Standard name
Human Ortholog
Description Nitric oxide oxidoreductase; flavohemoglobin that plays role in oxidative and nitrosative stress responses; protects against nitration of cellular targets and against cell growth inhibition under aerobic or anaerobic conditions; yeast flavohemoglobin Yhb1p and human homolog neuroglobin NGB protect cells against alpha-synuclein cytotoxicity and aggregate formation; protein increases in abundance, relocalizes from nucleus to cytoplasmic foci upon DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0.05 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.07 0 0.09 0.14 0.13
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.96 1.0 0.99 0.95 0.91 0.83 0.68 0.56 0.65 0.57 0.58 0.6 0.97 0.96 0.9 0.93 0.96 0.98 0.88 0.88 0.9 0.83 0.8 0.8
Endoplasmic Reticulum 0 0 0 0 0 0.05 0.15 0.17 0.15 0.2 0.14 0.2 0 0 0 0 0.05 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0.06 0 0.05 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.06 0.07 0.11 0.16 0.12 0.09 0.1 0.08 0 0 0.06 0.09 0.05 0.05 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 7 15 13 13 26 26 18 0 2 6 0 0 0 0 0 0 0 0 3
Bud 0 1 0 0 3 1 2 5 2 8 6 5 0 4 3 0 0 0 11 14 10 14 61 62
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 3
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Cell Periphery 0 1 3 2 7 6 11 16 17 20 13 10 1 6 7 2 5 1 0 0 0 0 0 0
Cytoplasm 152 201 277 260 466 363 418 319 283 294 225 252 347 492 596 86 110 40 143 187 243 134 364 382
Endoplasmic Reticulum 0 0 1 2 10 20 94 99 64 104 53 84 2 2 13 2 6 2 1 0 3 2 1 1
Endosome 0 0 0 0 0 4 1 0 1 0 0 0 0 0 3 0 0 1 0 1 1 1 0 0
Golgi 0 0 0 2 1 9 19 37 18 27 31 17 2 0 8 0 0 0 0 0 0 0 0 0
Mitochondria 5 0 2 2 4 12 20 9 8 24 18 11 0 0 0 0 0 0 0 0 1 0 1 2
Nucleus 0 0 0 1 1 0 1 3 2 3 2 1 2 2 0 2 0 0 1 3 0 3 8 5
Nuclear Periphery 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0
Nucleolus 2 0 0 1 1 3 4 11 5 13 8 12 0 0 0 0 0 0 0 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 3 5 0 0 0 1 0 2 1 2 2
Vac/Vac Membrane 2 0 5 9 30 32 65 90 50 49 37 34 6 19 40 8 6 2 0 3 2 2 9 4
Unique Cell Count 159 201 281 275 514 435 612 571 433 519 386 422 357 512 660 92 115 41 163 214 270 162 456 476
Labelled Cell Count 161 203 288 279 523 457 650 603 463 568 419 444 360 531 682 100 127 46 163 214 270 162 456 476


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.4 23.7 14.6 20.5 25.8 26.0 30.4 34.6 37.0 39.0 39.8 44.0 25.8 29.8 30.1 20.5 27.3 36.0 21.8 23.5 23.7
Std Deviation (1e-4) 6.0 13.2 7.2 9.3 12.6 11.8 12.0 12.5 13.0 13.3 15.3 14.1 15.7 17.8 18.6 11.4 17.4 20.9 13.7 13.3 12.9
Intensity Change (Log2) 0.5 0.83 0.84 1.06 1.25 1.35 1.42 1.45 1.6 0.83 1.03 1.05 0.49 0.91 1.31 0.58 0.69 0.7


