ORF
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0.05 0.06 0 0 0 0 0.07 0.06 0 0.07 0 0.05 0 0.11 0.17 0.07 0 0 0 0 0 0
Cytoplasm 0.99 0.96 0.97 0.88 0.89 0.83 0.74 0.71 0.69 0.64 0.58 0.59 0.99 1.0 0.99 0.96 0.92 0.96 0.96 0.95 0.93 0.95 0.93 0.94
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.14 0.17 0.29 0.38 0.36 0.34 0.42 0.48 0.48 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.05 0.09 0.07 0.06 0.06 0 0 0 0 0.08 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 0 0 1 1 1 1 0 0 0 0 0 1 1 0 2 0 0 0
Bud 1 1 0 1 5 3 4 5 9 10 9 14 1 0 1 0 0 0 0 0 0 2 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 19 12 7 9 15 18 32 27 12 29 13 24 20 17 38 13 0 0 1 0 0 0
Cytoplasm 135 111 355 177 224 365 350 362 324 287 226 235 390 451 488 146 208 170 133 132 337 265 327 318
Endoplasmic Reticulum 0 2 1 1 1 3 8 8 8 7 11 12 1 2 1 9 6 5 0 1 4 0 1 2
Endosome 0 0 2 1 1 0 0 1 0 2 0 0 0 0 2 0 0 0 0 1 2 0 3 2
Golgi 0 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0 2 1 0 0 1 0 1 0
Mitochondria 2 1 3 28 42 128 179 183 162 188 189 193 0 0 0 1 0 2 0 0 2 2 2 3
Nucleus 1 3 1 0 0 0 2 2 0 1 1 2 2 0 1 0 2 1 1 0 0 1 2 2
Nuclear Periphery 0 0 0 0 0 1 2 0 1 0 0 8 0 0 0 0 0 0 0 0 0 1 1 0
Nucleolus 0 0 1 2 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 1 0 6 4 6 7 13 27 41 33 23 25 0 0 0 2 18 3 0 0 1 2 2 3
Unique Cell Count 137 116 367 202 253 438 475 511 472 448 392 401 392 453 493 152 227 177 139 139 363 280 352 338
Labelled Cell Count 140 118 389 227 286 516 573 606 578 557 473 519 407 478 514 175 274 196 139 139 363 280 352 338


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.2 11.1 9.6 9.3 8.1 7.1 6.8 6.9 6.8 6.8 6.2 6.2 11.6 11.3 10.4 16.8 16.3 16.1 10.8 11.0 12.0
Std Deviation (1e-4) 1.2 1.8 1.5 2.2 1.6 1.3 1.4 1.4 1.4 2.1 1.4 1.3 1.6 1.5 1.4 3.8 2.6 2.9 1.6 1.8 2.1
Intensity Change (Log2) – – – -0.05 -0.24 -0.44 -0.51 -0.48 -0.5 -0.51 -0.65 -0.64 0.26 0.22 0.11 0.8 0.76 0.74 0.17 0.19 0.32


