Standard name
Human Ortholog
Description Protein kinase; component of the EKC/KEOPS complex with Kae1p, Cgi121p, Pcc1p, and Gon7p; Pyrococcus Bud32 ortholog functions as a P-loop ATPase rather than a protein kinase in the context of the complex; EKC/KEOPS complex is required for t6A tRNA modification and telomeric TG1-3 recombination; may have role in transcription; mutation is functionally complemented by human TP53RK

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.05 0.06 0.05 0 0.09 0.06 0 0.06 0 0 0.09 0.13 0.21 0.15 0.13 0.16 0 0 0 0.05 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.19 0 0.07 0 0 0 0 0.27 0.13 0.21 0.15 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.84 0.8 0.88 0.81 0.84 0.87 0.82 0.92 0.78 0.85 0.8 0.78 0.81 0.78 0.75 0.75 0.72 0.68 0.86 0.88 0.83 0.77 0.87 0.76
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0.07 0 0 0 0 0.08 0.07 0.11 0.08 0 0 0 0 0 0 0 0 0 0 0 0.07
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.16 0.12 0.09 0.09 0.1 0.09 0.07 0 0 0 0 0.08 0.15 0.08 0.05 0.12 0.18 0.19 0 0.05 0 0.08 0.06 0.1
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0 1 0 2 1 1 4 4
Bud Neck 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 1 2 1 1 3 8 4 6 5 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Cytoplasm 3 5 3 5 6 7 18 16 8 11 6 8 5 26 43 61 50 58 2 3 1 4 2 2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 18 13 19 0 0 0 1 0 0
Endosome 0 0 0 2 1 0 4 2 1 0 0 0 0 3 2 4 7 1 1 0 0 1 1 0
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 4 1 2 1 0 0 0 0
Mitochondria 2 38 0 6 2 4 6 11 58 25 41 31 1 1 0 10 11 13 0 2 1 0 0 1
Nucleus 67 164 49 71 101 141 165 258 168 165 153 159 44 154 153 301 283 250 87 190 77 68 161 137
Nuclear Periphery 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 3 2 1 6 0 2 1 1 17 14 22 17 0 0 1 0 1 4 0 0 1 2 1 11
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 2 0 0 1 0 0 0 1 2
Vac/Vac Membrane 13 25 5 8 12 14 14 8 8 7 7 16 8 16 10 49 71 69 3 10 3 6 10 19
Unique Cell Count 80 205 56 88 120 162 200 280 215 194 192 203 54 197 204 400 391 367 102 215 94 89 186 182
Labelled Cell Count 89 234 58 100 125 169 209 299 269 227 235 236 58 202 211 445 441 417 102 215 94 89 186 182


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.9 5.8 8.7 7.7 9.2 7.6 7.6 7.7 6.6 7.3 6.7 6.9 7.2 8.6 8.5 6.7 9.0 8.2 9.9 9.7 9.7
Std Deviation (1e-4) 1.9 0.9 1.7 1.6 2.2 1.7 1.8 1.6 1.5 1.7 1.6 1.8 1.5 2.0 2.0 1.6 1.9 2.0 2.7 2.1 1.6
Intensity Change (Log2) -0.16 0.08 -0.19 -0.18 -0.17 -0.4 -0.25 -0.37 -0.32 -0.26 -0.01 -0.03 -0.37 0.05 -0.07 0.19 0.17 0.17


