Standard name
Human Ortholog
Description Protein that binds eIF4G and has a role in repression of translation; has an RGG motif; found in cytoplasmic P bodies; binds to mRNAs under glucose starvation stress, most often in the 5' UTR; found associated with small nucleolar RNAs snR10 and snR11; SBP1 has a paralog, RNP1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0.07 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0.05 0 0 0.06 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.98 0.95 0.98 0.95 0.91 0.8 0.68 0.77 0.67 0.67 0.6 0.94 0.9 0.95 0.98 0.98 0.96 0.96 0.93 0.92 0.93 0.95 0.89
Endoplasmic Reticulum 0 0 0.06 0 0.06 0.09 0.16 0.22 0.14 0.18 0.21 0.24 0.05 0.11 0 0 0 0 0 0 0.05 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0.08 0.06 0.13 0.1 0.11 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.06 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 1 0 1 1 4 11 16 13 12 7 0 0 2 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 2 0 0 1 0 1 2 0 0 0 2 0 0 1 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 3 8 3 4 4 6 16 10 13 12 19 1 1 2 4 0 2 0 0 2 0 1 1
Cytoplasm 123 207 189 147 208 174 228 202 173 197 129 150 150 188 288 260 279 345 117 192 180 93 228 220
Endoplasmic Reticulum 3 1 11 3 14 17 45 66 31 54 40 60 8 22 13 4 10 16 1 1 10 2 3 9
Endosome 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 1 2 10 25 14 38 19 28 1 5 10 1 1 9 0 0 0 0 0 1
Mitochondria 0 1 0 0 0 1 4 2 13 7 9 3 0 0 0 10 5 4 0 0 0 0 0 1
Nucleus 0 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 1 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 1 1 2 0 2 1 12 0 2 1 1 4 4 0 3 2 2 1 4
Unique Cell Count 125 211 198 150 219 192 284 296 224 292 193 251 159 209 302 266 284 360 122 206 197 100 240 247
Labelled Cell Count 126 215 210 153 228 200 298 325 257 326 222 279 161 218 317 282 299 380 122 206 197 100 240 247


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 57.4 46.4 45.7 42.4 49.0 42.3 43.2 40.8 38.4 41.6 40.0 43.3 45.8 50.3 55.1 48.3 62.4 67.5 49.4 51.1 55.4
Std Deviation (1e-4) 12.8 11.5 7.8 10.1 9.6 8.5 9.7 8.6 8.9 10.4 10.2 13.0 7.5 12.3 12.8 15.9 23.0 23.8 14.2 12.8 14.7
Intensity Change (Log2) – – – -0.11 0.1 -0.11 -0.08 -0.17 -0.25 -0.14 -0.19 -0.08 0.0 0.14 0.27 0.08 0.45 0.56 0.11 0.16 0.28


