Standard name | |
---|---|
Human Ortholog | |
Description | Putative RNA helicase related to Ski2p; involved in translation inhibition of non-poly(A) mRNAs; required for repressing propagation of dsRNA viruses |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 1 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud | 1 | 3 | 17 | 3 | 3 | 4 | 5 | 14 | 1 | 7 | 2 | 8 | 4 | 0 | 13 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Bud Site | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 8 | 2 | 2 | 1 | 6 | 4 | 7 | 6 | 2 | 8 | 11 | 11 | 8 | 7 | 9 | 11 | 14 | 17 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 339 | 181 | 258 | 137 | 230 | 136 | 198 | 219 | 115 | 198 | 107 | 180 | 424 | 430 | 460 | 137 | 102 | 139 | 350 | 179 | 247 | 301 | 421 | 443 |
Endoplasmic Reticulum | 8 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 2 | 0 | 1 | 2 | 0 | 1 | 0 | 5 | 3 | 7 | 2 | 0 | 3 | 15 | 20 | 19 |
Endosome | 2 | 1 | 0 | 0 | 3 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 2 | 1 | 0 | 1 | 0 | 1 | 1 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 1 | 1 | 1 |
Mitochondria | 1 | 2 | 20 | 33 | 28 | 43 | 81 | 117 | 70 | 98 | 88 | 143 | 3 | 1 | 22 | 3 | 3 | 2 | 0 | 3 | 18 | 5 | 4 | 8 |
Nucleus | 0 | 3 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 7 | 1 | 0 | 0 | 5 | 1 | 1 | 13 | 0 | 4 | 0 | 3 | 1 | 1 | 2 | 13 | 9 | 7 | 0 | 7 | 6 | 5 | 5 | 7 |
Unique Cell Count | 353 | 186 | 272 | 143 | 244 | 157 | 226 | 285 | 148 | 240 | 157 | 263 | 428 | 433 | 467 | 151 | 116 | 154 | 362 | 194 | 285 | 335 | 459 | 492 |
Labelled Cell Count | 367 | 194 | 299 | 175 | 279 | 189 | 294 | 373 | 190 | 319 | 210 | 349 | 443 | 440 | 506 | 173 | 132 | 177 | 362 | 194 | 285 | 335 | 459 | 492 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 8.4 | 6.2 | 4.5 | 4.5 | 5.0 | 4.1 | 4.0 | 4.1 | 3.6 | 3.6 | 3.6 | 3.4 | 6.9 | 6.8 | 6.0 | 8.7 | 9.8 | 9.1 | 7.1 | 7.7 | 8.0 |
Std Deviation (1e-4) | 1.0 | 1.0 | 0.9 | 1.2 | 1.3 | 0.8 | 1.1 | 1.2 | 1.2 | 1.1 | 1.3 | 1.2 | 0.9 | 0.8 | 0.8 | 1.3 | 2.2 | 2.3 | 1.0 | 1.2 | 1.3 |
Intensity Change (Log2) | – | – | – | 0.0 | 0.14 | -0.15 | -0.16 | -0.13 | -0.33 | -0.34 | -0.34 | -0.42 | 0.61 | 0.58 | 0.41 | 0.95 | 1.11 | 1.01 | 0.64 | 0.76 | 0.83 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | -2.9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -4.0 | -5.3 | -2.3 | -3.1 | -2.8 | -2.4 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.9 |
Cytoplasm | 0.4 | -0.3 | -3.0 | -2.9 | -6.1 | -5.3 | -4.5 | -7.5 | -7.9 | 3.4 | 3.8 | 2.9 | -1.6 | -2.4 | -1.8 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 1.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -4.8 | -5.4 | -1.5 | -2.3 | -1.8 | -2.7 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 3.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.4739 | 4.0448 | 3.2976 | 3.1795 | 3.4346 | 3.7261 | 1.6616 | 2.8924 | 2.5682 | 2.4123 | 2.2177 | 2.2739 | 5.9303 | 6.649 | 6.3603 | 6.2752 | 6.003 | 6.0208 |
Actin | 0.0477 | 0.0008 | 0.0088 | 0.0005 | 0.0008 | 0.0065 | 0.0134 | 0.0005 | 0.007 | 0.0062 | 0.0013 | 0.0007 | 0.0149 | 0.0017 | 0.0158 | 0.0007 | 0.0476 | 0.004 |
Bud | 0.0009 | 0.0006 | 0.0006 | 0.0005 | 0.0003 | 0.0002 | 0.0014 | 0.0011 | 0.0011 | 0.0153 | 0.0003 | 0.0055 | 0.0022 | 0.0001 | 0.0054 | 0.0004 | 0.001 | 0.003 |
Bud Neck | 0.0063 | 0.0003 | 0.0017 | 0.0005 | 0.0014 | 0.0012 | 0.0009 | 0.0003 | 0.0016 | 0.0015 | 0.0004 | 0.0058 | 0.0006 | 0.0002 | 0.0018 | 0.0004 | 0.0005 | 0.0009 |
Bud Periphery | 0.0019 | 0.0002 | 0.0007 | 0.0003 | 0.0003 | 0.0002 | 0.0019 | 0.0005 | 0.0005 | 0.0097 | 0.0003 | 0.0032 | 0.0041 | 0 | 0.0032 | 0.0002 | 0.0012 | 0.001 |
