Standard name | |
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Human Ortholog | |
Description | Biotin synthase; catalyzes the conversion of dethiobiotin to biotin, which is the last step of the biotin biosynthesis pathway; complements E. coli bioB mutant |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 8 | 6 | 6 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 3 | 1 | 1 | 0 | 1 | 1 | 3 | 5 | 7 | 2 | 4 | 11 | 14 |
Bud | 3 | 4 | 14 | 9 | 10 | 13 | 34 | 28 | 12 | 12 | 16 | 14 | 0 | 2 | 0 | 1 | 3 | 8 | 2 | 1 | 0 | 2 | 4 | 7 |
Bud Neck | 1 | 0 | 3 | 0 | 4 | 1 | 2 | 7 | 1 | 8 | 1 | 1 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 2 | 4 |
Bud Site | 0 | 2 | 3 | 1 | 0 | 0 | 1 | 8 | 9 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | – | – | – | – | – | – |
Cell Periphery | 0 | 19 | 6 | 10 | 4 | 3 | 5 | 3 | 2 | 0 | 1 | 3 | 2 | 1 | 1 | 8 | 4 | 12 | 0 | 0 | 1 | 0 | 1 | 1 |
Cytoplasm | 23 | 7 | 20 | 32 | 44 | 59 | 74 | 98 | 91 | 114 | 71 | 54 | 113 | 168 | 162 | 1 | 3 | 10 | 5 | 0 | 4 | 20 | 34 | 50 |
Endoplasmic Reticulum | 3 | 9 | 4 | 3 | 5 | 2 | 0 | 1 | 1 | 2 | 0 | 0 | 3 | 1 | 0 | 2 | 0 | 5 | 7 | 1 | 1 | 1 | 3 | 1 |
Endosome | 0 | 27 | 14 | 7 | 28 | 4 | 4 | 4 | 0 | 1 | 0 | 0 | 2 | 8 | 2 | 8 | 5 | 9 | 13 | 14 | 17 | 10 | 16 | 18 |
Golgi | 1 | 12 | 2 | 2 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 17 | 1 | 14 | 6 | 18 | 4 | 2 | 1 | 2 |
Mitochondria | 108 | 208 | 180 | 153 | 124 | 235 | 273 | 249 | 217 | 210 | 178 | 143 | 8 | 2 | 1 | 10 | 1 | 6 | 118 | 215 | 258 | 116 | 145 | 145 |
Nucleus | 0 | 1 | 5 | 0 | 5 | 2 | 6 | 5 | 5 | 3 | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 6 | 0 | 0 | 0 | 0 | 1 | 0 |
Nuclear Periphery | 0 | 9 | 0 | 0 | 0 | 0 | 2 | 1 | 3 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 1 | 1 | 3 | 3 | 1 | 0 |
Nucleolus | 2 | 4 | 0 | 0 | 1 | 2 | 1 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 0 | 1 | 1 | 1 | 1 | 1 |
Peroxisomes | 1 | 22 | 22 | 22 | 20 | 3 | 3 | 7 | 2 | 3 | 1 | 0 | 1 | 0 | 5 | 7 | 6 | 19 | 4 | 11 | 12 | 5 | 4 | 2 |
SpindlePole | 2 | 6 | 34 | 20 | 29 | 20 | 24 | 40 | 17 | 23 | 2 | 14 | 0 | 2 | 0 | 7 | 3 | 7 | 4 | 1 | 2 | 5 | 8 | 8 |
Vac/Vac Membrane | 5 | 33 | 16 | 0 | 18 | 12 | 9 | 10 | 8 | 6 | 8 | 8 | 0 | 5 | 2 | 12 | 3 | 23 | 7 | 12 | 3 | 11 | 9 | 11 |
Unique Cell Count | 131 | 257 | 243 | 207 | 232 | 291 | 342 | 365 | 288 | 309 | 229 | 188 | 131 | 184 | 171 | 60 | 24 | 105 | 186 | 291 | 321 | 191 | 257 | 278 |
Labelled Cell Count | 150 | 371 | 329 | 265 | 295 | 356 | 439 | 464 | 369 | 384 | 282 | 240 | 135 | 191 | 174 | 77 | 32 | 130 | 186 | 291 | 321 | 191 | 257 | 278 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.7 | 6.6 | 5.3 | 4.9 | 5.2 | 4.0 | 3.7 | 4.2 | 3.5 | 3.5 | 3.2 | 3.5 | 4.9 | 5.3 | 5.3 | 7.9 | 7.2 | 7.3 | 5.1 | 5.2 | 5.4 |
