Standard name
Human Ortholog
Description Ribosomal 60S subunit protein L8A; required for processing of 27SA3 pre-rRNA to 27SB pre-rRNA during assembly of large ribosomal subunit; depletion leads to a turnover of pre-rRNA; L8 binds to Domain I of 25S and 5.8 S rRNAs; mutation results in decreased amounts of free 60S subunits; homologous to mammalian ribosomal protein L7A, no bacterial homolog; RPL8A has a paralog, RPL8B, that arose from the whole genome duplication
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.11 0.12 0 0.07 0 0 0 0 0.07 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 0.97 0.99 1.0 1.0 0.97 0.96 0.93 0.92 0.9 0.89 0.87 1.0 1.0 1.0 1.0 1.0 0.99 0.93 0.96 0.96 0.96 0.95 0.95
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0.05 0.08 0.07 0.06 0 0 0 0 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Bud 0 1 0 0 0 0 0 1 0 1 0 2 0 0 0 0 0 0 0 0 0 0 1 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 4 11 3 6 0 6 3 9 8 3 5 6 0 1 3 0 1 6 0 0 0 0 0 0
Cytoplasm 38 85 70 84 114 135 157 186 108 162 54 121 116 235 348 96 108 174 56 90 77 56 196 210
Endoplasmic Reticulum 0 0 0 0 0 1 7 9 6 14 4 9 0 0 0 2 5 7 0 0 0 0 1 1
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 1 0 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 1 2
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0 1 0 0 2 1
Unique Cell Count 38 88 71 84 114 139 164 201 118 180 61 139 116 235 348 96 108 175 61 94 80 59 208 222
Labelled Cell Count 42 97 73 90 114 143 167 206 122 184 64 139 116 236 351 98 114 188 61 94 80 59 208 222


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 432.5 440.5 412.9 408.0 336.7 228.7 175.4 155.0 133.3 129.2 120.8 119.7 369.8 356.9 339.9 250.1 345.2 415.0 359.8 422.0 414.8
Std Deviation (1e-4) 73.0 68.8 75.0 87.9 71.5 64.0 51.5 42.1 35.8 34.0 31.2 35.2 107.2 75.7 74.2 69.1 70.9 98.8 107.6 109.6 149.0
Intensity Change (Log2) – – – -0.02 -0.29 -0.85 -1.24 -1.41 -1.63 -1.68 -1.77 -1.79 -0.16 -0.21 -0.28 -0.72 -0.26 0.01 -0.2 0.03 0.01


