0.0072 0.0067 0.0027
Punctate Nuclear 0.0021 0 0.0002 0.0002 0.0031 0.0001 0.0087 0 0.0016 0.0002 0.0052 0 0.0012 0.0003 0.001 0.0001 0.0002 0
Vacuole 0.0044 0.0047 0.0005 0.0013 0.0004 0.0025 0.0016 0.0063 0.0052 0.0005 0.0061 0.0003 0.0097 0.0015 0.0007 0.0046 0.0015 0.0004
Vacuole Periphery 0.0034 0.003 0.001 0.0016 0.0014 0.0051 0.0046 0.008 0.0122 0.0011 0.0044 0.0017 0.013 0.0052 0.0022 0.002 0.0023 0.0025

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 23.9177 19.0053 21.8593 26.7883 33.0777 21.2484 28.8244 29.506 28.942 30.6876
Translational Efficiency 1.2255 1.7131 1.39 1.2228 0.9256 1.6775 1.2724 1.2245 1.3762 1.3985

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Mitochondrial ribosomal protein of the small subunit; null mutant does not grow on glycerol, is sensitive to 2,4-dichlorophenol, and accumulates large lipid droplets
Localization
Cell Percentages mitochondrion (98%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Mrps35

Mrps35


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mrps35-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available