Standard name
Human Ortholog
Description Protein kinase involved in the cell cycle checkpoint into anaphase; in complex with Mad1p and Bub3p, prevents progression into anaphase in presence of spindle damage; Cdc28p-mediated phosphorylation at Bub1p-T566 is important for degradation in anaphase and adaptation of checkpoint to prolonged mitotic arrest; associates with centromere DNA via Skp1p; involved in Sgo1p relocalization in response to sister kinetochore tension; paralog MAD3 arose from whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0.06 0.06 0.1 0.08 0.07 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.64 0.54 0.8 0.71 0.67 0.69 0.63 0.66 0.58 0.58 0.54 0.47 0.6 0.67 0.73 0.24 0.24 0.34 0.33 0.24 0.39 0.6 0.62 0.64
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0.08 0 0 0 0 0 0
Endosome 0 0.08 0 0 0 0 0 0 0 0 0 0 0.23 0.2 0.11 0.21 0.13 0.21 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.15 0.08 0.15 0.12 0.26 0.31 0.34 0.54 0.47 0.56 0.61 0 0 0 0 0 0 0.08 0.08 0.11 0 0.05 0.07
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.13 0.21 0.19 0 0.06 0 0.05 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0.08 0.09 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.17 0.12 0.12 0.15 0.21 0.14 0.15 0.14 0.07 0.12 0 0.09 0.19 0.15 0.08 0.27 0.29 0.17 0.28 0.28 0.26 0 0.05 0
Vac/Vac Membrane 0.19 0.15 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0.28 0.24 0.23 0.12 0.19 0.09 0.19 0.13 0.1
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 5 3 2 3 6
Bud 0 0 7 11 5 9 22 26 8 24 8 13 2 0 9 0 0 0 4 2 2 4 9 8
Bud Neck 8 0 34 20 42 38 46 35 11 20 11 18 0 4 5 0 0 0 3 2 0 2 0 4
Bud Site 2 0 4 5 6 10 17 20 12 16 3 9 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 1 2 0 0 0 0 0 2 0 1 0 0 0 1 0 0 0 0 0 2 0 0 1
Cytoplasm 164 91 424 235 294 325 437 508 288 323 205 216 156 156 162 69 44 49 109 55 195 108 148 173
Endoplasmic Reticulum 2 3 1 0 0 0 0 1 0 0 3 0 0 1 0 18 9 11 1 2 3 2 1 3
Endosome 8 14 1 0 3 0 0 0 0 0 0 0 59 46 24 59 24 30 13 6 11 2 3 3
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 1 3 4 0 0 3 0
Mitochondria 2 25 44 50 54 121 218 264 267 262 213 276 2 1 6 10 7 5 25 19 54 6 12 20
Nucleus 3 4 2 6 2 2 3 6 3 2 6 5 3 3 3 37 39 27 14 13 15 8 6 2
Nuclear Periphery 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0 0 1 1 0 0 0 0
Nucleolus 3 1 0 0 1 2 1 3 2 4 2 4 1 0 0 30 15 13 4 1 3 0 0 0
Peroxisomes 0 1 0 0 0 0 0 0 0 1 0 0 3 0 1 0 2 1 1 0 1 0 0 2
SpindlePole 44 20 63 50 92 64 103 107 36 69 16 39 50 35 17 77 54 24 92 66 132 3 11 11
Vac/Vac Membrane 50 25 1 1 2 2 2 6 5 3 4 11 21 8 7 78 45 33 38 44 43 33 30 26
Unique Cell Count 258 168 533 329 441 472 698 771 497 553 381 455 261 233 222 283 186 144 329 235 506 181 241 273
Labelled Cell Count 287 187 583 378 501 573 849 976 634 725 472 591 297 254 235 381 239 194 329 235 506 181 241 273


Microtubule, Nucleus, SpindlePole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.9 3.9 3.0 3.0 3.1 2.8 2.6 2.8 2.3 2.3 2.4 2.2 4.4 4.5 3.9 6.0 6.5 6.4 4.6 4.6 5.0
Std Deviation (1e-4) 0.7 1.0 0.6 1.2 0.8 0.6 0.8 0.8 0.6 0.7 0.6 0.6 1.4 1.1 1.0 1.1 1.7 2.0 1.7 1.7 1.7
Intensity Change (Log2) – – – -0.03 0.04 -0.13 -0.23 -0.1 -0.4 -0.38 -0.34 -0.44 0.55 0.57 0.37 0.99 1.09 1.08 0.62 0.61 0.73


