Standard name | |
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Human Ortholog | |
Description | DNA helicase involved in rDNA replication and Ty1 transposition; binds to and suppresses DNA damage at G4 motifs in vivo; relieves replication fork pauses at telomeric regions; structurally and functionally related to Pif1p |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 1 | 2 | 0 | 1 | 3 |
Bud | 1 | 0 | 0 | 1 | 0 | 1 | 3 | 2 | 25 | 12 | 8 | 12 | 0 | 0 | 0 | – | – | – | 2 | 7 | 6 | 3 | 6 | 7 |
Bud Neck | 1 | 0 | 36 | 13 | 30 | 24 | 20 | 26 | 15 | 13 | 5 | 31 | 1 | 0 | 0 | – | – | – | 0 | 2 | 4 | 2 | 8 | 3 |
Bud Site | 0 | 1 | 0 | 1 | 0 | 3 | 5 | 1 | 5 | 8 | 6 | 5 | 0 | 0 | 0 | – | – | – | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 1 | 1 | 0 | 0 | 2 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 32 | 37 | 214 | 168 | 267 | 284 | 338 | 325 | 234 | 262 | 282 | 275 | 70 | 138 | 140 | – | – | – | 17 | 26 | 88 | 41 | 97 | 111 |
Endoplasmic Reticulum | 2 | 4 | 5 | 2 | 0 | 1 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 1 | 3 | 0 | 2 | 2 |
Endosome | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 4 | 24 | 5 | – | – | – | 3 | 4 | 5 | 3 | 8 | 10 |
Golgi | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 2 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 24 | 63 | 15 | 26 | 14 | 119 | 144 | 86 | 301 | 274 | 318 | 202 | 51 | 10 | 11 | – | – | – | 3 | 11 | 35 | 14 | 47 | 29 |
Nucleus | 126 | 164 | 172 | 79 | 66 | 65 | 84 | 82 | 80 | 68 | 67 | 54 | 123 | 124 | 108 | – | – | – | 130 | 177 | 218 | 46 | 59 | 25 |
Nuclear Periphery | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 2 | 0 | 0 | 0 |
Nucleolus | 1 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 1 | 3 | 0 | 2 | 0 | 1 | – | – | – | 3 | 2 | 2 | 3 | 7 | 1 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 1 | 0 | 1 | 0 |
SpindlePole | 3 | 2 | 6 | 6 | 6 | 3 | 10 | 13 | 4 | 7 | 9 | 17 | 4 | 1 | 1 | – | – | – | 10 | 10 | 37 | 10 | 20 | 23 |
Vac/Vac Membrane | 33 | 24 | 12 | 6 | 11 | 3 | 14 | 17 | 6 | 8 | 20 | 15 | 47 | 89 | 61 | – | – | – | 53 | 43 | 192 | 77 | 197 | 138 |
Unique Cell Count | 191 | 255 | 438 | 285 | 368 | 442 | 546 | 499 | 531 | 533 | 603 | 513 | 268 | 356 | 307 | 231 | 295 | 626 | 211 | 476 | 369 | |||
