Standard name | |
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Human Ortholog | |
Description | Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp41p (EXOSC4) |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 2 | 3 | 3 | 3 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2 | 4 | 5 | 1 | 3 | 7 | 10 | – | 8 | 7 | 7 | 3 | 12 | 22 | 46 | 17 | 12 | 17 | 0 | 2 | 0 | 3 | 2 | 3 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 4 | 9 | 0 | 0 | 0 | 0 | 0 | 1 |
Endosome | 1 | 1 | 1 | 0 | 0 | 0 | 2 | – | 0 | 0 | 2 | 0 | 2 | 0 | 4 | 1 | 3 | 5 | 0 | 3 | 2 | 3 | 0 | 3 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 1 | 1 | 0 |
Mitochondria | 1 | 15 | 1 | 0 | 0 | 0 | 3 | – | 18 | 17 | 8 | 10 | 0 | 0 | 0 | 11 | 10 | 6 | 0 | 1 | 0 | 0 | 0 | 5 |
Nucleus | 120 | 138 | 149 | 186 | 250 | 207 | 244 | – | 213 | 265 | 211 | 257 | 177 | 257 | 260 | 161 | 132 | 188 | 92 | 116 | 135 | 193 | 193 | 134 |
Nuclear Periphery | 0 | 0 | 2 | 1 | 6 | 1 | 2 | – | 1 | 1 | 0 | 5 | 0 | 0 | 1 | 3 | 1 | 1 | 0 | 0 | 1 | 1 | 2 | 5 |
Nucleolus | 104 | 76 | 85 | 13 | 3 | 13 | 16 | – | 46 | 32 | 37 | 60 | 72 | 52 | 48 | 61 | 52 | 49 | 114 | 93 | 123 | 53 | 71 | 86 |
Peroxisomes | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 1 | 1 | 0 | – | 1 | 1 | 1 | 1 | 1 | 2 | 4 | 0 | 0 | 1 | 1 | 1 | 1 | 0 | 4 | 1 |
Vac/Vac Membrane | 7 | 3 | 4 | 1 | 1 | 7 | 6 | – | 3 | 5 | 9 | 4 | 3 | 7 | 11 | 14 | 15 | 26 | 10 | 8 | 4 | 13 | 56 | 94 |
Unique Cell Count | 206 | 211 | 227 | 196 | 257 | 230 | 273 | 260 | 305 | 257 | 311 | 245 | 313 | 352 | 236 | 211 | 274 | 226 | 233 | 275 | 274 | 339 | 346 | |
Labelled Cell Count | 236 | 237 | 247 | 204 | 264 | 236 | 283 | 293 | 330 | 277 | 340 | 268 | 340 | 377 | 270 | 229 | 304 | 226 | 233 | 275 | 274 | 339 | 346 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 11.0 | 9.5 | 12.7 | 12.6 | 11.1 | 9.8 | 9.2 | – | 8.2 | 8.3 | 8.9 | 8.7 | 13.6 | 12.5 | 11.9 | 10.9 | 14.2 | 14.1 | 13.7 | 13.5 | 13.4 |
Std Deviation (1e-4) | 2.7 | 2.0 | 2.9 | 2.4 | 2.4 | 2.1 | 1.9 | – | 1.9 | 2.0 | 2.1 | 2.0 | 3.4 | 3.4 | 3.7 | 3.2 | 4.6 | 4.5 | 3.2 | 2.7 | 2.8 |
Intensity Change (Log2) | – | – | – | -0.01 | -0.2 | -0.37 | -0.47 | – | -0.64 | -0.61 | -0.51 | -0.55 | 0.1 | -0.03 | -0.1 | -0.23 | 0.16 | 0.15 | 0.11 | 0.09 | 0.08 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 0.6 | 1.0 | – | 0.6 | 0.1 | 0.4 | 0 | 1.6 | 2.5 | 4.5 | 2.5 | 1.9 | 2.2 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | – | 3.7 | 3.2 | 0 | 0 | 0 | 0 | 0 | 2.9 | 2.9 | 0 |
