Standard name
Human Ortholog
Description Beta3-like subunit of the yeast AP-3 complex; functions in transport of alkaline phosphatase to the vacuole via the alternate pathway; exists in both cytosolic and peripherally associated membrane-bound pools

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.12 0.16 0.07 0 0 0 0 0 0 0 0.05 0 0.14 0.14 0.1 0 0.07 0.1 0.05 0.08 0.09 0.05 0.06 0.08
Bud 0 0 0 0 0 0.05 0.05 0 0 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.06 0 0 0 0 0 0 0 0.07 0.07 0.1 0.16 0.1 0.1 0 0 0 0 0 0
Endoplasmic Reticulum 0.05 0.06 0 0 0 0 0 0.05 0 0 0 0 0.17 0.17 0.11 0.05 0 0 0 0 0 0 0 0
Endosome 0.14 0.07 0.22 0.16 0.17 0.06 0.18 0.16 0.11 0.11 0.09 0.15 0.19 0.18 0.14 0.37 0.39 0.26 0.16 0.15 0.18 0.14 0.13 0.11
Golgi 0.68 0.64 0.49 0.2 0.29 0.07 0 0.05 0 0 0 0.08 0.47 0.53 0.56 0.29 0.34 0.49 0.73 0.7 0.64 0.69 0.7 0.71
Mitochondria 0.44 0.62 0.43 0.74 0.59 0.85 0.75 0.74 0.81 0.79 0.83 0.75 0.12 0.12 0 0.08 0.1 0.12 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0.07 0.06 0.08 0 0.07 0.05 0.09 0.06 0.06 0.09 0 0 0 0 0 0 0 0 0.07 0.06 0.06 0.06
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.13 0.08 0 0 0 0 0 0
Vac/Vac Membrane 0.06 0.06 0 0 0 0 0 0 0 0 0 0 0.13 0.14 0.15 0.05 0.07 0.05 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 42 40 30 6 6 5 13 27 16 19 20 12 14 14 12 19 19 32 20 20 45 10 18 26
Bud 3 5 9 1 6 20 26 23 22 24 34 15 1 1 1 14 3 5 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 4 4 5 3 2 0 0 0 0 0 0
Cell Periphery 2 1 2 0 0 1 0 3 1 5 2 2 0 0 0 3 1 2 0 0 0 0 0 0
Cytoplasm 4 10 10 6 20 12 16 27 11 10 6 5 7 7 12 72 26 31 0 0 0 1 0 0
Endoplasmic Reticulum 19 15 9 2 6 17 12 32 17 14 7 15 17 17 14 22 11 8 1 2 1 2 3 3
Endosome 50 17 92 39 60 26 85 100 52 62 37 52 19 18 18 162 103 80 65 41 87 28 35 39
Golgi 237 163 201 47 99 28 21 35 11 24 16 28 47 54 70 126 89 149 299 195 309 142 197 244
Mitochondria 151 158 175 175 205 364 363 469 397 441 340 262 12 12 5 35 26 37 3 7 6 5 8 5
Nucleus 1 0 0 1 1 1 0 3 1 0 1 1 0 0 0 0 1 0 0 0 0 0 0 0
Nuclear Periphery 0 1 2 3 3 6 3 8 6 5 4 6 0 0 0 0 1 4 1 0 0 0 0 0
Nucleolus 4 2 3 8 7 8 13 23 9 15 8 10 1 1 1 3 1 2 0 0 0 0 0 0
Peroxisomes 0 2 30 14 28 8 36 32 45 36 26 31 1 0 0 9 9 5 10 4 32 12 17 19
SpindlePole 2 2 12 2 3 2 6 13 12 9 6 10 4 4 2 41 33 25 1 0 0 0 0 0
Vac/Vac Membrane 22 16 17 1 13 8 6 9 6 12 4 6 13 14 19 21 17 15 3 4 0 0 0 2
Unique Cell Count 347 255 409 238 345 430 483 638 490 555 412 350 101 101 126 440 261 307 411 278 488 205 284 346
Labelled Cell Count 537 432 592 305 457 506 600 808 610 681 514 457 136 142 154 527 340 395 411 278 488 205 284 346


