Standard name
Human Ortholog
Description Cdc42p-activated signal transducing kinase; involved in pheromone response, pseudohyphal/invasive growth, vacuole inheritance, down-regulation of sterol uptake; GBB motif binds Ste4p; member of the PAK (p21-activated kinase) family
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.22 0.1 0.23 0.18 0.14 0.09 0.09 0.08 0.07 0.07 0.05 0.05 0.17 0.24 0.15 0.09 0 0.06 0.16 0.12 0.1 0.15 0.22 0.18
Bud 0.09 0 0.15 0.14 0.13 0.08 0.06 0.07 0.1 0.09 0.08 0.07 0.11 0.12 0.13 0.09 0 0.05 0.15 0.16 0.13 0.44 0.36 0.33
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.21 0.13 0.14 0.14 0.07
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.15 0.05 0.16 0.13 0.09 0.07 0.09 0.08 0.12 0.13 0.13 0.14 0.34 0.36 0.39 0.3 0.37 0.33 0.11 0.05 0 0 0 0
Cytoplasm 0.54 0.81 0.63 0.67 0.71 0.73 0.71 0.65 0.61 0.64 0.58 0.61 0.53 0.58 0.67 0.69 0.75 0.73 0.43 0.4 0.5 0.19 0.21 0.3
Endoplasmic Reticulum 0.13 0.1 0.07 0.06 0.08 0.06 0.08 0.06 0 0 0.07 0.05 0.13 0.07 0 0.05 0 0 0 0 0.05 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.05 0 0.06 0 0.17 0.15 0.17 0.33 0.31 0.32 0.34 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.05 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 35 2 53 65 74 48 63 55 29 33 21 25 23 47 35 19 1 11 28 7 27 16 46 51
Bud 14 0 34 51 66 42 43 48 41 46 33 35 15 24 29 19 4 9 26 9 37 50 76 93
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 15 12 34 15 28 19
Bud Site 2 0 5 6 3 9 6 7 4 8 4 6 1 0 0 0 0 0 – – – – – –
Cell Periphery 24 1 36 47 45 36 64 54 49 62 52 67 46 71 90 68 35 60 19 2 12 1 0 4
Cytoplasm 84 17 143 238 372 382 499 452 251 316 237 300 71 115 152 153 71 132 75 24 138 21 44 84
Endoplasmic Reticulum 20 2 15 23 41 31 56 39 15 16 28 26 17 14 5 12 2 6 5 0 12 1 4 12
Endosome 0 0 1 3 6 0 5 3 1 1 0 0 0 0 0 2 0 0 0 0 1 2 2 2
Golgi 3 0 1 0 0 0 0 0 0 0 0 0 0 0 0 5 0 3 1 0 0 0 0 0
Mitochondria 1 1 1 23 20 89 103 119 137 152 132 167 1 0 5 7 0 3 1 0 6 0 1 2
Nucleus 0 0 0 1 0 1 2 7 1 4 2 1 0 0 1 11 2 1 0 1 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 1 1 0 0 1 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 1 0 0 1 2 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 2 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1
SpindlePole 1 0 0 1 2 0 0 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0
Vac/Vac Membrane 3 1 0 5 7 2 15 45 9 10 15 21 0 0 1 5 2 9 0 0 5 0 1 3
Unique Cell Count 156 21 228 356 524 526 703 697 414 492 407 493 134 198 228 223 95 181 176 61 279 114 213 282
Labelled Cell Count 187 24 289 464 638 640 857 832 538 651 526 648 174 273 318 301 117 234 176 61 279 114 213 282


Bud, Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 13.5 9.5 8.0 7.8 7.6 6.6 6.3 6.3 5.5 5.5 5.5 5.3 11.8 11.5 9.7 12.3 14.0 14.7 10.8 11.5 12.6
Std Deviation (1e-4) 1.9 1.1 1.1 1.1 1.0 1.2 1.2 1.0 0.9 0.8 1.0 1.0 1.8 2.0 2.0 2.4 2.8 2.7 2.1 2.1 2.3
Intensity Change (Log2) – – – -0.03 -0.07 -0.27 -0.35 -0.34 -0.54 -0.54 -0.54 -0.59 0.57 0.53 0.29 0.63 0.81 0.88 0.44 0.54 0.66


