Standard name
Human Ortholog
Description Ribosomal 40S subunit protein S0A; required for maturation of 18S rRNA along with Rps0Bp; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2; RPS0A has a paralog, RPS0B, that arose from the whole genome duplication;
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0.05 0 0 0 0 0 0.06 0.05 0.05 0.07 0 0 0 0.06 0.05 0 0 0 0 0 0 0
Cytoplasm 0.99 0.98 0.92 0.95 0.91 0.83 0.64 0.58 0.54 0.54 0.48 0.48 0.97 0.96 0.97 0.94 0.97 0.95 0.89 0.9 0.84 0.88 0.96 0.88
Endoplasmic Reticulum 0 0 0 0 0 0.08 0.13 0.1 0.18 0.19 0.14 0.19 0 0 0 0.05 0 0.05 0 0 0.09 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.09 0.12 0.31 0.28 0.29 0.34 0.45 0.39 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.09 0.05 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 1 5 10 12 10 5 7 16 0 0 0 2 0 1 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 1 1 1 0 0 0 0 0 0 0 0 0 0 0 1 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 12 5 7 7 12 14 15 12 11 16 7 4 6 17 18 10 0 0 2 0 0 0
Cytoplasm 82 100 226 169 191 185 198 197 131 125 100 106 155 136 168 286 327 285 78 93 220 37 109 84
Endoplasmic Reticulum 0 1 11 4 7 18 39 35 44 43 30 42 4 4 6 15 5 16 3 2 23 0 1 4
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Golgi 1 0 1 0 0 1 3 19 4 6 1 2 0 0 1 3 0 1 0 0 1 0 0 0
Mitochondria 1 1 5 4 18 26 97 93 70 78 94 85 0 0 0 3 3 1 1 1 5 0 0 0
Nucleus 0 0 0 0 0 1 1 2 0 1 0 1 2 1 0 0 0 2 0 2 0 1 0 0
Nuclear Periphery 0 0 1 0 0 1 1 0 0 2 2 3 0 0 0 0 0 0 0 0 1 0 0 0
Nucleolus 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 2 0 5 1 7 31 13 10 12 7 0 0 6 8 6 9 1 1 2 1 0 2
Unique Cell Count 83 102 245 177 210 223 310 338 244 230 209 219 159 142 174 305 337 301 88 104 261 43 114 97
Labelled Cell Count 84 102 259 183 229 245 368 404 288 283 258 278 168 145 187 335 359 326 88 104 261 43 114 97


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.3 12.3 14.7 15.6 15.5 14.2 14.2 14.5 13.3 13.6 13.3 13.7 17.1 16.5 15.6 19.3 19.1 20.1 – 18.0 17.2
Std Deviation (1e-4) 2.1 2.9 2.0 2.4 2.5 2.6 5.8 3.0 3.1 3.0 4.0 3.2 2.7 3.2 2.4 5.8 5.2 4.9 – 3.5 3.6
Intensity Change (Log2) – – – 0.09 0.07 -0.05 -0.05 -0.02 -0.14 -0.11 -0.14 -0.1 0.22 0.16 0.08 0.4 0.38 0.45 – 0.29 0.23