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 1.6 2.7 2.0 1.6 2.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 4.4 2.8 3.8 3.1 0 0 0 0 0 0
Nucleus -1.1 -0.6 -0.1 -0.9 1.1 -1.6 -0.5 -1.3 -1.5 -0.9 -1.5 -2.0 -2.0 -2.4 -3.0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 2.2 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0.2 0 0 0 0 0 0 0 1.0 0 0 0.7 1.7 1.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.034 3.2255 2.9653 1.9922 2.6268 2.7427 5.7404 6.9868 6.1838 5.7754 5.7256 6.3386 2.8415 3.4893 2.8965 3.2593 3.1121 3.182
Actin 0.0229 0 0.0122 0.0001 0.0152 0.0051 0.0122 0 0.0094 0.0162 0.0049 0.0113 0.0374 0.0001 0.0041 0.0015 0.0137 0.0058
Bud 0.0002 0 0.0001 0 0.0008 0.0001 0.0003 0.0001 0.001 0.0003 0 0.0003 0.0009 0.0003 0.0004 0.0001 0.0018 0.0003
Bud Neck 0.001 0.0001 0.0003 0.0002 0.0014 0.0009 0.0029 0.0002 0.0009 0.0009 0.001 0.0018 0.0024 0.0003 0.0018 0.0007 0.0009 0.0018
Bud Periphery 0.0005 0 0.0001 0 0.0017 0.0001 0.0004 0 0.002 0.0007 0.0001 0.0005 0.0012 0.0002 0.0008 0.0001 0.0024 0.0006
Bud Site 0.0005 0.0001 0.0007 0.0001 0.0052 0.0001 0.003 0.0004 0.0043 0.0007 0.0001 0.0005 0.0027 0.0009 0.0027 0.0004 0.004 0.0006
Cell Periphery 0.0001 0 0 0 0.0003 0 0.0002 0 0.0002 0.0001 0 0.0001 0.0001 0 0.0001 0 0.0002 0.0001
Cytoplasm 0.0052 0.0055 0.0193 0.0052 0.0024 0.001 0.0078 0.0291 0.0091 0.0017 0.0046 0.0072 0.0057 0.0198 0.0055 0.0035 0.0061 0.014
Cytoplasmic Foci 0.0017 0.0005 0.0023 0.0289 0.0058 0.0003 0.0059 0.0003 0.0044 0.0015 0.0055 0.0015 0.0169 0.0001 0.004 0.0007 0.0157 0.0005
Eisosomes 0.0003 0 0.0001 0 0.0003 0 0.0002 0 0.0001 0.0002 0 0.0001 0.0002 0 0 0 0.0001 0.0001
Endoplasmic Reticulum 0.0031 0.0002 0.0016 0.0001 0.0052 0.0002 0.0018 0.0006 0.0012 0.0003 0.002 0.0008 0.0013 0.0005 0.001 0.0002 0.0042 0.0074
Endosome 0.009 0.0003 0.0068 0.0002 0.0422 0.0018 0.0068 0.0003 0.0074 0.0028 0.0125 0.0033 0.0155 0.0006 0.0059 0.0052 0.0362 0.0051
Golgi 0.0051 0 0.0012 0.0003 0.0102 0.0014 0.0021 0 0.004 0.0013 0.0028 0.0028 0.0078 0 0.0015 0.0071 0.0208 0.001
Lipid Particles 0.0073 0 0.003 0.0014 0.021 0.0005 0.005 0 0.009 0.0031 0.0271 0.0037 0.015 0 0.0071 0.0131 0.0128 0.001
Mitochondria 0.0116 0.0001 0.0008 0.0002 0.0278 0.0004 0.0028 0.0001 0.0102 0.0101 0.0008 0.0024 0.0057 0.0004 0.0023 0.0101 0.0039 0.0056
None 0.0013 0.0031 0.003 0.0001 0.0015 0.0001 0.0047 0.0035 0.003 0.0012 0.0023 0.0047 0.0019 0.0002 0.0014 0.0002 0.002 0.0167
Nuclear Periphery 0.0216 0.009 0.0167 0.0033 0.0491 0.002 0.0197 0.0035 0.0062 0.0024 0.0181 0.0036 0.0136 0.0079 0.007 0.0032 0.0121 0.0127
Nucleolus 0.0028 0.0021 0.0013 0.0013 0.004 0.004 0.0053 0.0024 0.0044 0.0026 0.0039 0.0039 0.0035 0.0022 0.003 0.0027 0.0033 0.0056
Nucleus 0.896 0.9719 0.9164 0.9257 0.7738 0.9808 0.8985 0.9541 0.9093 0.9442 0.8811 0.9464 0.8396 0.9622 0.941 0.9491 0.8459 0.9162
Peroxisomes 0.0029 0 0.0091 0.0311 0.0128 0.0002 0.0092 0 0.0064 0.0062 0.0084 0.0016 0.016 0 0.0052 0.0005 0.0025 0.0003
Punctate Nuclear 0.0037 0.0065 0.0029 0.0015 0.0052 0.0005 0.0089 0.0051 0.0033 0.0022 0.0234 0.0026 0.0094 0.0025 0.0031 0.0006 0.0049 0.0021
Vacuole 0.0017 0.0004 0.0017 0.0001 0.0059 0.0003 0.0016 0.0003 0.0028 0.001 0.0005 0.0005 0.0023 0.0015 0.0012 0.0004 0.0047 0.0014
Vacuole Periphery 0.0015 0.0001 0.0004 0 0.008 0.0001 0.0008 0 0.0011 0.0005 0.0008 0.0003 0.0008 0.0003 0.0006 0.0006 0.0018 0.0012

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 26.6726 13.8042 17.6931 22.7018 19.1112 11.2244 20.3018 29.1174 24.8102 22.8381
Translational Efficiency 1.3427 2.0965 1.7443 1.0944 1.5088 3.0924 1.5862 0.8212 1.0768 1.4461

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Protein kinase; component of the EKC/KEOPS complex with Kae1p, Cgi121p, Pcc1p, and Gon7p; Pyrococcus Bud32 ortholog functions as a P-loop ATPase rather than a protein kinase in the context of the complex; EKC/KEOPS complex is required for t6A tRNA modification and telomeric TG1-3 recombination; may have role in transcription; mutation is functionally complemented by human TP53RK
Localization
Cell Percentages nucleus (98%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-5

Bud32

Bud32


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Bud32-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available