Std Deviation (1e-4) | 0.7 | 3.2 | 1.2 | 1.6 | 1.8 | 1.2 | 1.5 | 1.9 | 1.5 | 1.8 | 1.1 | 1.0 | 1.4 | 1.4 | 1.9 | 2.2 | 2.1 | 2.3 | 1.9 | 2.3 | 2.7 |
Intensity Change (Log2) | – | – | – | -0.09 | -0.02 | -0.4 | -0.53 | -0.32 | -0.59 | -0.58 | -0.7 | -0.58 | -0.1 | 0.01 | 0.02 | 0.58 | 0.46 | 0.46 | -0.05 | -0.01 | 0.03 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | -0.7 | -0.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -2.5 | -3.2 | 0 | 0 | 0.7 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 1.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.5 |
Cytoplasm | 2.4 | 3.4 | 3.9 | 4.4 | 5.7 | 6.6 | 7.8 | 6.3 | 5.6 | 15.0 | 17.1 | 17.5 | 0 | 0 | 0.4 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | -1.2 | 2.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -1.9 | -0.7 | -2.4 | 0 | 0 | 1.0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | -4.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -12.5 | -15.1 | -14.8 | -8.2 | -7.0 | -11.7 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0.6 | -0.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -3.2 | -4.2 | -2.5 | 0.6 | 0 | 2.4 |
SpindlePole | -1.4 | -0.5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -4.5 | -4.8 | -5.1 | -0.5 | 0 | -1.9 |
Vacuole | -3.8 | 0.5 | -1.3 | -2.3 | -2.3 | -2.1 | -2.8 | -1.5 | -1.0 | -3.0 | -1.8 | -2.7 | 3.2 | 0 | 4.2 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 5.8971 | 6.7119 | 5.7962 | 5.2639 | 5.3491 | 5.8873 | 4.7415 | 4.0984 | 4.9708 | 3.825 | 3.4703 | 4.2162 | 6.2311 | 6.6082 | 6.024 | 5.661 | 5.4292 | 5.4129 |
Actin | 0.0268 | 0.0067 | 0.0097 | 0.0103 | 0.0178 | 0.0098 | 0.0206 | 0.0069 | 0.0103 | 0.0013 | 0.0015 | 0.0026 | 0.0502 | 0.0093 | 0.0318 | 0.0047 | 0.0018 | 0.0105 |
Bud | 0.0004 | 0.0007 | 0.0001 | 0.0024 | 0.0003 | 0.0003 | 0.0013 | 0.0021 | 0.0015 | 0.0001 | 0.0003 | 0.0002 | 0.0009 | 0.0009 | 0.0004 | 0.0012 | 0.0001 | 0.0001 |
Bud Neck | 0.0021 | 0.0003 | 0.0001 | 0.0007 | 0.0004 | 0.0002 | 0.0014 | 0.0006 | 0.0005 | 0.0002 | 0.0003 | 0.0003 | 0.0008 | 0.0004 | 0.0022 | 0.0005 | 0.0002 | 0.0002 |
Bud Periphery | 0.0007 | 0.0009 | 0.0003 | 0.0097 | 0.0006 | 0.0005 | 0.0032 | 0.0034 | 0.0021 | 0.0004 | 0.0009 | 0.0009 | 0.0013 | 0.0014 | 0.0006 | 0.0048 | 0.0003 | 0.0004 |
Bud Site | 0.0058 | 0.0089 | 0.0006 | 0.0018 | 0.001 | 0.0002 | 0.0045 | 0.0146 | 0.0142 | 0.0004 | 0.0002 | 0.0003 | 0.0029 | 0.0039 | 0.0013 | 0.0018 | 0.0003 | 0.0002 |
Cell Periphery | 0.0005 | 0.0002 | 0.0004 | 0.0003 | 0.0001 | 0.0001 | 0.0007 | 0.0008 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0.0003 | 0.0001 | 0.002 | 0.0001 | 0.0001 |
Cytoplasm | 0.0008 | 0.0005 | 0.0003 | 0.0018 | 0.0003 | 0.0001 | 0.0029 | 0.0014 | 0.0008 | 0.0001 | 0.0016 | 0.0011 | 0.0003 | 0.0006 | 0.0005 | 0.0002 | 0.0001 | 0.0001 |
Cytoplasmic Foci | 0.0151 | 0.0098 | 0.003 | 0.0052 | 0.0101 | 0.0083 | 0.0192 | 0.0142 | 0.0295 | 0.013 | 0.0218 | 0.0177 | 0.0062 | 0.0063 | 0.0124 | 0.0043 | 0.0051 | 0.0024 |