Early Golgi, Golgi, Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.1 11.7 9.9 9.0 8.9 7.6 7.6 7.6 7.3 7.3 7.1 7.5 10.5 10.5 11.0 7.8 8.6 10.0 11.2 11.9 12.6
Std Deviation (1e-4) 1.5 1.6 1.4 1.6 1.8 1.4 1.7 1.7 1.4 1.7 1.6 1.7 1.4 1.4 1.4 1.2 1.5 1.7 1.9 2.1 2.2
Intensity Change (Log2) -0.14 -0.16 -0.4 -0.4 -0.39 -0.45 -0.44 -0.48 -0.4 0.08 0.08 0.14 -0.35 -0.21 0.01 0.17 0.26 0.34


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -2.6 -3.6 -4.5 -3.2 -2.2 -2.8 -2.7 -1.5 -2.3 2.1 2.1 0.8 -1.9 0 1.5
Bud 0 -0.5 1.9 2.4 1.3 1.9 1.8 3.9 1.6 0 0 0 0.9 0 -0.5
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.1 2.3 0.3 0.8 1.5 -0.2 -0.7 -1.0 -1.0 0 0 3.5 6.9 4.2 4.4
Endoplasmic Reticulum 0 -0.5 1.5 0.3 2.3 1.1 0.3 -0.5 1.6 6.0 6.0 4.3 2.2 1.5 0.4
Endosome -1.9 -1.7 -6.8 -1.8 -2.8 -4.8 -4.7 -5.3 -2.7 -0.8 -1.0 -2.0 4.6 4.7 1.1
Golgi -7.4 -5.7 -13.9 -15.4 -16.5 -16.5 -16.3 -14.7 -12.3 -0.5 0.8 1.3 -6.1 -3.8 -0.2
Mitochondria 7.6 4.6 12.6 9.8 10.0 11.9 11.7 11.8 8.9 -5.8 -5.8 -8.1 -11.8 -9.0 -8.9
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 1.4 2.2 2.9 1.4 2.2 1.5 2.2 0 0 0 0 0 0
Peroxisomes -0.7 0.4 -3.8 0.1 -1.6 1.0 -0.5 -0.6 0.8 -2.4 -2.8 -3.1 -3.7 -2.1 -3.5
SpindlePole -1.8 -2.0 -2.8 -1.8 -0.9 -0.4 -1.4 -1.4 -0.1 0 0 0 3.8 4.9 3.1
Vacuole -2.8 -0.3 -2.0 -2.7 -2.8 -2.8 -1.8 -2.9 -2.0 3.3 3.7 4.3 0.4 1.4 0.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.2919 6.7047 6.4852 6.227 5.2571 6.3086 7.5376 8.8978 8.184 7.5625 7.9911 8.1265 9.0973 9.8172 9.239 8.9904 8.6611 9.31
Actin 0.0294 0.0076 0.0244 0.0131 0.0479 0.0116 0.0463 0.0185 0.0287 0.016 0.0041 0.0182 0.0106 0.0293 0.0086 0.0028 0.0021 0.0253
Bud 0.0004 0.0006 0.0004 0.0002 0.0004 0.0001 0.0004 0.0006 0.0004 0 0.0001 0.0001 0.0002 0.0003 0.0001 0.0001 0 0.0001
Bud Neck 0.0008 0.0005 0.0005 0.0002 0.0015 0.0006 0.0076 0.0006 0.0004 0.0002 0.0001 0.0003 0.0003 0.0002 0.0002 0.0001 0.0002 0.0003
Bud Periphery 0.0003 0.0002 0.0002 0.0001 0.0003 0.0001 0.0005 0.0002 0.0005 0 0 0.0001 0.0001 0.0001 0 0 0 0.0001
Bud Site 0.0053 0.0099 0.0009 0.0003 0.0009 0.0002 0.0054 0.0142 0.0025 0.0001 0.0001 0.0002 0.0022 0.0008 0.0002 0.0001 0.0001 0.0001
Cell Periphery 0.0001 0.0001 0.0001 0 0.0001 0 0.0003 0 0.0001 0 0 0 0 0 0 0 0 0
Cytoplasm 0.0045 0.0317 0.0044 0.0041 0.0037 0.0029 0.0078 0.0081 0.0026 0.0017 0.0017 0.0035 0.0041 0.005 0.0033 0.0046 0.0042 0.0025
Cytoplasmic Foci 0.128 0.1475 0.1268 0.0941 0.1321 0.1012 0.1155 0.1664 0.1411 0.1247 0.147 0.0901 0.1644 0.1541 0.1464 0.1249 0.1439 0.1183