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 3.5 2.6 3.2 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 -1.4 -1.1 -1.2 0.7 0.2 0.2 1.0 1.6 0 0 0 -1.7 0 0
Cytoplasm 1.3 -0.2 -1.9 -4.8 -7.3 -5.3 -7.4 -7.3 -8.7 -0.5 -2.1 0 1.4 1.8 0.2
Endoplasmic Reticulum -1.7 0.4 1.3 3.5 5.0 2.8 4.1 4.5 5.3 -0.2 1.8 -0.6 -2.4 -1.1 -0.6
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 4.2 3.6 5.3 4.5 4.9 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 3.4 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 3.1 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 44.4841 53.0722 45.8594 47.2959 30.6749 49.8019 42.2387 42.9496 42.0487 38.1815 37.5389 44.1961 39.942 48.5653 47.0021 43.0032 37.9615 44.5682
Actin 0.0088 0.0009 0.0157 0.0015 0.0356 0.0019 0.0048 0.022 0.0206 0.0004 0.002 0.0046 0.0185 0.0009 0.0042 0.0024 0.011 0.0023
Bud 0.0015 0.0015 0.0014 0.0053 0.0034 0.0013 0.0015 0.0061 0.0069 0.0013 0.0033 0.0024 0.001 0.0007 0.0013 0.002 0.001 0.0013
Bud Neck 0.0008 0.0009 0.0013 0.0009 0.0008 0.002 0.0009 0.0008 0.0009 0.0004 0.0005 0.0011 0.0013 0.0005 0.0005 0.0008 0.001 0.0011
Bud Periphery 0.0016 0.001 0.0009 0.0027 0.0032 0.0014 0.0011 0.004 0.0106 0.0002 0.0013 0.0011 0.0009 0.0003 0.0006 0.0017 0.0005 0.0005
Bud Site 0.0007 0.001 0.0032 0.0005 0.0019 0.0002 0.0008 0.0177 0.0056 0.0005 0.0004 0.0004 0.0011 0.0005 0.0004 0.0004 0.0055 0.0001
Cell Periphery 0.0026 0.0023 0.0014 0.0022 0.0018 0.0013 0.0027 0.0045 0.0014 0.0002 0.0004 0.0003 0.0025 0.0007 0.0016 0.0031 0.0005 0.0002
Cytoplasm 0.7183 0.9424 0.8145 0.6633 0.6425 0.7605 0.7523 0.859 0.7988 0.8291 0.8923 0.7317 0.7545 0.9476 0.8788 0.6783 0.7962 0.8381
Cytoplasmic Foci 0.0054 0.0023 0.0081 0.0241 0.0254 0.0084 0.0048 0.0039 0.0069 0.0074 0.0184 0.0286 0.0044 0.0032 0.013 0.0136 0.0128 0.0101
Eisosomes 0.0001 0 0.0002 0.0001 0.0002 0 0.0001 0.0007 0.0001 0 0 0 0.0002 0 0 0 0.0012 0
Endoplasmic Reticulum 0.0926 0.018 0.0178 0.0201 0.0069 0.0917 0.0709 0.0218 0.0169 0.0116 0.0055 0.0388 0.0729 0.0156 0.0101 0.0238 0.0026 0.0715
Endosome 0.0287 0.0043 0.0198 0.0329 0.0389 0.0405 0.0318 0.0072 0.025 0.0272 0.0368 0.0804 0.0206 0.0082 0.0215 0.0827 0.0448 0.0392
Golgi 0.0032 0.0012 0.0051 0.0035 0.0476 0.0077 0.0093 0.0049 0.0038 0.0005 0.002 0.0228 0.005 0.0008 0.0035 0.0051 0.0025 0.0062
Lipid Particles 0.0012 0.0003 0.0024 0.017 0.0153 0.0002 0.0009 0.0002 0.0021 0.0002 0.0004 0.0047 0.0022 0.0002 0.0034 0.0037 0.0027 0.0002
Mitochondria 0.0087 0.0014 0.0134 0.0065 0.0803 0.0104 0.0056 0.0273 0.004 0.0005 0.0013 0.0273 0.0136 0.0006 0.0025 0.0019 0.028 0.0042
None 0.0024 0.0003 0.0013 0.0047 0.002 0.0005 0.0015 0.0004 0.0163 0.0008 0.0013 0.0031 0.0019 0.0002 0.0009 0.0003 0.0027 0.0005
Nuclear Periphery 0.0228 0.0018 0.0062 0.0098 0.0058 0.0199 0.014 0.0026 0.0102 0.0199 0.0022 0.0065 0.0268 0.0019 0.0055 0.0107 0.0036 0.0106
Nucleolus 0.0002 0.0001 0.0003 0.0004 0.0021 0 0.0001 0 0.0005 0.0001 0.0001 0.0002 0.0033 0 0.0003 0.0002 0.022 0
Nucleus 0.0047 0.0013 0.002 0.0032 0.0061 0.0022 0.0044 0.0011 0.0054 0.0123 0.0017 0.002 0.0091 0.001 0.0018 0.0028 0.0102 0.0019
Peroxisomes 0.0004 0.0001 0.0051 0.0012 0.0147 0.0001 0.0001 0.0004 0.0013 0.0001 0.0002 0.0006 0.0005 0.0001 0.0011 0.0001 0.0043 0.0001
Punctate Nuclear 0.0003 0 0.0003 0.0052 0.0005 0.0001 0.0001 0.0001 0.0031 0.0012 0.0002 0.0028 0.0072 0 0.0008 0.0001 0.004 0.0001
Vacuole 0.0713 0.016 0.0596 0.1571 0.0473 0.033 0.0688 0.0122 0.042 0.0676 0.022 0.0278 0.043 0.0146 0.0378 0.1184 0.031 0.0075
Vacuole Periphery 0.0233 0.0031 0.0201 0.0378 0.0176 0.0165 0.0234 0.0033 0.0175 0.0186 0.0079 0.0127 0.0098 0.0025 0.0106 0.0478 0.012 0.0042

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 224.4212 189.4734 176.1603 217.0244 244.9014 223.0509 232.1455 233.1223 261.4405 216.1563
Translational Efficiency 3.63 4.0437 3.208 2.6193 2.7676 4.2665 2.7787 2.634 2.3566 3.0474

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein that binds eIF4G and has a role in repression of translation; has an RGG motif; found in cytoplasmic P bodies; binds to mRNAs under glucose starvation stress, most often in the 5' UTR; found associated with small nucleolar RNAs snR10 and snR11; SBP1 has a paralog, RNP1, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-2

Sbp1

Sbp1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sbp1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available