Standard name | |
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Human Ortholog | |
Description | Subunit of the G protein involved in pheromone response; GTP-binding alpha subunit of the heterotrimeric G protein; negatively regulates the mating pathway by sequestering G(beta)gamma and by triggering an adaptive response; activates Vps34p at the endosome; protein abundance increases in response to DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | – | – | 57 | 52 | 64 | 156 | 145 | 89 | – | 87 | 84 | 82 | – | 8 | 7 | – | – | – | – | 0 | 9 | 0 | 1 | 1 |
Bud | – | – | 23 | 12 | 12 | 32 | 16 | 25 | – | 35 | 46 | 13 | – | 1 | 1 | – | – | – | – | 0 | 3 | 0 | 1 | 1 |
Bud Neck | – | – | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | – | 0 | 0 | – | – | – | – | 0 | 8 | 3 | 0 | 0 |
Bud Site | – | – | 0 | 0 | 2 | 5 | 2 | 0 | – | 2 | 0 | 0 | – | 0 | 0 | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | 180 | 169 | 88 | 195 | 201 | 100 | – | 116 | 102 | 115 | – | 26 | 28 | – | – | – | – | 0 | 289 | 39 | 78 | 76 |
Cytoplasm | – | – | 31 | 9 | 17 | 41 | 34 | 61 | – | 63 | 69 | 41 | – | 2 | 8 | – | – | – | – | 1 | 2 | 1 | 1 | 0 |
Endoplasmic Reticulum | – | – | 9 | 15 | 8 | 25 | 26 | 27 | – | 29 | 14 | 27 | – | 0 | 1 | – | – | – | – | 0 | 10 | 0 | 2 | 1 |
Endosome | – | – | 0 | 0 | 1 | 1 | 2 | 2 | – | 2 | 0 | 0 | – | 0 | 0 | – | – | – | – | 0 | 0 | 0 | 0 | 0 |
Golgi | – | – | 0 | 0 | 0 | 9 | 1 | 2 | – | 1 | 0 | 1 | – | 0 | 0 | – | – | – | – | 0 | 0 | 0 | 0 | 0 |
Mitochondria | – | – | 13 | 21 | 5 | 59 | 68 | 49 | – | 88 | 86 | 74 | – | 0 | 0 | – | – | – | – | 1 | 6 | 4 | 2 | 2 |
Nucleus | – | – | 1 | 0 | 0 | 5 | 1 | 0 | – | 1 | 1 | 1 | – | 0 | 0 | – | – | – | – | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | – | – | 2 | 2 | 2 | 3 | 10 | 9 | – | 9 | 2 | 8 | – | 0 | 0 | – | – | – | – | 0 | 0 | 0 | 0 | 0 |
Nucleolus | – | – | 0 | 0 | 0 | 1 | 0 | 5 | – | 0 | 1 | 0 | – | 0 | 0 | – | – | – | – | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | – | – | 3 | 1 | 2 | 2 | 2 | 1 | – | 3 | 0 | 2 | – | 0 | 0 | – | – | – | – | 0 | 0 | 0 | 0 | 0 |
SpindlePole | – | – | 0 | 0 | 1 | 6 | 0 | 1 | – | 0 | 0 | 1 | – | 0 | 0 | – | – | – | – | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | – | – | 2 | 0 | 2 | 9 | 0 | 5 | – | 0 | 0 | 3 | – | 0 | 0 | – | – | – | – | 0 | 3 | 1 | 0 | 0 |
Unique Cell Count | 253 | 218 | 151 | 348 | 319 | 265 | 275 | 261 | 244 | 27 | 32 | 4 | 338 | 53 | 89 | 83 | ||||||||
Labelled Cell Count | 321 | 281 | 204 | 549 | 508 | 376 | 436 | 405 | 368 | 37 | 45 | 4 | 338 | 53 | 89 | 83 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
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Mean Cell GFP Intensity (1e-4) | – | – | 13.2 | 13.7 | 12.2 | 11.1 | 10.9 | 10.7 | – | 9.9 | 9.6 | 10.3 | – | 13.4 | 13.4 | – | – | – | 18.8 | 19.6 | 19.8 |
Std Deviation (1e-4) | – | – | 2.9 | 2.9 | 2.5 | 2.4 | 2.3 | 2.4 | – | 2.4 | 2.3 | 2.6 | – | 2.3 | 2.2 | – | – | – | 3.0 | 3.1 | 3.2 |
Intensity Change (Log2) | – | – | – | 0.06 | -0.11 | -0.24 | -0.27 | -0.29 | – | -0.42 | -0.45 | -0.35 | – | 0.02 | 0.02 | – | – | – | 0.51 | 0.57 | 0.59 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
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Cortical Patches | 0.3 | 4.2 | 5.6 | 5.7 | 2.8 | – | 2.3 | 2.5 | 2.8 | – | 0.8 | -0.1 | – | – | – |
Bud | -1.5 | -0.4 | 0 | -1.9 | 0.1 | – | 1.3 | 2.8 | -1.6 | – | 0 | 0 | – | – | – |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | – | 0 | 0 | – | – | – |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | – | 0 | 0 | – | – | – |
Cell Periphery | 1.6 | -2.6 | -3.8 | -2.0 | -7.6 | – | -6.7 | -7.3 | -5.4 | – | 2.8 | 2.0 | – | – | – |
Cytoplasm | -3.2 | -0.3 | -0.2 | -0.6 | 3.2 | – | 3.2 | 4.1 | 1.4 | – | 0 | 0 | – | – | – |
Endoplasmic Reticulum | 1.6 | 0.8 | 1.9 | 2.3 | 3.0 | – | 3.1 | 1.0 | 3.2 | – | 0 | 0 | – | – | – |
Endosome | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | – | 0 | 0 | – | – | – |
Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | – | 0 | 0 | – | – | – |
Mitochondria | 1.9 | -0.9 | 4.4 | 5.5 | 4.7 | – | 7.8 | 8.0 | 7.4 | – | 0 | 0 | – | – | – |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | – | 0 | 0 | – | – | – |
Nuclear Periphery | 0 | 0 | 0 | 1.9 | 2.1 | – | 2.0 | 0 | 0 | – | 0 | 0 | – | – | – |
Nucleolus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | – | 0 | 0 | – | – | – |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | – | 0 | 0 | – | – | – |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | – | 0 | 0 | – | – | – |
Vacuole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | – | 0 | 0 | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasmic Foci | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Eisosomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Lipid Particles | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
None | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Punctate Nuclear | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | |||||||||
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G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 15.5519 | 15.9878 | 50.0335 | 77.9636 | 35.7905 | 18.6519 | 21.0851 | 31.7826 | 54.8469 | 33.0726 |
Translational Efficiency | 0.8165 | 0.9154 | 1.0914 | 1.0807 | 1.01 | 1.0056 | 0.9288 | 0.8761 | 1.0757 | 1.0119 |
Dataset | Hit |
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Protein Concentration | – |
Protein Localization | – |
Gene Expression | ✔ |
Translational Efficiency | ✔ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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