Standard name
Human Ortholog
Description High affinity nicotinic acid plasma membrane permease; responsible for uptake of low levels of nicotinic acid; expression of the gene increases in the absence of extracellular nicotinic acid or para-aminobenzoate (PABA)
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 – 0.06 0.1 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud 0 – 0 0 0 0 0 0 – 0 0.06 0 0 0 0 – – – 0 0 0 0.05 0.08 0.09
Bud Neck 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 – 0 0.05 0.05 0 0 0 – 0 0 0 0 0.05 0.07 – – – 0 0 0 0 0 0
Cytoplasm 0.26 – 0.09 0.23 0.35 0.63 0.79 0.8 – 0.76 0.67 0.66 0.68 0.64 0.63 – – – 0.09 0.07 0 0.27 0.28 0.32
Endoplasmic Reticulum 0.62 – 0.74 0.58 0.47 0.19 0 0 – 0 0 0 0.26 0.33 0.26 – – – 0.8 0.83 0.88 0.47 0.44 0.35
Endosome 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Golgi 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Mitochondria 0 – 0 0.07 0 0.13 0.11 0.19 – 0.36 0.48 0.47 0 0 0 – – – 0 0 0 0.1 0.06 0.06
Nucleus 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nuclear Periphery 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0.15 – 0.16 0.12 0.18 0.1 0.09 0 – 0 0 0.05 0.06 0 0.08 – – – 0 0 0 0 0.05 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 – 16 20 13 2 1 1 – 0 0 0 1 2 6 – – – 2 0 6 5 21 15
Bud 0 – 1 2 3 7 12 11 – 10 22 19 2 1 0 – – – 2 0 4 14 39 45
Bud Neck 0 – 0 0 0 0 2 0 – 2 4 2 1 4 5 – – – 0 1 1 6 5 12
Bud Site 0 – 0 0 0 0 0 0 – 1 0 1 0 0 1 – – – – – – – – –
Cell Periphery 0 – 11 11 18 2 5 2 – 2 4 2 4 14 23 – – – 0 0 2 2 8 9
Cytoplasm 9 – 24 47 117 205 404 579 – 408 239 351 108 190 210 – – – 9 2 3 80 138 168
Endoplasmic Reticulum 21 – 196 118 156 62 17 13 – 6 3 9 41 97 87 – – – 80 33 261 142 217 184
Endosome 0 – 3 6 12 8 16 11 – 2 0 1 0 0 0 – – – 0 0 1 1 1 1
Golgi 0 – 11 3 0 0 1 0 – 0 0 0 1 0 0 – – – 0 0 1 0 1 1
Mitochondria 0 – 8 14 10 41 56 134 – 192 171 248 1 0 0 – – – 0 0 3 29 28 30
Nucleus 0 – 0 1 0 0 1 8 – 1 3 3 0 2 2 – – – 0 0 0 0 0 0
Nuclear Periphery 0 – 4 3 3 6 1 2 – 0 1 8 0 1 0 – – – 1 0 5 2 0 1
Nucleolus 0 – 0 0 0 0 0 4 – 0 0 1 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 – 0 0 0 0 0 0 – 1 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 – 0 0 0 1 2 2 – 5 5 12 0 1 6 – – – 0 0 0 1 5 9
Vac/Vac Membrane 5 – 43 25 61 31 46 27 – 21 11 27 10 12 28 – – – 1 1 3 10 22 35
Unique Cell Count 34 265 202 334 325 509 721 537 359 533 159 298 332 101 40 298 301 496 521
Labelled Cell Count 35 317 250 393 365 564 794 651 463 684 169 324 368 101 40 298 301 496 521


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.4 – 12.7 12.3 8.7 5.4 4.2 4.1 – 3.2 3.3 3.1 7.0 7.9 8.7 3.1 3.6 3.9 7.8 7.9 7.4
Std Deviation (1e-4) 3.7 – 4.8 5.7 3.0 1.7 1.5 1.3 – 1.2 1.3 1.0 2.5 3.1 4.4 0.7 1.0 0.9 3.5 2.6 2.6
Intensity Change (Log2) – – – -0.04 -0.54 -1.25 -1.58 -1.63 – -1.99 -1.93 -2.04 -0.87 -0.7 -0.55 -2.03 -1.8 -1.72 -0.7 -0.69 -0.78


