Standard name
Human Ortholog
Description Alpha subunit of heterooctameric phosphofructokinase; involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0.08 0.07 0.07 0.09 0.09 0.14 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.99 0.98 0.99 0.99 0.95 0.91 0.9 0.89 0.86 0.82 0.82 0.99 0.99 1.0 0.98 0.96 1.0 0.97 0.94 0.94 0.92 0.92 0.93
Endoplasmic Reticulum 0 0 0 0 0 0 0.05 0.06 0.05 0.09 0.12 0.08 0 0 0 0.05 0.06 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 0 1 1 0 3 6 5 8 0 0 0 0 0 0 0 0 0 0 0 0
Bud 1 1 2 1 0 1 0 8 2 2 1 3 1 0 0 0 0 0 0 0 1 2 2 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 2 5 2 7 19 37 44 22 40 28 55 0 3 0 2 1 1 0 0 0 0 0 0
Cytoplasm 178 320 378 330 289 412 444 526 302 375 263 327 166 203 201 150 155 149 167 297 374 109 190 134
Endoplasmic Reticulum 0 2 7 4 7 12 25 35 18 39 37 32 0 2 1 8 10 9 0 0 11 1 6 3
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 1 0 1 1 0 0 0 1 0 0 0 0 0 0 0 1
Nucleus 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 2 1 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 1 1 3 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 1 1 3 0 0 0 2 0 1 1 0 3 1 0 6 3 0 1 0
Unique Cell Count 179 323 385 332 293 434 488 587 338 438 319 400 167 205 202 153 161 149 173 316 398 119 206 145
Labelled Cell Count 181 325 392 338 303 446 509 617 351 462 337 429 167 210 203 161 170 160 173 316 398 119 206 145


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 206.5 173.0 108.1 104.9 96.1 97.8 92.4 92.7 83.9 82.5 79.3 80.9 178.0 176.1 153.7 195.9 214.6 216.7 174.8 195.6 192.7
Std Deviation (1e-4) 41.3 38.4 23.0 25.6 28.3 25.0 25.6 24.9 23.9 23.5 20.8 24.5 46.1 44.2 39.6 52.8 60.6 70.8 54.1 54.2 60.6
Intensity Change (Log2) – – – -0.04 -0.17 -0.14 -0.23 -0.22 -0.37 -0.39 -0.45 -0.42 0.72 0.7 0.51 0.86 0.99 1.0 0.69 0.86 0.83


