Standard name
Human Ortholog
Description Mitochondrial protein that interacts with frataxin (Yfh1p); putative ortholog of mammalian electron transfer flavoprotein complex subunit ETF-beta; may have a role in oxidative stress response
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0.05 0 0 0.11 0.12 0.06 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 – 0 0 0.1 0 0 0 0 0.14 0.06 0.12 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0.05 0.34 0.19 0.38 0.05 0.06 – 0 0 0 0 0.18 0.19 0.35 0.09 0.24 0.33 0.05 0.07 0.05 0.08 0.1 0.09
Mitochondria 0.93 0.95 0.76 0.89 0.8 0.78 0.83 – 0.87 0.85 0.79 0.91 0.84 0.9 0.78 0.08 0.06 0.12 0.87 0.85 0.83 0.79 0.72 0.74
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.07 0 0 0 0 0.06 0 – 0.07 0.06 0.07 0.06 0.06 0.05 0 0.1 0.06 0.07 0 0 0 0 0 0
Nucleolus 0.21 0 0 0 0.09 0.15 0.11 – 0.14 0.15 0.15 0.1 0 0 0 0.14 0.1 0.12 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0.05 0 0 0.2 0.16 0.12 0 0 0.06 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.14 0.07 0 0.08 0.06 0.05 – 0 0 0 0 0.06 0.05 0.09 0.19 0.19 0.16 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 1 8 4 5 3 2 – 2 2 0 1 6 15 5 1 3 8 1 2 6 5 11 14
Bud 0 0 1 0 2 1 4 – 3 4 2 5 1 1 3 5 1 1 0 0 0 0 1 2
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 1 1 0 2 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 8 11 11 4 0 2 – 3 3 0 0 10 6 4 14 12 11 0 0 1 0 0 0
Cytoplasm 1 0 7 8 3 7 5 – 13 2 15 3 3 6 4 18 6 22 0 0 0 1 1 1
Endoplasmic Reticulum 0 0 1 2 0 4 3 – 2 6 1 3 1 0 4 4 5 3 0 0 0 0 1 0
Endosome 0 0 3 3 1 3 2 – 1 4 0 3 1 3 1 4 3 7 0 1 4 2 5 6
Golgi 0 10 109 50 111 13 21 – 2 5 2 8 34 60 94 12 25 62 4 16 18 14 30 30
Mitochondria 80 210 244 228 235 186 301 – 278 341 120 253 162 284 209 10 6 23 86 188 306 147 211 253
Nucleus 1 0 3 0 0 2 0 – 2 3 2 0 0 0 1 4 1 2 0 0 0 0 0 0
Nuclear Periphery 6 5 12 2 4 14 13 – 24 23 10 17 12 15 7 13 6 14 0 0 0 0 2 3
Nucleolus 18 9 13 6 26 35 41 – 46 62 23 29 8 6 1 19 10 22 1 0 1 0 4 1
Peroxisomes 1 1 7 8 3 2 5 – 2 7 0 2 9 7 3 27 16 22 1 2 21 4 7 10
SpindlePole 0 0 1 1 2 0 3 – 1 5 0 1 0 0 0 2 2 2 0 0 0 1 1 3
Vac/Vac Membrane 1 31 21 6 23 15 19 – 7 16 4 9 12 16 24 25 20 30 0 7 2 4 12 10
Unique Cell Count 86 221 320 257 294 238 361 321 401 151 278 192 315 267 133 103 190 99 221 367 187 293 344
Labelled Cell Count 109 275 441 329 419 285 421 387 484 179 336 259 419 360 158 116 229 99 221 367 187 293 344


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 15.0 15.3 20.6 19.6 21.2 20.0 18.2 – 16.3 16.3 14.7 16.4 19.1 17.8 17.8 13.4 17.8 16.4 19.9 21.2 21.6
Std Deviation (1e-4) 4.3 3.5 5.4 4.2 4.5 5.6 4.8 – 4.6 5.2 4.7 5.0 4.2 3.6 3.8 4.7 6.2 5.6 5.2 5.3 5.5
Intensity Change (Log2) – – – -0.08 0.04 -0.04 -0.18 – -0.34 -0.34 -0.49 -0.33 -0.11 -0.21 -0.21 -0.62 -0.21 -0.33 -0.05 0.04 0.07


