Standard name | |
---|---|
Human Ortholog | |
Description | Mitochondrial ribosomal protein of the small subunit |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 8 | 1 | 4 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 4 | 0 | 0 | 1 | 0 | 11 | 5 | 0 | 3 | 15 | 15 |
Bud | 12 | 2 | 3 | 14 | 7 | 2 | 5 | 13 | 10 | 5 | 13 | 14 | 0 | 2 | 10 | 2 | 4 | 5 | 3 | 2 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 |
Bud Site | 0 | 0 | 3 | 0 | 1 | 1 | 3 | 7 | 1 | 3 | 0 | 4 | 0 | 0 | 0 | 0 | 1 | 0 | – | – | – | – | – | – |
Cell Periphery | 18 | 13 | 4 | 3 | 3 | 1 | 1 | 2 | 3 | 4 | 2 | 4 | 9 | 11 | 10 | 5 | 5 | 7 | 1 | 0 | 0 | 0 | 2 | 1 |
Cytoplasm | 6 | 4 | 2 | 7 | 16 | 10 | 7 | 18 | 5 | 4 | 6 | 4 | 3 | 6 | 9 | 6 | 3 | 8 | 0 | 1 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 7 | 4 | 4 | 2 | 2 | 3 | 4 | 1 | 0 | 1 | 5 | 3 | 2 | 5 | 4 | 2 | 1 | 5 | 2 | 0 | 0 | 0 | 0 | 2 |
Endosome | 20 | 9 | 0 | 0 | 12 | 2 | 2 | 11 | 2 | 2 | 4 | 0 | 15 | 15 | 28 | 9 | 11 | 15 | 36 | 14 | 10 | 8 | 15 | 17 |
Golgi | 16 | 30 | 0 | 0 | 3 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 29 | 40 | 70 | 6 | 2 | 9 | 35 | 26 | 7 | 18 | 44 | 53 |
Mitochondria | 517 | 253 | 169 | 239 | 264 | 265 | 386 | 482 | 208 | 327 | 294 | 433 | 320 | 372 | 321 | 8 | 3 | 11 | 490 | 234 | 128 | 144 | 263 | 245 |
Nucleus | 1 | 1 | 1 | 0 | 0 | 3 | 3 | 2 | 4 | 1 | 0 | 2 | 0 | 1 | 1 | 4 | 2 | 2 | 1 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 11 | 8 | 7 | 14 | 18 | 46 | 62 | 36 | 30 | 39 | 40 | 44 | 10 | 11 | 5 | 2 | 0 | 2 | 4 | 3 | 1 | 3 | 4 | 2 |
Nucleolus | 19 | 6 | 2 | 12 | 18 | 23 | 37 | 40 | 25 | 41 | 22 | 28 | 16 | 7 | 8 | 0 | 1 | 1 | 2 | 0 | 0 | 0 | 2 | 4 |
Peroxisomes | 14 | 2 | 1 | 7 | 9 | 2 | 1 | 12 | 1 | 5 | 2 | 3 | 27 | 15 | 14 | 8 | 9 | 10 | 27 | 8 | 12 | 19 | 35 | 30 |
SpindlePole | 3 | 3 | 3 | 2 | 2 | 3 | 6 | 14 | 1 | 5 | 2 | 4 | 4 | 4 | 5 | 6 | 5 | 5 | 4 | 1 | 0 | 3 | 0 | 0 |
Vac/Vac Membrane | 110 | 63 | 14 | 9 | 13 | 10 | 9 | 24 | 10 | 7 | 6 | 3 | 37 | 25 | 39 | 16 | 13 | 14 | 31 | 14 | 5 | 5 | 11 | 9 |
Unique Cell Count | 603 | 297 | 177 | 252 | 300 | 290 | 415 | 533 | 222 | 347 | 311 | 452 | 355 | 407 | 409 | 56 | 47 | 71 | 656 | 317 | 171 | 209 | 401 | 388 |
Labelled Cell Count | 762 | 399 | 217 | 309 | 370 | 371 | 526 | 662 | 302 | 445 | 397 | 546 | 474 | 518 | 524 | 74 | 61 | 94 | 656 | 317 | 171 | 209 | 401 | 388 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.5 | 8.2 | 5.2 | 6.3 | 7.2 | 6.1 | 5.7 | 6.3 | 5.6 | 6.1 | 5.7 | 5.8 | 8.2 | 8.0 | 7.7 | 8.8 | 8.1 | 8.2 | 8.1 | 9.1 | 9.0 |
Std Deviation (1e-4) | 1.4 | 1.5 | 1.4 | 1.8 | 1.9 | 1.6 | 1.7 | 1.7 | 1.4 | 1.6 | 1.7 | 1.5 | 1.4 | 1.4 | 1.3 | 2.2 | 2.1 | 2.1 | 1.6 | 1.7 | 1.7 |
