Standard name
Human Ortholog
Description Subunit G of the V1 peripheral membrane domain of V-ATPase; part of the electrogenic proton pump found throughout the endomembrane system; involved in vacuolar acidification; the V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase) has eight subunits
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.08 0.06 0.05 0.11 0.12 0.2 0.23 0.36 0.32 0.37 0.41 0.43 0.09 0.06 0.06 0.06 0.11 0.09 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0.07 0.05 0.07 0.06 0.08 0.07 0.05 0 0.05 0 0.05 0.08 0.06 0 0 0 0.05 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0
Golgi 0.15 0.1 0 0.27 0.22 0.05 0.11 0.09 0.07 0.09 0.09 0 0.17 0.23 0.27 0.16 0.18 0.21 0 0 0 0 0 0
Mitochondria 0 0 0.11 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0.05 0
Nuclear Periphery 0 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.18 0.06 0.06 0.06
Nucleolus 0.05 0 0.22 0 0 0 0 0 0 0 0 0 0 0 0 0.13 0.11 0.13 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.83 0.91 0.88 0.82 0.84 0.67 0.67 0.49 0.6 0.54 0.48 0.51 0.88 0.9 0.9 0.81 0.83 0.81 0.79 0.86 0.63 0.77 0.76 0.78
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 1 0 2 3 2 2 0 1 1 1 1 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 1 0 1 1 1 0 1 0 0 0 0 0 0 0 0 0 0 0 3 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 1 0 2 1 1 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 10 14 4 20 23 41 83 128 88 108 88 115 15 15 21 8 8 9 4 8 0 2 3 0
Endoplasmic Reticulum 2 0 0 1 0 14 19 24 17 22 15 13 4 13 6 7 6 6 0 0 1 4 4 3
Endosome 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 3 1 4 0 6 5
Golgi 20 21 3 51 42 11 41 32 19 26 19 11 30 62 89 21 14 21 1 1 3 1 1 1
Mitochondria 0 0 8 3 1 7 7 2 6 2 6 8 0 0 0 5 3 5 0 0 1 1 3 2
Nucleus 2 5 0 0 0 3 1 3 1 2 1 0 0 2 1 1 2 0 7 12 1 1 12 9
Nuclear Periphery 1 0 6 6 2 3 5 15 6 2 1 5 0 2 3 0 0 0 5 4 15 5 15 12
Nucleolus 6 5 16 4 4 6 9 6 4 3 1 0 6 10 9 17 8 13 3 2 2 2 4 9
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
Vac/Vac Membrane 110 196 65 154 160 137 244 178 165 157 104 136 153 243 295 109 63 80 114 202 56 69 188 175
Unique Cell Count 133 216 74 188 190 205 364 360 273 293 217 265 174 271 327 135 76 99 144 234 89 90 247 224
Labelled Cell Count 151 241 102 240 234 222 412 393 309 327 237 291 209 348 425 168 104 134 144 234 89 90 247 224


Vacuole Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 54.5 61.6 32.7 37.0 37.4 35.7 37.4 36.8 37.0 37.8 34.1 34.0 44.4 45.2 46.9 76.8 69.5 84.9 50.8 53.4 56.5
Std Deviation (1e-4) 17.8 13.5 10.8 11.4 10.3 10.9 11.5 11.6 10.9 11.5 10.8 9.9 13.4 15.4 13.6 22.3 24.3 31.5 14.7 19.6 19.6
Intensity Change (Log2) – – – 0.18 0.19 0.13 0.19 0.17 0.18 0.21 0.06 0.06 0.44 0.47 0.52 1.23 1.09 1.38 0.63 0.71 0.79