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -0.8 -0.7 0 0 – 0 0 0 0 0.4 1.5 -0.5 0 0 1.1
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.5 -1.7 0 -2.7 – -2.2 -2.6 0 -3.1 1.0 -1.2 -1.5 3.0 3.2 1.3
Cytoplasm 0.7 0 0.6 -0.8 – 1.4 0 3.7 0 0 -0.3 -0.6 4.8 0 4.4
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi -3.9 1.0 -8.1 -9.4 – -11.2 -12.0 -7.8 -9.6 -4.0 -4.3 0.3 -5.5 -1.9 -0.3
Mitochondria 3.9 1.1 0.5 2.3 – 3.4 3.0 0.8 4.8 2.2 4.7 0.6 -13.4 -12.6 -14.0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery -2.3 -1.9 1.2 -0.1 – 2.0 1.2 1.4 1.3 1.3 0.6 -0.8 2.6 0 1.8
Nucleolus -1.2 2.4 4.4 3.5 – 4.5 5.0 4.3 3.0 0.1 -1.6 -2.9 3.9 2.2 3.2
Peroxisomes 0.7 0 0 -0.8 – 0 -0.4 0 0 1.6 0 0 6.7 5.2 4.4
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole -2.4 0.6 -0.1 -0.7 – -2.7 -1.6 -1.8 -1.9 -0.1 -0.8 1.1 3.9 3.8 3.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 24.0308 23.2684 23.7635 23.6817 23.3405 24.4628 26.7853 28.6613 28.3506 27.8699 23.7716 28.5159 26.481 25.7886 25.713 24.2165 24.32 25.9177
Actin 0.0036 0.0037 0.0017 0.0001 0.0001 0.0003 0.018 0.0016 0.0033 0.0003 0.0074 0.0034 0.0002 0.0049 0.0015 0.0001 0.0005 0.0003
Bud 0 0.0002 0.0001 0 0 0 0.0002 0.0001 0.0001 0.0001 0.0001 0 0 0.0002 0.0003 0.0001 0 0
Bud Neck 0.0005 0.0001 0.0001 0 0 0 0.0004 0 0.0003 0 0 0.0002 0 0.0001 0.0001 0 0 0
Bud Periphery 0.0001 0.0004 0.0002 0 0 0 0.0004 0.0002 0.0003 0.0001 0.0002 0.0001 0 0.0007 0.0004 0.0001 0.0001 0
Bud Site 0.0016 0.0004 0.0012 0 0 0 0.0024 0.0001 0.0026 0.0002 0.0001 0.0001 0 0.0003 0.0013 0 0 0
Cell Periphery 0.0001 0.0005 0.0002 0 0 0 0.0004 0.0005 0.0004 0.0001 0.0002 0.0001 0.0001 0.0005 0.0002 0.0001 0 0.0001
Cytoplasm 0.0025 0 0 0 0 0 0.0019 0 0.0012 0.0001 0.0001 0 0 0 0 0 0 0
Cytoplasmic Foci 0.0056 0.0003 0.0003 0 0 0.0001 0.0105 0.0003 0.0042 0.0011 0.0029 0.0002 0.0002 0.0002 0.0001 0.0001 0 0.0001
Eisosomes 0.0008 0.0003 0.0003 0.0001 0.0001 0.0001 0.0006 0.0004 0.0001 0.0001 0.0006 0.0002 0.0001 0.0003 0.0004 0.0001 0.0001 0.0002
Endoplasmic Reticulum 0.0023 0 0 0 0 0 0.0011 0.0003 0.0006 0.0002 0.0006 0.0002 0 0.0001 0 0 0 0.0001
Endosome 0.0022 0.0013 0.0004 0.0001 0 0.0006 0.0038 0.0012 0.0019 0.001 0.004 0.001 0.0005 0.0006 0.0002 0.0005 0.0001 0.0005
Golgi 0.004 0.0056 0.0007 0.0003 0.0001 0.0067 0.0023 0.0055 0.0017 0.0006 0.0059 0.0019 0.001 0.0011 0.0004 0.0016 0.0002 0.0053
Lipid Particles 0.0094 0.0048 0.0014 0.002 0.0004 0.0015 0.0224 0.0087 0.0074 0.0096 0.0361 0.0084 0.0059 0.0053 0.0116 0.0031 0.0014 0.0088
Mitochondria 0.9432 0.9627 0.9826 0.9951 0.9985 0.9871 0.9097 0.9432 0.9565 0.9624 0.8905 0.976 0.982 0.9652 0.9666 0.9823 0.9932 0.9707
None 0.0023 0 0 0 0 0 0.0019 0 0.0009 0.0003 0.0008 0.0001 0 0 0 0 0 0
Nuclear Periphery 0.0016 0.0001 0 0 0 0 0.0007 0.0001 0.0049 0.0037 0.0088 0.0009 0.0001 0.0002 0 0 0 0.0001
Nucleolus 0.0015 0.0005 0.0001 0 0 0 0.0071 0.0003 0.0002 0.0023 0.0004 0.0001 0.0003 0.0004 0.0005 0.0001 0 0.001
Nucleus 0.0014 0.0002 0.0001 0 0 0 0.0009 0.0001 0.0007 0.001 0.0003 0.0002 0.0001 0.0001 0.0001 0 0 0.0001
Peroxisomes 0.0037 0.0015 0.0017 0.0007 0.0004 0.0003 0.0047 0.0023 0.0051 0.0006 0.0205 0.0011 0.0018 0.0009 0.0008 0.0009 0.0006 0.0007
Punctate Nuclear 0.0068 0 0 0 0 0 0.0016 0 0.0012 0.0025 0.0004 0.0002 0 0 0 0 0 0
Vacuole 0.0008 0.0049 0.0012 0.0001 0 0.0002 0.0028 0.006 0.0013 0.0013 0.0014 0.0004 0.0011 0.0032 0.0016 0.0016 0.0002 0.0006
Vacuole Periphery 0.0059 0.0124 0.0078 0.0015 0.0002 0.003 0.0061 0.0289 0.0051 0.0124 0.0187 0.0053 0.0065 0.0158 0.0139 0.009 0.0034 0.0114

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 49.8357 61.4916 49.3543 54.169 41.8627 60.3309 70.4217 68.5116 86.277 65.4542
Translational Efficiency 2.3019 1.9937 2.1199 2.6109 2.432 2.6127 2.2074 2.2531 2.0015 2.1833

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Mitochondrial protein that interacts with frataxin (Yfh1p); putative ortholog of mammalian electron transfer flavoprotein complex subunit ETF-beta; may have a role in oxidative stress response
Localization
Cell Percentages mitochondrion (100%)
Cell Cycle Regulation No
Subcompartmental Group mito-3

Cir1

Cir1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cir1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available