Nucleus | 7.4 | 9.1 | 6.3 | 6.4 | – | 4.1 | 5.8 | 4.1 | 4.5 | 1.6 | 4.4 | 2.1 | 0.6 | -0.7 | 0.7 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | -7.5 | -10.3 | -8.3 | -8.8 | – | -4.9 | -7.4 | -5.8 | -4.7 | -1.9 | -5.5 | -6.6 | -2.7 | -2.9 | -4.9 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0.9 | 0 | – | 0 | 0 | 1.2 | 0 | 0 | 0 | 1.0 | 2.3 | 2.7 | 3.6 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 8.9038 | 8.2944 | 7.829 | 6.9196 | 6.2197 | 7.9171 | 10.4508 | 11.6365 | 11.665 | 10.6424 | 11.0061 | 11.348 | 7.9769 | 9.7598 | 9.3119 | 8.556 | 7.5994 | 8.8657 |
Actin | 0.0068 | 0.0033 | 0.0081 | 0.0026 | 0.0227 | 0.0006 | 0.0095 | 0.0004 | 0.0045 | 0 | 0 | 0.0028 | 0.0412 | 0.0036 | 0.0007 | 0.0735 | 0.013 | 0 |
Bud | 0.0003 | 0.0001 | 0.0005 | 0.0001 | 0.0002 | 0.0001 | 0.0007 | 0.0002 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0006 | 0.0003 | 0 | 0.0004 | 0.0003 | 0.0001 |
Bud Neck | 0.0006 | 0.0003 | 0.0017 | 0.0002 | 0.0007 | 0.0007 | 0.0008 | 0.0004 | 0.0004 | 0.0003 | 0.0003 | 0.0007 | 0.0012 | 0.0003 | 0.0002 | 0.0003 | 0.0006 | 0.0006 |
Bud Periphery | 0.0005 | 0.0001 | 0.001 | 0.0001 | 0.0005 | 0.0002 | 0.0016 | 0.0003 | 0.0003 | 0.0001 | 0.0001 | 0.0002 | 0.0009 | 0.0004 | 0.0001 | 0.0004 | 0.0004 | 0.0001 |
Bud Site | 0.0012 | 0.0009 | 0.0074 | 0.0001 | 0.0005 | 0.0001 | 0.0016 | 0.0005 | 0.0007 | 0.0002 | 0.0001 | 0.0002 | 0.0043 | 0.0055 | 0.0002 | 0.0007 | 0.0009 | 0.0001 |
Cell Periphery | 0.0004 | 0.0002 | 0.0004 | 0 | 0.0002 | 0.0001 | 0.0005 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0003 | 0.0003 | 0.0001 | 0.0001 | 0.0002 | 0.0001 |
Cytoplasm | 0.0097 | 0.0053 | 0.0019 | 0.0003 | 0.0031 | 0.0003 | 0.01 | 0.0006 | 0.0058 | 0.0001 | 0 | 0.0004 | 0.0059 | 0.0001 | 0.0011 | 0.0002 | 0.0056 | 0 |
Cytoplasmic Foci | 0.0183 | 0.0006 | 0.0027 | 0.0088 | 0.0039 | 0.0052 | 0.0225 | 0.0011 | 0.0067 | 0.0181 | 0 | 0.0019 | 0.0103 | 0.0001 | 0.0095 | 0.0009 | 0.0023 | 0 |
Eisosomes | 0.0003 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0.0004 | 0 | 0.0001 | 0 | 0 | 0 | 0.0005 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0034 | 0.0006 | 0.0005 | 0.0001 | 0.0136 | 0 | 0.0073 | 0.0002 | 0.0052 | 0 | 0 | 0.0002 | 0.0028 | 0 | 0.0002 | 0.0003 | 0.0095 | 0 |
