Standard name
Human Ortholog
Description Methionyl-tRNA synthetase; forms a complex with Gus1p, a glutamyl-tRNA synthetase, and Arc1p, which increases the catalytic efficiency of both synthetases; involved in the nuclear export of tRNAs; mutations in human ortholog MARS is associated with pediatric pulmonary alveolar proteinosis, interstitial lung and liver disease, and Charcot-Marie-Tooth disease; human MARS gene can complement the yeast null mutant
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0.05 0.05 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0.05 0.07 – 0.06 0.09 0.09 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 0.99 0.99 0.97 0.96 0.92 0.83 – 0.79 0.74 0.7 0.77 0.97 0.98 0.98 0.99 1.0 0.97 0.99 0.98 0.96 0.83 0.86 0.85
Endoplasmic Reticulum 0 0 0 0 0 0.07 0.11 – 0.14 0.18 0.22 0.13 0 0 0 0 0 0 0 0 0 0.12 0.1 0.1
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 2 2 – 14 14 9 12 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 1 1 1 0 0 0 0 0 0 0 0 1 3
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 1 7 3 5 12 18 – 16 27 21 19 1 3 3 2 3 7 0 0 0 0 0 0
Cytoplasm 126 182 205 134 182 206 228 – 221 223 163 203 433 567 600 269 161 151 122 186 205 335 418 464
Endoplasmic Reticulum 0 1 2 4 6 15 31 – 39 55 50 34 11 16 17 7 4 7 1 0 3 49 48 57
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 1 – 6 1 2 5 0 0 0 0 0 0 0 0 0 1 2 3
Mitochondria 0 0 0 0 0 0 2 – 8 9 4 5 0 0 0 1 0 0 0 0 0 1 2 1
Nucleus 0 0 0 0 0 0 0 – 0 2 0 0 1 0 0 1 0 0 0 1 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 1 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 1 0 0 0 0 2 – 1 5 1 8 1 0 1 2 0 0 0 0 1 7 8 10
Unique Cell Count 126 183 207 138 190 224 275 280 302 232 263 445 579 610 273 161 156 124 191 213 403 485 549
Labelled Cell Count 127 185 214 141 194 235 284 305 336 250 287 449 587 621 282 168 165 124 191 213 403 485 549


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 68.3 70.2 55.9 50.3 45.7 37.5 33.3 – 28.7 26.8 26.4 25.8 58.4 61.1 57.9 67.0 71.3 66.5 57.6 58.5 60.7
Std Deviation (1e-4) 11.4 14.0 10.6 12.5 9.4 8.4 7.1 – 7.0 7.0 5.9 6.3 11.9 10.2 10.0 15.2 17.6 15.3 10.5 12.6 13.9
Intensity Change (Log2) – – – -0.15 -0.29 -0.58 -0.75 – -0.96 -1.06 -1.08 -1.12 0.06 0.13 0.05 0.26 0.35 0.25 0.04 0.06 0.12