Bud Site | 0.0179 | 0.0033 | 0.0073 | 0.0008 | 0.0014 | 0.0001 | 0.0012 | 0.0069 | 0.0017 | 0.0027 | 0.0003 | 0.0007 | 0.0053 | 0.0007 | 0.0058 | 0.0005 | 0.0007 | 0.0008 |
Cell Periphery | 0.003 | 0.0006 | 0.0005 | 0.0004 | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0.0005 | 0.0001 | 0.0001 | 0.0008 | 0.0002 | 0.0007 | 0.0002 | 0.0001 | 0.0002 |
Cytoplasm | 0.5632 | 0.9473 | 0.8853 | 0.932 | 0.8476 | 0.9296 | 0.7328 | 0.9245 | 0.8714 | 0.8199 | 0.8403 | 0.8992 | 0.8525 | 0.974 | 0.8705 | 0.9208 | 0.7547 | 0.9223 |
Cytoplasmic Foci | 0.0215 | 0.0083 | 0.0169 | 0.0148 | 0.0356 | 0.0156 | 0.0303 | 0.0186 | 0.0218 | 0.0258 | 0.0223 | 0.0205 | 0.0171 | 0.0066 | 0.0121 | 0.0155 | 0.0305 | 0.0153 |
Eisosomes | 0.0012 | 0 | 0.0002 | 0 | 0 | 0 | 0.0002 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0004 | 0 | 0.001 | 0 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0248 | 0.0046 | 0.0065 | 0.0044 | 0.0012 | 0.0108 | 0.012 | 0.0033 | 0.0065 | 0.0066 | 0.0097 | 0.0052 | 0.0107 | 0.0021 | 0.0039 | 0.0093 | 0.0091 | 0.0115 |
Endosome | 0.0593 | 0.0094 | 0.0131 | 0.0136 | 0.044 | 0.0162 | 0.0814 | 0.024 | 0.0405 | 0.062 | 0.047 | 0.0371 | 0.0162 | 0.0043 | 0.009 | 0.0323 | 0.0521 | 0.0178 |
Golgi | 0.0121 | 0.0011 | 0.0042 | 0.0009 | 0.0134 | 0.0021 | 0.0103 | 0.0015 | 0.0059 | 0.0068 | 0.0052 | 0.0021 | 0.0034 | 0.0006 | 0.0021 | 0.0018 | 0.0032 | 0.0028 |
Lipid Particles | 0.0209 | 0.0002 | 0.0025 | 0.0004 | 0.0158 | 0.0002 | 0.0075 | 0.0004 | 0.0013 | 0.0024 | 0.006 | 0.0008 | 0.0016 | 0 | 0.0008 | 0.0003 | 0.0006 | 0.0002 |
Mitochondria | 0.0176 | 0.0005 | 0.0058 | 0.0002 | 0.0143 | 0.0015 | 0.0332 | 0.0009 | 0.0154 | 0.0023 | 0.042 | 0.0014 | 0.0035 | 0.0001 | 0.0006 | 0.0003 | 0.0017 | 0.001 |
None | 0.1161 | 0.017 | 0.0275 | 0.0209 | 0.0035 | 0.0107 | 0.0229 | 0.0094 | 0.0112 | 0.0075 | 0.0085 | 0.0092 | 0.052 | 0.0075 | 0.0626 | 0.0067 | 0.0881 | 0.0157 |
Nuclear Periphery | 0.0297 | 0.0007 | 0.0043 | 0.0012 | 0.0003 | 0.0006 | 0.0234 | 0.0009 | 0.0014 | 0.0026 | 0.0021 | 0.0023 | 0.0044 | 0.0003 | 0.0006 | 0.0021 | 0.0013 | 0.0006 |
Nucleolus | 0.0019 | 0.0001 | 0.0005 | 0.0001 | 0.0005 | 0 | 0.0004 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0003 | 0 | 0.0002 | 0 | 0.0001 | 0 |
Nucleus | 0.0089 | 0.0012 | 0.0021 | 0.0012 | 0.0006 | 0.001 | 0.006 | 0.0013 | 0.0017 | 0.0023 | 0.0015 | 0.0011 | 0.0041 | 0.0005 | 0.0008 | 0.0011 | 0.001 | 0.0007 |
Peroxisomes | 0.0141 | 0.0001 | 0.0027 | 0.0002 | 0.0106 | 0.0002 | 0.0023 | 0.0003 | 0.0009 | 0.0004 | 0.0006 | 0.0002 | 0.0013 | 0.0001 | 0.0006 | 0.0001 | 0.0015 | 0.0003 |
Punctate Nuclear | 0.0179 | 0.0003 | 0.0036 | 0.0009 | 0.0016 | 0.0001 | 0.002 | 0.0011 | 0.0005 | 0.0008 | 0.0009 | 0.0005 | 0.0013 | 0.0001 | 0.0005 | 0.0003 | 0.0017 | 0.0002 |
Vacuole | 0.0099 | 0.0033 | 0.0045 | 0.0057 | 0.0048 | 0.0024 | 0.0117 | 0.0035 | 0.0075 | 0.0202 | 0.0082 | 0.0033 | 0.0026 | 0.0007 | 0.0019 | 0.006 | 0.0023 | 0.0012 |
Vacuole Periphery | 0.0031 | 0.0002 | 0.0008 | 0.0007 | 0.0016 | 0.0006 | 0.0044 | 0.0007 | 0.0016 | 0.004 | 0.0029 | 0.001 | 0.0006 | 0.0001 | 0.0003 | 0.001 | 0.0007 | 0.0004 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 15.3251 | 15.3704 | 14.8767 | 20.1402 | 13.6906 | 16.4373 | 19.764 | 22.0043 | 20.6809 | 21.3526 |
Translational Efficiency | 0.4667 | 0.374 | 0.5299 | 0.3933 | 0.3914 | 0.49 | 0.4039 | 0.3023 | 0.3617 | 0.3532 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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