Endosome | 0.0114 | 0.0005 | 0.0034 | 0.0026 | 0.0254 | 0.0012 | 0.0125 | 0.0024 | 0.0108 | 0.0003 | 0 | 0.0043 | 0.01 | 0.0002 | 0.0025 | 0.0013 | 0.0115 | 0 |
Golgi | 0.0029 | 0.0012 | 0.0021 | 0.0021 | 0.0051 | 0.0004 | 0.003 | 0.0008 | 0.0042 | 0.0003 | 0 | 0.0037 | 0.0154 | 0.0002 | 0.0005 | 0.0024 | 0.0018 | 0 |
Lipid Particles | 0.0055 | 0.0001 | 0.0021 | 0.0359 | 0.0094 | 0.0013 | 0.0043 | 0.0007 | 0.0018 | 0.0245 | 0 | 0.0023 | 0.0126 | 0.0002 | 0.0012 | 0.0005 | 0.0019 | 0.0001 |
Mitochondria | 0.0084 | 0.0004 | 0.0134 | 0.0048 | 0.0056 | 0.0002 | 0.0073 | 0.0009 | 0.0078 | 0.0002 | 0.0002 | 0.0015 | 0.0283 | 0.0028 | 0.0002 | 0.0014 | 0.0022 | 0.0001 |
None | 0.0066 | 0.0003 | 0.0019 | 0.0006 | 0.0017 | 0.0002 | 0.0168 | 0.0001 | 0.0056 | 0 | 0 | 0.0002 | 0.0015 | 0.0001 | 0.0021 | 0.0002 | 0.0053 | 0 |
Nuclear Periphery | 0.0125 | 0.0008 | 0.0078 | 0.0013 | 0.0389 | 0.0009 | 0.0346 | 0.0028 | 0.0018 | 0.0004 | 0.0008 | 0.0022 | 0.0122 | 0.0008 | 0.0006 | 0.0005 | 0.0134 | 0.0002 |
Nucleolus | 0.3299 | 0.2673 | 0.375 | 0.2752 | 0.2398 | 0.4227 | 0.3625 | 0.3787 | 0.4596 | 0.3754 | 0.2679 | 0.4661 | 0.2987 | 0.3366 | 0.5059 | 0.4252 | 0.3353 | 0.4396 |
Nucleus | 0.5651 | 0.7164 | 0.5499 | 0.6509 | 0.6057 | 0.554 | 0.4617 | 0.6004 | 0.4712 | 0.5771 | 0.7295 | 0.5062 | 0.5309 | 0.6441 | 0.466 | 0.4901 | 0.5905 | 0.558 |
Peroxisomes | 0.0026 | 0.0001 | 0.0046 | 0.0114 | 0.0026 | 0.0032 | 0.0086 | 0.0002 | 0.0006 | 0.0005 | 0 | 0.0006 | 0.0148 | 0.0005 | 0.0052 | 0.0008 | 0.0006 | 0 |
Punctate Nuclear | 0.008 | 0.0006 | 0.0101 | 0.0021 | 0.0129 | 0.0081 | 0.0251 | 0.0071 | 0.009 | 0.002 | 0.0005 | 0.0056 | 0.0029 | 0.0003 | 0.0036 | 0.0005 | 0.0028 | 0.0008 |
Vacuole | 0.0031 | 0.0006 | 0.003 | 0.0002 | 0.0041 | 0.0004 | 0.0046 | 0.0015 | 0.0028 | 0.0003 | 0.0003 | 0.0004 | 0.0028 | 0.0022 | 0.0002 | 0.0002 | 0.0009 | 0.0001 |
Vacuole Periphery | 0.0025 | 0.0004 | 0.0022 | 0.0005 | 0.0031 | 0.0002 | 0.0039 | 0.0005 | 0.0008 | 0.0001 | 0.0001 | 0.0002 | 0.002 | 0.0013 | 0.0001 | 0.0002 | 0.001 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 46.9059 | 55.908 | 46.4249 | 39.3314 | 41.509 | 29.765 | 38.6951 | 33.1567 | 32.7183 | 41.6417 |
Translational Efficiency | 1.6439 | 1.3717 | 1.4006 | 1.4725 | 1.4544 | 1.9999 | 1.4147 | 1.6576 | 1.5181 | 1.5039 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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