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 2.9 3.8 4.4 3.5 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 1.6 2.3 2.3 1.9 1.3 0 0 0 0 0 0
Cytoplasm -2.6 -4.3 -6.4 -10.1 -12.8 -10.5 -12.5 -11.9 -11.7 -1.2 -2.0 -4.5 -2.6 -1.8 -0.5
Endoplasmic Reticulum 0 0 3.3 6.8 7.2 6.5 7.9 6.3 7.8 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 3.0 4.4 3.5 3.9 4.9 3.4 0 0 0 0 0 0
Mitochondria 0 0 1.9 2.3 0 0 3.0 3.0 1.8 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 1.1 2.7 3.3 4.6 6.1 4.8 4.1 4.1 3.6 0 1.5 2.8 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 17.8962 18.1708 17.4433 22.2867 14.4274 16.2284 15.3202 15.1102 16.0286 13.3046 16.199 16.5469 15.3901 19.3224 17.5442 17.3498 17.9653 20.2841
Actin 0.0451 0.0034 0.0487 0.0562 0.0001 0.0294 0.0175 0.0063 0.0102 0.0263 0.0007 0.0007 0.0197 0.0052 0.0081 0.0068 0.0066 0.0099
Bud 0.0013 0.0086 0.0047 0.0107 0 0.0006 0.0012 0.0048 0.001 0.0016 0.0007 0.0017 0.001 0.0086 0.0057 0.0026 0.0044 0.0049
Bud Neck 0.0005 0.0005 0.001 0.0041 0.0008 0.0021 0.0007 0.0004 0.0004 0.0008 0.0004 0.0011 0.0012 0.0003 0.0003 0.0008 0.0012 0.0013
Bud Periphery 0.002 0.0025 0.0027 0.0079 0 0.0003 0.0013 0.0028 0.0005 0.0025 0.0001 0.0006 0.0012 0.0034 0.002 0.003 0.0047 0.0041
Bud Site 0.0024 0.0073 0.0134 0.044 0 0.0002 0.0013 0.0072 0.0007 0.0071 0.0002 0 0.0013 0.0048 0.0021 0.0014 0.005 0.0007
Cell Periphery 0.0007 0.0005 0.0004 0.0005 0 0.0001 0.0003 0.0005 0.0001 0.0002 0 0 0.0005 0.0002 0.0001 0.0001 0.0001 0.0002
Cytoplasm 0.5261 0.934 0.8878 0.6518 0.993 0.831 0.7798 0.9304 0.9397 0.8293 0.9768 0.9685 0.8092 0.9439 0.919 0.8809 0.9312 0.926
Cytoplasmic Foci 0.0256 0.0068 0.0043 0.037 0.0009 0.0047 0.0224 0.0058 0.0051 0.0163 0.0038 0.0032 0.013 0.0055 0.0053 0.0106 0.0154 0.0064
Eisosomes 0.0005 0 0.0006 0.0002 0 0.0002 0.0002 0 0.0001 0.0004 0 0 0.0002 0 0.0001 0.0001 0 0.0002
Endoplasmic Reticulum 0.0656 0.0012 0.0031 0.0025 0.0013 0.0046 0.0158 0.0017 0.0036 0.0009 0.0024 0.0044 0.0114 0.0013 0.0029 0.004 0.0007 0.0022
Endosome 0.0359 0.0011 0.0032 0.0319 0.0003 0.052 0.0269 0.0048 0.0045 0.0083 0.0019 0.0013 0.0216 0.0011 0.011 0.02 0.0064 0.0016
Golgi 0.014 0.0002 0.0042 0.0857 0 0.0313 0.0076 0.0018 0.006 0.0072 0.0001 0.0001 0.0034 0.0002 0.0027 0.0084 0.0029 0.0013
Lipid Particles 0.0156 0.0005 0.0005 0.0053 0 0.0058 0.0277 0.0003 0.0012 0.037 0 0.0003 0.0043 0.0002 0.0021 0.0047 0.0021 0.0005
Mitochondria 0.0289 0.001 0.0081 0.0074 0 0.0089 0.0301 0.0041 0.0095 0.0221 0.0001 0.0002 0.0225 0.0004 0.0032 0.0044 0.0016 0.003
None 0.0112 0.0098 0.0062 0.0229 0.0005 0.0013 0.0194 0.0124 0.0044 0.0017 0.0028 0.001 0.0068 0.0062 0.0171 0.0103 0.001 0.0034
Nuclear Periphery 0.1642 0.002 0.002 0.002 0.0004 0.0024 0.0242 0.0024 0.0037 0.0022 0.0022 0.003 0.0235 0.0037 0.0036 0.0114 0.0013 0.0048
Nucleolus 0.0011 0.0022 0.0001 0.0007 0 0.0001 0.0004 0.0002 0.0001 0.0052 0 0 0.0011 0.0003 0.0002 0.001 0.0002 0.0004
Nucleus 0.0167 0.0096 0.0062 0.0068 0.0024 0.0206 0.0093 0.007 0.0055 0.0037 0.0052 0.0111 0.0367 0.0093 0.0089 0.0134 0.0068 0.0233
Peroxisomes 0.0145 0.0002 0.0005 0.0072 0 0.0007 0.0027 0.0011 0.0015 0.0169 0.0001 0 0.0008 0.0003 0.0015 0.0024 0.0059 0.0002
Punctate Nuclear 0.0097 0.0014 0.0009 0.0133 0.0001 0.0014 0.0024 0.0012 0.001 0.0082 0.001 0.0018 0.0076 0.0033 0.0016 0.0102 0.001 0.0038
Vacuole 0.0057 0.0057 0.0011 0.0014 0.0001 0.001 0.0052 0.004 0.0008 0.0011 0.0011 0.0006 0.0092 0.0017 0.0018 0.0025 0.0012 0.0011
Vacuole Periphery 0.0128 0.0014 0.0003 0.0005 0 0.0014 0.0035 0.0006 0.0006 0.0009 0.0003 0.0002 0.0039 0.0003 0.0008 0.0012 0.0003 0.0006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 148.0401 170.1499 220.19 337.9546 433.3562 108.4414 123.8344 131.8206 152.4052 336.7227
Translational Efficiency 1.5832 1.8212 2.1657 1.4222 1.9804 1.4463 1.7311 1.7925 1.9887 1.8303

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Nitric oxide oxidoreductase; flavohemoglobin that plays role in oxidative and nitrosative stress responses; protects against nitration of cellular targets and against cell growth inhibition under aerobic or anaerobic conditions; yeast flavohemoglobin Yhb1p and human homolog neuroglobin NGB protect cells against alpha-synuclein cytotoxicity and aggregate formation; protein increases in abundance, relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Yhb1

Yhb1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yhb1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available