Standard name
Human Ortholog
Description Cell wall protein with similarity to glucanases; scw4 scw10 double mutants exhibit defects in mating; SCW4 has a paralog, SCW10, that arose from the whole genome duplication
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.09 0 0.09 0.05 0.06 0.1 0.14 0.15 0.15 0.17 0.18 0.08 0.13 0.43 0.18 0.11 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.09 0.05 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.17 0.09 0.08 0 0.08 0.11 0.06 0 0.05 0.05 0.05 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.28 0.21 0.31 0.31 0.3 0.35 0.33 0.33 0.29 0.25 0.22 0.2 0.25 0.32 0.18 0.43 0.42 0.46 0.08 0.08 0 0.06 0.07 0.06
Nuclear Periphery 0.11 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0.1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.63 0.64 0.65 0.76 0.76 0.64 0.65 0.68 0.69 0.69 0.73 0.75 0.7 0.65 0.54 0.42 0.5 0.49 0.79 0.79 0.87 0.82 0.82 0.82
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0 0
Bud 1 0 0 0 0 0 2 0 2 1 2 0 0 0 0 0 0 0 0 2 3 4 3 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 1 0 0 0 3 0 0 0 0 0 0 0 0 0
Cytoplasm 4 14 12 27 14 23 61 92 65 71 79 100 23 50 163 30 27 18 0 1 1 2 0 4
Endoplasmic Reticulum 0 1 1 2 0 0 1 0 0 1 2 3 1 0 0 3 3 4 0 0 0 2 0 0
Endosome 1 0 8 4 7 0 1 1 0 0 1 0 20 34 21 3 5 0 4 3 5 6 10 12
Golgi 0 0 0 0 1 0 0 1 0 0 0 0 3 0 0 1 2 3 1 0 2 0 0 1
Mitochondria 38 13 24 1 23 44 38 19 23 25 22 34 8 5 2 7 10 7 5 2 4 4 5 2
Nucleus 61 31 89 91 91 137 210 222 129 117 102 109 77 123 68 74 100 97 18 12 10 17 22 19
Nuclear Periphery 23 3 12 3 7 5 6 8 2 1 2 2 5 2 1 1 0 1 7 3 6 1 5 7
Nucleolus 9 15 8 5 5 8 3 4 1 2 1 1 3 3 0 6 7 11 9 6 3 5 4 5
Peroxisomes 0 0 0 0 0 0 0 0 0 0 1 0 1 1 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 1 0 1 1 0 1 0 0 4 2 3 1 0 2 0 0 0 0 1 0
Vac/Vac Membrane 137 96 190 225 233 248 407 455 305 324 343 417 212 252 207 72 121 104 181 130 271 222 259 268
Unique Cell Count 219 149 291 295 305 386 630 672 439 468 473 556 305 386 382 171 240 213 230 165 313 270 317 327
Labelled Cell Count 274 173 344 359 382 466 730 804 527 543 557 666 357 472 468 198 275 247 230 165 313 270 317 327


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.3 15.1 11.8 13.4 15.3 13.9 15.1 17.2 16.9 16.5 16.4 15.9 9.9 7.6 6.4 14.2 14.2 15.2 13.2 12.3 13.1
Std Deviation (1e-4) 3.7 7.0 4.1 3.7 6.5 6.6 7.2 8.8 8.9 9.2 9.1 8.7 3.0 2.0 1.7 7.1 6.1 7.3 5.5 4.4 4.0
Intensity Change (Log2) – – – 0.18 0.37 0.24 0.36 0.55 0.52 0.48 0.47 0.43 -0.25 -0.63 -0.87 0.27 0.27 0.37 0.16 0.06 0.15