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.3 1.6 2.9 3.4 5.1 4.6 5.3 5.6 6.1 0.9 0 0 0 1.2 0.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 4.2 3.5 0 1.9 1.4 0 1.4 1.3 0 2.8 3.7 4.3 2.5 2.8 3.2
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 -1.6 -1.4
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -5.3 -5.4 -5.1 0 0 0 0 0 0 -4.6 -5.2 0 -1.7 -1.9 -1.5
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -1.2 -0.7 -3.5 -3.6 -6.1 -4.4 -5.4 -6.0 -5.7 0 0.5 0.6 -1.3 -0.9 -1.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 31.8915 42.4521 38.4724 36.5461 35.0398 35.5042 32.7727 45.4476 38.2334 37.485 32.506 43.4008 41.8086 48.9628 46.0893 42.0981 43.3732 44.494
Actin 0.007 0 0.0071 0 0.0012 0.0034 0.0152 0 0.0047 0.0001 0.0134 0.0371 0.0012 0 0 0 0 0
Bud 0.0004 0 0.0003 0.0002 0.0002 0.0002 0.0009 0.0001 0.0007 0.0003 0.0012 0.0002 0.0001 0 0.0001 0 0.0007 0
Bud Neck 0.0002 0 0.0002 0 0.0001 0.0003 0.0012 0 0.0001 0.0001 0.0042 0.0001 0.0001 0 0.0001 0 0 0
Bud Periphery 0.0005 0.0001 0.0003 0.0006 0.0003 0.0002 0.0015 0.0002 0.0008 0.0003 0.0008 0.0003 0.0002 0.0001 0.0002 0.0001 0.0028 0.0001
Bud Site 0.0009 0 0.0007 0.0001 0.0001 0.0004 0.0074 0 0.0009 0.0004 0.0028 0.0006 0.0001 0 0.0001 0 0.0001 0
Cell Periphery 0.0021 0.0011 0.0017 0.0017 0.0004 0.0012 0.0046 0.0008 0.0018 0.0015 0.0005 0.0007 0.0019 0.0013 0.002 0.0006 0.004 0.0004
Cytoplasm 0.0178 0.0039 0.0095 0.0006 0.0011 0.0066 0.005 0.0078 0.0017 0.0078 0.0402 0.0002 0.001 0.0014 0.0004 0.0001 0.0001 0.0001
Cytoplasmic Foci 0.0024 0.0001 0.0023 0.0005 0.0002 0.0047 0.014 0.0008 0.0044 0.0066 0.0205 0.0007 0.0012 0 0.0001 0 0 0
Eisosomes 0.0002 0 0 0 0 0 0.0011 0 0 0 0 0.0001 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0046 0.0001 0.0021 0.0003 0.0037 0.0079 0.0041 0.0002 0.001 0.0008 0.0011 0.0003 0.0007 0.0002 0.0002 0 0.0001 0.0001
Endosome 0.0159 0.0026 0.0186 0.0078 0.0075 0.0368 0.0264 0.0116 0.0137 0.0048 0.0312 0.0023 0.0098 0.0032 0.0044 0.0023 0.0016 0.0018
Golgi 0.002 0 0.0035 0.0009 0.0004 0.0111 0.0113 0.0007 0.0091 0.0002 0.0174 0.0018 0.0025 0.0001 0.0003 0 0 0
Lipid Particles 0.0029 0.0002 0.0012 0.0006 0.0004 0.0057 0.007 0.0001 0.0029 0.0032 0.005 0.0012 0.0023 0.0001 0.0004 0.0003 0.0001 0.0001
Mitochondria 0.0107 0.0001 0.0059 0.0013 0.0018 0.0046 0.0093 0.0002 0.0069 0.0041 0.0018 0.0019 0.0033 0.0003 0.0006 0.0001 0.0001 0.0002
None 0.0021 0 0.0002 0 0.0002 0.001 0.0059 0 0.0007 0.0034 0.0009 0.0001 0.0021 0 0 0 0 0
Nuclear Periphery 0.057 0.0089 0.0094 0.002 0.0531 0.0455 0.0319 0.0122 0.0094 0.0043 0.0019 0.0361 0.0156 0.0124 0.0044 0.0011 0.0022 0.0185
Nucleolus 0.0086 0.0007 0.0032 0.0024 0.0005 0.0176 0.009 0.0003 0.0072 0.0038 0.0003 0.0007 0.0134 0.0013 0.0013 0.0004 0.0007 0.0003
Nucleus 0.0102 0.0012 0.0049 0.0015 0.0056 0.0073 0.0067 0.0007 0.0022 0.0036 0.0009 0.0007 0.0044 0.0054 0.001 0.0006 0.0012 0.0009
Peroxisomes 0.0004 0 0.0002 0 0 0.0006 0.001 0 0.0004 0.0003 0.0022 0.0008 0.0004 0 0 0 0 0
Punctate Nuclear 0.0008 0 0.0002 0 0.0002 0.0018 0.0049 0 0.0014 0.0008 0.0039 0.0001 0.002 0 0 0 0 0
Vacuole 0.2783 0.3512 0.3152 0.3419 0.2263 0.1925 0.258 0.2794 0.2985 0.33 0.2234 0.1672 0.2451 0.2619 0.3157 0.272 0.2517 0.207
Vacuole Periphery 0.5749 0.6297 0.6134 0.6375 0.6967 0.6508 0.5736 0.6847 0.6316 0.6236 0.6264 0.7471 0.6926 0.7124 0.6689 0.7222 0.7347 0.7703

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 418.9739 508.9129 434.9171 449.3945 462.3572 581.7636 419.8878 454.6042 511.773 402.9858
Translational Efficiency 3.2687 2.6866 2.7845 2.8075 2.8854 2.1787 2.4519 2.1125 2.1674 2.4581

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Subunit G of the V1 peripheral membrane domain of V-ATPase; part of the electrogenic proton pump found throughout the endomembrane system; involved in vacuolar acidification; the V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase) has eight subunits
Localization
Cell Percentages vacuolar membrane (52%)
Cell Cycle Regulation No
Subcompartmental Group vac memb-2

Vma10

Vma10


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Vma10-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available