Standard name | |
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Human Ortholog | |
Description | Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; general transcription factor involved in telomere maintenance |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 2 | 3 |
Bud | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 2 | 4 | 5 |
Bud Neck | – | 0 | 2 | 0 | 0 | 2 | 1 | 5 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 3 | 2 |
Bud Site | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | – | 0 | 0 | 2 | 2 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 5 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 |
Cytoplasm | – | 13 | 66 | 33 | 37 | 87 | 115 | 147 | 47 | 47 | 27 | 18 | 70 | 113 | 146 | 14 | 30 | 25 | – | 50 | 36 | 47 | 76 | 71 |
Endoplasmic Reticulum | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 3 | 2 | 5 | 3 | – | 0 | 0 | 0 | 0 | 3 |
Endosome | – | 0 | 2 | 0 | 1 | 3 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 3 | 2 | 2 | 11 | 13 | – | 3 | 2 | 0 | 1 | 2 |
Golgi | – | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 0 | 0 | – | 0 | 1 | 0 | 0 | 2 |
Mitochondria | – | 10 | 0 | 0 | 0 | 11 | 10 | 25 | 35 | 22 | 36 | 42 | 0 | 0 | 0 | 4 | 20 | 7 | – | 5 | 15 | 2 | 2 | 12 |
Nucleus | – | 0 | 3 | 4 | 3 | 1 | 3 | 7 | 1 | 4 | 0 | 2 | 4 | 2 | 1 | 30 | 50 | 38 | – | 2 | 6 | 5 | 7 | 6 |
Nuclear Periphery | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 1 |
Nucleolus | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 2 | 1 | 0 | 0 | 0 | 1 | 4 | 1 | – | 0 | 1 | 1 | 1 | 2 |
Peroxisomes | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
SpindlePole | – | 0 | 3 | 0 | 0 | 0 | 1 | 5 | 2 | 3 | 1 | 0 | 0 | 1 | 0 | 1 | 2 | 3 | – | 1 | 6 | 4 | 3 | 14 |
Vac/Vac Membrane | – | 3 | 5 | 3 | 6 | 11 | 3 | 12 | 4 | 3 | 2 | 1 | 6 | 6 | 10 | 3 | 18 | 17 | – | 3 | 3 | 4 | 9 | 15 |
Unique Cell Count | 21 | 79 | 40 | 46 | 106 | 125 | 179 | 74 | 74 | 57 | 51 | 83 | 126 | 157 | 51 | 114 | 90 | 70 | 82 | 75 | 120 | 153 | ||
Labelled Cell Count | 26 | 83 | 43 | 49 | 116 | 135 | 203 | 92 | 81 | 69 | 66 | 83 | 127 | 169 | 58 | 141 | 108 | 70 | 82 | 75 | 120 | 153 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | – | 3.4 | 5.0 | 4.7 | 5.8 | 4.2 | 4.1 | 4.7 | 3.6 | 4.1 | 4.0 | 3.7 | 5.3 | 5.4 | 5.1 | 4.9 | 5.9 | 5.6 | 6.4 | 5.4 | 6.4 |
Std Deviation (1e-4) | – | 1.1 | 1.5 | 1.0 | 2.2 | 1.2 | 1.4 | 2.0 | 2.0 | 1.6 | 1.9 | 1.3 | 1.9 | 1.7 | 1.8 | 2.0 | 2.6 | 1.8 | 2.8 | 2.1 | 3.1 |
Intensity Change (Log2) | – | – | – | -0.11 | 0.21 | -0.25 | -0.29 | -0.1 | -0.47 | -0.31 | -0.34 | -0.45 | 0.07 | 0.09 | 0.02 | -0.04 | 0.24 | 0.15 | 0.34 | 0.09 | 0.35 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | 2.0 | 2.4 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Actin | 0.2766 | 0.0327 | 0.0026 | 0.1921 | 0.3616 | 0.0008 | – | – | – | – | – | – | 0.0428 | 0.0002 | 0.013 | 0.0008 | 0.0004 | 0.0222 |
Bud | 0.0023 | 0.001 | 0.0033 | 0.0012 | 0.0004 | 0.0001 | – | – | – | – | – | – | 0.0006 | 0.0001 | 0.0006 | 0.0005 | 0.0002 | 0.0007 |
Bud Neck | 0.0502 | 0.0015 | 0.0009 | 0.0024 | 0.0029 | 0.0096 | – | – | – | – | – | – | 0.0077 | 0.0005 | 0.0009 | 0.0019 | 0.0033 | 0.0292 |
