Standard name
Human Ortholog
Description Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; regulates endocytic progression; thought to regulate clathrin function; the clathrin triskelion is a trimeric molecule composed of three heavy chains that radiate from a vertex and three light chains which bind noncovalently near the vertex of the triskelion
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.14 0.14 0.09 0.06 0 0.08 0 0 0.06 0 0 0.08 0.14 0.09 0.05 0.14 0.14 0.15 0.09 0.2 0.2 0.19 0.15 0.14
Bud 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.06 0.05 0.05 0 0
Golgi 0.59 0.48 0.28 0.36 0.28 0.12 0.1 0.11 0.09 0.1 0.05 0.06 0.71 0.68 0.8 0.65 0.7 0.68 0.69 0.6 0.63 0.58 0.66 0.67
Mitochondria 0.56 0.7 0.82 0.81 0.78 0.9 0.87 0.84 0.9 0.86 0.92 0.9 0.43 0.32 0.2 0.13 0.09 0.11 0.06 0.05 0 0.05 0.06 0.05
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.09 0.06 0.06 0.06 0.08 0.09 0.12 0.15 0.15 0.14 0.13 0.14 0.05 0.11 0.1 0.19 0.15 0.17 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.08 0.05 0.06
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.11 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.08 0 0.06 0.05 0 0.05 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 86 46 26 11 7 31 21 18 26 18 22 51 65 43 27 48 44 38 65 72 69 79 61 72
Bud 15 0 9 4 6 10 4 8 20 10 14 11 8 3 2 12 5 2 6 1 3 1 1 2
Bud Neck 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Bud Site 1 1 0 0 0 0 0 0 4 2 2 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 0 1 0 1 0 2 1 0 0 0 1 1 1 2 1 1 0 0 0 0 0 0 0
Cytoplasm 1 3 4 4 11 9 8 10 9 4 6 4 7 5 6 12 10 13 0 0 0 0 0 0
Endoplasmic Reticulum 15 9 2 6 7 14 12 9 6 6 1 13 10 10 11 11 3 5 5 2 0 2 0 2
Endosome 1 1 4 0 0 2 2 3 0 4 1 2 1 1 6 9 6 4 47 23 17 20 17 21
Golgi 351 156 85 70 70 48 49 56 39 45 27 39 328 343 450 227 222 168 481 221 216 245 266 344
Mitochondria 337 228 246 158 196 362 426 440 391 383 456 542 198 162 110 46 29 27 40 19 8 21 22 25
Nucleus 1 1 0 0 0 0 0 0 0 1 0 0 0 0 0 2 0 0 1 0 0 0 0 0
Nuclear Periphery 0 1 1 3 0 2 5 1 2 3 0 0 0 1 1 2 0 1 1 1 0 1 1 1
Nucleolus 54 19 18 11 21 36 58 78 66 61 64 87 25 54 54 67 47 42 5 1 0 1 1 0
Peroxisomes 0 1 12 3 3 3 6 3 5 14 5 3 3 6 7 7 8 1 8 3 20 34 20 29
SpindlePole 1 0 2 0 1 0 1 0 0 1 0 1 2 2 1 6 5 0 3 0 2 1 0 1
Vac/Vac Membrane 63 13 2 2 7 1 10 11 3 6 4 5 14 35 43 15 18 13 29 17 1 8 5 6
Unique Cell Count 599 325 299 195 252 402 487 525 434 444 497 601 461 502 561 349 319 248 699 370 342 419 401 511
Labelled Cell Count 928 479 412 272 330 518 604 638 571 558 602 759 662 666 720 465 398 315 699 370 342 419 401 511


Late Golgi

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 25.3 24.0 20.5 20.9 19.8 19.5 20.2 20.2 20.5 19.7 19.9 20.5 26.8 25.3 29.2 25.0 28.4 26.8 26.3 26.8 27.4
Std Deviation (1e-4) 6.3 5.9 5.7 5.8 4.9 4.7 5.6 5.6 6.0 5.6 6.0 6.0 7.4 7.6 8.8 10.0 9.5 9.8 6.1 6.3 6.6
Intensity Change (Log2) – – – 0.03 -0.05 -0.08 -0.02 -0.02 -0.0 -0.06 -0.05 -0.0 0.38 0.3 0.51 0.28 0.47 0.39 0.36 0.38 0.42