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.1 1.3 -0.7 -1.1 -1.9 -2.0 -2.3 -2.4 -2.8 1.3 1.8 2.2 1.2 1.2 0.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.002 0.0019 0.0005 0.0004 0.0414 0.0068 0.0038 0.0012 0.0015 0.0007 0.1283 0.0008 0.0298 0.0011 0.0065 0.0535 0.0005 0.016
Bud 0.0022 0.0024 0.0024 0.0013 0.0039 0.0074 0.0053 0.0047 0.0059 0.0066 0.0065 0.0041 0.0056 0.005 0.0077 0.0034 0.0045 0.0084
Bud Neck 0.0014 0.0011 0.0013 0.0012 0.0312 0.0064 0.0009 0.0008 0.0012 0.0011 0.0018 0.0031 0.0099 0.0005 0.0016 0.0033 0.0018 0.002
Bud Periphery 0.0041 0.001 0.0019 0.0007 0.0066 0.0079 0.0052 0.0031 0.0019 0.0019 0.0053 0.0021 0.0048 0.0012 0.0026 0.0014 0.0008 0.0041
Bud Site 0.0012 0.002 0.0008 0.0004 0.0305 0.0011 0.0033 0.0017 0.0019 0.0007 0.0027 0.0004 0.0149 0.0016 0.0069 0.0031 0.0002 0.001
Cell Periphery 0.0914 0.0188 0.0647 0.047 0.0456 0.0564 0.0264 0.0123 0.0132 0.0105 0.0021 0.0044 0.0351 0.0066 0.0116 0.0023 0.0008 0.0028
Cytoplasm 0.4172 0.8265 0.6422 0.5177 0.2331 0.4356 0.6456 0.8925 0.8105 0.6936 0.5721 0.7887 0.5356 0.8836 0.7708 0.4325 0.8867 0.351
Cytoplasmic Foci 0.0059 0.0018 0.0073 0.0007 0.007 0.0016 0.0013 0.0004 0.0043 0.0012 0.0064 0.0007 0.0118 0.0005 0.0022 0.0622 0.0004 0.0011
Eisosomes 0.0009 0.0006 0.0002 0 0.0051 0.0009 0.0009 0 0.0002 0 0.0005 0 0.0019 0.0001 0.0004 0.0053 0 0.0007
Endoplasmic Reticulum 0.0341 0.0032 0.0071 0.0025 0.0094 0.0178 0.0108 0.001 0.0035 0.0006 0.0085 0.0023 0.0061 0.0005 0.001 0.0087 0.0008 0.0052
Endosome 0.0087 0.0027 0.002 0.0021 0.006 0.0061 0.0066 0.0004 0.0006 0.0013 0.0407 0.0016 0.0026 0.0002 0.0019 0.0434 0.0006 0.0026
Golgi 0.0012 0.0009 0.0002 0.0001 0.003 0.0012 0.0025 0.0001 0.0001 0.0001 0.0339 0.0001 0.003 0.0001 0.0014 0.0211 0 0.0013
Lipid Particles 0.0078 0.0018 0.0021 0.0013 0.0056 0.003 0.001 0.0001 0.0006 0.0003 0.0084 0.0001 0.003 0.0001 0.0029 0.0537 0 0.0003
Mitochondria 0.0263 0.0099 0.0087 0.0017 0.0484 0.0411 0.0393 0.0035 0.0226 0.0038 0.0123 0.003 0.0846 0.0185 0.051 0.039 0.0016 0.3674
None 0.009 0.0016 0.002 0.0006 0.0114 0.003 0.0033 0.0006 0.0054 0.0015 0.0012 0.0007 0.0111 0.0013 0.0035 0.0623 0.0003 0.0047
Nuclear Periphery 0.0876 0.0162 0.024 0.0208 0.0503 0.0694 0.0422 0.0073 0.0158 0.0166 0.011 0.0256 0.0513 0.0077 0.0195 0.0332 0.0094 0.1137
Nucleolus 0.0276 0.0046 0.0164 0.0177 0.1764 0.0053 0.0061 0.0007 0.0259 0.0053 0.0006 0.0016 0.0175 0.0025 0.0131 0.0567 0.0002 0.003
Nucleus 0.0304 0.0168 0.0196 0.025 0.066 0.0382 0.0615 0.0177 0.0398 0.0684 0.015 0.0476 0.0984 0.051 0.0693 0.0515 0.0417 0.0631
Peroxisomes 0.0015 0.0005 0.0003 0.0001 0.0036 0.0006 0.0006 0.0002 0.0007 0.0003 0.0012 0.0002 0.0077 0.0004 0.003 0.0248 0.0001 0.0018
Punctate Nuclear 0.0024 0.0002 0.0003 0.0001 0.0021 0.0003 0.0002 0 0.0002 0.0001 0.0009 0.0001 0.0014 0.0001 0.0006 0.0189 0 0.0004
Vacuole 0.1692 0.0636 0.1558 0.2874 0.1135 0.1645 0.0835 0.0408 0.0324 0.1208 0.1041 0.069 0.0473 0.0107 0.0137 0.0095 0.0362 0.008
Vacuole Periphery 0.0678 0.0217 0.0402 0.0713 0.1 0.1254 0.0498 0.0108 0.012 0.0646 0.0365 0.0438 0.0166 0.0068 0.0089 0.01 0.0133 0.0415

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 1237.9311 1422.6333 1856.8901 1425.7014 1639.359 1504.7197 1323.9443 1264.1856 1468.1287 1226.3579
Translational Efficiency 1.4235 1.4813 1.3362 1.6374 1.4616 1.1897 1.5118 1.6909 1.4182 1.6646

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Ribosomal 60S subunit protein L8A; required for processing of 27SA3 pre-rRNA to 27SB pre-rRNA during assembly of large ribosomal subunit; depletion leads to a turnover of pre-rRNA; L8 binds to Domain I of 25S and 5.8 S rRNAs; mutation results in decreased amounts of free 60S subunits; homologous to mammalian ribosomal protein L7A, no bacterial homolog; RPL8A has a paralog, RPL8B, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (98%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-1

Rpl8a

Rpl8a


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rpl8a-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available