Standard name | |
---|---|
Human Ortholog | |
Description | Alpha subunit of the mitochondrial processing protease (MPP); essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 1 | 2 | 5 | 0 | 1 | 1 | 1 | 2 | 0 | 2 | 1 | 1 | 4 | 2 | 1 | 2 | 3 | 7 | 4 | 5 | 2 | 14 | 37 |
Bud | 2 | 0 | 0 | 1 | 6 | 6 | 7 | 8 | 8 | 8 | 4 | 9 | 1 | 0 | 3 | 2 | 0 | 2 | 1 | 0 | 0 | 0 | 1 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 2 | 0 | 2 | 3 | 2 | 2 | 1 | 1 | 2 | 1 | 0 | 1 | 3 | 3 | 0 | 4 | 3 | 0 | 1 | 0 | 0 | 0 | 0 |
Cytoplasm | 2 | 0 | 1 | 7 | 12 | 11 | 13 | 15 | 8 | 4 | 5 | 8 | 0 | 6 | 4 | 5 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 2 | 2 | 0 | 1 | 1 | 0 | 1 | 3 | 0 | 1 | 1 | 0 | 1 | 0 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Endosome | 0 | 0 | 0 | 0 | 6 | 2 | 2 | 3 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 5 | 2 |
Golgi | 1 | 0 | 2 | 6 | 4 | 1 | 1 | 0 | 1 | 0 | 0 | 2 | 0 | 9 | 16 | 4 | 19 | 22 | 11 | 10 | 15 | 5 | 9 | 31 |
Mitochondria | 241 | 96 | 171 | 189 | 242 | 257 | 359 | 406 | 275 | 364 | 301 | 312 | 64 | 145 | 217 | 6 | 7 | 6 | 228 | 77 | 144 | 70 | 149 | 203 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 2 | 1 | 1 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 7 | 4 | 8 | 5 | 7 | 13 | 25 | 31 | 36 | 29 | 22 | 24 | 3 | 5 | 7 | 2 | 1 | 4 | 1 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 22 | 6 | 9 | 14 | 30 | 40 | 51 | 67 | 56 | 41 | 30 | 37 | 4 | 7 | 7 | 6 | 6 | 5 | 1 | 2 | 0 | 0 | 1 | 1 |
Peroxisomes | 4 | 0 | 2 | 6 | 2 | 4 | 10 | 11 | 2 | 7 | 3 | 2 | 1 | 4 | 7 | 9 | 3 | 2 | 8 | 1 | 11 | 6 | 23 | 29 |
SpindlePole | 0 | 0 | 0 | 1 | 2 | 2 | 3 | 6 | 0 | 5 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 |
Vac/Vac Membrane | 16 | 7 | 7 | 5 | 8 | 1 | 12 | 11 | 8 | 8 | 6 | 9 | 3 | 11 | 14 | 5 | 4 | 8 | 1 | 3 | 2 | 0 | 1 | 6 |
Unique Cell Count | 258 | 100 | 180 | 201 | 273 | 281 | 407 | 479 | 297 | 386 | 321 | 338 | 65 | 163 | 236 | 36 | 42 | 52 | 262 | 104 | 184 | 88 | 212 | 326 |
Labelled Cell Count | 298 | 118 | 204 | 241 | 323 | 342 | 489 | 564 | 402 | 472 | 376 | 412 | 78 | 195 | 281 | 42 | 49 | 59 | 262 | 104 | 184 | 88 | 212 | 326 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 16.9 | 16.3 | 15.0 | 17.7 | 17.1 | 14.4 | 14.0 | 12.9 | 12.6 | 11.9 | 11.3 | 12.4 | 18.8 | 19.5 | 18.2 | 16.1 | 18.6 | 20.7 | 17.5 | 17.3 | 18.5 |
Std Deviation (1e-4) | 4.4 | 3.9 | 4.4 | 5.1 | 4.5 | 4.1 | 3.8 | 3.9 | 3.7 | 3.5 | 3.0 | 3.7 | 4.3 | 9.5 | 4.0 | 4.7 | 6.2 | 5.6 | 5.0 | 4.6 | 4.8 |
