Standard name
Human Ortholog
Description Enolase I, a phosphopyruvate hydratase; catalyzes conversion of 2-phosphoglycerate to phosphoenolpyruvate during glycolysis and the reverse reaction during gluconeogenesis; expression repressed in response to glucose; protein abundance increases in response to DNA replication stress; N-terminally propionylated in vivo; ENO1 has a paralog, ENO2, that arose from the whole genome duplication
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0.05 0 0 0.05 0.05 0.07 0.08 0.08 0.07 0.08 0 0 0 0.05 0 0 0 0 0 0 0 0
Cytoplasm 0.99 1.0 0.99 1.0 0.95 0.94 0.88 0.76 0.78 0.77 0.79 0.75 1.0 0.99 0.99 1.0 0.97 0.99 0.95 0.88 0.93 0.9 0.89 0.86
Endoplasmic Reticulum 0 0 0 0 0 0 0.1 0.18 0.17 0.17 0.12 0.17 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 2 2 1 6 3 2 0 0 0 0 0 0 0 0 0 0 1 0
Bud 3 0 1 0 6 2 2 20 12 10 16 13 0 1 0 0 3 0 0 1 10 3 11 28
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 0 22 9 12 15 21 37 41 47 30 36 4 9 8 16 13 4 0 0 0 0 0 0
Cytoplasm 145 50 397 313 469 286 384 431 425 461 317 341 124 266 313 326 336 183 140 72 369 65 171 234
Endoplasmic Reticulum 0 0 3 1 7 9 42 100 93 104 47 79 0 2 3 10 10 9 0 0 5 0 0 1
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0
Golgi 0 0 1 0 0 0 1 1 0 1 3 2 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 2 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 1 0 0 2 0 1 1 0 0 0 0 0 1 0 1 1 0 2 2 1
Nuclear Periphery 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Vac/Vac Membrane 1 0 1 0 13 4 3 7 3 5 3 2 0 0 2 0 7 2 0 1 3 0 1 1
Unique Cell Count 146 50 402 313 495 304 436 566 542 595 400 455 124 268 317 327 345 185 148 82 396 73 192 271
Labelled Cell Count 151 50 425 323 508 319 457 601 575 636 421 475 128 278 326 352 371 198 148 82 396 73 192 271


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 41.0 68.9 57.5 59.3 52.3 55.1 61.0 62.9 74.1 82.3 90.1 94.5 90.5 125.5 142.4 95.9 90.0 88.8 89.4 85.4 93.6
Std Deviation (1e-4) 19.9 27.9 26.7 23.1 23.4 26.2 27.3 25.2 28.4 32.4 37.5 37.4 39.5 64.5 71.7 43.7 45.3 51.1 37.3 42.4 44.9
Intensity Change (Log2) – – – 0.05 -0.13 -0.06 0.09 0.13 0.37 0.52 0.65 0.72 0.66 1.13 1.31 0.74 0.65 0.63 0.64 0.57 0.7