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -1.3 -2.9 -0.5 -2.5 -3.2 -1.4 -2.6 -2.5 -0.1 2.2 -0.1 -2.3 2.0 2.0 2.4
Bud -0.7 -0.5 -0.4 0 -1.4 1.1 -0.6 -0.2 -1.1 -1.2 0 0 0.3 -1.2 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 2.2 0.9 0 0.6 0.7 0 0 0 0 0 0 1.7 1.5 2.6
Endoplasmic Reticulum 0 0 2.5 1.8 0 0 0 0 0 0 0 0 2.2 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 1.1 0 0
Golgi 1.7 -0.2 -5.5 -6.6 -6.5 -6.9 -6.4 -9.0 -9.0 11.6 10.9 14.9 9.3 10.2 9.2
Mitochondria -0.4 -1.3 3.0 2.0 0.6 3.1 1.5 4.0 3.4 -10.7 -13.7 -17.8 -17.6 -18.3 -16.6
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -0.2 1.1 1.4 2.7 3.8 3.8 3.3 3.1 3.7 -0.3 2.3 1.8 5.0 3.5 4.1
Peroxisomes -1.6 -2.0 -3.0 -2.5 -3.6 -2.5 -0.6 -2.8 0 -3.3 -2.6 -2.6 -1.5 -1.1 -2.8
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 2.2 4.1 4.4 2.9 3.5 3.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 29.7206 28.2042 28.4011 27.5436 27.9643 27.9111 31.5347 31.7883 30.424 28.0595 29.6888 30.6167 28.057 29.9345 27.2562 28.5867 20.9219 28.3107
Actin 0.0463 0.0266 0.0327 0.0031 0.0033 0.0117 0.065 0.0263 0.0286 0.0035 0.009 0.0092 0.0721 0.05 0.0494 0.0045 0.4053 0.0048
Bud 0.0002 0.0008 0.0003 0 0.0002 0.0002 0.0002 0.0003 0.0002 0.0002 0.0001 0 0.0003 0.0024 0.0001 0.0006 0.0012 0
Bud Neck 0.0004 0.0005 0.0005 0.0002 0.0004 0.0002 0.0005 0.0004 0.0003 0.0004 0.0001 0.0002 0.0005 0.0009 0.0002 0.0005 0.0019 0.0002
Bud Periphery 0.0003 0.0006 0.0004 0 0.0003 0.0003 0.0002 0.0003 0.0002 0.0003 0.0001 0.0001 0.0003 0.0015 0.0001 0.0009 0.0014 0.0001
Bud Site 0.0003 0.001 0.0004 0.0001 0.0003 0.0002 0.0003 0.0006 0.0002 0.0003 0.0001 0.0001 0.0008 0.0086 0.0001 0.0005 0.0014 0
Cell Periphery 0.0018 0.0011 0.0012 0.0003 0.0006 0.0005 0.0005 0.0004 0.0003 0.0009 0.0002 0.0001 0.0002 0.0005 0.0002 0.0009 0.0006 0.0002
Cytoplasm 0.0008 0.0015 0.0006 0.0008 0.0021 0.0004 0.0019 0.0006 0.0004 0.0004 0.0002 0.0001 0.0006 0.004 0.0004 0.0006 0.0004 0.0001
Cytoplasmic Foci 0.0141 0.0246 0.0127 0.0206 0.0242 0.0081 0.0135 0.0156 0.0126 0.0142 0.0109 0.0065 0.0134 0.0241 0.017 0.0186 0.0171 0.0038
Eisosomes 0.0008 0.0003 0.0005 0.0001 0.0001 0.0003 0.0011 0.0004 0.0004 0.0004 0.0002 0.0002 0.0004 0.0003 0.0001 0 0.0016 0.0002
Endoplasmic Reticulum 0.0006 0.0006 0.0006 0.0008 0.001 0.0006 0.0006 0.0006 0.0005 0.0005 0.0004 0.0003 0.0009 0.0005 0.0005 0.0006 0.0005 0.001
Endosome 0.0971 0.1072 0.0996 0.1797 0.2016 0.0537 0.0707 0.0845 0.0886 0.0856 0.0561 0.0353 0.1097 0.1376 0.1133 0.169 0.0724 0.0556
Golgi 0.6163 0.6865 0.721 0.7401 0.6044 0.8481 0.6476 0.7251 0.7504 0.7546 0.8383 0.8558 0.7507 0.6132 0.7175 0.452 0.3375 0.8245
Lipid Particles 0.0513 0.0431 0.042 0.0199 0.0264 0.0149 0.032 0.0298 0.0147 0.0429 0.0313 0.0111 0.0119 0.044 0.0298 0.0682 0.0185 0.0161
Mitochondria 0.125 0.0735 0.0468 0.0095 0.0955 0.0469 0.1436 0.0904 0.0857 0.082 0.0404 0.0766 0.0243 0.0609 0.0541 0.0439 0.1269 0.0665
None 0.0001 0 0 0 0 0 0.0039 0 0 0 0 0 0.0002 0.0001 0 0.0001 0.0001 0.0001
Nuclear Periphery 0.0002 0.0002 0.0002 0.0003 0.0003 0.0002 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001 0.0004 0.0004 0.0002 0.0012 0.0002 0.0105
Nucleolus 0.001 0.0002 0.0052 0 0.0003 0.0001 0.0001 0.0002 0.0001 0.0003 0.0001 0 0 0.0002 0.0001 0.0009 0.0002 0.0001
Nucleus 0.0005 0.0001 0.0006 0 0.0002 0.0001 0.0002 0.0001 0 0.0001 0 0 0.0001 0.0002 0.0001 0.0005 0.0002 0.0001
Peroxisomes 0.0025 0.0032 0.0008 0.0006 0.0013 0.0006 0.0057 0.0034 0.0017 0.0011 0.0056 0.0012 0.0028 0.0035 0.0007 0.0012 0.0047 0.0001
Punctate Nuclear 0 0.0001 0 0 0 0 0.0001 0 0 0 0 0 0.0003 0.0001 0 0.0001 0.0001 0.0001
Vacuole 0.018 0.0113 0.0173 0.0077 0.0116 0.0059 0.0023 0.0083 0.0034 0.0038 0.0015 0.0004 0.0024 0.0186 0.0035 0.0677 0.0037 0.0015
Vacuole Periphery 0.0225 0.0167 0.0166 0.0161 0.0259 0.0071 0.0099 0.0127 0.0113 0.0082 0.0053 0.0025 0.0078 0.0283 0.0125 0.1674 0.0042 0.0144

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 99.0235 99.7617 106.3493 132.1043 116.1608 109.9652 125.0207 147.6659 154.4111 136.5449
Translational Efficiency 3.8863 3.9381 3.2414 2.934 3.3338 4.1213 2.8888 2.837 2.508 2.6031

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; regulates endocytic progression; thought to regulate clathrin function; the clathrin triskelion is a trimeric molecule composed of three heavy chains that radiate from a vertex and three light chains which bind noncovalently near the vertex of the triskelion
Localization
Cell Percentages —
Cell Cycle Regulation No
Subcompartmental Group N/A

Clc1

Clc1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Clc1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available