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 12.6 11.5 11.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.208 0.6708 0.5 0.5133 0.4864 0.4306 4.2425 3.7363 3.9975 3.7575 3.6933 3.9298 3.5955 3.7032 3.5398 3.5135 3.2259 3.9099
Actin 0.0524 0.0062 0.0111 0.0008 0.0028 0.0006 0.0135 0.0005 0.0163 0.0019 0.0011 0.0077 0.0059 0.0025 0.0014 0.0009 0.0006 0.0019
Bud 0.0135 0.0281 0.0139 0.0035 0.0518 0.0103 0.0014 0.0065 0.0028 0.0033 0.0021 0.001 0.0034 0.0116 0.002 0.0078 0.0002 0.0004
Bud Neck 0.005 0.0019 0.0072 0.0021 0.0023 0.0057 0.0092 0.0018 0.0015 0.0237 0.0001 0.0024 0.0069 0.0015 0.0033 0.0004 0.0004 0.0065
Bud Periphery 0.0077 0.0068 0.005 0.0012 0.0207 0.0035 0.0008 0.0018 0.0018 0.0023 0.001 0.001 0.0017 0.0023 0.001 0.0029 0.0001 0.0005
Bud Site 0.0183 0.0282 0.0101 0.0004 0.0044 0.0005 0.002 0.0157 0.0046 0.0004 0.0002 0.0005 0.0028 0.0156 0.0083 0.001 0.0002 0.0004
Cell Periphery 0.0009 0.0007 0.0004 0.0001 0.0005 0.0002 0.0001 0.0002 0.0003 0.0001 0.0001 0.0001 0.0001 0.0003 0.0001 0.0001 0.0001 0.0001
Cytoplasm 0.094 0.1649 0.1098 0.1552 0.1422 0.2273 0.0625 0.0793 0.0583 0.0819 0.0661 0.0874 0.0603 0.1035 0.0723 0.0937 0.127 0.1071
Cytoplasmic Foci 0.183 0.062 0.1159 0.0203 0.0283 0.0796 0.0588 0.0344 0.041 0.0112 0.0053 0.0499 0.0726 0.0448 0.0453 0.0184 0.0254 0.0377
Eisosomes 0.0005 0.0005 0.0002 0.0001 0.0001 0 0.0004 0.0001 0.0002 0.0001 0.0002 0.0001 0.0003 0.0002 0.0002 0.0001 0.0003 0.0002
Endoplasmic Reticulum 0.0108 0.0029 0.0043 0.0023 0.0027 0.0023 0.0037 0.0016 0.0019 0.0011 0.0009 0.0014 0.0012 0.002 0.0012 0.0025 0.0016 0.0013
Endosome 0.0307 0.0059 0.0084 0.0057 0.0079 0.0052 0.0191 0.0057 0.0051 0.002 0.0023 0.0047 0.008 0.0052 0.0061 0.004 0.0034 0.0068
Golgi 0.0054 0.0009 0.001 0.0003 0.0005 0.0002 0.0045 0.0004 0.0016 0.0002 0.0006 0.0005 0.0007 0.0006 0.0004 0.0002 0.0002 0.0003
Lipid Particles 0.0275 0.0033 0.0036 0.0007 0.001 0.0024 0.0155 0.0027 0.0033 0.0003 0.0192 0.0013 0.0045 0.0027 0.0024 0.0012 0.0021 0.0025
Mitochondria 0.0098 0.0021 0.0017 0.0013 0.0073 0.0009 0.0017 0.0073 0.0028 0.0009 0.0314 0.0007 0.001 0.0015 0.0008 0.0019 0.0007 0.0006
None 0.3075 0.4341 0.274 0.533 0.4874 0.4066 0.5731 0.6072 0.4797 0.6548 0.7827 0.607 0.6195 0.5348 0.4347 0.6562 0.6951 0.6283
Nuclear Periphery 0.031 0.0056 0.0076 0.0155 0.0219 0.0069 0.0196 0.0041 0.0092 0.0048 0.0146 0.0051 0.0025 0.0067 0.0054 0.0143 0.0048 0.0037
Nucleolus 0.0077 0.0088 0.0076 0.0043 0.0107 0.0055 0.0062 0.0106 0.0084 0.0019 0.0023 0.0024 0.0029 0.0109 0.0075 0.0021 0.0016 0.0024
Nucleus 0.0391 0.0909 0.0989 0.0998 0.1015 0.102 0.0406 0.0593 0.0732 0.0598 0.044 0.0643 0.0359 0.0842 0.0908 0.1024 0.055 0.0647
Peroxisomes 0.0119 0.0011 0.0018 0.0006 0.0011 0.0007 0.0063 0.0016 0.0065 0.0004 0.0032 0.0011 0.0058 0.0105 0.0014 0.0008 0.0009 0.0014
Punctate Nuclear 0.1361 0.1426 0.3149 0.1512 0.099 0.1372 0.1581 0.1569 0.2803 0.1475 0.0207 0.1606 0.1627 0.1557 0.3139 0.0867 0.0794 0.1317
Vacuole 0.0052 0.0023 0.0021 0.0013 0.0047 0.002 0.0019 0.002 0.0009 0.001 0.0008 0.0006 0.0011 0.0026 0.0012 0.0018 0.0009 0.0011
Vacuole Periphery 0.002 0.0003 0.0004 0.0005 0.0012 0.0004 0.0009 0.0002 0.0003 0.0002 0.0011 0.0002 0.0001 0.0004 0.0002 0.0007 0.0002 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.6372 6.1126 3.1034 3.0392 6.9991 8.2728 5.3672 2.9639 1.719 10.8485
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency –
Standard name
Human Ortholog
Description Protein kinase involved in the cell cycle checkpoint into anaphase; in complex with Mad1p and Bub3p, prevents progression into anaphase in presence of spindle damage; Cdc28p-mediated phosphorylation at Bub1p-T566 is important for degradation in anaphase and adaptation of checkpoint to prolonged mitotic arrest; associates with centromere DNA via Skp1p; involved in Sgo1p relocalization in response to sister kinetochore tension; paralog MAD3 arose from whole genome duplication
Localization
Cell Percentages cytoplasm (16%), nucleus (14%), mixed (38%)
Cell Cycle Regulation Yes
nucleus - S/G2 (1.2e-11)
Subcompartmental Group N/A

Bub1

Bub1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Bub1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available