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 3.5 2.6 0 3.7 3.0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -1.7 -2.4 -0.3 -0.4 0.7 1.3 1.5 1.2 1.4 -1.0 -1.3 -2.0 -0.4 -1.1 -1.8
Cytoplasm 2.0 -3.3 -3.5 -6.1 -9.9 -9.2 -9.5 -8.8 -10.1 1.2 0.6 0 1.4 -1.4 0.2
Endoplasmic Reticulum 0 0 2.3 5.7 8.4 8.2 8.4 6.4 8.3 0 0 0 2.3 2.2 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 2.9 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 98.5695 122.5177 109.5847 103.6064 116.8261 142.9493 86.0756 106.0525 92.3476 97.1337 77.9074 108.7892 107.2362 140.0998 119.7477 119.9863 118.6577 173.9358
Actin 0.0005 0.0006 0.0006 0.0002 0.0001 0.0002 0.0104 0.0003 0.0143 0.0005 0.0049 0.0114 0.0002 0.0002 0.0001 0 0.0002 0.0003
Bud 0.0004 0.0003 0.0004 0.0001 0 0.0002 0.0008 0.0004 0.0013 0.0014 0.0003 0.0001 0.0002 0.0002 0.0002 0.0001 0.0007 0.0003
Bud Neck 0.0003 0.0002 0.0002 0.0003 0.0002 0.0005 0.0004 0.0001 0.0003 0.0002 0.0003 0.0004 0.0001 0.0001 0.0001 0.0001 0.0001 0.0007
Bud Periphery 0.0001 0 0.0001 0 0 0 0.0004 0.0001 0.0009 0.001 0.0003 0.0002 0 0 0 0 0.0001 0.0001
Bud Site 0.0003 0.0003 0.0001 0 0 0 0.0008 0.0002 0.0013 0.0001 0.0005 0.0001 0.0001 0.0001 0 0 0 0
Cell Periphery 0.0007 0.0006 0.0001 0 0 0 0.0005 0 0.0005 0.0001 0.0004 0 0.0003 0 0 0 0.0002 0
Cytoplasm 0.9468 0.9905 0.9897 0.9928 0.9959 0.991 0.9209 0.9867 0.9385 0.9789 0.8611 0.9612 0.9801 0.9938 0.9963 0.9937 0.9747 0.988
Cytoplasmic Foci 0.0047 0.0008 0.0006 0.0002 0.0001 0.0006 0.0103 0.0086 0.0069 0.0005 0.0248 0.0008 0.002 0.0032 0.0003 0.0003 0.0021 0.0004
Eisosomes 0.0002 0.0003 0 0 0 0 0.0002 0 0.0002 0 0.0001 0.0001 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0005 0.0001 0.0002 0.0001 0.0001 0.0001 0.0009 0.0001 0.001 0.0008 0.0014 0.0008 0.0003 0 0.0001 0.0001 0.0003 0.0002
Endosome 0.0002 0 0.0002 0.0001 0 0.0001 0.0057 0.0002 0.0051 0.0011 0.0287 0.0079 0.0002 0.0001 0.0001 0.0001 0.0002 0.0003
Golgi 0 0 0 0 0 0 0.0029 0 0.0027 0.0001 0.0074 0.0086 0.0001 0 0 0 0 0
Lipid Particles 0.0004 0 0.0001 0 0 0 0.0057 0.0001 0.0007 0 0.0258 0.0012 0.0004 0.0001 0 0 0.0001 0
Mitochondria 0.0031 0.0005 0.0011 0 0 0.0001 0.0037 0.0002 0.006 0.0052 0.004 0.0031 0.0003 0 0 0 0.0004 0.0011
None 0.0025 0.001 0.0008 0.0002 0.0001 0.0003 0.0078 0.0006 0.0053 0.0005 0.0148 0.0002 0.0036 0.0004 0.0003 0.0003 0.0057 0.0003
Nuclear Periphery 0.0059 0.0009 0.0007 0.0012 0.0006 0.0012 0.0041 0.0003 0.0028 0.0014 0.0066 0.0008 0.0021 0.0002 0.0005 0.0008 0.0015 0.002
Nucleolus 0.0101 0.0003 0.0004 0 0 0 0.0064 0.0001 0.0012 0.0001 0.001 0 0.0016 0.0001 0 0 0.0018 0
Nucleus 0.0188 0.002 0.0033 0.0037 0.0025 0.0047 0.0103 0.0014 0.003 0.0038 0.0037 0.0022 0.0061 0.001 0.0015 0.0037 0.0078 0.0049
Peroxisomes 0.0002 0 0 0 0 0 0.0016 0.0001 0.0008 0.0001 0.0031 0.0001 0 0 0 0 0 0
Punctate Nuclear 0.0011 0.0001 0.0001 0 0 0.0002 0.0016 0.0002 0.0007 0.0001 0.0054 0.0001 0.0004 0.0001 0.0001 0.0001 0.0005 0.0001
Vacuole 0.0022 0.0012 0.0011 0.0007 0.0002 0.0006 0.003 0.0003 0.0037 0.0026 0.004 0.0003 0.0013 0.0003 0.0002 0.0006 0.0026 0.0006
Vacuole Periphery 0.0009 0.0001 0.0004 0.0002 0.0001 0.0002 0.0014 0.0001 0.0029 0.0016 0.0014 0.0003 0.0005 0 0.0001 0.0001 0.0009 0.0006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 4715.9204 5453.5943 6952.2559 4119.2614 5620.1063 5458.5765 4684.3485 4535.6632 6460.4376 4067.2209
Translational Efficiency 1.0509 0.9899 0.8126 1.4938 0.9981 0.8893 1.2288 1.4388 1.0375 1.3417

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Enolase I, a phosphopyruvate hydratase; catalyzes conversion of 2-phosphoglycerate to phosphoenolpyruvate during glycolysis and the reverse reaction during gluconeogenesis; expression repressed in response to glucose; protein abundance increases in response to DNA replication stress; N-terminally propionylated in vivo; ENO1 has a paralog, ENO2, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-2

Eno1

Eno1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Eno1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available