Standard name
Human Ortholog
Description Mitochondrial manganese superoxide dismutase; protects cells against oxygen toxicity and oxidative stress; human mitochondrial SOD2 can complement a yeast null mutant and human cytoplasmic SOD1 can also complement when targeted to the mitochondrial matrix
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0.05 0.05 0.08
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.32 0.18 0.15 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.06 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0.14 0.16 0.29 0 0 – 0 0.05 0 0 0 0 0.12 0.26 0.37 0.18 0 0.11 0.05 0.09 0.08 0.1
Mitochondria 0.88 0.88 0.78 0.87 0.74 0.89 0.74 – 0.9 0.86 0.87 0.85 0.82 0.82 0.77 0.11 0.25 0.21 0.78 0.72 0.83 0.63 0.64 0.59
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.12 0.07 0.07 0 0.09 0.17 0.16 – 0.13 0.14 0.15 0.14 0.05 0.06 0 0.15 0.1 0.31 0 0 0 0 0 0
Peroxisomes 0 0.06 0.13 0.07 0 0 0 – 0 0 0 0 0.19 0.14 0.16 0 0.06 0 0.06 0 0.05 0.12 0.11 0.1
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.14 0.07 0 0.08 0.05 0.09 – 0 0 0.05 0 0.05 0 0 0.15 0.13 0.1 0 0.05 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 2 4 5 3 0 0 – 6 5 4 3 1 2 4 2 5 0 7 6 4 10 15 23
Bud 0 5 2 6 3 3 1 – 3 5 1 4 4 0 4 1 2 0 0 1 0 3 7 1
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 1 0 0 0 0 0 0 0 0 1 2 1
Bud Site 0 0 0 0 1 0 0 – 0 1 0 1 0 1 0 1 0 0 – – – – – –
Cell Periphery 0 4 4 2 1 1 2 – 0 0 0 0 1 4 3 2 2 1 0 0 0 0 0 1
Cytoplasm 4 1 1 3 7 1 10 – 8 14 9 9 4 9 6 17 12 6 0 1 1 0 0 0
Endoplasmic Reticulum 2 0 0 1 0 1 0 – 2 2 1 0 1 0 3 3 2 2 0 1 0 0 0 2
Endosome 1 1 10 7 6 0 5 – 0 1 1 1 4 4 5 1 0 0 7 8 8 4 4 8
Golgi 9 10 38 33 72 2 12 – 9 26 16 13 6 8 39 14 25 7 8 34 16 18 25 28
Mitochondria 197 250 213 176 184 164 204 – 414 486 316 364 193 224 240 6 17 8 190 219 273 125 206 172
Nucleus 0 0 0 0 1 1 1 – 2 0 0 0 0 1 0 0 0 3 0 0 0 0 0 0
Nuclear Periphery 8 7 4 3 6 6 10 – 8 5 7 17 2 4 8 0 0 0 4 1 1 1 4 5
Nucleolus 26 19 20 9 22 32 44 – 58 78 56 60 11 16 13 8 7 12 3 2 0 2 5 2
Peroxisomes 8 17 35 14 9 0 6 – 0 3 1 5 45 37 51 0 4 0 13 7 15 22 34 30
SpindlePole 3 1 11 0 2 0 3 – 5 3 2 4 2 5 6 2 0 0 4 2 2 3 5 6
Vac/Vac Membrane 7 40 20 8 21 10 24 – 17 19 17 19 12 12 10 8 9 4 2 16 1 1 4 4
Unique Cell Count 224 284 273 203 249 185 274 462 566 365 426 235 274 312 53 67 39 244 306 329 199 321 292
Labelled Cell Count 265 357 362 267 338 221 322 532 648 431 500 287 327 392 65 85 43 244 306 329 199 321 292


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 15.9 15.6 14.6 16.2 19.7 15.5 22.7 – 25.5 27.2 28.6 28.2 19.0 20.2 21.5 36.4 36.8 39.1 15.7 18.7 16.7
Std Deviation (1e-4) 6.5 5.0 3.5 7.4 5.5 5.4 7.5 – 9.0 10.9 12.2 12.4 6.0 5.8 6.8 19.0 18.1 17.9 11.9 18.6 12.6
Intensity Change (Log2) – – – 0.15 0.43 0.09 0.64 – 0.8 0.9 0.97 0.95 0.38 0.47 0.56 1.32 1.33 1.42 0.1 0.36 0.19