Intensity Change (Log2) | – | – | – | 0.29 | 0.48 | 0.24 | 0.14 | 0.28 | 0.12 | 0.23 | 0.15 | 0.17 | 0.67 | 0.63 | 0.58 | 0.76 | 0.65 | 0.67 | 0.65 | 0.81 | 0.8 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 2.0 | 0 | 0 | 0 | 0 | 1.6 | 0 | 1.5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 2.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.6 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.9 | 4.3 | 5.9 | 0 | 0 | 0 |
Mitochondria | -0.3 | -2.7 | -1.7 | -1.1 | -2.1 | -0.8 | -0.6 | -0.5 | 0.2 | -2.1 | -1.7 | -5.1 | -12.4 | -12.9 | -12.8 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0.8 | 1.0 | 3.9 | 3.8 | 1.4 | 3.3 | 2.8 | 3.2 | 2.4 | -0.7 | -0.8 | 0 | 0 | 0 | 0 |
Nucleolus | 2.1 | 2.6 | 3.2 | 3.5 | 3.1 | 4.0 | 4.2 | 2.9 | 2.7 | 2.0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.4 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -2.0 | -1.6 | -2.1 | -3.3 | -1.7 | -1.4 | -3.3 | -3.2 | 0 | 0.9 | -0.8 | 0.6 | 4.0 | 3.7 | 2.7 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 4.5985 | 4.3828 | 4.1726 | 4.0148 | 4.0427 | 4.2116 | 2.8772 | 3.1256 | 3.1013 | 2.772 | 3.0719 | 2.8521 | 4.6099 | 4.8996 | 4.4686 | 4.1514 | 4.0319 | 4.465 |
Actin | 0.0158 | 0.0055 | 0.0073 | 0.0011 | 0.0008 | 0.0036 | 0.056 | 0.0104 | 0.018 | 0.0103 | 0.0055 | 0.0133 | 0.0423 | 0.025 | 0.0181 | 0.0437 | 0.01 | 0.002 |
Bud | 0.0002 | 0.0024 | 0.0015 | 0.0001 | 0.0001 | 0.0002 | 0.0007 | 0.0038 | 0.0008 | 0.0009 | 0.0003 | 0.0008 | 0.0015 | 0.0011 | 0.0006 | 0.0002 | 0.0003 | 0.0001 |
Bud Neck | 0.0005 | 0.0002 | 0.0004 | 0 | 0 | 0.006 | 0.0018 | 0.0003 | 0.001 | 0.0025 | 0 | 0.0006 | 0.0012 | 0.0003 | 0.0004 | 0.0001 | 0.0001 | 0.0001 |
Bud Periphery | 0.0005 | 0.0042 | 0.004 | 0.0002 | 0.0001 | 0.0008 | 0.0013 | 0.0082 | 0.0017 | 0.0013 | 0.0008 | 0.0007 | 0.0037 | 0.0019 | 0.0012 | 0.0005 | 0.0009 | 0.0002 |
Bud Site | 0.0005 | 0.0041 | 0.002 | 0.0001 | 0.0002 | 0.001 | 0.0082 | 0.0022 | 0.0026 | 0.0036 | 0.0002 | 0.0014 | 0.0046 | 0.0028 | 0.0029 | 0.0004 | 0.0002 | 0.0001 |
Cell Periphery | 0.0002 | 0.0003 | 0.0003 | 0.0001 | 0 | 0.0005 | 0.0016 | 0.0003 | 0.0004 | 0.0002 | 0.0001 | 0.0001 | 0.0007 | 0.0005 | 0.0006 | 0.0004 | 0.0001 | 0.0001 |
Cytoplasm | 0.005 | 0.0005 | 0.0014 | 0 | 0.0001 | 0.0011 | 0.0027 | 0.0008 | 0.001 | 0.0013 | 0 | 0.0037 | 0.0037 | 0.0005 | 0.0008 | 0.0001 | 0.0001 | 0.0007 |
Cytoplasmic Foci | 0.0117 | 0.0103 | 0.0111 | 0.0032 | 0.0076 | 0.0077 | 0.0189 | 0.0148 | 0.004 | 0.0432 | 0.0008 | 0.0133 | 0.0165 | 0.0113 | 0.0179 | 0.0061 | 0.005 | 0.009 |
