Standard name
Human Ortholog
Description ATP-binding protein of unknown function; crystal structure resembles that of E.coli pantothenate kinase and other small kinases; null mutant is sensitive to expression of the top1-T722A allele
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.97 0.98 0.96 0.95 0.88 0.81 0.78 0.72 0.76 0.7 0.68 1.0 1.0 0.98 0.96 0.94 0.93 0.96 0.94 0.93 0.77 0.77 0.72
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.05 0.06 0.06 0 0.24 0.1 0.22 0.15 0 0 0 0 0 0 0 0 0 0.11 0.11 0.13
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0.09 0.16 0.15 0.11 0.12 0.11 0.16 0 0 0 0.05 0.1 0.14 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 0 0 3 3 0 0 0 0 0 0 0 5 1 2 1 6
Bud 0 2 0 0 5 1 2 1 4 2 7 7 0 0 0 0 0 0 0 0 2 3 3 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 0 0 2
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 2 3 2 0 3 5 6 10 10 18 20 0 2 0 8 8 2 0 0 0 0 0 1
Cytoplasm 489 526 388 317 212 394 481 499 298 414 393 392 215 212 236 576 423 425 487 519 365 206 213 233
Endoplasmic Reticulum 2 5 2 2 0 4 2 18 4 12 7 10 0 1 1 16 11 10 0 1 1 3 2 7
Endosome 0 0 1 0 1 3 0 0 1 0 0 0 0 0 2 2 2 1 4 6 4 5 3 7
Golgi 1 1 2 0 0 0 1 0 0 0 0 0 0 0 0 1 1 1 1 1 2 0 1 1
Mitochondria 4 10 1 6 12 26 37 28 100 56 124 88 0 0 1 10 6 1 0 6 3 28 29 43
Nucleus 7 2 0 4 2 8 14 26 12 16 20 16 2 1 0 4 7 3 1 2 1 2 1 0
Nuclear Periphery 1 2 0 0 0 0 0 0 0 3 1 3 0 0 0 0 0 0 0 0 0 1 2 3
Nucleolus 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 3 1
SpindlePole 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 1 2 1 4
Vac/Vac Membrane 0 6 2 13 9 39 96 93 45 64 59 91 1 0 1 28 47 64 4 3 3 3 4 4
Unique Cell Count 501 542 394 331 224 447 594 638 416 545 560 574 216 213 240 598 451 455 510 556 394 268 276 326
Labelled Cell Count 506 556 399 346 241 478 638 672 474 577 632 630 218 216 241 645 505 508 510 556 394 268 276 326


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.7 6.2 7.2 6.8 5.9 6.7 7.0 7.5 6.7 7.6 7.3 7.6 7.1 7.0 7.1 8.5 10.3 10.3 7.4 7.6 7.7
Std Deviation (1e-4) 1.1 0.9 1.4 1.8 1.3 1.3 1.4 1.6 1.6 1.7 1.9 1.9 1.1 0.9 1.3 1.7 1.7 2.0 2.1 1.7 1.7
Intensity Change (Log2) – – – -0.07 -0.28 -0.09 -0.03 0.07 -0.1 0.09 0.03 0.09 -0.01 -0.04 -0.02 0.24 0.52 0.52 0.05 0.08 0.1