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2.4 0.3 1.1 2.9 4.4 4.6 4.7 5.2 5.7 1.8 3.9 11.3 4.8 3.1 2.0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome -1.2 -0.4 0 0 0 0 0 0 0 2.2 3.2 1.7 0 -0.5 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -4.7 -0.3 1.4 -1.2 -3.7 -1.6 -1.6 -2.0 -1.2 -3.0 -4.4 -5.2 -1.7 -1.9 -2.3
Nucleus 0.1 -0.2 1.3 0.8 0.7 -0.3 -1.7 -2.8 -3.6 -1.5 0.4 -3.9 2.8 2.7 3.4
Nuclear Periphery -2.4 -1.3 -2.3 -3.2 -2.9 -3.5 0 -3.7 0 -1.8 -3.3 -3.6 0 -3.2 -2.6
Nucleolus -0.9 -0.9 -0.6 0 0 0 0 0 0 -1.6 0 0 0.5 0.1 1.4
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 2.9 3.0 -0.3 -0.2 0.7 1.2 1.1 2.1 3.0 1.1 0 -2.9 -4.9 -3.5 -3.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.415 9.7354 8.1569 6.7717 8.3991 8.9013 4.9574 9.8048 8.3241 7.8968 8.3992 9.704 3.2455 8.1491 6.4839 4.808 6.1353 8.881
Actin 0.0049 0 0.0069 0 0 0.0004 0.0004 0.0003 0.0042 0 0.0397 0 0.0124 0.0019 0.0017 0.0819 0.035 0
Bud 0.0005 0.0002 0.0003 0.0038 0.0007 0.0004 0.0003 0.0002 0.0002 0.0001 0.0004 0.0002 0.0003 0.0002 0.0004 0.0011 0.0012 0.0002
Bud Neck 0.0014 0.0007 0.0007 0.0019 0.002 0.001 0.0007 0.0007 0.0005 0.0004 0.0011 0.0008 0.0005 0.0007 0.0007 0.0009 0.0019 0.0008
Bud Periphery 0.0015 0.0008 0.0011 0.0148 0.0026 0.0017 0.001 0.0006 0.0009 0.0005 0.0007 0.0009 0.0008 0.0007 0.0011 0.0024 0.0015 0.0009
Bud Site 0.0014 0.0003 0.0005 0.0034 0.0014 0.0006 0.0013 0.0014 0.0004 0.0002 0.001 0.0003 0.0009 0.0004 0.0005 0.0019 0.0203 0.0004
Cell Periphery 0.0074 0.0096 0.0081 0.0068 0.0117 0.0155 0.0067 0.0108 0.0093 0.0073 0.0046 0.0102 0.0047 0.0098 0.0059 0.0048 0.008 0.0093
Cytoplasm 0.0016 0.0004 0.0003 0.0005 0.0004 0.0004 0.0009 0.0008 0.0003 0.0002 0.002 0.0003 0.0004 0.0003 0.0006 0.0012 0.0008 0.0003
Cytoplasmic Foci 0.0039 0.0007 0.0021 0.0022 0.0011 0.0007 0.0135 0.0006 0.0021 0.0015 0.0235 0.0026 0.0218 0.0017 0.0032 0.012 0.0122 0.0004
Eisosomes 0.0002 0 0.0001 0 0 0 0 0 0 0 0.0001 0 0.0003 0 0 0.0008 0.0002 0
Endoplasmic Reticulum 0.0029 0.0012 0.001 0.0004 0.0006 0.0008 0.0013 0.0006 0.0007 0.0002 0.0033 0.0006 0.0015 0.0008 0.0015 0.0065 0.0009 0.0006
Endosome 0.0616 0.0292 0.0278 0.0603 0.0488 0.0287 0.0513 0.0167 0.0376 0.0326 0.0581 0.018 0.0542 0.0316 0.0377 0.0943 0.0683 0.014
Golgi 0.0052 0.0013 0.0037 0.0027 0.0077 0.0039 0.0062 0.0008 0.0048 0.0032 0.0115 0.0007 0.0093 0.0035 0.0054 0.067 0.0088 0.0009
Lipid Particles 0.0248 0.0045 0.023 0.0177 0.0182 0.0194 0.0448 0.0054 0.0188 0.0207 0.0413 0.0087 0.0662 0.0145 0.0246 0.0944 0.0866 0.0153
Mitochondria 0.031 0.0071 0.0783 0.0033 0.0149 0.0289 0.0418 0.0016 0.027 0.0057 0.008 0.0028 0.0598 0.0214 0.0653 0.0147 0.0296 0.0025
None 0.0023 0 0.0002 0 0.0001 0.0001 0.0008 0 0.0001 0 0.0012 0.0001 0.0005 0.0001 0.0002 0.004 0.0007 0
Nuclear Periphery 0.0186 0.0031 0.002 0.0009 0.0015 0.0019 0.0038 0.0025 0.0056 0.0005 0.0208 0.0012 0.004 0.0053 0.0051 0.0825 0.0019 0.0015
Nucleolus 0.0124 0.0044 0.011 0.0049 0.0109 0.0106 0.0222 0.0099 0.0167 0.0042 0.0071 0.0071 0.0065 0.0086 0.0102 0.004 0.0159 0.0132
Nucleus 0.01 0.0024 0.0027 0.0049 0.0041 0.0051 0.0072 0.0033 0.0054 0.0017 0.0067 0.0066 0.0021 0.0042 0.0068 0.0039 0.0035 0.0042
Peroxisomes 0.0032 0.0003 0.0024 0.001 0.0011 0.0006 0.0037 0.0002 0.0018 0.0022 0.0054 0.0004 0.0161 0.0048 0.0026 0.0115 0.0085 0.0003
Punctate Nuclear 0.0019 0 0.0001 0 0 0 0.0022 0.0001 0.0001 0 0.0043 0.0032 0.001 0 0.0003 0.0231 0.0009 0
Vacuole 0.4476 0.5596 0.4856 0.5643 0.6145 0.5648 0.4374 0.5947 0.5008 0.6711 0.5176 0.6026 0.3702 0.4815 0.412 0.142 0.4574 0.5811
Vacuole Periphery 0.3556 0.374 0.3421 0.306 0.2576 0.3142 0.3523 0.3489 0.3626 0.2477 0.2418 0.3326 0.3665 0.4078 0.414 0.3451 0.2359 0.3541

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 488.5012 506.3568 796.8684 480.8416 300.0466 547.1224 636.7497 653.9936 585.9135 343.1831
Translational Efficiency 1.8945 2.2467 1.8183 2.0015 2.1983 1.9215 2.0074 2.31 1.7563 1.8512

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Cell wall protein with similarity to glucanases; scw4 scw10 double mutants exhibit defects in mating; SCW4 has a paralog, SCW10, that arose from the whole genome duplication
Localization
Cell Percentages vacuole (57%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Scw4

Scw4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Scw4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available