Standard name
Human Ortholog
Description Subunit of the G protein involved in pheromone response; GTP-binding alpha subunit of the heterotrimeric G protein; negatively regulates the mating pathway by sequestering G(beta)gamma and by triggering an adaptive response; activates Vps34p at the endosome; protein abundance increases in response to DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.23 0.24 0.42 0.45 0.45 0.34 0.32 0.32 0.34 0.3 0.22 0 0 0 0 0
Bud 0.09 0.06 0.08 0.09 0.05 0.09 0.13 0.18 0.05 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.71 0.78 0.58 0.56 0.63 0.38 0.42 0.39 0.47 0.96 0.88 0 0.86 0.75 0.88 0.92
Cytoplasm 0.12 0 0.11 0.12 0.11 0.23 0.23 0.26 0.17 0.07 0.25 0.27 0 0 0 0
Endoplasmic Reticulum 0 0.07 0.05 0.07 0.08 0.1 0.11 0.05 0.11 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0.16 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0.1 0 0.17 0.21 0.18 0.32 0.33 0.3 0 0 0.31 0 0.08 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0.19 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 57 52 64 156 145 89 87 84 82 8 7 0 9 0 1 1
Bud 23 12 12 32 16 25 35 46 13 1 1 0 3 0 1 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 8 3 0 0
Bud Site 0 0 2 5 2 0 2 0 0 0 0
Cell Periphery 180 169 88 195 201 100 116 102 115 26 28 0 289 39 78 76
Cytoplasm 31 9 17 41 34 61 63 69 41 2 8 1 2 1 1 0
Endoplasmic Reticulum 9 15 8 25 26 27 29 14 27 0 1 0 10 0 2 1
Endosome 0 0 1 1 2 2 2 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 9 1 2 1 0 1 0 0 0 0 0 0 0
Mitochondria 13 21 5 59 68 49 88 86 74 0 0 1 6 4 2 2
Nucleus 1 0 0 5 1 0 1 1 1 0 0 0 0 0 0 0
Nuclear Periphery 2 2 2 3 10 9 9 2 8 0 0 0 0 0 0 0
Nucleolus 0 0 0 1 0 5 0 1 0 0 0 0 0 0 0 0
Peroxisomes 3 1 2 2 2 1 3 0 2 0 0 0 0 0 0 0
SpindlePole 0 0 1 6 0 1 0 0 1 0 0 0 0 0 0 0
Vac/Vac Membrane 2 0 2 9 0 5 0 0 3 0 0 0 3 1 0 0
Unique Cell Count 253 218 151 348 319 265 275 261 244 27 32 4 338 53 89 83
Labelled Cell Count 321 281 204 549 508 376 436 405 368 37 45 4 338 53 89 83


Cell Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 13.2 13.7 12.2 11.1 10.9 10.7 9.9 9.6 10.3 13.4 13.4 18.8 19.6 19.8
Std Deviation (1e-4) 2.9 2.9 2.5 2.4 2.3 2.4 2.4 2.3 2.6 2.3 2.2 3.0 3.1 3.2
Intensity Change (Log2) 0.06 -0.11 -0.24 -0.27 -0.29 -0.42 -0.45 -0.35 0.02 0.02 0.51 0.57 0.59


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0.3 4.2 5.6 5.7 2.8 2.3 2.5 2.8 0.8 -0.1
Bud -1.5 -0.4 0 -1.9 0.1 1.3 2.8 -1.6 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1.6 -2.6 -3.8 -2.0 -7.6 -6.7 -7.3 -5.4 2.8 2.0
Cytoplasm -3.2 -0.3 -0.2 -0.6 3.2 3.2 4.1 1.4 0 0
Endoplasmic Reticulum 1.6 0.8 1.9 2.3 3.0 3.1 1.0 3.2 0 0
Endosome 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0
Mitochondria 1.9 -0.9 4.4 5.5 4.7 7.8 8.0 7.4 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 1.9 2.1 2.0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration
Actin
Bud
Bud Neck
Bud Periphery
Bud Site
Cell Periphery
Cytoplasm
Cytoplasmic Foci
Eisosomes
Endoplasmic Reticulum
Endosome
Golgi
Lipid Particles
Mitochondria
None
Nuclear Periphery
Nucleolus
Nucleus
Peroxisomes
Punctate Nuclear
Vacuole
Vacuole Periphery

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.5519 15.9878 50.0335 77.9636 35.7905 18.6519 21.0851 31.7826 54.8469 33.0726
Translational Efficiency 0.8165 0.9154 1.0914 1.0807 1.01 1.0056 0.9288 0.8761 1.0757 1.0119

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

No result is found for the queried gene(s). Please try another query.


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gpa1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available