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 1.9 1.4 2.5 2.7 0 0 0 0 1.3 0
Cytoplasm -2.2 -2.7 -5.9 -8.3 -9.1 -10.6 -9.6 -11.2 -11.7 1.2 1.2 -0.1 -2.0 -3.5 -3.7
Endoplasmic Reticulum 0 0 0 0 2.6 0 2.1 0 0 0 0 0 2.5 2.3 2.1
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 4.6 4.8 3.9 10.2 6.3 9.9 8.0 0 0 0 0 0 0
Nucleus 0 0 0 3.1 4.1 3.4 3.4 3.8 3.3 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 3.2 0 5.5 8.1 7.6 6.3 6.6 6.2 8.0 0 0 0 3.8 6.2 7.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.04 4.095 3.1546 3.0957 2.5043 3.2337 5.8375 6.8256 6.8866 5.1404 6.2609 6.8568 3.9292 4.5547 4.334 4.0564 3.9572 4.3042
Actin 0.0358 0.0042 0.0069 0.0382 0.0432 0.0007 0.0011 0.006 0.0005 0.0013 0.0005 0.0038 0.0205 0.0018 0.0033 0.0097 0.0013 0.0028
Bud 0.0006 0.0017 0.0005 0.0004 0.0001 0.0001 0.0002 0.0002 0.0028 0.0011 0.0006 0.012 0.0005 0.0058 0.0017 0.0004 0.0001 0.0001
Bud Neck 0.0015 0.0001 0.0004 0.0003 0.0004 0.0011 0.0002 0.0001 0.0002 0.0004 0.0004 0.0064 0.0027 0.002 0.0005 0.0047 0.0002 0.0013
Bud Periphery 0.001 0.002 0.0006 0.0007 0.0002 0 0.0001 0.0001 0.0016 0.0006 0.0002 0.004 0.0007 0.0044 0.0013 0.0003 0.0001 0.0001
Bud Site 0.0026 0.001 0.0028 0.0005 0.0003 0.0001 0.0003 0.0006 0.0006 0.0003 0.0003 0.0005 0.0029 0.0114 0.0048 0.0044 0.0001 0.0002
Cell Periphery 0.0004 0.0002 0.0002 0 0.0001 0 0.0001 0.0001 0.0001 0.0001 0 0.0001 0.0002 0.0003 0.0002 0.0002 0 0
Cytoplasm 0.587 0.7838 0.6988 0.7168 0.6854 0.8217 0.8334 0.827 0.8135 0.9038 0.7941 0.7745 0.6077 0.7394 0.6798 0.6783 0.636 0.6967
Cytoplasmic Foci 0.0177 0.0028 0.01 0.0176 0.0177 0.0108 0.0037 0.0158 0.0049 0.0093 0.005 0.0054 0.0125 0.0073 0.0073 0.0076 0.0076 0.0111
Eisosomes 0.0004 0.0001 0.0001 0.0001 0.0005 0 0 0.0001 0 0 0 0 0.0002 0 0 0.0001 0.0002 0
Endoplasmic Reticulum 0.0048 0.0051 0.0023 0.0013 0.0013 0.002 0.0043 0.0014 0.0026 0.0028 0.0031 0.0033 0.0025 0.0014 0.0023 0.003 0.0012 0.0017
Endosome 0.0127 0.0012 0.0071 0.0017 0.0072 0.0017 0.0012 0.0017 0.0008 0.0046 0.0021 0.0036 0.0133 0.0015 0.0028 0.0025 0.0021 0.0035
Golgi 0.0062 0.0003 0.0019 0.0012 0.0059 0.0002 0.0001 0.001 0.0001 0.0003 0.0001 0.0002 0.002 0.0002 0.0009 0.0008 0.0003 0.0005
Lipid Particles 0.0082 0.0002 0.0051 0.0026 0.0191 0.0001 0.0001 0.0007 0.0001 0.0001 0 0.0001 0.0027 0.0001 0.001 0.0002 0.0019 0.0009
Mitochondria 0.0055 0.0003 0.0125 0.0006 0.0019 0.0001 0.0002 0.0002 0.0009 0.0002 0.0002 0.0003 0.0019 0.0003 0.0007 0.0003 0.0003 0.0003
None 0.2839 0.1929 0.2374 0.1755 0.1866 0.1575 0.1488 0.138 0.1579 0.0626 0.1824 0.1809 0.3133 0.2117 0.2788 0.2804 0.3389 0.2701
Nuclear Periphery 0.0016 0.0007 0.001 0.0004 0.0016 0.0005 0.0014 0.0004 0.0014 0.0016 0.002 0.0007 0.0029 0.001 0.0019 0.001 0.0022 0.0008
Nucleolus 0.0013 0.0001 0.0003 0 0.0003 0 0 0 0.0008 0 0 0 0.0006 0.0003 0.0002 0.0001 0.0004 0.0001
Nucleus 0.003 0.0022 0.002 0.0025 0.0014 0.0021 0.0031 0.002 0.0053 0.0078 0.007 0.0029 0.0041 0.0063 0.0054 0.0036 0.002 0.0037
Peroxisomes 0.0195 0.0002 0.0071 0.0383 0.0157 0.0003 0.0001 0.0036 0.0002 0.0001 0.0001 0.0001 0.0033 0.0005 0.0028 0.0004 0.0004 0.002
Punctate Nuclear 0.0034 0.0003 0.0011 0.0008 0.0106 0.0009 0.0008 0.0004 0.0051 0.0008 0.0009 0.0005 0.0038 0.0037 0.0033 0.0015 0.0043 0.0035
Vacuole 0.0019 0.0005 0.0014 0.0004 0.0003 0.0003 0.0007 0.0004 0.0005 0.0018 0.0008 0.0005 0.0014 0.0008 0.0009 0.0006 0.0004 0.0004
Vacuole Periphery 0.001 0.0001 0.0004 0.0001 0.0002 0.0001 0.0001 0.0001 0.0001 0.0003 0.0002 0.0001 0.0004 0.0001 0.0002 0.0001 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 10.1114 10.1688 11.7378 16.3515 11.4711 10.1058 15.4225 17.7341 20.1241 17.7399
Translational Efficiency 1.4234 0.8742 1.4673 1.4151 1.4564 2.4068 1.3408 1.218 1.4142 1.3156

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description ATP-binding protein of unknown function; crystal structure resembles that of E.coli pantothenate kinase and other small kinases; null mutant is sensitive to expression of the top1-T722A allele
Localization
Cell Percentages cytoplasm (93%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Tda10

Tda10


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tda10-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available