Standard name | |
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Human Ortholog | |
Description | Beta3-like subunit of the yeast AP-3 complex; functions in transport of alkaline phosphatase to the vacuole via the alternate pathway; exists in both cytosolic and peripherally associated membrane-bound pools |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 42 | 40 | 30 | 6 | 6 | 5 | 13 | 27 | 16 | 19 | 20 | 12 | 14 | 14 | 12 | 19 | 19 | 32 | 20 | 20 | 45 | 10 | 18 | 26 |
Bud | 3 | 5 | 9 | 1 | 6 | 20 | 26 | 23 | 22 | 24 | 34 | 15 | 1 | 1 | 1 | 14 | 3 | 5 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 4 | 5 | 3 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 1 | 2 | 0 | 0 | 1 | 0 | 3 | 1 | 5 | 2 | 2 | 0 | 0 | 0 | 3 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 4 | 10 | 10 | 6 | 20 | 12 | 16 | 27 | 11 | 10 | 6 | 5 | 7 | 7 | 12 | 72 | 26 | 31 | 0 | 0 | 0 | 1 | 0 | 0 |
Endoplasmic Reticulum | 19 | 15 | 9 | 2 | 6 | 17 | 12 | 32 | 17 | 14 | 7 | 15 | 17 | 17 | 14 | 22 | 11 | 8 | 1 | 2 | 1 | 2 | 3 | 3 |
Endosome | 50 | 17 | 92 | 39 | 60 | 26 | 85 | 100 | 52 | 62 | 37 | 52 | 19 | 18 | 18 | 162 | 103 | 80 | 65 | 41 | 87 | 28 | 35 | 39 |
Golgi | 237 | 163 | 201 | 47 | 99 | 28 | 21 | 35 | 11 | 24 | 16 | 28 | 47 | 54 | 70 | 126 | 89 | 149 | 299 | 195 | 309 | 142 | 197 | 244 |
Mitochondria | 151 | 158 | 175 | 175 | 205 | 364 | 363 | 469 | 397 | 441 | 340 | 262 | 12 | 12 | 5 | 35 | 26 | 37 | 3 | 7 | 6 | 5 | 8 | 5 |
Nucleus | 1 | 0 | 0 | 1 | 1 | 1 | 0 | 3 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 1 | 2 | 3 | 3 | 6 | 3 | 8 | 6 | 5 | 4 | 6 | 0 | 0 | 0 | 0 | 1 | 4 | 1 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 4 | 2 | 3 | 8 | 7 | 8 | 13 | 23 | 9 | 15 | 8 | 10 | 1 | 1 | 1 | 3 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 2 | 30 | 14 | 28 | 8 | 36 | 32 | 45 | 36 | 26 | 31 | 1 | 0 | 0 | 9 | 9 | 5 | 10 | 4 | 32 | 12 | 17 | 19 |
SpindlePole | 2 | 2 | 12 | 2 | 3 | 2 | 6 | 13 | 12 | 9 | 6 | 10 | 4 | 4 | 2 | 41 | 33 | 25 | 1 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 22 | 16 | 17 | 1 | 13 | 8 | 6 | 9 | 6 | 12 | 4 | 6 | 13 | 14 | 19 | 21 | 17 | 15 | 3 | 4 | 0 | 0 | 0 | 2 |
Unique Cell Count | 347 | 255 | 409 | 238 | 345 | 430 | 483 | 638 | 490 | 555 | 412 | 350 | 101 | 101 | 126 | 440 | 261 | 307 | 411 | 278 | 488 | 205 | 284 | 346 |
Labelled Cell Count | 537 | 432 | 592 | 305 | 457 | 506 | 600 | 808 | 610 | 681 | 514 | 457 | 136 | 142 | 154 | 527 | 340 | 395 | 411 | 278 | 488 | 205 | 284 | 346 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 11.1 | 11.7 | 9.9 | 9.0 | 8.9 | 7.6 | 7.6 | 7.6 | 7.3 | 7.3 | 7.1 | 7.5 | 10.5 | 10.5 | 11.0 | 7.8 | 8.6 | 10.0 | 11.2 | 11.9 | 12.6 |
Std Deviation (1e-4) | 1.5 | 1.6 | 1.4 | 1.6 | 1.8 | 1.4 | 1.7 | 1.7 | 1.4 | 1.7 | 1.6 | 1.7 | 1.4 | 1.4 | 1.4 | 1.2 | 1.5 | 1.7 | 1.9 | 2.1 | 2.2 |