Labelled Cell Count | 223 | 297 | 465 | 304 | 394 | 505 | 623 | 554 | 672 | 655 | 719 | 611 | 302 | 386 | 327 | 231 | 295 | 626 | 211 | 476 | 369 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.2 | 4.1 | 4.7 | 4.1 | 4.8 | 3.6 | 3.9 | 4.1 | 3.2 | 3.2 | 3.2 | 3.6 | 4.1 | 4.3 | 4.3 | – | – | – | 4.1 | 3.9 | 4.0 |
Std Deviation (1e-4) | 0.7 | 0.7 | 0.6 | 0.9 | 1.3 | 0.9 | 1.0 | 1.0 | 0.9 | 0.7 | 0.5 | 0.7 | 0.7 | 1.0 | 1.5 | – | – | – | 1.9 | 1.0 | 1.0 |
Intensity Change (Log2) | – | – | – | -0.19 | 0.03 | -0.4 | -0.26 | -0.2 | -0.55 | -0.58 | -0.58 | -0.38 | -0.2 | -0.13 | -0.12 | – | – | – | -0.2 | -0.28 | -0.22 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -1.1308 | -0.8845 | -1.1948 | -1.1928 | -1.2243 | -1.0222 | -0.4735 | -0.1855 | -0.2739 | -0.4252 | -0.2263 | -0.2899 | -0.3426 | -0.3577 | -0.4022 | 0.1096 | 0.0157 | -0.4267 |
Actin | 0.002 | 0.005 | 0.0003 | 0.0003 | 0.0004 | 0.0005 | 0.013 | 0.0001 | 0.0091 | 0.0159 | 0.0064 | 0.0019 | 0.0196 | 0.0072 | 0.0007 | 0.0009 | 0.0084 | 0.0008 |
Bud | 0.0003 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0005 | 0.0022 | 0.0003 | 0.0003 | 0.0017 | 0.0003 | 0.0009 | 0.0007 | 0.0002 | 0.0005 | 0.0008 | 0.0003 |
Bud Neck | 0.0074 | 0.0008 | 0.0009 | 0.0007 | 0.0032 | 0.003 | 0.0017 | 0.0009 | 0.003 | 0.003 | 0.0022 | 0.0078 | 0.0127 | 0.0012 | 0.0009 | 0.0066 | 0.003 | 0.003 |
Bud Periphery | 0.0006 | 0.0002 | 0 | 0 | 0.0001 | 0.0001 | 0.0013 | 0.0006 | 0.0004 | 0.0003 | 0.0047 | 0.0009 | 0.0031 | 0.0011 | 0.0006 | 0.0022 | 0.001 | 0.0013 |
Bud Site | 0.0013 | 0.0029 | 0.0004 | 0.0002 | 0.001 | 0.0003 | 0.0035 | 0.0093 | 0.0021 | 0.0013 | 0.004 | 0.0003 | 0.0094 | 0.0039 | 0.0009 | 0.0051 | 0.0022 | 0.0008 |
Cell Periphery | 0.0002 | 0.0001 | 0 | 0 | 0 | 0 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0.0016 | 0.0002 | 0.0002 |
Cytoplasm | 0.0744 | 0.0902 | 0.0724 | 0.0601 | 0.0891 | 0.0573 | 0.0799 | 0.0928 | 0.0514 | 0.0496 | 0.0753 | 0.0484 | 0.0528 | 0.0457 | 0.0279 | 0.0358 | 0.0562 | 0.045 |
Cytoplasmic Foci | 0.0063 | 0.0055 | 0.0035 | 0.0014 | 0.0058 | 0.0041 | 0.0192 | 0.0017 | 0.005 | 0.012 | 0.0091 | 0.0031 | 0.0137 | 0.0024 | 0.0034 | 0.0032 | 0.0075 | 0.0031 |
Eisosomes | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0002 | 0.0001 | 0.0002 | 0 | 0.0008 | 0 | 0.0001 | 0.0003 | 0.0002 | 0 |
Endoplasmic Reticulum | 0.0075 | 0.0057 | 0.003 | 0.0021 | 0.0085 | 0.0027 | 0.0067 | 0.0032 | 0.0035 | 0.0018 | 0.0059 | 0.003 | 0.0034 | 0.0017 | 0.0009 | 0.0008 | 0.0047 | 0.0012 |
