Standard name
Human Ortholog
Description Probable catalytic subunit of Nem1p-Spo7p phosphatase holoenzyme; regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology and sporulation; homolog of the human protein Dullard
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.05 0.05 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0.06 0.08 0.07 0.1 0.06 0.06 0.1
Bud 0.08 0 0.09 0.07 0.1 0.13 0.19 0.15 0.15 0.17 0.14 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0.06 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0.06 0.07 0 0.11 0.07 0.06 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.56 0.54 0.54 0.51 0.49 0.33 0.23 0.21 0.15 0.15 0.17 0.19 0.68 0.75 0.74 0.56 0.51 0.5 0.16 0.28 0.1 0.41 0.33 0.28
Endoplasmic Reticulum 0 0.13 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.07 0.07 0.13 0.07 0.12 0 0 0
Endosome 0.05 0.17 0 0.07 0.09 0 0.05 0.09 0.07 0 0.05 0.05 0 0 0 0.14 0.11 0.17 0.09 0.07 0.11 0.06 0.1 0.1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.23 0.06 0.08 0.14 0.16 0.45 0.36 0.3 0.39 0.34 0.44 0.38 0 0 0 0 0 0 0.21 0.19 0.25 0.13 0.17 0.11
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0.06 0 0 0.05 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.06 0.06 0 0.12 0.07 0.1 0.18 0.16 0.13 0.12 0.16 0.11 0 0 0 0.05 0.05 0.05 0 0 0.07 0.05 0 0.09
SpindlePole 0.13 0.13 0.27 0.23 0.2 0.21 0.37 0.44 0.36 0.46 0.35 0.41 0.22 0.19 0.21 0.22 0.29 0.23 0.14 0.18 0.15 0.16 0.15 0.16
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.05 0.05 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 3 4 7 1 3 3 0 2 1 5 0 0 20 9 4 4 8 10 7 7 17 9 15 35
Bud 7 2 13 10 14 18 41 43 35 50 39 29 10 7 2 4 10 2 1 2 1 1 4 8
Bud Neck 1 2 5 3 2 2 3 2 5 5 4 1 16 12 7 2 11 4 5 4 2 5 8 13
Bud Site 1 0 5 1 2 4 13 20 8 33 20 24 1 0 1 0 0 0 – – – – – –
Cell Periphery 0 0 1 1 1 2 0 1 0 1 1 0 3 5 2 5 10 4 2 0 3 2 1 5
Cytoplasm 48 42 76 74 65 45 48 59 35 44 46 73 167 201 219 82 150 87 16 32 17 72 84 96
Endoplasmic Reticulum 3 10 2 2 2 3 3 0 4 0 1 4 4 1 1 7 21 12 13 8 19 6 7 8
Endosome 4 13 4 10 12 2 10 26 17 7 14 19 0 4 9 21 32 29 9 8 18 10 25 33
Golgi 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0 0 1 3 1 0 2 0 0 1
Mitochondria 20 5 11 21 22 61 76 84 89 100 122 144 2 0 1 5 8 2 22 22 42 22 42 39
Nucleus 0 1 0 0 0 0 0 1 2 2 2 3 3 0 0 0 1 2 0 0 0 0 2 1
Nuclear Periphery 0 0 1 0 0 0 0 0 0 0 2 1 0 0 1 0 2 0 0 0 2 0 0 0
Nucleolus 0 0 1 0 0 0 9 18 6 10 13 21 0 0 0 0 4 2 0 0 0 0 1 0
Peroxisomes 5 5 5 17 9 14 38 46 30 34 43 41 2 0 4 7 16 8 3 4 12 8 10 31
SpindlePole 11 10 39 34 27 29 78 125 83 136 97 157 55 50 62 32 86 39 14 20 25 28 37 55
Vac/Vac Membrane 0 2 1 0 2 0 1 7 7 1 6 12 1 0 1 11 14 9 4 2 2 3 8 6
Unique Cell Count 86 78 142 145 134 137 212 281 231 295 276 381 246 268 295 146 295 173 104 116 172 177 253 342
Labelled Cell Count 103 96 172 174 161 183 320 434 322 428 410 529 285 289 314 180 374 213 104 116 172 177 253 342


Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.4 4.7 4.2 4.0 4.5 3.8 3.9 4.1 3.9 3.7 3.5 3.6 5.6 5.6 5.6 6.5 6.5 6.6 5.8 6.0 6.0
Std Deviation (1e-4) 0.4 0.6 1.4 1.4 1.8 1.4 1.3 1.8 1.5 1.0 0.9 0.9 1.2 1.1 1.1 1.1 1.5 1.4 1.8 1.7 1.4
Intensity Change (Log2) – – – -0.07 0.07 -0.15 -0.13 -0.06 -0.13 -0.21 -0.27 -0.24 0.4 0.41 0.41 0.62 0.62 0.63 0.44 0.5 0.49


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 – –
Bud Neck – – – – – – – – – – – – 0 – –
Bud Site – – – – – – – – – – – – 0 – –
Cell Periphery – – – – – – – – – – – – 0 – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 – –
Endosome – – – – – – – – – – – – 0 – –
Golgi – – – – – – – – – – – – 0 – –
Mitochondria – – – – – – – – – – – – 0 – –
Nuclear Periphery – – – – – – – – – – – – 0 – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 – –
Peroxisomes – – – – – – – – – – – – 0 – –
SpindlePole – – – – – – – – – – – – 0 – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 – –
Cytoplasm – – – – – – – – – – – – 0 – –
Nucleus – – – – – – – – – – – – 0 – –
Vacuole – – – – – – – – – – – – 2.9 – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.827 1.3975 1.3158 1.1519 1.0744 1.5249 0.3254 0.7618 0.3652 0.4563 0.3529 0.563 -1.1387 -0.1651 -0.7404 -1.0203 -0.5062 -0.6767
Actin 0.0035 0.0014 0.0147 0.0226 0.0074 0.0164 0.0122 0.0123 0.0184 0.0058 0.0341 0.0052 0.008 0.0051 0.0212 0.0119 0.0472 0.0233
Bud 0 0.0001 0.0001 0.0014 0.0013 0.0001 0.0002 0.0006 0.0002 0.0021 0.0004 0 0.0001 0.0001 0.0014 0.0001 0.0004 0.0003
Bud Neck 0.0005 0.0015 0.0019 0.1155 0.024 0.0278 0.0028 0.003 0.0051 0.05 0.0014 0.0332 0.0058 0.0076 0.0107 0.0332 0.001 0.0193
Bud Periphery 0 0.0001 0.0002 0.0013 0.0025 0.0001 0.0003 0.0008 0.0003 0.0056 0.0005 0.0001 0.0001 0.0001 0.0052 0.0001 0.0007 0.0009
Bud Site 0.0005 0.0272 0.0036 0.0094 0.0038 0.0008 0.002 0.0448 0.0065 0.0041 0.0013 0.0004 0.0025 0.0211 0.0076 0.0076 0.0011 0.0011
Cell Periphery 0.0002 0.0003 0.0002 0.0002 0.0005 0.0003 0.0003 0.0011 0.0003 0.0004 0.0003 0.0002 0.0001 0.0002 0.0003 0.0001 0.0001 0.0002
Cytoplasm 0.0717 0.0723 0.0569 0.0788 0.0365 0.1027 0.0948 0.1192 0.106 0.1601 0.1057 0.1559 0.0361 0.0759 0.0517 0.0991 0.0404 0.0388
Cytoplasmic Foci 0.408 0.51 0.4656 0.5104 0.3792 0.4415 0.3899 0.3979 0.5287 0.4149 0.2568 0.4518 0.4719 0.4802 0.4902 0.5858 0.5003 0.5345
Eisosomes 0.0004 0.0001 0.0006 0.0001 0.0007 0.0002 0.0004 0.0003 0.0002 0.0001 0.0005 0.0001 0.0003 0.0001 0.0004 0 0.0006 0.0002