Intensity Change (Log2) | – | – | – | -0.14 | -0.16 | -0.4 | -0.4 | -0.39 | -0.45 | -0.44 | -0.48 | -0.4 | 0.08 | 0.08 | 0.14 | -0.35 | -0.21 | 0.01 | 0.17 | 0.26 | 0.34 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -2.6 | -3.6 | -4.5 | -3.2 | -2.2 | -2.8 | -2.7 | -1.5 | -2.3 | 2.1 | 2.1 | 0.8 | -1.9 | 0 | 1.5 |
Bud | 0 | -0.5 | 1.9 | 2.4 | 1.3 | 1.9 | 1.8 | 3.9 | 1.6 | 0 | 0 | 0 | 0.9 | 0 | -0.5 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.1 | 2.3 | 0.3 | 0.8 | 1.5 | -0.2 | -0.7 | -1.0 | -1.0 | 0 | 0 | 3.5 | 6.9 | 4.2 | 4.4 |
Endoplasmic Reticulum | 0 | -0.5 | 1.5 | 0.3 | 2.3 | 1.1 | 0.3 | -0.5 | 1.6 | 6.0 | 6.0 | 4.3 | 2.2 | 1.5 | 0.4 |
Endosome | -1.9 | -1.7 | -6.8 | -1.8 | -2.8 | -4.8 | -4.7 | -5.3 | -2.7 | -0.8 | -1.0 | -2.0 | 4.6 | 4.7 | 1.1 |
Golgi | -7.4 | -5.7 | -13.9 | -15.4 | -16.5 | -16.5 | -16.3 | -14.7 | -12.3 | -0.5 | 0.8 | 1.3 | -6.1 | -3.8 | -0.2 |
Mitochondria | 7.6 | 4.6 | 12.6 | 9.8 | 10.0 | 11.9 | 11.7 | 11.8 | 8.9 | -5.8 | -5.8 | -8.1 | -11.8 | -9.0 | -8.9 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 1.4 | 2.2 | 2.9 | 1.4 | 2.2 | 1.5 | 2.2 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | -0.7 | 0.4 | -3.8 | 0.1 | -1.6 | 1.0 | -0.5 | -0.6 | 0.8 | -2.4 | -2.8 | -3.1 | -3.7 | -2.1 | -3.5 |
SpindlePole | -1.8 | -2.0 | -2.8 | -1.8 | -0.9 | -0.4 | -1.4 | -1.4 | -0.1 | 0 | 0 | 0 | 3.8 | 4.9 | 3.1 |
Vacuole | -2.8 | -0.3 | -2.0 | -2.7 | -2.8 | -2.8 | -1.8 | -2.9 | -2.0 | 3.3 | 3.7 | 4.3 | 0.4 | 1.4 | 0.5 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 5.2919 | 6.7047 | 6.4852 | 6.227 | 5.2571 | 6.3086 | 7.5376 | 8.8978 | 8.184 | 7.5625 | 7.9911 | 8.1265 | 9.0973 | 9.8172 | 9.239 | 8.9904 | 8.6611 | 9.31 |
Actin | 0.0294 | 0.0076 | 0.0244 | 0.0131 | 0.0479 | 0.0116 | 0.0463 | 0.0185 | 0.0287 | 0.016 | 0.0041 | 0.0182 | 0.0106 | 0.0293 | 0.0086 | 0.0028 | 0.0021 | 0.0253 |
Bud | 0.0004 | 0.0006 | 0.0004 | 0.0002 | 0.0004 | 0.0001 | 0.0004 | 0.0006 | 0.0004 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0001 |
Bud Neck | 0.0008 | 0.0005 | 0.0005 | 0.0002 | 0.0015 | 0.0006 | 0.0076 | 0.0006 | 0.0004 | 0.0002 | 0.0001 | 0.0003 | 0.0003 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0003 |
Bud Periphery | 0.0003 | 0.0002 | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0.0005 | 0.0002 | 0.0005 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0001 |
Bud Site | 0.0053 | 0.0099 | 0.0009 | 0.0003 | 0.0009 | 0.0002 | 0.0054 | 0.0142 | 0.0025 | 0.0001 | 0.0001 | 0.0002 | 0.0022 | 0.0008 | 0.0002 | 0.0001 | 0.0001 | 0.0001 |
Cell Periphery | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0003 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.0045 | 0.0317 | 0.0044 | 0.0041 | 0.0037 | 0.0029 | 0.0078 | 0.0081 | 0.0026 | 0.0017 | 0.0017 | 0.0035 | 0.0041 | 0.005 | 0.0033 | 0.0046 | 0.0042 | 0.0025 |