Endosome | 0.0135 | 0.0075 | 0.0039 | 0.0023 | 0.0133 | 0.0049 | 0.027 | 0.0027 | 0.0099 | 0.0049 | 0.0175 | 0.007 | 0.0168 | 0.0025 | 0.0051 | 0.003 | 0.0247 | 0.0025 |
Golgi | 0.0006 | 0.0008 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0034 | 0.0001 | 0.0031 | 0.0015 | 0.0053 | 0.0012 | 0.0042 | 0.0001 | 0.0014 | 0.0007 | 0.0052 | 0.0003 |
Lipid Particles | 0.0023 | 0.003 | 0.0013 | 0.0002 | 0.0025 | 0.0013 | 0.0107 | 0.0002 | 0.0029 | 0.0014 | 0.035 | 0.0018 | 0.0082 | 0.0006 | 0.0014 | 0.0024 | 0.0084 | 0.001 |
Mitochondria | 0.0015 | 0.0016 | 0.0006 | 0.0006 | 0.0009 | 0.0006 | 0.0037 | 0.0006 | 0.0047 | 0.0015 | 0.0288 | 0.0049 | 0.0147 | 0.0007 | 0.0039 | 0.0114 | 0.0052 | 0.0016 |
None | 0.1496 | 0.1857 | 0.1129 | 0.0723 | 0.2379 | 0.1353 | 0.1248 | 0.0737 | 0.0864 | 0.0315 | 0.0836 | 0.0716 | 0.1528 | 0.1371 | 0.1336 | 0.1773 | 0.1158 | 0.0937 |
Nuclear Periphery | 0.0684 | 0.0331 | 0.0498 | 0.0432 | 0.0469 | 0.0387 | 0.0627 | 0.0193 | 0.0293 | 0.0206 | 0.0321 | 0.0162 | 0.0318 | 0.0248 | 0.0268 | 0.0316 | 0.0327 | 0.0084 |
Nucleolus | 0.0158 | 0.0145 | 0.0123 | 0.0289 | 0.0126 | 0.0165 | 0.0104 | 0.0205 | 0.0276 | 0.0132 | 0.0083 | 0.02 | 0.0196 | 0.0222 | 0.0366 | 0.0264 | 0.0156 | 0.0179 |
Nucleus | 0.5778 | 0.5965 | 0.6633 | 0.7219 | 0.5201 | 0.6596 | 0.562 | 0.7505 | 0.6887 | 0.7891 | 0.6059 | 0.7502 | 0.5604 | 0.6776 | 0.7089 | 0.6026 | 0.6495 | 0.7511 |
Peroxisomes | 0.0002 | 0.0004 | 0.0001 | 0.0001 | 0.0004 | 0.0002 | 0.0083 | 0.0001 | 0.0036 | 0.0068 | 0.0061 | 0.0004 | 0.0083 | 0.0001 | 0.0012 | 0.0019 | 0.0029 | 0.001 |
Punctate Nuclear | 0.062 | 0.0402 | 0.0725 | 0.0643 | 0.0536 | 0.0717 | 0.0479 | 0.0167 | 0.0644 | 0.0425 | 0.0626 | 0.0578 | 0.0546 | 0.0679 | 0.043 | 0.0799 | 0.05 | 0.0657 |
Vacuole | 0.0063 | 0.0047 | 0.0018 | 0.001 | 0.0027 | 0.0023 | 0.0095 | 0.0041 | 0.003 | 0.002 | 0.0035 | 0.0022 | 0.0085 | 0.002 | 0.0017 | 0.0047 | 0.0044 | 0.0011 |
Vacuole Periphery | 0.002 | 0.001 | 0.0007 | 0.0005 | 0.0008 | 0.0006 | 0.0034 | 0.0005 | 0.0011 | 0.0005 | 0.0015 | 0.0007 | 0.0035 | 0.0004 | 0.0006 | 0.0012 | 0.0015 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 10.6683 | 14.9864 | 7.7507 | 6.2984 | 10.8679 | 4.0619 | 12.2829 | 9.9171 | 6.9562 | 11.7217 |
Translational Efficiency | 0.7817 | 0.7138 | 0.6178 | 0.6993 | 0.7391 | 1.2107 | 0.5426 | 0.6507 | 0.6161 | 0.6003 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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