Standard name
Human Ortholog
Description Protein involved in the regulation of cell wall synthesis; proposed to be involved in coordinating cell cycle progression with cell wall integrity
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.25 0.3 0.24 0.24 0.18 0.25 0.11 0.13 0.33 0.16 0.25 0.18 0.22 0.17 0.09 0.17 0.16 0.19 0.2 0.19 0.18 0.2 0.24 0.18
Bud 0.06 0 0.16 0.1 0 0.09 0.08 0.09 0.16 0.13 0.12 0.11 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0.05 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.24 0.27 0.29 0.26 0 0.05 0.06 0.05 0.12 0.08 0.18 0.08 0.06 0.09 0.11 0.28 0.28 0.25 0.13 0.15 0.13 0 0 0
Endoplasmic Reticulum 0.2 0.26 0.11 0.08 0 0.09 0 0.06 0.06 0 0.08 0 0.09 0.06 0 0 0.06 0 0.14 0.1 0.13 0 0 0.05
Endosome 0 0 0.05 0 0 0 0.08 0.07 0 0 0.07 0.05 0 0 0 0.05 0 0 0.07 0.06 0.14 0.06 0.08 0.06
Golgi 0.36 0.4 0.23 0.25 0.44 0.22 0.16 0.13 0.19 0.11 0.12 0.13 0.7 0.64 0.56 0.51 0.65 0.62 0.39 0.35 0.3 0.6 0.57 0.57
Mitochondria 0.27 0.16 0.42 0.48 0.69 0.79 0.7 0.69 0.58 0.74 0.56 0.74 0.48 0.45 0.45 0.08 0.1 0.08 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0.06 0.09 0.05 0 0.07 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.05 0.06 0 0 0 0 0 0 0 0.06 0 0 0 0.05 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 64 88 30 30 33 39 26 38 73 42 41 45 90 79 44 71 37 37 59 67 27 63 80 70
Bud 16 8 20 12 6 15 18 27 36 34 20 27 3 10 6 8 5 5 5 12 1 5 2 9
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4 3 0 0 0 2
Bud Site 0 0 0 0 0 2 1 3 4 2 0 5 0 0 0 0 0 0 – – – – – –
Cell Periphery 5 6 0 1 0 2 0 4 10 7 10 7 5 8 3 8 5 3 1 0 0 0 0 0
Cytoplasm 61 79 36 33 8 8 14 15 27 21 30 20 26 44 52 117 67 49 38 54 19 4 4 8
Endoplasmic Reticulum 51 77 14 10 6 14 8 16 14 11 13 7 37 27 20 15 15 8 42 34 20 13 14 21
Endosome 3 0 6 3 8 6 19 20 8 8 11 12 5 7 14 21 5 2 22 20 22 19 26 23
Golgi 90 117 28 31 82 34 38 37 41 28 19 32 282 300 275 209 154 122 118 124 46 191 193 225
Mitochondria 69 46 52 61 129 125 164 199 126 196 92 182 193 212 222 31 23 16 4 8 5 5 2 12
Nucleus 0 1 0 0 1 0 1 0 0 0 0 1 0 0 0 2 0 0 0 0 0 0 0 1
Nuclear Periphery 0 0 0 0 1 3 1 2 0 2 3 0 1 1 1 1 1 0 0 0 0 0 0 1
Nucleolus 4 0 4 4 4 9 22 15 3 19 1 12 5 7 16 6 6 4 0 0 0 0 0 0
Peroxisomes 0 0 0 0 5 2 2 5 3 2 0 2 2 2 5 1 0 0 1 1 1 2 5 5
SpindlePole 0 0 0 1 0 1 2 6 3 3 1 4 1 1 0 9 4 0 1 3 0 3 2 0
Vac/Vac Membrane 0 4 2 2 6 2 11 16 4 1 4 5 7 10 14 24 6 6 1 16 5 5 2 8
Unique Cell Count 251 291 124 126 188 158 234 290 219 265 165 247 404 470 488 413 238 197 304 355 155 317 341 395
Labelled Cell Count 363 426 192 188 289 262 327 403 352 376 245 361 657 708 672 523 328 252 304 355 155 317 341 395