Eisosomes 0.0001 0 0.0001 0 0.0003 0 0.0007 0 0.0001 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0023 0.0014 0.0007 0.0009 0.001 0.0008 0.0024 0.0004 0.0008 0.0002 0.0002 0.0004 0.0007 0.0019 0.0003 0.0003 0.0004 0.0007
Endosome 0.2773 0.3084 0.2761 0.4432 0.3638 0.2351 0.2576 0.2292 0.2286 0.3085 0.2304 0.1571 0.2218 0.2427 0.2166 0.2836 0.2286 0.1791
Golgi 0.4659 0.4632 0.5283 0.4151 0.4046 0.615 0.4633 0.5234 0.5476 0.5325 0.5929 0.7086 0.5779 0.5434 0.6046 0.5726 0.5986 0.6449
Lipid Particles 0.0381 0.0051 0.0058 0.0034 0.0115 0.0078 0.0072 0.0024 0.0036 0.002 0.0041 0.0025 0.0038 0.0018 0.0022 0.002 0.0028 0.0025
Mitochondria 0.0078 0.0035 0.0074 0.0118 0.0043 0.0122 0.0041 0.0023 0.0034 0.0015 0.0012 0.0094 0.0018 0.0016 0.0026 0.0021 0.0014 0.0037
None 0.0019 0.0002 0.003 0 0.0007 0.0001 0.0082 0.0001 0.0145 0 0 0 0.0001 0.0002 0 0 0 0
Nuclear Periphery 0.0045 0.0003 0.0011 0.0002 0.0011 0.0002 0.0269 0.0001 0.0006 0.0001 0 0.0001 0.0003 0.0002 0 0.0001 0.0001 0.0001
Nucleolus 0.0003 0.0001 0.0005 0 0.0001 0 0.0017 0 0.0001 0 0 0 0 0 0 0 0 0
Nucleus 0.0007 0.0002 0.0014 0 0.0002 0.0001 0.0139 0 0.0003 0 0 0 0.0001 0 0 0 0 0
Peroxisomes 0.0189 0.0138 0.0129 0.0083 0.0198 0.0085 0.0104 0.0321 0.0223 0.0076 0.017 0.0082 0.0104 0.0146 0.0135 0.0041 0.0162 0.0206
Punctate Nuclear 0.0069 0.0003 0.002 0.0001 0.0016 0.0001 0.0172 0.0002 0.0004 0.0001 0.0001 0.0001 0.0002 0.0026 0.0001 0.0001 0.0001 0.0001
Vacuole 0.004 0.0037 0.0015 0.0017 0.0022 0.0013 0.0011 0.0006 0.0007 0.0033 0.0003 0.0004 0.0005 0.0005 0.0004 0.001 0.0005 0.0005
Vacuole Periphery 0.0025 0.0016 0.0016 0.0031 0.0021 0.0018 0.0016 0.0006 0.0007 0.0016 0.0007 0.0007 0.0006 0.0007 0.0007 0.0015 0.0009 0.001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 69.4736 45.4626 40.9642 51.5645 47.0983 58.0895 46.5828 44.7366 50.3915 55.5724
Translational Efficiency 0.5956 0.6363 0.6928 0.5305 0.5699 0.618 0.6264 0.5942 0.5881 0.5771

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Beta3-like subunit of the yeast AP-3 complex; functions in transport of alkaline phosphatase to the vacuole via the alternate pathway; exists in both cytosolic and peripherally associated membrane-bound pools
Localization
Cell Percentages Golgi (11%), endosome (10%), mixed (30%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Apl6

Apl6


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Apl6-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available