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 2.7 – 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome -0.1 -0.8 0 -1.3 – 0 0 0 0 -1.3 -1.6 -1.6 0 0 0
Golgi 0.7 4.2 -4.8 -3.9 – -6.4 -4.8 -4.3 -5.4 -4.5 -4.6 -0.5 2.3 4.4 0.7
Mitochondria 2.4 -1.1 2.9 -1.0 – 4.3 2.9 2.8 2.5 1.2 1.1 -0.3 -9.5 -8.3 -7.4
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 1.6 – 0 0 0 1.9 0 0 0.9 0 0 0
Nucleolus -1.3 0.6 3.3 3.2 – 2.2 2.7 3.1 2.7 -1.2 -0.7 -1.7 0 0.8 0
Peroxisomes -2.1 -3.8 -5.1 -4.7 – -7.9 -8.0 -6.8 -6.5 2.0 0.2 1.2 -2.8 -1.6 0
SpindlePole 0 -2.4 0 -2.2 – -2.6 0 -3.1 -2.8 -2.3 -1.5 -1.5 0 0 0
Vacuole -1.6 0.5 -0.8 0.6 – -2.2 -2.6 -1.4 -1.6 -1.0 -1.5 -2.3 0 1.6 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 26.5956 29.2165 27.8986 29.3766 14.9889 26.8137 30.8832 31.9919 32.8686 27.2843 27.4177 30.3983 39.2135 37.2657 37.2518 33.3173 32.862 37.9649
Actin 0.019 0.0013 0.0136 0.0003 0.02 0.0014 0.0173 0.0166 0.0037 0.0055 0.0363 0.0006 0.0095 0.0003 0.0075 0.0099 0.0052 0.0041
Bud 0.0003 0.0001 0.0001 0.0002 0.0014 0 0.0005 0.0002 0.0001 0.0001 0.0001 0 0.0001 0 0 0.0002 0.0001 0
Bud Neck 0.0029 0 0.0001 0.0009 0.0008 0 0.0002 0.0001 0.0001 0 0 0.0001 0.0002 0 0.0003 0.0001 0.0002 0.0001
Bud Periphery 0.0005 0.0001 0.0002 0.0001 0.0031 0.0001 0.0013 0.0003 0.0001 0.0001 0.0001 0 0.0001 0.0001 0 0.0009 0.0001 0.0001
Bud Site 0.0016 0.0002 0.0007 0.0093 0.0031 0 0.0009 0.0013 0.0002 0.0001 0.0001 0 0.0004 0.0001 0.0001 0.0002 0.0005 0
Cell Periphery 0.0003 0.0002 0.0001 0.0001 0.0004 0.0001 0.0002 0.001 0.0016 0 0.0001 0.0001 0.0002 0.0003 0.0001 0.0002 0.0001 0.0001
Cytoplasm 0.0039 0 0.0009 0.0003 0.0025 0.0005 0.0004 0 0.0004 0 0 0 0.0019 0 0.0001 0.0001 0.0002 0
Cytoplasmic Foci 0.0148 0.0015 0.0016 0.0243 0.0259 0.0094 0.0018 0.0008 0.0004 0.0004 0.0003 0.0001 0.0061 0.0001 0.0005 0.0024 0.0091 0.0001
Eisosomes 0.0005 0.0004 0.001 0.0002 0.0004 0.0002 0.001 0.0045 0.002 0.001 0.002 0.0006 0.0007 0.0004 0.0018 0.0003 0.0002 0.0003
Endoplasmic Reticulum 0.0011 0 0.0002 0.0001 0.0082 0.0001 0.0018 0 0.0006 0 0 0 0.0007 0 0 0.0003 0.0001 0.0001
Endosome 0.0095 0.0005 0.0019 0.0011 0.062 0.0003 0.0053 0.0009 0.0032 0 0.0001 0.0001 0.0015 0.0001 0.0003 0.0046 0.0026 0.0007
Golgi 0.004 0.0012 0.002 0.0007 0.0199 0.0009 0.0022 0.0056 0.0009 0.0002 0.0016 0.0002 0.0007 0.0002 0.0013 0.0028 0.003 0.0024
Lipid Particles 0.0244 0.0065 0.0048 0.0039 0.0415 0.0051 0.0116 0.0133 0.0046 0.0082 0.0029 0.0035 0.0033 0.0047 0.0039 0.0089 0.0121 0.0025
Mitochondria 0.8781 0.9707 0.9594 0.9522 0.7036 0.9702 0.8945 0.91 0.9548 0.9715 0.9281 0.9883 0.9479 0.9786 0.9675 0.9505 0.9386 0.983
None 0.0032 0 0.0011 0.0001 0.0044 0.001 0.0019 0 0.0004 0 0 0 0.0025 0 0.0005 0.0007 0.0004 0
Nuclear Periphery 0.0069 0 0.0006 0 0.0337 0.0002 0.0191 0.0001 0.0049 0.0001 0 0 0.0009 0.0001 0.0001 0.0028 0.0004 0.0002
Nucleolus 0.0066 0.0008 0.0003 0.0009 0.0035 0.0004 0.0069 0.0018 0.0004 0.0001 0 0.0004 0.0059 0.0006 0.0004 0.0003 0.0009 0
Nucleus 0.0037 0.0001 0.0003 0.0003 0.0128 0.0001 0.0031 0.0004 0.0018 0 0 0 0.0018 0.0001 0.0001 0.0003 0.0002 0
Peroxisomes 0.0053 0.0064 0.0046 0.0022 0.0276 0.0039 0.0224 0.0058 0.0024 0.0109 0.028 0.003 0.0029 0.0018 0.0018 0.0047 0.0128 0.0003
Punctate Nuclear 0.0073 0 0.0029 0.0022 0.0058 0.0002 0.0005 0 0.0005 0 0 0 0.0053 0 0.0002 0.0054 0.0093 0
Vacuole 0.0017 0.0028 0.0011 0.0004 0.0087 0.0003 0.0023 0.0059 0.0027 0.0001 0.0001 0.0004 0.0005 0.0013 0.0009 0.0006 0.0004 0.0004
Vacuole Periphery 0.0044 0.0072 0.0026 0.0002 0.0108 0.0057 0.0047 0.0313 0.0142 0.0017 0.0002 0.0025 0.007 0.0111 0.0126 0.004 0.0034 0.0056

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 149.3211 157.3706 141.7991 153.2861 158.9569 157.0931 126.4414 124.653 151.4079 118.4113
Translational Efficiency 1.4431 1.3488 1.5608 1.6581 1.7461 1.6634 1.5989 2.3388 1.9496 1.8696

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✔
Standard name
Human Ortholog
Description Mitochondrial manganese superoxide dismutase; protects cells against oxygen toxicity and oxidative stress; human mitochondrial SOD2 can complement a yeast null mutant and human cytoplasmic SOD1 can also complement when targeted to the mitochondrial matrix
Localization
Cell Percentages mitochondrion (99%)
Cell Cycle Regulation No
Subcompartmental Group mito-3

Sod2

Sod2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sod2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available