ORF
Human Ortholog
Description Putative protein of unknown function; may be involved in the uptake of chloride ions; does not appear to be involved in monocarboxylic acid transport; green fluorescent protein (GFP)-fusion protein localizes to the vacuole
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.05 0 0 0 0 0 0 0 0 0 0 0.13 0.13 0.11 0 0 0 0.05 0.1 0.19 0.1 0.09 0.05
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.69 0.72 0.81 0.88 0.86 0.83 0.82 0.8 0.78 0.81 0.85 0.79 0.8 0.79 0.81 0.84 0.73 0.73 0 0 0 0 0 0
Endoplasmic Reticulum 0.06 0.18 0.05 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0.06 0.05 0 0.07 0.12 0.08 0.08 0.09 0.1
Endosome 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.07 0.06 0.08 0.08 0.07 0.09
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.15 0.14 0.09 0.15 0.14 0.13 0.18 0 0 0 0.05 0 0 0.05 0.06 0.1 0.15 0.1 0.07
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.23 0.05 0.06 0.06 0.15 0.14 0.2 0.18 0.24 0.23 0.12 0.18 0 0 0 0.14 0.25 0.24 0.68 0.54 0.42 0.48 0.56 0.56
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 9 25 17 5 2 1 2 2 2 1 0 1 164 175 150 2 3 5 22 56 94 47 64 39
Bud 4 14 8 6 2 5 9 11 11 5 5 8 23 23 9 1 2 0 3 2 1 0 6 10
Bud Neck 0 0 1 1 1 0 0 1 0 0 0 0 24 22 30 2 0 0 6 23 15 7 13 12
Bud Site 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 7 6 0 2 0 0 1 0 0 0 0 51 51 44 1 1 2 0 5 6 3 4 4
Cytoplasm 233 344 312 272 302 253 338 382 268 405 267 379 1039 1039 1060 387 294 149 12 14 8 12 15 12
Endoplasmic Reticulum 19 86 19 12 3 2 2 1 2 1 6 1 59 65 53 27 20 8 31 70 38 40 69 77
Endosome 16 9 17 1 5 2 1 1 0 0 0 0 7 8 0 5 15 10 29 32 38 38 52 68
Golgi 1 1 0 1 1 0 0 0 0 0 0 0 1 1 0 0 2 1 7 13 11 7 12 33
Mitochondria 6 4 7 11 5 47 57 43 52 69 42 84 1 1 0 24 15 3 19 35 51 72 73 57
Nucleus 2 0 0 1 1 3 5 11 11 9 6 14 2 2 5 7 8 9 0 0 0 0 1 0
Nuclear Periphery 1 1 0 0 0 1 1 0 1 2 1 3 5 1 0 0 1 0 0 0 2 0 1 3
Nucleolus 0 0 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 3 1 4 3 0 0 0 0 0 1 4 3 2 0 4 1 1 1 9 11 3 3
SpindlePole 3 1 7 2 3 2 1 1 0 1 0 0 47 56 71 2 2 2 2 0 1 2 2 7
Vac/Vac Membrane 79 23 25 20 53 42 81 87 81 116 38 87 8 9 4 67 100 50 298 305 207 229 425 439
Unique Cell Count 339 477 386 308 350 306 412 480 342 502 315 477 1306 1317 1309 463 404 205 441 564 494 479 758 786
Labelled Cell Count 374 515 423 333 384 362 497 542 428 609 365 578 1435 1456 1428 525 467 240 441 564 494 479 758 786


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.5 5.8 4.7 4.5 4.8 3.9 3.9 4.4 3.9 3.9 3.9 3.8 6.4 6.4 6.5 5.1 6.0 6.3 6.3 6.2 6.7
Std Deviation (1e-4) 0.6 0.7 0.9 1.2 1.3 1.3 1.1 1.2 1.1 1.1 1.0 0.9 1.0 1.0 1.1 1.1 1.2 1.3 1.1 1.3 1.6
Intensity Change (Log2) – – – -0.07 0.0 -0.27 -0.28 -0.12 -0.27 -0.3 -0.29 -0.34 0.44 0.44 0.46 0.1 0.34 0.41 0.41 0.39 0.49