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 2.5 2.8 0 0 3.9 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -1.1 -0.8 -1.0 -0.6 -0.4 0.6 0.2 0.2 1.1 -0.2 -1.0 -0.7 0.4 0.2 -0.9
Cytoplasm 1.3 -0.5 -3.1 -7.8 -9.1 -9.6 -9.4 -10.5 -10.4 2.2 1.4 1.8 0.7 2.6 1.2
Endoplasmic Reticulum -1.2 -0.6 1.6 3.3 2.6 4.7 4.9 3.7 5.0 -1.0 -0.8 -0.5 0.2 -2.2 0.4
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 3.4 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 3.2 4.2 8.8 8.1 8.2 9.1 11.0 10.0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 4.3 2.9 2.5 3.0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 18.9832 28.6931 25.4217 22.1199 19.6055 24.8214 17.8753 30.5962 25.8965 24.8594 20.2174 26.7831 22.38 30.0386 23.5441 28.6072 25.8603 27.4601
Actin 0.0155 0.0012 0.0139 0.0014 0.0164 0.0036 0.0246 0.0012 0.0062 0.0046 0.0009 0.024 0.0186 0.0005 0.0494 0.0002 0.006 0.0011
Bud 0.0017 0.0006 0.0012 0.0021 0.0008 0.0015 0.001 0.001 0.0006 0.0011 0.0025 0.0005 0.0013 0.001 0.0015 0.0011 0.0005 0.001
Bud Neck 0.0009 0.0006 0.0009 0.0009 0.0008 0.0019 0.0011 0.0003 0.0008 0.0058 0.0007 0.0008 0.0011 0.0003 0.0013 0.0006 0.0006 0.0016
Bud Periphery 0.0022 0.0003 0.0013 0.0007 0.0004 0.0016 0.001 0.0007 0.0002 0.0005 0.0027 0.0002 0.0012 0.0011 0.0015 0.0001 0.0002 0.0008
Bud Site 0.003 0.0005 0.0006 0.0015 0.0005 0.0007 0.0027 0.0014 0.0007 0.0024 0.0005 0.0001 0.0041 0.0002 0.0127 0.0002 0.0007 0.0002
Cell Periphery 0.003 0.0007 0.0008 0.0004 0.0003 0.0008 0.0021 0.0023 0.0002 0.0004 0.0005 0.0001 0.0025 0.0002 0.0013 0.0002 0.0001 0.0003
Cytoplasm 0.6482 0.9168 0.8103 0.6693 0.623 0.7413 0.6353 0.9439 0.8171 0.6511 0.7127 0.8349 0.702 0.9529 0.7283 0.866 0.7992 0.8371
Cytoplasmic Foci 0.024 0.0062 0.0105 0.0185 0.0171 0.0154 0.0465 0.0048 0.0392 0.0236 0.0226 0.0226 0.0083 0.003 0.0279 0.0059 0.0365 0.0108
Eisosomes 0.0002 0 0.0001 0 0.0003 0.0001 0.0003 0 0 0.0001 0 0 0.0002 0 0.0003 0 0 0
Endoplasmic Reticulum 0.0723 0.0301 0.0336 0.0493 0.034 0.0837 0.0381 0.0121 0.0184 0.0049 0.0069 0.0152 0.0746 0.0146 0.0122 0.0056 0.0024 0.065
Endosome 0.0635 0.0159 0.0588 0.1284 0.0935 0.0679 0.0887 0.0073 0.04 0.0636 0.079 0.0593 0.0544 0.008 0.0377 0.0285 0.043 0.0452
Golgi 0.0109 0.0037 0.0092 0.0102 0.0077 0.0176 0.0179 0.0011 0.0167 0.0157 0.0117 0.0088 0.0189 0.0011 0.0081 0.0005 0.0452 0.0034
Lipid Particles 0.0106 0.0001 0.0017 0.0009 0.0106 0.0045 0.0524 0.0005 0.0078 0.0081 0.037 0.0039 0.0049 0.0001 0.0192 0.0001 0.0127 0.0001
Mitochondria 0.0146 0.0014 0.0051 0.0042 0.0156 0.0176 0.0081 0.0063 0.0021 0.0078 0.0568 0.0022 0.014 0.0017 0.0174 0.0002 0.0089 0.0059
None 0.0028 0.0002 0.0008 0.0008 0.0306 0.0009 0.0062 0.0004 0.0012 0.0004 0.0004 0.0005 0.0025 0.0004 0.0022 0.0003 0.0002 0.0005
Nuclear Periphery 0.0215 0.0016 0.0034 0.0068 0.0168 0.0087 0.0074 0.0013 0.0036 0.0065 0.0034 0.0044 0.0211 0.0035 0.0052 0.0104 0.0037 0.0051
Nucleolus 0.0013 0 0.0001 0.0003 0.0016 0.0003 0.0005 0.0001 0.0001 0.0003 0.0002 0 0.0002 0 0.0015 0 0.0001 0
Nucleus 0.0077 0.0009 0.0013 0.0031 0.0056 0.003 0.0029 0.001 0.0015 0.0015 0.0026 0.0014 0.0076 0.0013 0.0029 0.002 0.0013 0.0018
Peroxisomes 0.0052 0.0001 0.0009 0.0003 0.002 0.0061 0.0205 0.0002 0.0079 0.009 0.0122 0.0016 0.004 0 0.0126 0.0001 0.0264 0.0001
Punctate Nuclear 0.0036 0 0.0014 0.0003 0.0112 0.0007 0.0089 0 0.0006 0.0004 0.0019 0.0003 0.0016 0.0001 0.0102 0.0001 0.0003 0.0001
Vacuole 0.0657 0.0165 0.0344 0.0786 0.0778 0.0133 0.0281 0.0121 0.0281 0.1362 0.0337 0.0116 0.0467 0.0079 0.0344 0.0565 0.0079 0.0128
Vacuole Periphery 0.0216 0.0027 0.0096 0.022 0.0332 0.0088 0.0055 0.002 0.0071 0.056 0.011 0.0075 0.0105 0.0022 0.0123 0.0214 0.0041 0.0068

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 1464.664 1563.3867 1657.6996 1451.0502 1780.2544 1647.6521 1317.2823 1284.1727 1403.1514 1295.3666
Translational Efficiency 1.2429 1.2052 1.2355 1.4048 1.2345 1.1245 1.3728 1.3694 1.3121 1.4788

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Ribosomal 40S subunit protein S0A; required for maturation of 18S rRNA along with Rps0Bp; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2; RPS0A has a paralog, RPS0B, that arose from the whole genome duplication;
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Rps0a

Rps0a


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rps0a-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available