Endoplasmic Reticulum 0.0051 0.0055 0.0087 0.0018 0.0013 0.0044 0.0065 0.0037 0.0052 0.0029 0.0049 0.0059 0.0062 0.0067 0.0072 0.0032 0.0104 0.0034
Endosome 0.0166 0.0326 0.0365 0.0289 0.0274 0.0292 0.1123 0.0481 0.0557 0.0499 0.1207 0.033 0.0324 0.0206 0.0238 0.0304 0.0526 0.0322
Golgi 0.0036 0.0065 0.006 0.0138 0.0085 0.0067 0.0238 0.0079 0.0104 0.0089 0.0324 0.006 0.0068 0.0049 0.0099 0.0084 0.0152 0.0118
Lipid Particles 0.0972 0.1091 0.065 0.0588 0.0341 0.0832 0.062 0.0621 0.067 0.0445 0.0677 0.0662 0.13 0.0972 0.111 0.0895 0.1267 0.0971
Mitochondria 0.0003 0.0006 0.0015 0.0041 0.003 0.0082 0.0139 0.0008 0.0014 0.0123 0.0095 0.0005 0.0006 0.0005 0.0036 0.0006 0.0061 0.0075
None 0.2915 0.1258 0.2332 0.0487 0.4003 0.1807 0.1959 0.175 0.1018 0.1025 0.1883 0.1313 0.1395 0.0575 0.1064 0.0413 0.0364 0.0514
Nuclear Periphery 0.0019 0.0009 0.0028 0.0022 0.0004 0.0005 0.0037 0.0009 0.0009 0.0016 0.001 0.001 0.0032 0.0023 0.002 0.0008 0.0073 0.0019
Nucleolus 0.0013 0.0007 0.0005 0.0007 0.0006 0.0005 0.0005 0.0035 0.0021 0.0005 0.0006 0.0009 0.0004 0.001 0.0005 0.0002 0.0005 0.0009
Nucleus 0.0013 0.0006 0.0007 0.0023 0.0004 0.0007 0.001 0.006 0.0015 0.0023 0.0009 0.0012 0.001 0.0014 0.0012 0.0005 0.0013 0.0011
Peroxisomes 0.0361 0.0683 0.0588 0.0653 0.0458 0.0729 0.0371 0.0705 0.0569 0.0858 0.0769 0.0615 0.0699 0.0949 0.0727 0.0551 0.0775 0.0995
Punctate Nuclear 0.0591 0.0335 0.0396 0.0319 0.0217 0.0216 0.035 0.0371 0.0289 0.0393 0.093 0.0437 0.0839 0.1152 0.072 0.0314 0.0724 0.073
Vacuole 0.0009 0.0026 0.0025 0.0016 0.0006 0.0015 0.0034 0.0042 0.0022 0.0046 0.0023 0.0016 0.0009 0.0072 0.001 0.0005 0.001 0.0012
Vacuole Periphery 0.0001 0.0002 0.0004 0.0004 0.0001 0.0002 0.0018 0.0002 0.0003 0.0018 0.0008 0.0001 0.0003 0.0003 0.0002 0.0001 0.0008 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.8168 8.0911 7.6414 11.8277 5.8675 16.4473 9.4824 14.3058 15.1971 12.8611
Translational Efficiency 0.4166 0.5116 0.5264 0.4824 0.5652 0.2748 0.7737 0.3969 0.5192 0.4163

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Probable catalytic subunit of Nem1p-Spo7p phosphatase holoenzyme; regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology and sporulation; homolog of the human protein Dullard
Localization
Cell Percentages ER (11%)
Cell Cycle Regulation No
Subcompartmental Group ER-2

Nem1

Nem1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Nem1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available