Bud Periphery | 0.004 | 0.0016 | 0.0025 | 0.0012 | 0.0005 | 0.0001 | – | – | – | – | – | – | 0.0008 | 0.0001 | 0.0009 | 0.0008 | 0.0002 | 0.0014 |
Bud Site | 0.0128 | 0.0049 | 0.0044 | 0.007 | 0.001 | 0.0001 | – | – | – | – | – | – | 0.0145 | 0.001 | 0.0008 | 0.0014 | 0.0005 | 0.0011 |
Cell Periphery | 0.0024 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0 | – | – | – | – | – | – | 0.0006 | 0.0001 | 0.0002 | 0.0003 | 0 | 0.0002 |
Cytoplasm | 0.0151 | 0.0663 | 0.0607 | 0.0417 | 0.0124 | 0.1095 | – | – | – | – | – | – | 0.0718 | 0.1056 | 0.0759 | 0.1376 | 0.1218 | 0.1307 |
Cytoplasmic Foci | 0.0441 | 0.0082 | 0.0096 | 0.023 | 0.0133 | 0.0037 | – | – | – | – | – | – | 0.0201 | 0.0069 | 0.0243 | 0.0152 | 0.0014 | 0.0449 |
Eisosomes | 0.003 | 0.0003 | 0.0001 | 0.0005 | 0.0011 | 0.0002 | – | – | – | – | – | – | 0.0005 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0002 |
Endoplasmic Reticulum | 0.0101 | 0.001 | 0.0033 | 0.0002 | 0.0084 | 0.0014 | – | – | – | – | – | – | 0.003 | 0.0026 | 0.003 | 0.0031 | 0.0025 | 0.0039 |
Endosome | 0.097 | 0.0014 | 0.0105 | 0.0024 | 0.0296 | 0.0016 | – | – | – | – | – | – | 0.0149 | 0.0026 | 0.0034 | 0.0644 | 0.0018 | 0.0128 |
Golgi | 0.0332 | 0.0025 | 0.0016 | 0.0159 | 0.0214 | 0.0001 | – | – | – | – | – | – | 0.0047 | 0.0002 | 0.0009 | 0.0077 | 0.0001 | 0.0099 |
Lipid Particles | 0.06 | 0.0011 | 0.0012 | 0.0207 | 0.0134 | 0.0004 | – | – | – | – | – | – | 0.0161 | 0.0032 | 0.0006 | 0.0061 | 0.0001 | 0.0068 |
Mitochondria | 0.021 | 0.0143 | 0.0058 | 0.009 | 0.0018 | 0.0004 | – | – | – | – | – | – | 0.0046 | 0.0008 | 0.0013 | 0.0059 | 0.0007 | 0.0323 |
None | 0.22 | 0.7185 | 0.5676 | 0.2785 | 0.4975 | 0.4463 | – | – | – | – | – | – | 0.2648 | 0.4672 | 0.3608 | 0.4054 | 0.3764 | 0.3563 |
Nuclear Periphery | 0.0228 | 0.0037 | 0.0344 | 0.0011 | 0.0123 | 0.011 | – | – | – | – | – | – | 0.0223 | 0.012 | 0.009 | 0.0107 | 0.0254 | 0.0132 |
Nucleolus | 0.0095 | 0.0031 | 0.0038 | 0.0005 | 0.0004 | 0.0049 | – | – | – | – | – | – | 0.0088 | 0.0079 | 0.0076 | 0.0052 | 0.0094 | 0.0073 |
Nucleus | 0.0093 | 0.117 | 0.2127 | 0.3438 | 0.0013 | 0.3622 | – | – | – | – | – | – | 0.4101 | 0.3394 | 0.4121 | 0.1949 | 0.3453 | 0.279 |
Peroxisomes | 0.0394 | 0.0016 | 0.0011 | 0.0566 | 0.0059 | 0.0001 | – | – | – | – | – | – | 0.005 | 0.0003 | 0.0002 | 0.0014 | 0.0001 | 0.0039 |
Punctate Nuclear | 0.0236 | 0.0181 | 0.0682 | 0.0019 | 0.0136 | 0.0468 | – | – | – | – | – | – | 0.08 | 0.0467 | 0.0816 | 0.1117 | 0.1078 | 0.04 |
Vacuole | 0.0365 | 0.0009 | 0.0035 | 0.0002 | 0.0004 | 0.0007 | – | – | – | – | – | – | 0.0051 | 0.0019 | 0.0024 | 0.0195 | 0.0023 | 0.0029 |
Vacuole Periphery | 0.0072 | 0.0002 | 0.0022 | 0.0001 | 0.0008 | 0.0002 | – | – | – | – | – | – | 0.0013 | 0.0004 | 0.0006 | 0.0054 | 0.0004 | 0.0011 |
R1 | R2 | |||||||||
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G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 44.4499 | 51.4225 | 54.3458 | 32.9654 | 38.4209 | 64.4603 | 43.7215 | 43.599 | 58.006 | 43.0006 |
Translational Efficiency | 0.4862 | 0.4028 | 0.3769 | 0.7953 | 0.7189 | 0.3363 | 0.4389 | 0.6168 | 0.4112 | 0.5059 |
Dataset | Hit |
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Protein Concentration | – |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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