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 3.3 3.1 0 3.1 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 -0.4 1.0 1.6 – 1.2 2.5 2.4 1.8 0 0 0 0 0 0.5
Cytoplasm -1.3 -2.1 -3.5 -5.8 – -6.6 -7.6 -8.2 -6.9 -1.4 -1.0 -0.7 -0.5 1.3 -1.5
Endoplasmic Reticulum 0 0 3.1 4.4 – 5.1 6.1 6.7 4.8 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.004 0.0003 0.0086 0 0.0126 0.0018 0.0267 0.0015 0.0004 0.0038 0.0341 0.0008 0.0114 0.0004 0.0146 0.0234 0.0048 0.0698
Bud 0.0029 0.0006 0.0016 0.0001 0.0024 0.0002 0.0043 0.0009 0.0004 0.0021 0.0032 0.0013 0.0016 0.0002 0.0007 0.0043 0.0023 0.0003
Bud Neck 0.0008 0.0002 0.0074 0 0.0013 0.0006 0.0011 0.0002 0.0003 0.0006 0.0021 0.0008 0.0008 0.0002 0.0003 0.0017 0.0008 0.0013
Bud Periphery 0.0047 0.0004 0.0009 0 0.0018 0 0.0039 0.0007 0.0002 0.0015 0.0019 0.0053 0.0008 0.0001 0.0006 0.0047 0.0047 0.0004
Bud Site 0.0005 0.0012 0.0064 0 0.0011 0 0.0083 0.0018 0.0003 0.0052 0.0025 0 0.0017 0.0004 0.0005 0.0484 0.0035 0.0001
Cell Periphery 0.0035 0.0011 0.001 0 0.0007 0.0001 0.0139 0.0034 0.0016 0.0019 0.0006 0.0011 0.0031 0.0014 0.0006 0.0007 0.0006 0.0001
Cytoplasm 0.082 0.9725 0.8846 0.9932 0.7031 0.9569 0.5348 0.9666 0.9411 0.796 0.7836 0.9332 0.7958 0.977 0.9065 0.8834 0.806 0.906
Cytoplasmic Foci 0.0352 0.0024 0.0292 0.0004 0.014 0.0066 0.045 0.0017 0.0021 0.0221 0.0195 0.0034 0.0221 0.001 0.011 0.0026 0.0078 0.0029
Eisosomes 0 0 0 0 0.0002 0 0.0004 0.0003 0 0.0003 0.0001 0 0.0001 0 0.0001 0.0002 0.0001 0.0003
Endoplasmic Reticulum 0.0023 0.0028 0.0029 0 0.0082 0.0057 0.0582 0.0025 0.0136 0.0017 0.0044 0.0129 0.0629 0.005 0.0011 0.0011 0.0234 0.0031
Endosome 0.0182 0.0006 0.0102 0.0002 0.0809 0.0178 0.0602 0.0034 0.0065 0.0067 0.0285 0.0056 0.0114 0.003 0.0058 0.0029 0.0471 0.0037
Golgi 0.0036 0.0001 0.0032 0 0.0102 0.0043 0.0085 0.001 0.0013 0.0018 0.0189 0.0011 0.0026 0.0017 0.01 0.0022 0.0231 0.0038
Lipid Particles 0.0364 0.0008 0.0028 0 0.0053 0 0.0164 0.0008 0.0004 0.008 0.001 0 0.0034 0.0005 0.0044 0.0018 0.0086 0.0004
Mitochondria 0.0119 0.0016 0.0012 0 0.0116 0.0012 0.0189 0.0016 0.0006 0.0245 0.0603 0.0132 0.0019 0.0003 0.0148 0.0044 0.039 0.0009
None 0.002 0.0019 0.0005 0.0001 0.0048 0.0002 0.0222 0.0003 0.0007 0.0018 0.0012 0.001 0.0058 0.0007 0.004 0.0003 0.0004 0.0004
Nuclear Periphery 0.0262 0.0006 0.0022 0.0003 0.0929 0.0009 0.0637 0.0009 0.0023 0.0014 0.0016 0.0031 0.0271 0.0006 0.0013 0.0017 0.0019 0.0034
Nucleolus 0.0039 0.0013 0.0004 0 0.0024 0 0.0056 0.0003 0.0001 0.0021 0.0004 0 0.0012 0.0002 0.0005 0.0005 0.0005 0
Nucleus 0.2804 0.001 0.0037 0.001 0.021 0.0005 0.0313 0.001 0.0011 0.0026 0.002 0.0016 0.0096 0.0012 0.0012 0.0019 0.0013 0.0008
Peroxisomes 0.2543 0.0002 0.0072 0 0.0012 0.0001 0.003 0.0002 0 0.0029 0.0015 0 0.0042 0.0001 0.0004 0.0015 0.0028 0.0002
Punctate Nuclear 0.0052 0.0001 0.0053 0 0.0085 0 0.0177 0.0002 0.0001 0.0005 0.0002 0 0.0054 0 0.0013 0.0006 0.0004 0.0003
Vacuole 0.1188 0.0088 0.0178 0.004 0.0072 0.0016 0.0417 0.01 0.0249 0.1035 0.0222 0.0087 0.0245 0.0057 0.0165 0.0102 0.0132 0.0013
Vacuole Periphery 0.1035 0.0015 0.0028 0.0006 0.0086 0.0013 0.0143 0.0008 0.002 0.009 0.0101 0.0067 0.0027 0.0004 0.0039 0.0012 0.0075 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 202.9756 173.1405 179.4146 205.3588 181.9972 142.2486 155.6108 159.0321 138.849 191.8232
Translational Efficiency 1.1015 1.2657 1.2905 0.9923 1.0823 1.2217 1.2083 0.8316 1.041 0.9355

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Methionyl-tRNA synthetase; forms a complex with Gus1p, a glutamyl-tRNA synthetase, and Arc1p, which increases the catalytic efficiency of both synthetases; involved in the nuclear export of tRNAs; mutations in human ortholog MARS is associated with pediatric pulmonary alveolar proteinosis, interstitial lung and liver disease, and Charcot-Marie-Tooth disease; human MARS gene can complement the yeast null mutant
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-2

Mes1

Mes1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mes1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available