Standard name
Human Ortholog
Description Ceramide synthase component; involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lac1p; forms ER foci upon DNA replication stress; homolog of human CERS2, a tumor metastasis suppressor gene whose silencing enhances invasion/metastasis of prostate cancer cells; LAG1 has a paralog, LAC1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.09 0.06 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 – 0.07 0 0 0 0 0 0.05 0.1 0.31 0.13 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0.7 0.72 0.23 0.05 0 0 – 0 0 0 0.05 0 0.43 0.82 0.24 0 0 0.07 0.88 0.73 0.84 0.6 0.69 0.63
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0.05 0 0.1 0.05 0 0 – 0 0 0 0 0 0 0.07 0.06 0 0.1 0.17 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 – 0 0 0 0.05 0 0 0 0 0.12 0.11 0.12 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0.05 – 0 0 0 0.07 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Nuclear Periphery 0.53 0.21 0.25 0.37 0.27 0.46 – 0.43 0.56 0.42 0.44 0.41 0.5 0.25 0.15 0.25 0.23 0.26 0.09 0.18 0.1 0.3 0.21 0.18
Nucleolus 0 0 0 0 0.1 0.05 – 0.08 0.11 0.05 0.08 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.16 0.21 0.47 0.49 0.57 0.49 – 0.45 0.43 0.53 0.42 0.51 0.25 0.07 0.44 0.38 0.53 0.35 0 0 0 0.05 0.06 0.1
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 4 9 8 1 2 – 7 1 3 4 4 2 7 14 0 2 0 0 2 7 2 4 8
Bud 0 1 0 1 0 2 – 1 1 4 4 3 0 0 1 0 0 0 0 0 3 2 5 12
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 1 0 1 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 8 0 0 0 – 3 1 0 2 3 4 14 29 1 0 3 0 0 0 0 0 0
Cytoplasm 0 4 15 10 17 10 – 54 11 8 15 20 7 23 35 5 16 6 0 0 2 0 0 4
Endoplasmic Reticulum 45 65 79 15 3 7 – 32 17 18 20 25 123 374 79 0 3 7 76 81 457 306 410 530
Endosome 0 0 3 4 10 6 – 3 4 9 8 8 0 0 1 0 1 0 0 0 1 4 4 15
Golgi 3 0 36 14 6 1 – 23 6 7 1 12 12 30 19 0 12 16 0 0 1 3 5 6
Mitochondria 0 1 9 12 7 7 – 13 10 23 19 18 0 0 0 2 13 12 0 0 3 4 5 11
Nucleus 1 0 2 3 3 20 – 25 9 23 29 29 3 2 4 1 3 1 0 0 0 0 1 1
Nuclear Periphery 34 19 87 113 107 192 – 338 261 249 187 282 142 114 49 4 27 25 7 20 51 155 123 154
Nucleolus 1 0 3 9 41 21 – 64 50 31 32 61 1 0 6 0 3 4 0 0 0 2 1 1
Peroxisomes 0 0 0 0 4 1 – 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
SpindlePole 0 0 2 0 13 0 – 0 0 0 0 0 0 0 5 0 0 0 0 0 2 1 0 7
Vac/Vac Membrane 10 19 163 149 228 205 – 355 203 316 178 349 72 34 149 6 63 34 0 3 6 25 34 86
Unique Cell Count 64 90 349 303 400 420 787 468 598 421 681 283 454 336 16 119 96 87 111 543 511 598 845
Labelled Cell Count 94 113 416 338 440 474 918 575 691 500 814 366 598 391 19 143 108 87 111 543 511 598 845


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 17.8 14.1 21.1 21.2 20.2 16.5 – 17.2 15.9 16.0 15.8 16.4 17.5 18.6 18.5 12.8 18.8 22.7 20.6 21.0 21.8
Std Deviation (1e-4) 2.9 2.7 4.3 4.4 4.5 3.5 – 5.2 3.9 3.7 4.4 4.1 2.9 3.2 3.6 3.2 4.7 5.6 3.2 3.7 4.0
Intensity Change (Log2) – – – 0.01 -0.06 -0.35 – -0.29 -0.41 -0.4 -0.41 -0.36 -0.26 -0.18 -0.19 -0.72 -0.16 0.11 -0.03 -0.01 0.05