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 3.6 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.4 -1.5 -2.4 -1.5 -1.2 1.0 0.5 -1.5 1.2 -1.3 0.1 -0.8 2.5 4.6 1.0
Cytoplasm -4.2 -4.0 -6.2 -9.0 -9.8 -10.3 -11.3 -12.7 -12.5 2.8 3.1 2.4 -0.4 -2.7 -0.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 2.8 2.9 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 6.6 7.4 10.9 12.9 12.5 12.1 13.8 15.0 15.0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 1.1 2.8 4.4 3.8 3.0 3.2 0 0 0 0 3.8 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.163 5.9098 4.8677 4.437 4.3346 5.0736 3.8675 5.9358 5.0572 4.884 5.0713 5.4879 3.9944 5.8056 4.7065 4.9122 4.5229 4.8713
Actin 0.0026 0.0058 0.0082 0.0042 0.0022 0.0061 0.0189 0.0004 0.004 0.0006 0.0136 0.0032 0.0124 0.0069 0.012 0.0012 0.0004 0.0021
Bud 0.0002 0.0003 0.0003 0.0004 0.0003 0.0002 0.0017 0.0048 0.001 0.0002 0.0004 0.0003 0.0001 0.0014 0.0005 0.0003 0.0004 0.0015
Bud Neck 0.001 0.0006 0.0003 0.0022 0.0004 0.0009 0.0031 0.0001 0.0003 0.0002 0.0004 0.0099 0.0007 0.0009 0.0029 0.0004 0.0005 0.0011
Bud Periphery 0.0001 0.0001 0.0002 0.0002 0.0001 0.0001 0.0013 0.0004 0.0008 0 0.0003 0.0002 0.0001 0.0004 0.0005 0.0001 0.0001 0.0007
Bud Site 0.0003 0.0054 0.0007 0.0005 0.0012 0.0001 0.0136 0.0048 0.0018 0.0001 0.0005 0.0052 0.0006 0.0031 0.0106 0.0004 0.0001 0.0002
Cell Periphery 0.0001 0.0002 0.0002 0.0001 0.0001 0 0.0004 0.0001 0.0001 0.0001 0.0001 0.0001 0.0003 0.0002 0.0004 0.0001 0 0
Cytoplasm 0.9195 0.9597 0.9139 0.7898 0.9152 0.9371 0.6689 0.9653 0.9006 0.9008 0.7812 0.902 0.8924 0.9583 0.9361 0.8876 0.9515 0.9559
Cytoplasmic Foci 0.0151 0.0078 0.0153 0.0774 0.0177 0.0112 0.079 0.0077 0.0227 0.0279 0.0205 0.0105 0.0132 0.0063 0.0061 0.0473 0.0175 0.0145
Eisosomes 0 0.0001 0 0 0 0 0.0001 0 0 0 0.0001 0 0.0017 0 0.0004 0 0 0
Endoplasmic Reticulum 0.0124 0.0015 0.006 0.0026 0.0047 0.0076 0.0198 0.002 0.0029 0.0063 0.0019 0.0024 0.0168 0.0022 0.0047 0.004 0.002 0.0052
Endosome 0.017 0.0041 0.0186 0.0764 0.034 0.014 0.0708 0.0049 0.0202 0.053 0.0862 0.0171 0.0082 0.0039 0.0065 0.0176 0.0193 0.0054
Golgi 0.0015 0.0006 0.0014 0.0094 0.0026 0.0028 0.0242 0.0003 0.0098 0.0024 0.0583 0.0216 0.0014 0.0009 0.0009 0.0013 0.0005 0.0006
Lipid Particles 0.0004 0.0002 0.0004 0.0116 0.0029 0.0004 0.0111 0 0.0066 0.0004 0.0055 0.0128 0.0056 0.0001 0.0009 0.0004 0 0.0001
Mitochondria 0.0008 0.0002 0.0005 0.0009 0.001 0.0014 0.0082 0.0004 0.0021 0.0002 0.0121 0.0064 0.0005 0.0001 0.0005 0.0003 0.0002 0.0003
None 0.0212 0.0108 0.0261 0.0036 0.0061 0.0114 0.0117 0.006 0.008 0.003 0.0039 0.0023 0.0352 0.0129 0.0068 0.0129 0.0042 0.007
Nuclear Periphery 0.0012 0.0004 0.0009 0.0005 0.0011 0.0007 0.021 0.0003 0.0013 0.0007 0.0007 0.0003 0.0013 0.0003 0.0011 0.0018 0.0004 0.0005
Nucleolus 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0005 0.0002 0.0001 0 0 0.0002 0.0001 0 0.0007 0.0001 0 0
Nucleus 0.0011 0.0007 0.0011 0.0009 0.001 0.0013 0.0118 0.0008 0.0012 0.0008 0.0005 0.0007 0.0012 0.0007 0.0017 0.0025 0.0008 0.0011
Peroxisomes 0.001 0.0004 0.0007 0.0122 0.001 0.0022 0.0169 0.0001 0.0106 0.0002 0.0078 0.0027 0.0056 0.0001 0.0031 0.0009 0.0001 0.0002
Punctate Nuclear 0.0014 0.0005 0.0009 0.0013 0.0008 0.0006 0.0089 0.0002 0.0024 0.0003 0.0002 0.0002 0.0006 0.0002 0.0014 0.0177 0.0002 0.0027
Vacuole 0.0024 0.0006 0.0036 0.0043 0.0064 0.0014 0.0045 0.0009 0.0029 0.0023 0.0038 0.0012 0.0019 0.0012 0.0019 0.0028 0.0013 0.0007
Vacuole Periphery 0.0005 0.0001 0.0006 0.0014 0.0011 0.0004 0.0037 0.0001 0.0006 0.0005 0.0019 0.0005 0.0003 0.0001 0.0003 0.0005 0.0004 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 47.3143 59.8529 54.4805 51.8113 69.4472 48.1803 50.2293 51.7371 60.8353 47.6989
Translational Efficiency 1.2246 1.1897 1.1687 1.1344 0.8993 1.0343 1.1263 1.2185 0.81 0.9741

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
ORF
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-4

YGR210C

YGR210C


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ygr210c-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available