Late Golgi, Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 19.1 20.7 13.1 10.9 12.9 11.7 10.0 10.1 11.0 10.1 9.9 10.3 20.5 19.8 19.3 18.4 22.9 23.4 18.7 18.0 19.0
Std Deviation (1e-4) 3.5 4.2 2.3 3.1 2.5 2.5 2.4 2.2 2.1 2.0 2.0 1.9 4.0 4.5 5.3 5.0 6.5 6.4 3.9 3.8 3.9
Intensity Change (Log2) – – – -0.27 -0.03 -0.17 -0.39 -0.38 -0.26 -0.38 -0.41 -0.36 0.64 0.59 0.55 0.48 0.8 0.83 0.51 0.45 0.54


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -0.1 -1.4 0.1 -3.2 -2.8 1.8 -2.0 0.1 -1.4 -0.4 -1.9 -4.6 -1.7 -2.0 -1.2
Bud -1.6 -4.0 -1.7 -2.5 -2.0 0.1 -0.9 -1.0 -1.4 -7.3 -6.3 -7.3 -6.2 -5.0 -4.4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.5 -6.2 -5.5 -6.0 -6.8 -3.8 -5.5 -2.2 -5.3 -6.8 -5.7 -5.2 -0.2 -0.2 -0.8
Endoplasmic Reticulum -0.9 -2.9 -0.7 -3.0 -2.1 -1.6 -2.7 -1.0 -3.3 -0.7 -2.2 -3.1 -3.3 -1.7 -2.5
Endosome 0 -0.2 -0.4 1.2 0.8 -0.5 0 0.7 0 0 0 0 0.1 0 0
Golgi 0.4 3.8 -0.2 -1.5 -2.5 -0.9 -3.1 -2.5 -2.4 9.3 8.2 6.7 5.5 7.6 6.9
Mitochondria 1.0 4.7 6.4 5.2 5.1 2.8 6.1 2.3 6.0 1.1 0.6 0.7 -9.3 -7.2 -7.2
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 1.0 2.1 0.9 0 1.5 0 0.7 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 1.8 0 0 0 0 0 0 0 1.9 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 18.7112 20.953 21.3864 21.4725 20.5385 22.0043 12.5473 17.8306 16.7065 16.0336 16.6629 15.4505 16.843 18.092 19.7 18.0106 17.3675 18.1645
Actin 0.1264 0.0442 0.0585 0.0329 0.0391 0.042 0.1751 0.1725 0.1409 0.0813 0.0996 0.0989 0.2382 0.1358 0.0772 0.0172 0.0134 0.115
Bud 0.0012 0.0033 0.0084 0.0048 0.0014 0.0002 0.0045 0.0015 0.0108 0.0021 0.0004 0.0003 0.0072 0.0042 0.02 0.0018 0.0124 0.0007
Bud Neck 0.0045 0.0013 0.0023 0.0022 0.0026 0.0027 0.004 0.0006 0.0016 0.0012 0.0007 0.0011 0.002 0.0009 0.0012 0.0009 0.0011 0.0011
Bud Periphery 0.0015 0.0019 0.0053 0.0052 0.0024 0.0002 0.005 0.0006 0.0094 0.0007 0.0003 0.0003 0.0023 0.0015 0.0122 0.0009 0.0156 0.0007
Bud Site 0.0027 0.0169 0.01 0.0023 0.0016 0.0002 0.0084 0.0018 0.0041 0.0016 0.0004 0.0002 0.0444 0.0054 0.0036 0.0004 0.061 0.0006
Cell Periphery 0.001 0.001 0.0008 0.0011 0.0006 0.0002 0.0005 0.0007 0.0008 0.0002 0.0001 0.0001 0.0004 0.0002 0.0006 0.0007 0.0018 0.0001
Cytoplasm 0.0303 0.0389 0.0891 0.1113 0.0319 0.0036 0.0172 0.0208 0.0614 0.0743 0.0385 0.0038 0.0282 0.0563 0.0814 0.2953 0.1007 0.0047
Cytoplasmic Foci 0.0726 0.1304 0.0803 0.1002 0.1027 0.0374 0.0683 0.0546 0.0869 0.1108 0.081 0.0326 0.0706 0.1338 0.0993 0.1092 0.0649 0.0345