Eisosomes | 0.0004 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.0002 | 0.0009 | 0.0002 | 0.0011 | 0.0002 | 0.0003 | 0.0002 | 0.001 | 0.0008 | 0.0006 | 0.0011 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0018 | 0.0003 | 0.0014 | 0 | 0.0001 | 0.0004 | 0.0014 | 0.0004 | 0.0007 | 0.0004 | 0.0001 | 0.0003 | 0.0017 | 0.0001 | 0.0005 | 0.0001 | 0.0001 | 0.0003 |
Endosome | 0.0366 | 0.0301 | 0.0258 | 0.0036 | 0.007 | 0.0106 | 0.0339 | 0.0403 | 0.0202 | 0.023 | 0.003 | 0.0382 | 0.0327 | 0.0167 | 0.0281 | 0.0036 | 0.0048 | 0.0265 |
Golgi | 0.0252 | 0.0406 | 0.0351 | 0.0103 | 0.0269 | 0.0248 | 0.0429 | 0.0956 | 0.0311 | 0.0442 | 0.0063 | 0.0499 | 0.0738 | 0.0337 | 0.0209 | 0.0126 | 0.0055 | 0.04 |
Lipid Particles | 0.0438 | 0.0096 | 0.0166 | 0.0068 | 0.0089 | 0.0106 | 0.0558 | 0.0119 | 0.0038 | 0.0023 | 0.0022 | 0.0025 | 0.0434 | 0.0163 | 0.0106 | 0.0025 | 0.0012 | 0.0218 |
Mitochondria | 0.8086 | 0.8316 | 0.8517 | 0.9381 | 0.8948 | 0.9101 | 0.7178 | 0.7766 | 0.8913 | 0.8463 | 0.9601 | 0.83 | 0.6899 | 0.835 | 0.8722 | 0.8846 | 0.8614 | 0.8808 |
None | 0.0007 | 0.0003 | 0.0007 | 0 | 0.0001 | 0.0025 | 0.0013 | 0.0121 | 0.0127 | 0.0001 | 0.0001 | 0.0001 | 0.0149 | 0.0003 | 0.0003 | 0.0003 | 0.0001 | 0.0003 |
Nuclear Periphery | 0.0048 | 0.0003 | 0.0062 | 0 | 0.0013 | 0.0008 | 0.0102 | 0.0002 | 0.0004 | 0.0001 | 0 | 0.0001 | 0.0051 | 0 | 0.0002 | 0 | 0.0001 | 0.0018 |
Nucleolus | 0.0004 | 0.0003 | 0.0003 | 0 | 0.0003 | 0.0004 | 0.0027 | 0.0003 | 0.0001 | 0.0001 | 0 | 0 | 0.003 | 0.0002 | 0.0013 | 0 | 0 | 0.0001 |
Nucleus | 0.001 | 0.0003 | 0.002 | 0 | 0.0002 | 0.0003 | 0.0055 | 0.0002 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0024 | 0.0001 | 0.0004 | 0 | 0.0001 | 0.0002 |
Peroxisomes | 0.0275 | 0.0503 | 0.0227 | 0.0344 | 0.0352 | 0.0099 | 0.0243 | 0.0142 | 0.0046 | 0.0169 | 0.0187 | 0.0412 | 0.0331 | 0.0502 | 0.0125 | 0.043 | 0.1075 | 0.0106 |
Punctate Nuclear | 0.0015 | 0.0002 | 0.0004 | 0 | 0.0152 | 0.0014 | 0.0034 | 0.0001 | 0.0005 | 0.0002 | 0 | 0.0001 | 0.0135 | 0.0001 | 0.0004 | 0 | 0 | 0.0016 |
Vacuole | 0.0042 | 0.0028 | 0.003 | 0.0005 | 0.0003 | 0.0024 | 0.0049 | 0.003 | 0.0011 | 0.0013 | 0.0004 | 0.0016 | 0.0063 | 0.0015 | 0.007 | 0.0001 | 0.0008 | 0.001 |
Vacuole Periphery | 0.0089 | 0.0058 | 0.006 | 0.0011 | 0.0008 | 0.0046 | 0.004 | 0.0041 | 0.0026 | 0.0013 | 0.0011 | 0.0019 | 0.0049 | 0.0018 | 0.0022 | 0.0003 | 0.0018 | 0.0025 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 26.5082 | 29.6209 | 15.386 | 36.3183 | 32.221 | 56.2988 | 41.1808 | 42.4494 | 52.2302 | 43.8647 |
Translational Efficiency | 2.7414 | 2.3034 | 4.0285 | 2.2823 | 2.3582 | 1.5934 | 2.2281 | 2.4905 | 1.8989 | 2.0257 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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