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 1.6 -1.2 -3.8 0 0 – 0 0 0 -2.7 -3.6 -2.7 – – –
Bud 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Cell Periphery 0.7 0.7 -2.9 0 0 – 0 0 0 -0.9 0.3 1.5 – – –
Cytoplasm 4.2 7.4 13.4 18.7 20.3 – 17.9 14.4 15.1 12.7 13.3 13.5 – – –
Endoplasmic Reticulum -3.5 -6.7 -13.4 0 0 – 0 0 0 -9.7 -9.8 -11.6 – – –
Endosome 0 1.9 0 0 0 – 0 0 0 0 0 0 – – –
Golgi -1.7 0 0 0 0 – 0 0 0 0 -3.6 0 – – –
Mitochondria 2.0 0 4.2 0 0 – 0 0 0 0 0 0 – – –
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 – – –
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Vacuole -1.2 0.7 -2.4 -3.0 -6.8 – -6.1 -5.8 -5.2 -3.0 -4.9 -2.9 – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0006 0.0017 0.0033 0.0003 0.0012 0.0016 0.0175 0.0007 0.007 0.007 0.002 0.0003 0.0001 0.0002 0.0328 0.0001 0.0104 0.0026
Bud 0.0006 0.0148 0.0117 0 0.0001 0.001 0.0012 0.0005 0.0037 0.0002 0 0 0 0 0.0009 0 0.0002 0.0001
Bud Neck 0.0003 0.0008 0.0007 0.0007 0.006 0.0023 0.0093 0.0003 0.0005 0.0025 0.0008 0.0004 0 0 0.0013 0 0.001 0.0004
Bud Periphery 0.0044 0.007 0.007 0 0.0001 0.0041 0.0029 0.0009 0.0072 0.0005 0 0 0 0 0.0031 0 0.0003 0.0004
Bud Site 0.0009 0.0343 0.0029 0.0002 0.0001 0.0002 0.0042 0.0085 0.003 0.0003 0.0001 0 0 0.0002 0.0142 0 0.0024 0
Cell Periphery 0.0066 0.0069 0.0018 0.0004 0.0009 0.0025 0.0016 0.0127 0.0031 0.0011 0.0003 0.0004 0.0002 0.0006 0.0008 0.0002 0.0004 0.0004
Cytoplasm 0.0789 0.2058 0.095 0.1346 0.3685 0.136 0.0482 0.1024 0.1019 0.0633 0.1775 0.122 0.0206 0.1474 0.1573 0.1082 0.57 0.1672
Cytoplasmic Foci 0.0147 0.0084 0.0061 0.0057 0.0077 0.0088 0.0445 0.002 0.0185 0.0204 0.0112 0.0077 0.0028 0.0032 0.0084 0.002 0.0523 0.0038
Eisosomes 0.0001 0.0002 0.0001 0 0 0.0003 0.0003 0 0.0001 0.0006 0 0.0001 0 0.0001 0.0005 0 0.0002 0
Endoplasmic Reticulum 0.6979 0.5333 0.6524 0.8039 0.5515 0.7113 0.6469 0.7717 0.702 0.6717 0.6887 0.7625 0.8835 0.5523 0.4614 0.5638 0.0775 0.6163
Endosome 0.0138 0.0062 0.0103 0.0114 0.0146 0.0098 0.0421 0.0034 0.007 0.0365 0.0094 0.0089 0.0231 0.0101 0.0096 0.0028 0.0143 0.0058
Golgi 0.0005 0.0004 0.0011 0.0005 0.0013 0.0007 0.0178 0.0003 0.0018 0.0095 0.0078 0.0005 0.0006 0.0003 0.0012 0.0002 0.023 0.0004
Lipid Particles 0.0038 0.0032 0.0069 0.0017 0.0023 0.0046 0.0191 0.0096 0.0096 0.034 0.0246 0.009 0.017 0.0027 0.0056 0.0006 0.033 0.0014
Mitochondria 0.0011 0.0008 0.0019 0.0001 0.0008 0.0006 0.0069 0.0002 0.0013 0.0022 0.0005 0.0001 0 0.0001 0.0033 0.0001 0.043 0.0002
None 0.1147 0.1006 0.1172 0.0166 0.0085 0.0663 0.0753 0.0507 0.0955 0.1205 0.0517 0.0769 0.0011 0.2703 0.2533 0.3148 0.0085 0.1847
Nuclear Periphery 0.0279 0.0352 0.0347 0.0152 0.0225 0.0289 0.0203 0.0195 0.014 0.0107 0.0058 0.0068 0.043 0.007 0.0144 0.0047 0.0017 0.011
Nucleolus 0.0006 0.0024 0.0014 0.0001 0.0001 0.0002 0.001 0.0002 0.0006 0.0003 0 0 0 0 0.0012 0 0.0002 0
Nucleus 0.0125 0.0155 0.021 0.002 0.0034 0.0121 0.0028 0.0035 0.0037 0.0017 0.0015 0.0008 0.0011 0.001 0.0249 0.0005 0.0015 0.0014
Peroxisomes 0.0001 0.0002 0.0002 0 0.0001 0.0001 0.0057 0.0001 0.007 0.0048 0.0153 0.0001 0 0 0.0005 0 0.1575 0
Punctate Nuclear 0.0064 0.0071 0.0093 0.0007 0.0006 0.0017 0.0189 0.0013 0.0046 0.0018 0.0007 0.0004 0.0006 0.0004 0.002 0.0002 0.0006 0.0003
Vacuole 0.0122 0.0143 0.0125 0.0053 0.0086 0.0057 0.0106 0.0109 0.0061 0.009 0.0018 0.0028 0.0054 0.0037 0.0028 0.0013 0.0018 0.0031
Vacuole Periphery 0.0013 0.0011 0.0024 0.0005 0.0012 0.0012 0.0028 0.0007 0.0016 0.0015 0.0002 0.0002 0.0007 0.0003 0.0006 0.0002 0.0003 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 51.2172 51.0089 55.6361 56.0817 37.4366 47.4098 53.7499 41.9393 51.5556 49.0864
Translational Efficiency 0.9609 0.9725 0.7708 0.8145 1.0323 1.1324 1.0562 1.0785 0.8939 0.7962

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description High affinity nicotinic acid plasma membrane permease; responsible for uptake of low levels of nicotinic acid; expression of the gene increases in the absence of extracellular nicotinic acid or para-aminobenzoate (PABA)
Localization
Cell Percentages ER (98%)
Cell Cycle Regulation No
Subcompartmental Group ER-5

Tna1

Tna1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tna1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available