Standard name
Human Ortholog
Description Subunit of the CoA-Synthesizing Protein Complex (CoA-SPC); subunits of this complex are: Cab2p, Cab3p, Cab4p, Cab5p, Sis2p and Vhs3p; probable pantetheine-phosphate adenylyltransferase (PPAT); PPAT catalyzes the fourth step in the biosynthesis of coenzyme A from pantothenate; null mutant lethality is complemented by E. coli coaD (encoding PPAT) and by human COASY
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.97 0.98 0.99 0.99 0.98 0.95 0.88 – 0.69 0.74 0.74 0.74 0.99 1.0 0.99 0.97 0.96 0.97 0.96 0.94 0.95 0.96 0.96 0.94
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.06 0.08 – 0.4 0.28 0.29 0.28 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.06 – 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 1 0 1 0 0 0 0 0 0 0 1 0 1 0 1
Bud 0 1 0 0 1 0 0 – 1 2 8 7 0 0 0 0 0 0 1 0 0 3 2 3
Bud Neck 0 0 0 0 1 0 0 – 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 0 – 0 1 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 2 4 4 1 1 4 – 1 3 6 10 1 1 0 2 1 1 0 0 0 0 0 0
Cytoplasm 225 351 264 188 194 136 213 – 111 149 398 468 541 555 689 239 197 225 227 342 254 476 440 369
Endoplasmic Reticulum 0 4 1 0 1 2 2 – 0 2 3 6 1 0 0 11 6 5 0 1 1 1 3 0
Endosome 0 0 1 0 0 0 0 – 0 0 1 2 0 1 1 0 1 1 0 3 1 2 3 3
Golgi 2 1 1 0 0 0 0 – 0 0 0 0 1 1 0 1 0 1 2 1 1 0 0 0
Mitochondria 0 6 0 0 7 9 19 – 65 56 159 174 0 0 0 7 3 2 0 8 2 1 1 2
Nucleus 0 2 0 2 4 4 7 – 6 4 13 17 0 2 2 0 4 11 0 0 0 1 0 1
Nuclear Periphery 0 0 0 0 0 0 0 – 1 2 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 3
Vac/Vac Membrane 4 1 1 1 0 2 15 – 6 7 26 26 1 1 5 4 9 9 2 2 0 2 1 2
Unique Cell Count 231 359 267 189 198 143 241 162 200 540 630 544 557 693 246 206 232 237 364 267 496 460 395
Labelled Cell Count 232 368 272 195 209 154 260 191 227 614 713 545 561 697 264 221 255 237 364 267 496 460 395


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.6 5.3 6.9 6.4 5.4 5.2 5.7 – 4.7 5.2 5.0 5.2 7.0 6.9 6.4 6.4 8.0 7.3 7.4 7.4 7.5
Std Deviation (1e-4) 0.7 0.7 1.0 1.0 1.2 0.7 1.5 – 1.1 1.2 0.9 0.9 0.9 1.2 1.1 1.4 1.8 1.6 1.3 1.2 1.5
Intensity Change (Log2) – – – -0.11 -0.34 -0.41 -0.26 – -0.54 -0.41 -0.46 -0.41 0.03 -0.0 -0.1 -0.11 0.22 0.08 0.11 0.11 0.12