Intensity Change (Log2) | – | – | – | 0.24 | 0.19 | -0.06 | -0.1 | -0.22 | -0.25 | -0.33 | -0.41 | -0.27 | 0.32 | 0.38 | 0.28 | 0.1 | 0.31 | 0.46 | 0.22 | 0.21 | 0.3 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 2.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.3 | 2.8 | 0 | 0 | 8.6 |
Mitochondria | -0.4 | -2.3 | -1.4 | -2.6 | -3.5 | -1.0 | -0.3 | -0.6 | -1.2 | 1.2 | -2.1 | -1.2 | -11.2 | -11.5 | -12.5 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | -1.1 | -1.1 | 0.1 | 0.8 | 1.0 | 2.8 | 1.4 | 1.1 | 1.2 | 0 | -0.7 | -0.8 | 0 | 0 | 0 |
Nucleolus | 0.8 | 2.2 | 3.1 | 2.8 | 3.2 | 4.3 | 2.2 | 1.7 | 2.3 | 0 | -0.3 | -1.1 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -0.8 | -0.6 | 0 | -0.6 | 0 | -0.7 | 0 | 0 | -0.8 | 0 | 1.2 | 0.9 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 25.1233 | 26.6518 | 25.9525 | 24.7636 | 23.2103 | 26.1643 | 23.641 | 26.0834 | 23.0875 | 23.736 | 21.6066 | 23.6813 | 27.3441 | 30.1366 | 28.1674 | 24.9279 | 22.2337 | 26.479 |
Actin | 0.0112 | 0.0005 | 0.003 | 0.0001 | 0.0145 | 0.0106 | 0.0335 | 0.0013 | 0.0023 | 0.0005 | 0.0087 | 0.0002 | 0.0095 | 0.0056 | 0.009 | 0.0007 | 0.0229 | 0.0004 |
Bud | 0.0002 | 0 | 0.0001 | 0 | 0.0005 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0.0006 | 0 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0002 | 0 |
Bud Neck | 0.0001 | 0 | 0.0001 | 0 | 0.0027 | 0.0001 | 0.001 | 0 | 0 | 0 | 0.0004 | 0 | 0.0015 | 0.0002 | 0.0001 | 0 | 0.0001 | 0 |
Bud Periphery | 0.0003 | 0 | 0.0002 | 0 | 0.0006 | 0.0001 | 0.0002 | 0.0001 | 0.0004 | 0 | 0.0007 | 0 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0 |
Bud Site | 0.0003 | 0 | 0.0004 | 0 | 0.0073 | 0.0001 | 0.001 | 0.0001 | 0.0013 | 0 | 0.005 | 0 | 0.003 | 0.0003 | 0.0025 | 0.0003 | 0.0005 | 0 |
Cell Periphery | 0.0002 | 0.0001 | 0.0003 | 0 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0003 | 0.0001 |
Cytoplasm | 0.0005 | 0 | 0.0001 | 0 | 0.0004 | 0 | 0.0001 | 0 | 0 | 0 | 0.0008 | 0 | 0.0046 | 0 | 0.0005 | 0.0004 | 0.0009 | 0 |
Cytoplasmic Foci | 0.0007 | 0.0001 | 0.0007 | 0 | 0.0013 | 0.0016 | 0.0069 | 0.0002 | 0.0001 | 0 | 0.047 | 0.0001 | 0.0188 | 0.0005 | 0.0063 | 0.0198 | 0.0317 | 0.0002 |
Eisosomes | 0.0007 | 0.0002 | 0.0008 | 0.0001 | 0.0002 | 0.0002 | 0.0003 | 0.0003 | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0.0003 | 0.0003 | 0.0002 | 0.0004 | 0.0008 | 0.0001 |