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 -1.7 0 0 -1.0 1.2 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 -0.8 0.7 3.7 0 0 0
Cytoplasm -0.7 0 -1.5 – 1.7 -1.6 -2.9 -0.5 -1.1 -1.2 0.5 3.1 0 3.5 0
Endoplasmic Reticulum -6.4 -9.6 -9.2 – -9.7 -8.3 -9.6 -7.4 -9.6 5.6 16.9 0.3 0 -5.0 -3.4
Endosome 0 1.7 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi -2.7 -5.2 -6.5 – -5.2 -5.8 -6.5 -6.5 -6.2 -2.9 -1.9 -2.2 0 -0.1 1.7
Mitochondria 1.0 -0.8 -0.9 – -1.0 -0.4 1.0 1.4 0.1 0 0 0 0 3.7 0
Nucleus 0 0 3.5 – 2.7 0 3.0 4.4 3.3 0 0 0 0 0 0
Nuclear Periphery 3.4 0.6 6.0 – 5.8 8.8 5.2 5.6 5.2 6.6 0.1 -3.4 0 -0.5 0.2
Nucleolus 2.0 5.5 3.3 – 4.8 5.6 3.5 4.5 5.1 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 2.6 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0.6 2.8 0.6 – -0.5 -0.9 1.8 -1.2 1.4 -5.5 -12.8 -0.6 -0.7 1.2 -2.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0095 0.0002 0.0041 0.0005 0.0119 0.0006 0.0279 0.0004 0.0154 0.0006 0.0188 0.0004 0.0145 0.0017 0.0027 0.0002 0.0004 0.0008
Bud 0.0001 0 0.0001 0 0.0003 0 0.0001 0 0.0001 0 0.0002 0 0.0002 0.0001 0.0004 0 0.0002 0
Bud Neck 0.0007 0.0004 0.0005 0.0002 0.0005 0.0004 0.0002 0.0004 0.0002 0.0001 0.0006 0.0001 0.0002 0.0002 0.0009 0.0001 0.0012 0.0001
Bud Periphery 0.0002 0.0001 0.0003 0.0001 0.0007 0.0001 0.0002 0.0003 0.0002 0.0001 0.0003 0 0.0002 0.0007 0.0003 0.0001 0.0002 0.0001
Bud Site 0.0004 0.0001 0.0008 0 0.0006 0.0002 0.0006 0.0003 0.0013 0 0.001 0 0.0003 0.0001 0.0064 0 0.0041 0
Cell Periphery 0.0004 0.0026 0.0012 0.0007 0.0002 0.0004 0.0004 0.002 0.0011 0.0003 0.0002 0.0001 0.0001 0.0019 0.0003 0.0008 0.0003 0.0002
Cytoplasm 0.004 0.0039 0.0058 0.0014 0.0005 0.001 0.0203 0.0004 0.001 0.0008 0.0007 0.0002 0.0167 0.0003 0.0003 0.0005 0.0002 0
Cytoplasmic Foci 0.0049 0.0224 0.0173 0.0059 0.0041 0.006 0.0122 0.0778 0.0265 0.015 0.0116 0.0036 0.0003 0.0001 0.1909 0 0.0781 0.0001
Eisosomes 0.0005 0.0001 0.0002 0 0.0001 0 0.0002 0.0002 0.0001 0 0.0001 0 0.0001 0.0001 0.0008 0 0.0002 0.0002
Endoplasmic Reticulum 0.1102 0.3321 0.2091 0.4244 0.3367 0.2091 0.1057 0.333 0.2831 0.4548 0.425 0.1821 0.0172 0.1325 0.0001 0.944 0.0001 0.0073
Endosome 0.0034 0.0006 0.0076 0.0011 0.0111 0.0026 0.0262 0.0009 0.002 0.001 0.0093 0.0005 0.0103 0.0024 0.0008 0.0016 0.0009 0.0004
Golgi 0.0025 0.0003 0.0028 0.0003 0.009 0.0009 0.0065 0.0015 0.0017 0.0006 0.0074 0.0008 0.0003 0.0014 0.0037 0.0002 0.0023 0.001
Lipid Particles 0.006 0.0098 0.0147 0.0082 0.0147 0.0143 0.032 0.0644 0.0595 0.0477 0.067 0.0155 0.0001 0.0025 0.099 0.0003 0.0658 0.0045
Mitochondria 0.0013 0.0005 0.0023 0.0004 0.0039 0.0014 0.0019 0.0006 0.0004 0.0007 0.0029 0.0001 0.0098 0.003 0.0111 0.0001 0.0011 0.0008
None 0.0052 0.0001 0.0014 0.0001 0.0004 0.0008 0.0093 0.0003 0.0002 0.0001 0.0005 0.0001 0.004 0.0003 0.0014 0 0.0005 0.0001
Nuclear Periphery 0.7694 0.4806 0.517 0.4826 0.5348 0.6825 0.6859 0.3548 0.4364 0.4003 0.3225 0.7421 0.6467 0.7304 0.0011 0.0445 0.0009 0.9502
Nucleolus 0.0092 0.0213 0.0534 0.0036 0.0025 0.0062 0.0015 0.0422 0.0432 0.0028 0.0301 0.0027 0.0008 0.0001 0.153 0 0.463 0.0015
Nucleus 0.0199 0.0421 0.0381 0.0109 0.0468 0.0162 0.0142 0.008 0.0122 0.0276 0.0301 0.0047 0.2633 0.0052 0.0125 0.0009 0.0293 0.0037
Peroxisomes 0.0003 0 0.0066 0 0.0041 0.0001 0.0005 0.0009 0.0003 0.0001 0.0028 0 0.0001 0 0.0149 0 0.0029 0
Punctate Nuclear 0.0235 0.0582 0.093 0.0377 0.0028 0.0404 0.019 0.0813 0.0805 0.0174 0.0554 0.0257 0.0046 0.0003 0.4983 0 0.3476 0.0016
Vacuole 0.0048 0.0107 0.0051 0.0061 0.0038 0.0046 0.0038 0.0076 0.0053 0.0032 0.0023 0.0014 0.002 0.009 0.0003 0.0045 0.0004 0.0004
Vacuole Periphery 0.0235 0.0139 0.0187 0.0156 0.0105 0.0118 0.0315 0.0225 0.0294 0.0266 0.0112 0.0199 0.0081 0.1078 0.0006 0.0021 0.0002 0.027

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 70.525 64.6412 53.2961 57.5458 59.0301 64.2404 73.6293 60.1784 50.4592 74.135
Translational Efficiency 1.161 1.148 0.9316 1.0434 1.0967 1.248 0.9274 1.0164 1.1284 0.8944

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✔
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Ceramide synthase component; involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lac1p; forms ER foci upon DNA replication stress; homolog of human CERS2, a tumor metastasis suppressor gene whose silencing enhances invasion/metastasis of prostate cancer cells; LAG1 has a paralog, LAC1, that arose from the whole genome duplication
Localization
Cell Percentages ER (64%)
Cell Cycle Regulation No
Subcompartmental Group ER-5

Lag1

Lag1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Lag1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available