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -1.5 -3.1 -5.2 -5.7 -6.3 -5.9 -6.4 -6.8 -7.3 -1.4 0.1 -2.1 -4.3 -4.9 -4.7
Bud -0.2 -0.9 -2.9 -4.2 -3.7 -1.9 -2.2 -2.7 -3.3 -1.0 -0.8 -0.7 -2.1 -2.7 -3.3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -0.9 -2.9 -3.8 -2.8 -3.6 -1.4 -1.2 -1.1 -0.8 4.1 4.8 5.7 3.7 4.2 4.1
Cytoplasm 1.0 2.2 2.7 2.3 0.6 -0.5 0.4 -1.1 -0.5 -1.8 -1.0 0.9 1.3 2.1 2.2
Endoplasmic Reticulum -0.1 0.6 -0.4 0.7 -0.5 -1.7 -2.0 0.1 -0.7 2.0 0.2 -2.3 -0.5 -1.6 -1.5
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 3.6 2.6 6.4 5.9 6.5 9.6 9.3 9.5 9.9 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 3.4 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 3.9 0 0 2.9 3.2 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 6.9757 8.571 8.0018 7.7366 7.039 8.4161 8.2468 10.788 9.9278 10.1286 9.1261 10.3438 7.0997 7.9451 7.6627 7.3758 7.0965 7.6918
Actin 0.0325 0.0065 0.0275 0.0327 0.0517 0.0205 0.0273 0.0123 0.0228 0.0104 0.0442 0.0106 0.0373 0.0256 0.0289 0.017 0.0326 0.0367
Bud 0.0324 0.0381 0.199 0.1275 0.063 0.0094 0.0389 0.0398 0.239 0.1784 0.1238 0.0126 0.0454 0.0285 0.2215 0.1331 0.1098 0.0163
Bud Neck 0.0217 0.0071 0.0093 0.003 0.0284 0.2541 0.0295 0.0045 0.0054 0.0336 0.0052 0.3009 0.0166 0.0054 0.0049 0.0174 0.0354 0.2093
Bud Periphery 0.1049 0.0607 0.4297 0.4843 0.3073 0.0441 0.0988 0.044 0.3443 0.492 0.4043 0.0419 0.1114 0.0464 0.4044 0.4619 0.4465 0.0505
Bud Site 0.1497 0.2413 0.0424 0.006 0.0091 0.0101 0.1316 0.2046 0.0339 0.0066 0.0232 0.0125 0.1271 0.306 0.0483 0.0133 0.0152 0.0266
Cell Periphery 0.0958 0.0313 0.0186 0.0346 0.0672 0.0352 0.0569 0.0162 0.0113 0.0233 0.031 0.0229 0.1166 0.0145 0.0146 0.0286 0.0442 0.0309
Cytoplasm 0.4399 0.5762 0.2448 0.1892 0.2978 0.5812 0.4601 0.6524 0.2936 0.2335 0.2966 0.5491 0.4821 0.5511 0.2633 0.2898 0.3075 0.5934
Cytoplasmic Foci 0.0141 0.0032 0.0018 0.0021 0.0056 0.0073 0.0132 0.007 0.0053 0.0013 0.0031 0.0099 0.0082 0.0028 0.0014 0.0008 0.0009 0.0072
Eisosomes 0.0005 0.0001 0.0001 0.0003 0.0011 0.0001 0.0006 0 0.0001 0 0.0002 0 0.0004 0.0001 0 0 0.0001 0.0014
Endoplasmic Reticulum 0.0093 0.0048 0.0023 0.0058 0.0124 0.0078 0.0214 0.0017 0.0017 0.0017 0.0046 0.0063 0.0144 0.0029 0.0019 0.0014 0.0014 0.0057
Endosome 0.0168 0.0025 0.006 0.0083 0.0576 0.0074 0.0302 0.0028 0.0174 0.0007 0.0289 0.0091 0.0075 0.0015 0.0016 0.0026 0.0005 0.0035
Golgi 0.0072 0.0015 0.0034 0.0079 0.0095 0.0034 0.0119 0.001 0.007 0.0002 0.005 0.0054 0.0038 0.001 0.0008 0.0004 0.0005 0.0047
Lipid Particles 0.0096 0.0006 0.0005 0.0035 0.0025 0.0006 0.0189 0.0002 0.001 0.0001 0.0005 0.0019 0.0028 0.0002 0.0001 0 0.0001 0.0011
Mitochondria 0.0176 0.0004 0.0011 0.048 0.0442 0.0062 0.012 0.0002 0.0042 0.0002 0.0039 0.0029 0.0033 0.0002 0.0004 0.0002 0.0005 0.0008
None 0.0078 0.0007 0.0005 0.0005 0.0073 0.0006 0.0057 0.0007 0.0007 0.0071 0.0091 0.0007 0.0027 0.0013 0.0007 0.0003 0.0004 0.0012
Nuclear Periphery 0.0039 0.0008 0.001 0.0011 0.0027 0.0004 0.0084 0.0005 0.0007 0.0009 0.0013 0.001 0.0023 0.0006 0.0005 0.0006 0.0003 0.0003
Nucleolus 0.0013 0.0007 0.0001 0.0002 0.0003 0.0002 0.0014 0.0002 0.0001 0.0001 0.0001 0.0008 0.0002 0.0001 0.0001 0.0001 0.0001 0.0002
Nucleus 0.004 0.0022 0.0014 0.0011 0.0019 0.001 0.0053 0.0022 0.0014 0.0016 0.0016 0.0014 0.0014 0.0016 0.0009 0.0017 0.0005 0.0009
Peroxisomes 0.004 0.0001 0.0004 0.0012 0.0007 0.0004 0.0056 0.0002 0.0008 0 0.0002 0.0015 0.0021 0.0001 0.0001 0 0.0001 0.0009
Punctate Nuclear 0.0035 0.0001 0.0001 0.0002 0.0003 0.0001 0.0043 0.0004 0.0002 0.0001 0.0003 0.0009 0.0025 0.0002 0.0001 0 0 0.0002
Vacuole 0.0217 0.02 0.0089 0.0399 0.0268 0.0089 0.0159 0.0088 0.008 0.0076 0.0115 0.0069 0.0115 0.0093 0.0053 0.0277 0.0032 0.0074
Vacuole Periphery 0.0019 0.0011 0.001 0.0026 0.0027 0.0008 0.0021 0.0005 0.0011 0.0007 0.0017 0.0008 0.0006 0.0005 0.0004 0.0027 0.0002 0.0007

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 28.3677 27.9823 32.4439 33.9753 24.6045 21.1173 34.924 31.508 30.7757 35.6967
Translational Efficiency 1.2335 1.2037 1.0063 0.9634 1.2666 1.7081 0.947 1.0387 1.0193 0.8584

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Cdc42p-activated signal transducing kinase; involved in pheromone response, pseudohyphal/invasive growth, vacuole inheritance, down-regulation of sterol uptake; GBB motif binds Ste4p; member of the PAK (p21-activated kinase) family
Localization
Cell Percentages cytoplasm (82%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-5

Ste20

Ste20


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ste20-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available