Endoplasmic Reticulum | 0.0004 | 0 | 0.0004 | 0 | 0.0005 | 0.0001 | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0.0012 | 0 | 0.0001 | 0.0001 | 0.0007 | 0 |
Endosome | 0.0049 | 0.0005 | 0.0005 | 0 | 0.0035 | 0.0011 | 0.0008 | 0.0007 | 0.0001 | 0.0001 | 0.0032 | 0.0003 | 0.0043 | 0.0004 | 0.0006 | 0.0004 | 0.0096 | 0.0004 |
Golgi | 0.0021 | 0.0016 | 0.0009 | 0.0001 | 0.0027 | 0.0031 | 0.0024 | 0.0036 | 0.0003 | 0.0001 | 0.0062 | 0.0009 | 0.0025 | 0.01 | 0.0045 | 0.0005 | 0.006 | 0.0055 |
Lipid Particles | 0.0054 | 0.0038 | 0.0033 | 0.0015 | 0.0024 | 0.0062 | 0.0113 | 0.0054 | 0.0008 | 0.0022 | 0.0551 | 0.0113 | 0.0229 | 0.0044 | 0.0094 | 0.0283 | 0.0459 | 0.0124 |
Mitochondria | 0.947 | 0.983 | 0.9758 | 0.9949 | 0.95 | 0.9509 | 0.9009 | 0.9781 | 0.9871 | 0.9955 | 0.8131 | 0.9659 | 0.8964 | 0.9705 | 0.9497 | 0.9227 | 0.8015 | 0.966 |
None | 0.0013 | 0 | 0.0005 | 0 | 0.0003 | 0 | 0.0001 | 0 | 0 | 0 | 0.0038 | 0 | 0.0082 | 0 | 0.0052 | 0.0024 | 0.0079 | 0 |
Nuclear Periphery | 0.0023 | 0 | 0.0002 | 0 | 0.0009 | 0.0005 | 0.0001 | 0 | 0 | 0 | 0.0033 | 0.0004 | 0.0068 | 0 | 0.0004 | 0.0009 | 0.006 | 0 |
Nucleolus | 0.0026 | 0.0001 | 0.0002 | 0.0001 | 0.0004 | 0.0001 | 0.016 | 0.0001 | 0.0001 | 0 | 0.0064 | 0.0001 | 0.0012 | 0.0003 | 0.0011 | 0.0079 | 0.0088 | 0.0001 |
Nucleus | 0.0055 | 0 | 0.0001 | 0 | 0.0008 | 0.0001 | 0.0012 | 0 | 0.0001 | 0 | 0.0014 | 0.0001 | 0.0014 | 0 | 0.0007 | 0.0016 | 0.0012 | 0 |
Peroxisomes | 0.0017 | 0.001 | 0.0051 | 0.0002 | 0.0008 | 0.004 | 0.0196 | 0.0029 | 0.0022 | 0.0006 | 0.0266 | 0.0003 | 0.0048 | 0.0045 | 0.0017 | 0.0034 | 0.0124 | 0.0012 |
Punctate Nuclear | 0.0033 | 0 | 0.0001 | 0 | 0.0021 | 0.0001 | 0.0004 | 0 | 0 | 0 | 0.0121 | 0 | 0.0075 | 0 | 0.0038 | 0.0024 | 0.0066 | 0 |
Vacuole | 0.0018 | 0.0011 | 0.0017 | 0.0002 | 0.0015 | 0.0012 | 0.0004 | 0.0008 | 0.0014 | 0.0001 | 0.0007 | 0.0008 | 0.0011 | 0.0002 | 0.0003 | 0.0008 | 0.0023 | 0.0005 |
Vacuole Periphery | 0.0075 | 0.0077 | 0.0055 | 0.0028 | 0.0061 | 0.0199 | 0.0036 | 0.0064 | 0.003 | 0.0005 | 0.0042 | 0.0195 | 0.0034 | 0.0025 | 0.0038 | 0.0066 | 0.0334 | 0.0131 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 41.8822 | 30.6328 | 44.4515 | 43.2031 | 30.113 | 46.4254 | 48.1799 | 45.7577 | 56.4999 | 51.0109 |
Translational Efficiency | 0.8544 | 1.0443 | 0.7089 | 0.933 | 1.0504 | 1.0822 | 0.8793 | 0.8884 | 0.8619 | 0.8726 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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