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 4.6 4.9 4.1 -3.9 -3.3 -1.2
Bud 0 0 0 0 0 0 0 0 0 -0.4 -0.4 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 2.3 2.1 2.6 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 2.2 2.2 1.8 0 0 0
Cytoplasm 2.7 2.0 0.6 0.4 -0.5 -0.8 -0.1 1.4 -0.5 -0.5 -0.8 0.1 1.0 -2.7 -2.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 -0.3 0 -0.7 0.6 0 -0.6
Endosome 0 0 0 0 0 0 0 0 0 -5.6 -5.5 0 -3.0 -0.5 0.3
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 2.6 1.6 0
Nucleus 0 0 0 0 0 3.6 0 0 3.4 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 1.8 2.2 3.0 0 0 0
Vacuole 0 3.8 3.2 5.5 5.1 6.6 6.7 2.6 5.1 -7.3 -7.2 -8.2 3.7 7.0 6.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.303 1.8794 1.8491 1.7673 1.6807 1.6233 3.3222 4.1897 3.7128 3.4109 3.3602 4.0818 4.6025 4.793 5.072 6.0699 4.736 4.7153
Actin 0.0126 0.0128 0.0068 0.0076 0.0025 0.0095 0.0141 0.0005 0.0112 0.0066 0.0027 0.0011 0.0043 0.0014 0.0106 0.003 0.0002 0.0023
Bud 0.0021 0.0051 0.0099 0.0033 0.0245 0.0013 0.0011 0.002 0.002 0.002 0.002 0.0007 0.0026 0.0038 0.0025 0.0049 0.0007 0.0007
Bud Neck 0.0036 0.0037 0.0045 0.0219 0.0024 0.0145 0.0025 0.0019 0.0046 0.0025 0.0086 0.0068 0.0014 0.002 0.003 0.0079 0.0029 0.0035
Bud Periphery 0.0032 0.0049 0.0085 0.0146 0.0175 0.0042 0.0027 0.0028 0.0033 0.0066 0.0037 0.0024 0.0126 0.0093 0.0102 0.0292 0.0013 0.0037
Bud Site 0.0218 0.0392 0.0197 0.0015 0.0065 0.0018 0.0175 0.0255 0.0129 0.0023 0.0077 0.0012 0.0043 0.012 0.0067 0.0021 0.0018 0.0008
Cell Periphery 0.0052 0.004 0.003 0.0023 0.0045 0.0024 0.0089 0.0034 0.0031 0.003 0.0031 0.0024 0.0079 0.0055 0.0068 0.0108 0.0015 0.0035
Cytoplasm 0.2655 0.2891 0.2783 0.2779 0.3248 0.3104 0.2711 0.3126 0.2517 0.2902 0.3336 0.2989 0.2409 0.331 0.2637 0.2257 0.2624 0.3499
Cytoplasmic Foci 0.0622 0.0853 0.0798 0.0749 0.1011 0.0976 0.0968 0.0585 0.0892 0.0998 0.0813 0.0908 0.0694 0.0351 0.0525 0.0227 0.048 0.0571
Eisosomes 0.0014 0.0012 0.0005 0.0001 0.0003 0.0002 0.0007 0.0002 0.0003 0.0004 0.0003 0.0001 0.0006 0.0002 0.0003 0.0002 0 0.0002
Endoplasmic Reticulum 0.0218 0.0175 0.0303 0.0161 0.0206 0.023 0.0241 0.0299 0.0251 0.0173 0.0139 0.0324 0.2365 0.1556 0.2228 0.3276 0.2035 0.1861
Endosome 0.1521 0.1811 0.2015 0.3446 0.1659 0.1726 0.1392 0.1765 0.195 0.2007 0.2368 0.1825 0.1651 0.0997 0.1364 0.1436 0.2104 0.1289
Golgi 0.0073 0.0202 0.0142 0.0281 0.0108 0.0108 0.0243 0.0094 0.0185 0.0241 0.0153 0.0073 0.0141 0.0067 0.011 0.0066 0.0045 0.006
Lipid Particles 0.021 0.0142 0.0139 0.0095 0.0124 0.0188 0.0301 0.0203 0.0151 0.0192 0.0086 0.024 0.0303 0.0119 0.0197 0.0099 0.0052 0.0094
Mitochondria 0.0202 0.0075 0.016 0.0066 0.0105 0.0101 0.0189 0.0051 0.0099 0.008 0.0152 0.0035 0.0084 0.003 0.0041 0.0031 0.0032 0.0038
None 0.2916 0.2242 0.2217 0.0622 0.201 0.1881 0.2684 0.254 0.2337 0.2217 0.1147 0.1836 0.1277 0.1858 0.16 0.1051 0.0855 0.1295
Nuclear Periphery 0.0047 0.0046 0.0051 0.0053 0.0037 0.0055 0.0029 0.0034 0.0052 0.0064 0.0024 0.0033 0.0062 0.0076 0.0083 0.0064 0.0044 0.0059
Nucleolus 0.0015 0.0033 0.0025 0.0006 0.0043 0.0016 0.0014 0.0017 0.0016 0.0012 0.0008 0.001 0.0006 0.0014 0.0009 0.0005 0.0007 0.0005
Nucleus 0.0057 0.0085 0.0054 0.0027 0.0067 0.006 0.0032 0.0054 0.0044 0.0032 0.0028 0.004 0.0021 0.0073 0.0036 0.0026 0.0056 0.003
Peroxisomes 0.0016 0.0052 0.0055 0.0014 0.0018 0.0037 0.0026 0.0013 0.0057 0.0053 0.0015 0.0053 0.0019 0.0005 0.0014 0.0002 0.0021 0.0037
Punctate Nuclear 0.0023 0.0081 0.0041 0.0013 0.0047 0.0053 0.0044 0.0013 0.0019 0.0022 0.0012 0.0017 0.0011 0.0014 0.0015 0.0007 0.0011 0.0008
Vacuole 0.0864 0.0555 0.0621 0.0991 0.0684 0.1044 0.0618 0.0805 0.0987 0.0711 0.1332 0.1394 0.0573 0.1135 0.0677 0.0787 0.1478 0.0943
Vacuole Periphery 0.0063 0.005 0.0067 0.0184 0.0051 0.0083 0.0034 0.0039 0.0068 0.0061 0.0106 0.0075 0.0048 0.0052 0.0063 0.0087 0.007 0.0066

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.8131 15.1831 15.1515 20.3892 15.9463 11.1216 16.1456 19.4721 17.904 16.4093
Translational Efficiency 0.3104 0.4468 0.5786 0.4328 0.2962 0.5246 0.3765 0.324 0.4149 0.3139

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
ORF
Human Ortholog
Description Putative protein of unknown function; may be involved in the uptake of chloride ions; does not appear to be involved in monocarboxylic acid transport; green fluorescent protein (GFP)-fusion protein localizes to the vacuole
Localization
Cell Percentages vacuole (38%)
Cell Cycle Regulation No
Subcompartmental Group N/A

YHL008C

YHL008C


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yhl008c-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available