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 1.1 2.6 4.3 4.3 3.7 4.9 4.7 6.6 0 0 0 0 0 0
Cytoplasm 1.5 0.5 -2.5 -4.5 -5.1 -5.0 -6.4 -7.3 -7.6 1.1 0.8 1.3 -0.1 -1.3 1.7
Endoplasmic Reticulum -0.7 0.5 0.9 2.6 3.1 2.6 4.4 5.3 4.0 0 0 0 0 2.7 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 156.8588 220.0413 193.0001 187.7158 161.1173 186.6399 162.1359 218.3264 199.4416 203.8058 176.0004 188.9062 187.4595 233.0947 204.1325 196.8503 194.9761 201.0082
Actin 0.0112 0.0001 0.0004 0.0002 0.0005 0.0002 0.0016 0.0002 0.0016 0.0001 0.0076 0.0006 0.0017 0.0001 0.0003 0.0001 0.0003 0.0008
Bud 0.0011 0.0003 0.0008 0.0011 0.0014 0.0009 0.0011 0.0003 0.0007 0.0007 0.0011 0.0005 0.0005 0.0002 0.0004 0.0005 0.0007 0.0009
Bud Neck 0.0013 0.001 0.0011 0.0013 0.0023 0.0031 0.0006 0.0006 0.0009 0.0011 0.0007 0.0019 0.0006 0.0003 0.0005 0.0008 0.0007 0.0016
Bud Periphery 0.0016 0.0003 0.0011 0.0006 0.0012 0.0007 0.0012 0.0002 0.0006 0.0003 0.0008 0.0004 0.0006 0.0001 0.0003 0.0004 0.0002 0.0008
Bud Site 0.001 0.0003 0.001 0.0003 0.0006 0.0002 0.0006 0.0003 0.0054 0.0002 0.0004 0.0001 0.0002 0.0004 0.0002 0.0001 0.0001 0.0003
Cell Periphery 0.0575 0.0308 0.039 0.0261 0.0156 0.0168 0.0257 0.0097 0.0246 0.0355 0.0097 0.0033 0.0191 0.0082 0.0077 0.0142 0.0039 0.0062
Cytoplasm 0.598 0.863 0.7334 0.7515 0.651 0.7269 0.7818 0.9419 0.8699 0.822 0.829 0.8768 0.8602 0.9573 0.9259 0.7964 0.9334 0.9176
Cytoplasmic Foci 0.0105 0.0002 0.002 0.0004 0.0103 0.0013 0.0062 0.0004 0.0012 0.0007 0.007 0.0021 0.0007 0.0003 0.0007 0.0008 0.0019 0.0012
Eisosomes 0.0004 0 0.0001 0 0.0004 0 0.0007 0 0.0002 0 0.0001 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0386 0.0074 0.015 0.0076 0.0119 0.0453 0.0356 0.0134 0.0144 0.0137 0.0063 0.0254 0.0289 0.0099 0.0115 0.0062 0.0041 0.02
Endosome 0.0041 0.0004 0.0022 0.0013 0.004 0.0027 0.008 0.0004 0.0026 0.0011 0.0073 0.0074 0.0016 0.0003 0.0011 0.002 0.0038 0.0024
Golgi 0.0018 0 0.0003 0 0.0005 0.0002 0.0011 0.0001 0.0003 0.0001 0.0022 0.0029 0.0003 0.0001 0.0001 0.0001 0.0002 0.0003
Lipid Particles 0.0046 0.0007 0.0027 0.0006 0.0068 0.0009 0.0049 0.0005 0.002 0.0017 0.011 0.0033 0.0013 0.0004 0.0006 0.0015 0.001 0.0005
Mitochondria 0.0048 0.0001 0.0033 0.0002 0.0156 0.0011 0.008 0.0002 0.0015 0.0002 0.0223 0.0055 0.0008 0.0001 0.0003 0.0001 0.0003 0.001
None 0.0055 0.0002 0.0012 0.0002 0.0136 0.0004 0.0084 0.0002 0.0018 0.0004 0.0077 0.0012 0.0006 0.0001 0.0004 0.0002 0.0003 0.0006
Nuclear Periphery 0.0441 0.0105 0.0155 0.0089 0.0132 0.0312 0.0222 0.0028 0.007 0.0121 0.0089 0.0082 0.0202 0.0037 0.0061 0.0066 0.0064 0.0065
Nucleolus 0.0123 0.0004 0.0021 0.0008 0.0435 0.0019 0.0111 0.0002 0.0025 0.0019 0.0061 0.0006 0.0004 0.0003 0.0003 0.0003 0.0004 0.001
Nucleus 0.019 0.0047 0.0075 0.0069 0.0225 0.0141 0.0141 0.0026 0.0045 0.0072 0.0069 0.0041 0.0055 0.0015 0.0038 0.0041 0.0041 0.0057
Peroxisomes 0.0005 0 0.0005 0 0.0006 0.0001 0.0004 0 0.0001 0 0.0007 0.0002 0.0009 0 0 0 0.0001 0
Punctate Nuclear 0.0032 0 0.0017 0 0.0103 0.0001 0.0012 0 0.0002 0.0001 0.0005 0.0001 0.0001 0 0 0 0.0002 0.0002
Vacuole 0.1562 0.0752 0.1483 0.1725 0.1342 0.1218 0.0567 0.0245 0.0529 0.0933 0.047 0.0446 0.0495 0.0156 0.0348 0.1437 0.031 0.0273
Vacuole Periphery 0.0224 0.0042 0.0209 0.0195 0.0399 0.0302 0.0086 0.0014 0.005 0.0079 0.0165 0.0106 0.0065 0.0009 0.0048 0.0216 0.007 0.0051

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 485.4384 574.055 685.7557 652.3291 598.019 511.5861 690.9597 714.6859 764.0206 606.8123
Translational Efficiency 1.3608 1.3984 1.2396 1.3899 1.1586 1.2775 1.3632 1.0691 1.1166 1.1908

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Alpha subunit of heterooctameric phosphofructokinase; involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-1

Pfk1

Pfk1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pfk1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available