Eisosomes | 0.0014 | 0.0002 | 0.0019 | 0.0002 | 0.0003 | 0.0005 | 0.0004 | 0.0003 | 0.0003 | 0.0001 | 0.0001 | 0.0002 | 0.0006 | 0.0002 | 0.0003 | 0.0003 | 0.0001 | 0.0002 |
Endoplasmic Reticulum | 0.0014 | 0.0001 | 0.0006 | 0.0007 | 0.0002 | 0.0001 | 0.0005 | 0.0004 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0005 | 0.0003 | 0.0004 | 0.0001 | 0.0002 | 0.0002 |
Endosome | 0.0232 | 0.023 | 0.0063 | 0.0173 | 0.0062 | 0.0072 | 0.0307 | 0.0215 | 0.019 | 0.0109 | 0.0108 | 0.0161 | 0.0181 | 0.021 | 0.019 | 0.0073 | 0.0278 | 0.0051 |
Golgi | 0.0404 | 0.0376 | 0.0127 | 0.0173 | 0.0172 | 0.0277 | 0.0227 | 0.0407 | 0.0182 | 0.0171 | 0.02 | 0.0278 | 0.0346 | 0.0428 | 0.0344 | 0.0104 | 0.0305 | 0.0219 |
Lipid Particles | 0.0316 | 0.0035 | 0.0103 | 0.0108 | 0.0057 | 0.0108 | 0.0258 | 0.0104 | 0.0068 | 0.0141 | 0.0048 | 0.0139 | 0.0319 | 0.0057 | 0.0092 | 0.0077 | 0.0173 | 0.0085 |
Mitochondria | 0.8023 | 0.8789 | 0.9301 | 0.8883 | 0.8687 | 0.9109 | 0.7918 | 0.8278 | 0.8267 | 0.8912 | 0.901 | 0.8683 | 0.8113 | 0.881 | 0.8686 | 0.9265 | 0.8713 | 0.9263 |
None | 0.0027 | 0.0001 | 0.0002 | 0.0006 | 0.0007 | 0.0001 | 0.0101 | 0.0013 | 0.0009 | 0.0001 | 0.0217 | 0.0071 | 0.0005 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0001 |
Nuclear Periphery | 0.0021 | 0.0001 | 0.0001 | 0.0014 | 0.0018 | 0 | 0.0014 | 0.0013 | 0.0002 | 0 | 0.0002 | 0 | 0.0063 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0002 |
Nucleolus | 0.0011 | 0.0001 | 0 | 0.0003 | 0.0014 | 0 | 0.001 | 0.0013 | 0.0007 | 0.0001 | 0.0001 | 0.0001 | 0.0009 | 0.0003 | 0.0001 | 0.0009 | 0.0002 | 0.0001 |
Nucleus | 0.0005 | 0.0001 | 0.0001 | 0.0005 | 0.001 | 0 | 0.0006 | 0.0004 | 0.0006 | 0 | 0.0001 | 0.0001 | 0.0009 | 0.0003 | 0.0001 | 0.0003 | 0.0001 | 0.0001 |
Peroxisomes | 0.0355 | 0.0231 | 0.0215 | 0.0241 | 0.0444 | 0.0194 | 0.0503 | 0.0446 | 0.061 | 0.0495 | 0.0067 | 0.0407 | 0.019 | 0.0191 | 0.015 | 0.018 | 0.0393 | 0.0198 |
Punctate Nuclear | 0.0022 | 0.0001 | 0.0001 | 0.0008 | 0.0201 | 0.002 | 0.001 | 0.0002 | 0.0017 | 0.0001 | 0.001 | 0.0005 | 0.0005 | 0.0001 | 0.0006 | 0 | 0.0001 | 0 |
Vacuole | 0.0013 | 0.0018 | 0.0006 | 0.0034 | 0.0004 | 0.0005 | 0.0067 | 0.0037 | 0.0024 | 0.0004 | 0.0023 | 0.0007 | 0.003 | 0.0026 | 0.0012 | 0.0048 | 0.0018 | 0.0006 |
Vacuole Periphery | 0.0018 | 0.0035 | 0.0009 | 0.0022 | 0.0012 | 0.0012 | 0.003 | 0.0021 | 0.0019 | 0.0006 | 0.0045 | 0.0011 | 0.0089 | 0.0032 | 0.0018 | 0.004 | 0.003 | 0.0029 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 126.6763 | 35.8523 | 21.413 | 21.2675 | 30.4386 | 82.1229 | 22.1038 | 13.4267 | 15.419 | 17.2659 |
Translational Efficiency | 1.2382 | 1.2822 | 1.0077 | 1.2138 | 1.2752 | 1.6606 | 1.0403 | 1.4786 | 1.0375 | 1.1713 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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