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.7 -0.8 -2.4 -4.9 – -9.2 -8.2 -8.8 -8.7 0.9 1.3 0.9 -1.4 -2.2 -1.5
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 3.1 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 4.7 – 0 9.2 9.9 9.6 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 2.7 0 0 0 0 0 3.6
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 3.8 – 0 0 3.3 3.0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.781 6.2482 5.8006 5.4413 5.4715 5.9423 6.786 6.4911 6.2431 5.9673 7.0581 6.5121 3.217 3.5847 3.4029 3.4245 3.3846 3.3166
Actin 0.0547 0.0062 0.0166 0.0733 0.0795 0.0054 0.041 0.0061 0.0133 0.0028 0.0345 0.0085 0.0395 0.0013 0.0126 0.0111 0.0614 0.0098
Bud 0.0012 0.0002 0.0032 0.0003 0.0008 0.002 0.0008 0.006 0.0026 0.0069 0.0051 0.0007 0.0012 0.0084 0.0016 0.0064 0.003 0.0061
Bud Neck 0.0149 0.0001 0.0006 0.0002 0.0004 0.0018 0.0052 0.0007 0.0035 0.0283 0.001 0.003 0.0163 0.0003 0.0103 0.0342 0.0115 0.0012
Bud Periphery 0.0018 0.0003 0.0009 0.0002 0.0016 0.0007 0.0011 0.0075 0.002 0.0111 0.0069 0.0012 0.0018 0.0018 0.0034 0.0066 0.004 0.003
Bud Site 0.005 0.0012 0.0026 0.0003 0.0006 0.0012 0.0054 0.0174 0.0112 0.0061 0.0097 0.0004 0.0081 0.0137 0.0154 0.0161 0.0186 0.0004
Cell Periphery 0.0003 0.0001 0.0002 0.0001 0.0001 0.0001 0.0006 0.0003 0.0003 0.0002 0.0002 0.0001 0.0004 0.0002 0.0005 0.0004 0.0004 0.0001
Cytoplasm 0.5944 0.7511 0.7062 0.615 0.5679 0.7806 0.5623 0.7631 0.6615 0.6288 0.6132 0.7457 0.4679 0.7328 0.6142 0.72 0.4785 0.7156
Cytoplasmic Foci 0.0203 0.0101 0.0136 0.0065 0.0144 0.0096 0.015 0.0051 0.0095 0.0254 0.0086 0.0086 0.0531 0.0056 0.0211 0.0072 0.0577 0.0135
Eisosomes 0.0004 0.0001 0.0001 0.0001 0.0003 0 0.0005 0.0001 0.0002 0 0.0001 0.0001 0.0003 0 0.0002 0.0004 0.0001 0
Endoplasmic Reticulum 0.0041 0.0017 0.0018 0.0019 0.0026 0.0018 0.0143 0.0022 0.0046 0.0025 0.0019 0.0032 0.0069 0.0017 0.0045 0.0025 0.0014 0.0014
Endosome 0.0198 0.0014 0.0054 0.0026 0.0145 0.0052 0.0223 0.0013 0.0082 0.0026 0.0048 0.0071 0.0332 0.0017 0.0093 0.0109 0.012 0.0048
Golgi 0.0062 0.0003 0.0029 0.0017 0.0093 0.0014 0.0047 0.0003 0.005 0.0006 0.0026 0.0025 0.0114 0.0003 0.0053 0.0022 0.0073 0.0025
Lipid Particles 0.0055 0.0003 0.0023 0.0002 0.0054 0.0017 0.0056 0.0001 0.002 0.0003 0.0008 0.0006 0.0143 0.0001 0.0028 0.0014 0.0043 0.0022
Mitochondria 0.0049 0.0002 0.0014 0.0004 0.0088 0.0041 0.0037 0.0003 0.0029 0.0003 0.0019 0.0021 0.0052 0.0004 0.0038 0.0231 0.0014 0.0013
None 0.2282 0.2214 0.2266 0.2932 0.2359 0.1664 0.2291 0.1794 0.2594 0.2727 0.2833 0.2042 0.2621 0.2248 0.2691 0.1324 0.283 0.2191
Nuclear Periphery 0.012 0.0006 0.001 0.0006 0.0023 0.001 0.0369 0.0015 0.003 0.0011 0.0027 0.0054 0.0175 0.0008 0.0036 0.0013 0.0011 0.001
Nucleolus 0.0002 0.0001 0.0008 0 0.0001 0.0002 0.001 0.0001 0.0002 0.0002 0.0006 0.0001 0.0008 0.0001 0.0011 0.0004 0.0011 0.0001
Nucleus 0.0018 0.0019 0.0033 0.0012 0.0014 0.0044 0.0334 0.0047 0.0042 0.0041 0.0122 0.0034 0.0095 0.0035 0.0049 0.0096 0.0033 0.0044
Peroxisomes 0.017 0.0003 0.006 0.001 0.0501 0.0081 0.0046 0.0004 0.0028 0.0018 0.0024 0.0004 0.0251 0.0002 0.0102 0.0031 0.0387 0.0102
Punctate Nuclear 0.0039 0.0018 0.0037 0.0006 0.0029 0.0032 0.0054 0.0024 0.0024 0.0028 0.0061 0.0015 0.0205 0.0013 0.0038 0.0008 0.0095 0.0022
Vacuole 0.0016 0.0006 0.0007 0.0004 0.0007 0.0007 0.0045 0.0008 0.0011 0.0011 0.0012 0.0009 0.0032 0.0009 0.0017 0.0088 0.0015 0.0007
Vacuole Periphery 0.0017 0.0001 0.0002 0.0001 0.0004 0.0004 0.0027 0.0001 0.0003 0.0001 0.0003 0.0004 0.0015 0.0001 0.0004 0.0011 0.0002 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 19.8324 11.8193 19.9328 26.1326 25.7136 19.2208 20.3702 20.1645 24.6875 25.561
Translational Efficiency 1.5628 1.863 1.1508 1.0805 1.1075 1.7689 1.1299 1.2248 1.1698 1.3331

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Subunit of the CoA-Synthesizing Protein Complex (CoA-SPC); subunits of this complex are: Cab2p, Cab3p, Cab4p, Cab5p, Sis2p and Vhs3p; probable pantetheine-phosphate adenylyltransferase (PPAT); PPAT catalyzes the fourth step in the biosynthesis of coenzyme A from pantothenate; null mutant lethality is complemented by E. coli coaD (encoding PPAT) and by human COASY
Localization
Cell Percentages cytoplasm (84%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Cab4

Cab4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cab4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available