Cytoplasmic Foci | 0.128 | 0.1475 | 0.1268 | 0.0941 | 0.1321 | 0.1012 | 0.1155 | 0.1664 | 0.1411 | 0.1247 | 0.147 | 0.0901 | 0.1644 | 0.1541 | 0.1464 | 0.1249 | 0.1439 | 0.1183 |
Eisosomes | 0.0001 | 0 | 0.0001 | 0 | 0.0003 | 0 | 0.0007 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0023 | 0.0014 | 0.0007 | 0.0009 | 0.001 | 0.0008 | 0.0024 | 0.0004 | 0.0008 | 0.0002 | 0.0002 | 0.0004 | 0.0007 | 0.0019 | 0.0003 | 0.0003 | 0.0004 | 0.0007 |
Endosome | 0.2773 | 0.3084 | 0.2761 | 0.4432 | 0.3638 | 0.2351 | 0.2576 | 0.2292 | 0.2286 | 0.3085 | 0.2304 | 0.1571 | 0.2218 | 0.2427 | 0.2166 | 0.2836 | 0.2286 | 0.1791 |
Golgi | 0.4659 | 0.4632 | 0.5283 | 0.4151 | 0.4046 | 0.615 | 0.4633 | 0.5234 | 0.5476 | 0.5325 | 0.5929 | 0.7086 | 0.5779 | 0.5434 | 0.6046 | 0.5726 | 0.5986 | 0.6449 |
Lipid Particles | 0.0381 | 0.0051 | 0.0058 | 0.0034 | 0.0115 | 0.0078 | 0.0072 | 0.0024 | 0.0036 | 0.002 | 0.0041 | 0.0025 | 0.0038 | 0.0018 | 0.0022 | 0.002 | 0.0028 | 0.0025 |
Mitochondria | 0.0078 | 0.0035 | 0.0074 | 0.0118 | 0.0043 | 0.0122 | 0.0041 | 0.0023 | 0.0034 | 0.0015 | 0.0012 | 0.0094 | 0.0018 | 0.0016 | 0.0026 | 0.0021 | 0.0014 | 0.0037 |
None | 0.0019 | 0.0002 | 0.003 | 0 | 0.0007 | 0.0001 | 0.0082 | 0.0001 | 0.0145 | 0 | 0 | 0 | 0.0001 | 0.0002 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0.0045 | 0.0003 | 0.0011 | 0.0002 | 0.0011 | 0.0002 | 0.0269 | 0.0001 | 0.0006 | 0.0001 | 0 | 0.0001 | 0.0003 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0001 |
Nucleolus | 0.0003 | 0.0001 | 0.0005 | 0 | 0.0001 | 0 | 0.0017 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0.0007 | 0.0002 | 0.0014 | 0 | 0.0002 | 0.0001 | 0.0139 | 0 | 0.0003 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0.0189 | 0.0138 | 0.0129 | 0.0083 | 0.0198 | 0.0085 | 0.0104 | 0.0321 | 0.0223 | 0.0076 | 0.017 | 0.0082 | 0.0104 | 0.0146 | 0.0135 | 0.0041 | 0.0162 | 0.0206 |
Punctate Nuclear | 0.0069 | 0.0003 | 0.002 | 0.0001 | 0.0016 | 0.0001 | 0.0172 | 0.0002 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0026 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Vacuole | 0.004 | 0.0037 | 0.0015 | 0.0017 | 0.0022 | 0.0013 | 0.0011 | 0.0006 | 0.0007 | 0.0033 | 0.0003 | 0.0004 | 0.0005 | 0.0005 | 0.0004 | 0.001 | 0.0005 | 0.0005 |
Vacuole Periphery | 0.0025 | 0.0016 | 0.0016 | 0.0031 | 0.0021 | 0.0018 | 0.0016 | 0.0006 | 0.0007 | 0.0016 | 0.0007 | 0.0007 | 0.0006 | 0.0007 | 0.0007 | 0.0015 | 0.0009 | 0.001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 69.4736 | 45.4626 | 40.9642 | 51.5645 | 47.0983 | 58.0895 | 46.5828 | 44.7366 | 50.3915 | 55.5724 |
Translational Efficiency | 0.5956 | 0.6363 | 0.6928 | 0.5305 | 0.5699 | 0.618 | 0.6264 | 0.5942 | 0.5881 | 0.5771 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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