Eisosomes 0.0006 0.0003 0.0001 0.0001 0.0002 0.0001 0.001 0.0001 0.0004 0 0 0.0002 0.0003 0.0001 0.0003 0 0.0001 0.0002
Endoplasmic Reticulum 0.008 0.0015 0.003 0.0045 0.0081 0.0011 0.0049 0.0004 0.0074 0.002 0.0059 0.0021 0.0021 0.0013 0.0021 0.006 0.002 0.0007
Endosome 0.1905 0.2421 0.2792 0.3305 0.3153 0.1352 0.1597 0.158 0.1991 0.3403 0.221 0.0931 0.1244 0.2368 0.1921 0.2088 0.2023 0.0874
Golgi 0.4406 0.4309 0.3898 0.3292 0.4341 0.7505 0.4122 0.5523 0.4338 0.3492 0.5329 0.7274 0.453 0.402 0.4804 0.2673 0.5102 0.7069
Lipid Particles 0.0404 0.0279 0.0238 0.0243 0.0222 0.0085 0.0413 0.0017 0.0165 0.016 0.0052 0.0134 0.0058 0.007 0.0053 0.0714 0.0007 0.0043
Mitochondria 0.029 0.0148 0.0162 0.0068 0.0195 0.0121 0.0253 0.019 0.006 0.0035 0.0026 0.0086 0.0036 0.0061 0.0103 0.0015 0.0051 0.0385
None 0.0097 0.0003 0.0009 0.0003 0.0003 0 0.0288 0.0001 0.001 0.0001 0.0001 0.0009 0.0005 0.0001 0.0002 0.0002 0.0007 0.0001
Nuclear Periphery 0.007 0.001 0.0018 0.001 0.0026 0.0001 0.0026 0.0001 0.0016 0.0005 0.0008 0.0059 0.0003 0.0003 0.0006 0.0023 0.0006 0.0001
Nucleolus 0.0012 0.0007 0.001 0.0002 0.0001 0 0.0035 0.0001 0.0001 0 0 0.0002 0.0001 0 0.0001 0.0001 0.0001 0.0001
Nucleus 0.0024 0.0009 0.0012 0.0005 0.0005 0 0.0033 0.0002 0.0004 0.0001 0.0001 0.0005 0.0003 0.0001 0.0003 0.0012 0.0009 0.0001
Peroxisomes 0.0092 0.0047 0.0043 0.0019 0.0034 0.0013 0.0095 0.0037 0.0062 0.008 0.001 0.005 0.0139 0.0034 0.0029 0.0009 0.0015 0.0021
Punctate Nuclear 0.0045 0.0025 0.0016 0.0006 0.0006 0.0001 0.0158 0.0001 0.0008 0.0002 0.0001 0.0034 0.0006 0.0002 0.0003 0.0003 0.0003 0.0002
Vacuole 0.0119 0.024 0.0158 0.0324 0.0063 0.0017 0.006 0.0087 0.0062 0.0047 0.0065 0.0006 0.001 0.0025 0.0062 0.0103 0.003 0.0007
Vacuole Periphery 0.0048 0.0106 0.0067 0.0076 0.0044 0.0026 0.0031 0.0025 0.0046 0.0031 0.0027 0.0013 0.0008 0.0016 0.0034 0.0033 0.0016 0.0011

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 87.2256 59.1306 64.6107 70.0383 73.5097 74.8272 62.9076 76.2699 63.5376 71.9949
Translational Efficiency 1.0698 1.2551 1.0707 1.0382 0.9657 0.9824 0.8974 0.7355 0.9924 0.9365

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein involved in the regulation of cell wall synthesis; proposed to be involved in coordinating cell cycle progression with cell wall integrity
Localization
Cell Percentages cytoplasm (17%), Golgi (6%), mixed (42%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Smi1

Smi1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Smi1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available